BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0008.p (286 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K710.1 B3K710_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.... 230 1e-58 Q9LCR4.1 Q9LCR4_PAEPO CwlU 218 4e-55 B1DL77.1 B1DL77_9BACL N-acetylmuramoyl-L-alanine amidase EC=3.5.... 212 3e-53 B3K708.1 B3K708_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.... 199 2e-49 Q9LCR3.1 Q9LCR3_PAEPO CwlV 187 1e-45 >B3K710.1 B3K710_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 478 Score = 230 bits (586), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 122/285 (42%), Positives = 178/285 (62%), Gaps = 11/285 (3%) Query: 1 MGSPDRIVIDFPGAVFAPDFAGSFPGVSTSGSPQGKLDVSGYPLVTEVRYALFSVSPPTA 60 M P RIVID P V++ F G + + G ++ + Y VT +RYA+FS +PPT Sbjct: 201 MTGPARIVIDLPDTVYSASF---LSGHAVNAQNGGSMEAANYSDVTNIRYAVFSDNPPTV 257 Query: 61 RFVIQTVGSQPYQVSKDDSTGLVTVDLNAVSSXXXXXXX-XXXXXKPVVVLDAGHGGTQS 119 R V+ G + Y V+++ +G++ VDLNA K VV+D GHGG + Sbjct: 258 RIVLDLTGPKNYAVTQE--SGIIIVDLNAEGEILVPPVPPVTGNGKKTVVIDPGHGGIKP 315 Query: 120 GAVSLTGKLEKNFNLAVIQKAGALLMQAGLVDVIFTRTEDVTRGLQERVDIAERAKANLF 179 G++SLTGK EK+FNL + K ALL + ++V+ TR++D L +RV IA KA++F Sbjct: 316 GSISLTGKTEKSFNLDISLKVEALLKKEKNINVVMTRSDDSHLELSDRVKIANNLKADIF 375 Query: 180 ISVHGNSLPADYPNRNKVNGSETYYSRAESLPLAQIMHKHLVAATGFKDNGIRSKSLHVT 239 +S+HGNS + P+ G+ETYY+R S LA ++HKHL ATG KD G++ SLHVT Sbjct: 376 VSIHGNSNASSSPS-----GTETYYTRDSSKSLADVIHKHLAKATGLKDRGVKYGSLHVT 430 Query: 240 RESSMPAVLLEVGYLTNPGNESAMYSEQLQDSLAREIVAGIMEYL 284 RE++MPAVLLE+G+L+N G+E+ ++ + Q+ +A+ IV GI EYL Sbjct: 431 RETTMPAVLLEIGFLSNKGDEAQLFKDDFQNRVAQGIVEGIKEYL 475 >Q9LCR4.1 Q9LCR4_PAEPO CwlU Length = 524 Score = 218 bits (556), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 127/285 (44%), Positives = 166/285 (58%), Gaps = 6/285 (2%) Query: 1 MGSPDRIVIDFPGAVFAPDFAGSFPGVSTSGSPQGKLDVSGYPLVTEVRYALFSVSPPTA 60 M PDRIV+D P A + D SFP V G+ L +S V E+R+A + A Sbjct: 236 MTGPDRIVVDLPEASLSADLLQSFP-VRKDGT--RALTISDSEDVQEIRFAPADGTKGGA 292 Query: 61 RFVIQTVGSQPYQVSKDDSTGLVTVDLNAVSSXXXXXXXXXXXXKPVVVLDAGHGGTQSG 120 R VI ++ Y +S + G +T+ L S + +VV+D GHGG G Sbjct: 293 RIVIALNQTRDYDLSTN-GVGDITLQLRERSVNPEIVAPTYVEGRRIVVIDPGHGGKDPG 351 Query: 121 AVSLTGKLEKNFNLAVIQKAGALLMQAGLVDVIFTRTEDVTRGLQERVDIAERAKANLFI 180 A S+TG+ EK F LAV K L + ++ TR D L ER +A +A++F+ Sbjct: 352 AGSVTGRHEKEFTLAVALKVQQLAQNDPDIQIVLTRNGDTYPTLDERPQLANNQQASVFV 411 Query: 181 SVHGNSLPADYPNRNKVNGSETYYSRAESLPLAQIMHKHLVAATGFKDNGIRSKSLHVTR 240 S+HGNS+P N K NGSETYY+R ESL LA IMHKHLVAAT FKDNGI+ + VTR Sbjct: 412 SIHGNSMPTS--NNGKANGSETYYARQESLSLATIMHKHLVAATEFKDNGIKVANHVVTR 469 Query: 241 ESSMPAVLLEVGYLTNPGNESAMYSEQLQDSLAREIVAGIMEYLG 285 +S MPAVLLE GYL+NP NE+AM++E+ Q +A IV G+ EYLG Sbjct: 470 KSQMPAVLLECGYLSNPSNEAAMFTEETQQKIAEGIVDGLKEYLG 514 >B1DL77.1 B1DL77_9BACL N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 473 Score = 212 bits (540), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 118/293 (40%), Positives = 168/293 (57%), Gaps = 18/293 (6%) Query: 1 MGSPDRIVIDFPGAVFAPDFAGSFPGVSTSGSPQGKLDVSGYPLVTEVRYALFSVSPPTA 60 + SP RIVIDFP ++ + + F G T ++ V+ P ++ RY++FS +P TA Sbjct: 191 LDSPKRIVIDFPNTTYSDNLSSQFMGAET------RIPVADNPYISMFRYSVFSTNPATA 244 Query: 61 RFVIQ-TVGSQPYQVSKDDSTGLVTVDLNAVSSXXXXXXXXXXXXKPV------VVLDAG 113 R V+ V +D+ G++T+ L + VV+DAG Sbjct: 245 RLVLDLNTDKDADAVVQDNGAGVITIGLTDPVTTPPVDTTTPPVTTDPGTKVYNVVIDAG 304 Query: 114 HGGTQSGAVSLTGKLEKNFNLAVIQKAGALLMQAGLVDVIFTRTEDVTRGLQERVDIAER 173 HGGT GA S+ G+ EK NLAV K ALL + + + +R ED L +RV A+ Sbjct: 305 HGGTDPGAQSINGRWEKEVNLAVSLKVKALLDKEKNIKPLLSRPEDKFVTLADRVTFAKN 364 Query: 174 AKANLFISVHGNSLPADYPNRNKVNGSETYYSRAESLPLAQIMHKHLVAATGFKDNGIRS 233 KA++FIS+H NS P + V G+ETYY+R S LA ++HKHLV ATG KD G++ Sbjct: 365 NKADIFISIHANSNPT-----SSVTGTETYYTRDSSKALANVIHKHLVKATGLKDRGVKY 419 Query: 234 KSLHVTRESSMPAVLLEVGYLTNPGNESAMYSEQLQDSLAREIVAGIMEYLGL 286 +LHVTRE++MPA+LLE G+L+N G+ +YS+ Q+ +A EIVAGI EYL L Sbjct: 420 GNLHVTRETTMPAILLETGFLSNKGDSDILYSDAAQNKMAAEIVAGIKEYLKL 472 >B3K708.1 B3K708_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 461 Score = 199 bits (507), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 117/287 (40%), Positives = 164/287 (57%), Gaps = 16/287 (5%) Query: 1 MGSPDRIVIDFPGAVFAPDFAGS--FPGVSTSGSPQGKLDVSGYPLVTEVRYALFSVSPP 58 + SPDRIVID F+ F+ + F V+ SG ++ VSGYP V VRY+L++ +P Sbjct: 185 LSSPDRIVIDMANTNFSETFSSNADFDDVAKSG----QMTVSGYPDVKAVRYSLYNSNPM 240 Query: 59 TARFVIQTVGSQPYQVSK-DDSTGLVTVDLNAVSSXXXXXXXXXXXXKPVVVLDAGHGGT 117 T R VI ++ Y VS ++ +GL VDLN S+ K +VV+DAGHG Sbjct: 241 TVRIVIDLNYAKNYTVSGLENGSGLFIVDLNTTSNPSPEPGTGG---KKLVVIDAGHGDH 297 Query: 118 QSGAVSLTGKLEKNFNLAVIQKAGALLMQAGLVDVIFTRTEDVTRGLQERVDIAERAKAN 177 GA+ +T K EK+FNLA+ K LL + ++V+ TR+ D L++R IA KA+ Sbjct: 298 DPGAIGVTKKREKDFNLAMALKVEKLLAKEKNIEVVLTRSNDTFLELKDRAKIANDLKAD 357 Query: 178 LFISVHGNSLPADYPNRNKVNGSETYYSRAESLPLAQIMHKHLVAATGFKDNGIRSKSLH 237 +FIS+H NS P+ +G+ETYY LA +HK +V A G KD G+R + H Sbjct: 358 VFISIHANSGPS------TASGTETYYYNESDKALANALHKEIVKAMGLKDRGVRYGNFH 411 Query: 238 VTRESSMPAVLLEVGYLTNPGNESAMYSEQLQDSLAREIVAGIMEYL 284 V RE+ MP LLEVG+L+N +ES ++ QD +A IV GI YL Sbjct: 412 VIRETKMPGALLEVGFLSNKTDESKLFDPATQDRVAEAIVRGIKNYL 458 >Q9LCR3.1 Q9LCR3_PAEPO CwlV Length = 499 Score = 187 bits (475), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 116/300 (38%), Positives = 161/300 (53%), Gaps = 25/300 (8%) Query: 1 MGSPDRIVIDFPGAVFAPDFAGSFPGVSTSGSPQGKLDVSGYPLVTEVRYALFSVSPPTA 60 M PDR+V+D P FA +F + G LD+ V+ +RYALF P T Sbjct: 206 MTGPDRVVVDLPATAFADNFGDQ---QKLDSNLNGSLDIENEADVSGIRYALFQKEPSTV 262 Query: 61 RFVIQTVGSQPYQVSKDDSTGLVTVDLNAVSSXXXX---------------XXXXXXXXK 105 R VI + Y + +D + V VDL + S K Sbjct: 263 RVVIDLKHAMNY-TAYNDGSNRVIVDLASKDSVNTTPDATLPDGSQPDDTQTSPVNSNGK 321 Query: 106 PVVVLDAGHGGTQSGAVSLTGK-LEKNFNLAVIQKAGALLMQAGLVDVIFTRTEDVTRGL 164 VVV+DAGHG SGAV ++ K EK FNLA+ K ++L Q ++V+ TR++D L Sbjct: 322 KVVVIDAGHGAKDSGAVGISRKNYEKTFNLAMALKVESILKQNPKLEVVLTRSDDTFLEL 381 Query: 165 QERVDIAERAKANLFISVHGNSLPADYPNRNKVNGSETYYSRAESLPLAQIMHKHLVAAT 224 ++RV +AE KAN+F+S+H NS + + NG+ETYY R+ S A +MHK+ AT Sbjct: 382 KQRVKVAENLKANVFVSIHANSSGS-----SASNGTETYYQRSASKAFANVMHKYFAPAT 436 Query: 225 GFKDNGIRSKSLHVTRESSMPAVLLEVGYLTNPGNESAMYSEQLQDSLAREIVAGIMEYL 284 G D GIR + HV RE++MPAVLLEVGYL+N E+ ++ E Q+ +A+ I GI EYL Sbjct: 437 GLTDRGIRYGNFHVIRETTMPAVLLEVGYLSNAKEEATLFDEDFQNRVAQGIADGITEYL 496 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,611,740,333 Number of extensions: 63040369 Number of successful extensions: 115876 Number of sequences better than 1.0e-05: 1782 Number of HSP's gapped: 114090 Number of HSP's successfully gapped: 1790 Length of query: 286 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 152 Effective length of database: 1,798,280,193 Effective search space: 273338589336 Effective search space used: 273338589336 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 130 (54.7 bits)