BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0010.p (208 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKZ7.1 B3KKZ7_9BACI 3-isopropylmalate dehydratase, small subun... 338 1e-91 B1DL78.1 B1DL78_9BACL 3-isopropylmalate dehydratase, small subun... 323 7e-87 Q9LCR5.1 LEUD_PAEPO 3-isopropylmalate dehydratase small subunit ... 303 6e-81 C0ZCK0.1 LEUD_BREBN 3-isopropylmalate dehydratase small subunit ... 279 1e-73 Q67MJ3.2 LEUD_SYMTH 3-isopropylmalate dehydratase small subunit ... 261 3e-68 >B3KKZ7.1 B3KKZ7_9BACI 3-isopropylmalate dehydratase, small subunit EC=4.2.1.33 Length = 200 Score = 338 bits (868), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 157/200 (78%), Positives = 177/200 (88%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEM 68 M+AF KL GIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDE G + +F + Sbjct: 1 MQAFTKLNGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEEGGNIDSFSL 60 Query: 69 NKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIK 128 N+PRY+G++ILISRANFGCGS REHAPWAI+DYGFRVVIAPS+ADIFYNNCFKNGILPIK Sbjct: 61 NQPRYQGSTILISRANFGCGSSREHAPWAILDYGFRVVIAPSFADIFYNNCFKNGILPIK 120 Query: 129 LSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLT 188 LSE QV+ELF+RT ++GYKL VDLEN ++DD GL I F+LDEHRRQFLLQGLDDIGLT Sbjct: 121 LSEEQVEELFQRTESNEGYKLNVDLENKTITDDQGLHIDFDLDEHRRQFLLQGLDDIGLT 180 Query: 189 LQHADEIAAYEERHAAKLFA 208 LQH I AYE++H +LFA Sbjct: 181 LQHESAIDAYEKKHGERLFA 200 >B1DL78.1 B1DL78_9BACL 3-isopropylmalate dehydratase, small subunit EC=4.2.1.33 Length = 199 Score = 323 bits (827), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 160/198 (80%), Positives = 182/198 (91%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEM 68 MEAFK+LTGIVAPVDRVNVDTDAIIPKQFLKRIER+GFGQFLF+EWRF E G NA FE Sbjct: 1 MEAFKQLTGIVAPVDRVNVDTDAIIPKQFLKRIERSGFGQFLFFEWRFHENGEVNADFEP 60 Query: 69 NKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIK 128 NKPRY+GAS+LISRANFGCGS REHAPWAI+DYGF+VVIAPSYADIFYNNCFKNGILPIK Sbjct: 61 NKPRYQGASVLISRANFGCGSSREHAPWAILDYGFKVVIAPSYADIFYNNCFKNGILPIK 120 Query: 129 LSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLT 188 LSE QV++LF+RTA+H+GYKL+VDLEN L+D++GL ++F+LDEHRRQFLL GLDDIGLT Sbjct: 121 LSEEQVEDLFQRTAKHEGYKLSVDLENKKLTDEYGLDLSFDLDEHRRQFLLLGLDDIGLT 180 Query: 189 LQHADEIAAYEERHAAKL 206 +QHADEI AYE++ A L Sbjct: 181 MQHADEITAYEQKRPAYL 198 >Q9LCR5.1 LEUD_PAEPO 3-isopropylmalate dehydratase small subunit EC=4.2.1.33 (Fragment) Length = 178 Score = 303 bits (776), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 142/175 (81%), Positives = 155/175 (88%) Query: 31 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEMNKPRYKGASILISRANFGCGSP 90 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGN N FE NKPRY GAS+LISRANFGCGS Sbjct: 1 AIIPKQFLKRIERTGFGQFLFYEWRFDEAGNINPEFEPNKPRYAGASVLISRANFGCGSS 60 Query: 91 REHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIKLSESQVDELFKRTAEHDGYKLT 150 REHAPWAIMDYGFR VIAPS+ADIFYNNCFKNGILPIKLSE QV++LF+RTA+HD Y+L Sbjct: 61 REHAPWAIMDYGFRCVIAPSFADIFYNNCFKNGILPIKLSEEQVEDLFQRTAKHDNYQLN 120 Query: 151 VDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLTLQHADEIAAYEERHAAK 205 VDL ++DD+GL I F+LDEHRRQFLLQGLDDIGLTLQH EI AYE++ AAK Sbjct: 121 VDLNEKTITDDYGLRIEFDLDEHRRQFLLQGLDDIGLTLQHESEILAYEQKRAAK 175 >C0ZCK0.1 LEUD_BREBN 3-isopropylmalate dehydratase small subunit EC=4.2.1.33 Length = 194 Score = 279 bits (714), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 130/194 (67%), Positives = 154/194 (79%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEM 68 M F TG+VAP+DRVNVDTDAIIPKQFLKRIER+GFGQFLFYEWRF G +F + Sbjct: 1 MNPFVIHTGVVAPLDRVNVDTDAIIPKQFLKRIERSGFGQFLFYEWRFTVDGAPIDSFIL 60 Query: 69 NKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIK 128 N P YK +++L++R NFGCGS REHAPWA++DYGFR VIAPS+ADIFYNNCFKNGILPIK Sbjct: 61 NTPAYKESTVLLARNNFGCGSSREHAPWALLDYGFRCVIAPSFADIFYNNCFKNGILPIK 120 Query: 129 LSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLT 188 LSE QVDELF R Y+LT+DL+ ++ D+ GLS FE+D +RR LL GLDDIG+T Sbjct: 121 LSEEQVDELFNRAESKPNYQLTIDLQEQVVRDNEGLSYPFEVDSYRRYCLLNGLDDIGIT 180 Query: 189 LQHADEIAAYEERH 202 LQ+ D+IAAYE R Sbjct: 181 LQYEDKIAAYEARR 194 >Q67MJ3.2 LEUD_SYMTH 3-isopropylmalate dehydratase small subunit EC=4.2.1.33 Length = 203 Score = 261 bits (666), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 123/198 (62%), Positives = 150/198 (75%), Gaps = 1/198 (0%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEM 68 M F + TG+ P+DRVNVDTD IIPKQFLKRIERTGFGQFLF++WR+ G+ N F + Sbjct: 1 MRPFTRETGLAVPLDRVNVDTDQIIPKQFLKRIERTGFGQFLFHDWRYLPDGSPNPEFVL 60 Query: 69 NKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIK 128 N+P+Y GA+ILI+ NFG GS REHAPWA+ DYGFR +IAPS+ADIFYNNCF+NG+LP+ Sbjct: 61 NRPQYAGATILIAGRNFGSGSSREHAPWALSDYGFRAIIAPSFADIFYNNCFQNGLLPVV 120 Query: 129 LSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLT 188 L E V EL +R A+ GY+LTVDLE ++ DD G + F +D RR +L GLDDIGLT Sbjct: 121 LPEEAVAELMRR-AQEPGYRLTVDLERCVVEDDAGFRVDFAIDAFRRHRMLHGLDDIGLT 179 Query: 189 LQHADEIAAYEERHAAKL 206 LQ+ DEIAAYE R A L Sbjct: 180 LQYEDEIAAYEARRPAWL 197 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,251,055,252 Number of extensions: 52652019 Number of successful extensions: 119737 Number of sequences better than 1.0e-05: 1542 Number of HSP's gapped: 117904 Number of HSP's successfully gapped: 1542 Length of query: 208 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 78 Effective length of database: 1,835,964,253 Effective search space: 143205211734 Effective search space used: 143205211734 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)