BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0011.p (474 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKZ6.1 B3KKZ6_9BACI 3-isopropylmalate dehydratase, large subun... 889 0.0 B1DL79.1 B1DL79_9BACL 3-isopropylmalate dehydratase, large subun... 854 0.0 C0ZCK1.1 C0ZCK1_BREBN 3-isopropylmalate dehydratase large subuni... 752 0.0 A3ZQJ4.1 A3ZQJ4_9PLAN Isopropylmalate isomerase large subunit EC... 689 0.0 Q67MJ2.1 LEUC_SYMTH 3-isopropylmalate dehydratase large subunit ... 678 0.0 >B3KKZ6.1 B3KKZ6_9BACI 3-isopropylmalate dehydratase, large subunit EC=4.2.1.33 Length = 484 Score = 889 bits (2297), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/472 (90%), Positives = 449/472 (95%) Query: 1 MGNKTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFA 60 M KTMFEKIWDNHVIHQEEGKP I+YIDLHLVHEVTSPQAFEGLRLSGRKVRRP LTFA Sbjct: 11 MSKKTMFEKIWDNHVIHQEEGKPGILYIDLHLVHEVTSPQAFEGLRLSGRKVRRPELTFA 70 Query: 61 TMDHNVPTKDRYNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHP 120 TMDHNVPTKDR+NITDPISKQQIDTL++NC+DFGV L+DL+ IDQGVVHVMGPE+GLTHP Sbjct: 71 TMDHNVPTKDRFNITDPISKQQIDTLTKNCQDFGVTLYDLDTIDQGVVHVMGPELGLTHP 130 Query: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTA 180 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQ+KAKTMEVRF G+RNPGVTA Sbjct: 131 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQAKAKTMEVRFVGRRNPGVTA 190 Query: 181 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTF 240 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAG+IAPDETTF Sbjct: 191 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGMIAPDETTF 250 Query: 241 NYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 YLRGRQYVPQG A++AAV WKELV+DEGA++D VVEFDVETLIPQVTWGTSPGMGT+I Sbjct: 251 EYLRGRQYVPQGEAFDAAVAAWKELVTDEGAEFDVVVEFDVETLIPQVTWGTSPGMGTDI 310 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 +STVPNPADF TENERKAAEKALEYM LVPGTP+SEI IDYVFIGSCTNGRIEDLRAAA Sbjct: 311 SSTVPNPADFKTENERKAAEKALEYMALVPGTPMSEIPIDYVFIGSCTNGRIEDLRAAAA 370 Query: 361 VAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 420 VAKGHKVS VTAIVVPGSGRVK+QAEKEGLDK+F EAGFEWREAGCSMCLAMNPDVL+P Sbjct: 371 VAKGHKVSSNVTAIVVPGSGRVKIQAEKEGLDKIFVEAGFEWREAGCSMCLAMNPDVLKP 430 Query: 421 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVRDWNYKAEAV 472 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAA+KG F DVRDWN+ AE V Sbjct: 431 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVKGRFVDVRDWNFAAEEV 482 >B1DL79.1 B1DL79_9BACL 3-isopropylmalate dehydratase, large subunit EC=4.2.1.33 Length = 471 Score = 854 bits (2206), Expect = 0.0, Method: Compositional matrix adjust. Identities = 410/473 (86%), Positives = 441/473 (93%), Gaps = 2/473 (0%) Query: 1 MGNKTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFA 60 M +T+FEKIWD HVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLS RKVRRP LTFA Sbjct: 1 MTKRTLFEKIWDKHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSNRKVRRPDLTFA 60 Query: 61 TMDHNVPTKDRYNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHP 120 TMDHNVPTKDR+NI DPISKQQIDTL+QNC DFGVKLFDLN++DQGVVHVMGPEIGLT+P Sbjct: 61 TMDHNVPTKDRFNIKDPISKQQIDTLTQNCADFGVKLFDLNNLDQGVVHVMGPEIGLTYP 120 Query: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTA 180 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHV+ATQCLQQ+K KTMEVRF G R PGVTA Sbjct: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQCLQQAKPKTMEVRFNGSRKPGVTA 180 Query: 181 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTF 240 KD+ILGVIAKYGTDFATGYVIEYTGEAIR L+MEERMTVCNMSIEGGARAGLIAPD+TTF Sbjct: 181 KDLILGVIAKYGTDFATGYVIEYTGEAIRSLTMEERMTVCNMSIEGGARAGLIAPDQTTF 240 Query: 241 NYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 +YL+GR+Y P A Y+ AVE WK+L +DEGA YD VV+FDV++LIPQVTWGTSPGMGT+I Sbjct: 241 DYLKGREYAP--ANYDEAVEDWKQLTTDEGAAYDVVVDFDVDSLIPQVTWGTSPGMGTDI 298 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 TS+VP P D TENERKAAEKALEYMDL PGTP+SEI IDYVFIGSCTNGRIEDLRAAAE Sbjct: 299 TSSVPFPKDLPTENERKAAEKALEYMDLKPGTPMSEIEIDYVFIGSCTNGRIEDLRAAAE 358 Query: 361 VAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 420 +A+G+KVS+KVTAIVVPGSGRVK+QAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP Sbjct: 359 IARGYKVSEKVTAIVVPGSGRVKLQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 418 Query: 421 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVRDWNYKAEAVN 473 GQRCASTSNRNFEGRQGRGGRTHLVSPAMA AAAI G FTDVRDWN K+E +N Sbjct: 419 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAVAAAINGRFTDVRDWNVKSEVLN 471 >C0ZCK1.1 C0ZCK1_BREBN 3-isopropylmalate dehydratase large subunit EC=4.2.1.33 Length = 476 Score = 752 bits (1941), Expect = 0.0, Method: Compositional matrix adjust. Identities = 356/466 (76%), Positives = 401/466 (86%) Query: 1 MGNKTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFA 60 M +TMFEKIWDNH IH+E GKPS++YIDLHLVHEVTSPQAFEGLRL+GRKVRRP LTFA Sbjct: 1 MTARTMFEKIWDNHAIHEEAGKPSLLYIDLHLVHEVTSPQAFEGLRLAGRKVRRPELTFA 60 Query: 61 TMDHNVPTKDRYNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHP 120 TMDHNVPT DR+N+ DPIS+QQ++TL+ NC +FG+ L DLN DQG+VHV+GPE+GLTHP Sbjct: 61 TMDHNVPTADRFNVKDPISRQQMETLTANCEEFGITLADLNSPDQGIVHVIGPELGLTHP 120 Query: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTA 180 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSK KTMEVR G GV+A Sbjct: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKPKTMEVRVNGDLPFGVSA 180 Query: 181 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTF 240 KD+IL +IAKYGTDFATGYV+EYTG AIR L+MEERMTVCNM+IEGGARAGLIAPD+ TF Sbjct: 181 KDLILAIIAKYGTDFATGYVVEYTGAAIRNLTMEERMTVCNMTIEGGARAGLIAPDQITF 240 Query: 241 NYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 +YL+G+++VPQG + AAVE WKEL +DEGA +D VE + + PQVTWGTSPGMGTNI Sbjct: 241 DYLKGKRFVPQGEDFLAAVEAWKELCTDEGAVFDACVEIEAAEIAPQVTWGTSPGMGTNI 300 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 TSTVP+P F T +RKAA+ AL YMDL PGTP+SEI ID VFIGSCTNGRIEDLR AAE Sbjct: 301 TSTVPSPESFETVAQRKAAQDALAYMDLAPGTPMSEIKIDRVFIGSCTNGRIEDLRKAAE 360 Query: 361 VAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 420 VAKG KVS V A+VVPGS VK AE+EGL +F EAGF+WRE+GCSMCLAMNPD+L Sbjct: 361 VAKGRKVSPSVHAMVVPGSQAVKQLAEQEGLHLIFQEAGFDWRESGCSMCLAMNPDILSE 420 Query: 421 GQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVRDWN 466 G+RCASTSNRNFEGRQGRGGRTHLVSP MAAAAAI GHF DVR+W Sbjct: 421 GERCASTSNRNFEGRQGRGGRTHLVSPEMAAAAAIAGHFVDVREWK 466 >A3ZQJ4.1 A3ZQJ4_9PLAN Isopropylmalate isomerase large subunit EC=4.2.1.33 Length = 470 Score = 689 bits (1777), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/466 (69%), Positives = 383/466 (82%), Gaps = 3/466 (0%) Query: 4 KTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFATMD 63 + MF+KIWDNH++ QEEG+ +I+YIDL LVHEVTS QAFEGLR++GRK+RRP AT D Sbjct: 7 RNMFQKIWDNHLVLQEEGQQAILYIDLQLVHEVTSAQAFEGLRIAGRKLRRPERHIATPD 66 Query: 64 HNVPTKDR-YNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHPGK 122 HN+PT DR I DPISKQQIDTL NC +FGV+L+DLND QG+VHV+GPE+G+T PG Sbjct: 67 HNIPTTDRSLPIADPISKQQIDTLRNNCVEFGVRLYDLNDSKQGIVHVIGPELGMTQPGM 126 Query: 123 TIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTAKD 182 TIVCGDSHT+THGAFGALAFGIGTSEVEHV+ATQ L Q+K KTME+R G PGVTAKD Sbjct: 127 TIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLLQAKPKTMELRVNGTLAPGVTAKD 186 Query: 183 MILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTFNY 242 +IL +I K T TGYV+EYTGEAIR LSMEERMTVCNMSIE GARAG+IAPDETTF Y Sbjct: 187 LILYLIGKLTTAGGTGYVLEYTGEAIRALSMEERMTVCNMSIEAGARAGMIAPDETTFAY 246 Query: 243 LRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNITS 302 ++GR++ P+ ++AAVE WK+L SDEGA+YD V+ FD + + PQ+TWGT+PG + S Sbjct: 247 VKGREFAPKD--FDAAVEKWKQLPSDEGAKYDEVLIFDAKDIAPQITWGTNPGQVAPVIS 304 Query: 303 TVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAEVA 362 VP+P+D+ E+K+ + AL+YM L G P++E+ ID VFIGSCTN RIEDLRAAA+V Sbjct: 305 KVPSPSDYGDATEQKSTQAALDYMGLTAGQPLTEVKIDRVFIGSCTNARIEDLRAAAKVV 364 Query: 363 KGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQPGQ 422 KGHKV+ V+A+VVPGSG+VK QAE EGLD VF EAGFEWREAGCSMCLAMNPD L PG+ Sbjct: 365 KGHKVASGVSAMVVPGSGQVKDQAEAEGLDAVFREAGFEWREAGCSMCLAMNPDKLAPGE 424 Query: 423 RCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVRDWNYK 468 RCASTSNRNFEGRQG+GGRTHLVSPAMAAAA + GHF D+RDW Y+ Sbjct: 425 RCASTSNRNFEGRQGKGGRTHLVSPAMAAAAGVTGHFVDIRDWTYQ 470 >Q67MJ2.1 LEUC_SYMTH 3-isopropylmalate dehydratase large subunit EC=4.2.1.33 Length = 481 Score = 678 bits (1750), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/465 (70%), Positives = 377/465 (81%), Gaps = 1/465 (0%) Query: 1 MGNKTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFA 60 M +T+ +KIW+ HV+ +E GKP ++YIDLHLVHEVTSPQAFEGLR++GR+VRRP LT A Sbjct: 8 MSGRTLLDKIWERHVVRREPGKPDLLYIDLHLVHEVTSPQAFEGLRMAGRRVRRPDLTIA 67 Query: 61 TMDHNVPTKDR-YNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTH 119 TMDHNVPT DR +TD I+ +Q+ L +NC +FGV+LFDL QG+VHV+GPE+GLT Sbjct: 68 TMDHNVPTTDRSLPLTDEIAARQMAALERNCSEFGVRLFDLYSPFQGIVHVIGPELGLTQ 127 Query: 120 PGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVT 179 PG TIVCGDSHT+THGAFGALAFGIGTSEVEHVLATQCL Q K K ME+R GK PG T Sbjct: 128 PGLTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLWQHKPKVMEIRVNGKLPPGTT 187 Query: 180 AKDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETT 239 AKD+ILG+I + GTD ATGYVIEYTGEAIR LSME RMTVCNMSIE GARAG+IAPDETT Sbjct: 188 AKDLILGIIGQIGTDGATGYVIEYTGEAIRSLSMEGRMTVCNMSIEAGARAGMIAPDETT 247 Query: 240 FNYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTN 299 F YL+GR + PQG +EAAV W+ L SD A YD VVEFD L P V+WGT+PG Sbjct: 248 FAYLKGRPHAPQGELWEAAVADWRTLASDPDAVYDRVVEFDAGQLAPVVSWGTNPGQVVP 307 Query: 300 ITSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAA 359 +T +P+P DF +RKAAE AL YMDL PGTPI +I ID VFIGSCTNGRIEDLRAAA Sbjct: 308 VTGRIPDPRDFADPAQRKAAEAALAYMDLEPGTPIQDIRIDRVFIGSCTNGRIEDLRAAA 367 Query: 360 EVAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQ 419 V KG KV+ V A+VVPGSG+VK QAE EGLD++F EAGFEWREAGCSMCL MNPD+L Sbjct: 368 AVVKGRKVAPGVRAMVVPGSGQVKAQAEAEGLDQIFREAGFEWREAGCSMCLGMNPDILA 427 Query: 420 PGQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVRD 464 PG+RCASTSNRNFEGRQG+GGRTHLVSPAMAAAAAI GHF DVR+ Sbjct: 428 PGERCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAIAGHFVDVRE 472 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,947,613,579 Number of extensions: 125853142 Number of successful extensions: 297962 Number of sequences better than 1.0e-05: 3945 Number of HSP's gapped: 291304 Number of HSP's successfully gapped: 4178 Length of query: 474 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 334 Effective length of database: 1,741,754,103 Effective search space: 581745870402 Effective search space used: 581745870402 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)