BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0007.p (479 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9LCR3.1 Q9LCR3_PAEPO CwlV 451 e-125 B3K708.1 B3K708_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.... 446 e-123 B3K710.1 B3K710_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.... 427 e-117 Q9LCR4.1 Q9LCR4_PAEPO CwlU 322 6e-86 B1DL77.1 B1DL77_9BACL N-acetylmuramoyl-L-alanine amidase EC=3.5.... 308 8e-82 >Q9LCR3.1 Q9LCR3_PAEPO CwlV Length = 499 Score = 451 bits (1161), Expect = e-125, Method: Compositional matrix adjust. Identities = 248/501 (49%), Positives = 318/501 (63%), Gaps = 29/501 (5%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKKF F+ PG GHAA +I+LD ++L+ + + S MVPLR+I+ Sbjct: 1 MKKFGFLLLLFVFMWAVPGYGHAASSGTQIYLDDQQLSVPSGAKAQVIGGSTMVPLRVIS 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 ENLGY V W Q+++T+TIE+ +I++I+ A+V+ + L +PLLRS+ LVP+RF Sbjct: 61 ENLGYDVTWKQQARTITIEKDSTSIRMIIGSKTATVNGSDISLDASPLLRSNYALVPLRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNLITPEIPDSTSNTGE----SSGDGGT---SVAVPPVDTTAN 173 IGEQ GL V WD+++K V L T S S GE SG+ T SV P + ++ Sbjct: 121 IGEQMGLDVKWDSSSKSVKLYTRS---SGSGNGEFTPPKSGNNSTGTGSVTAPTNNASSG 177 Query: 174 LTMVNGISFSENRLTIAMDGNSQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDLNG 233 +T V G+SFS+NRLTI +P +TGPDR+V+DLP F+D FG Q L +LNG Sbjct: 178 VTQVQGVSFSQNRLTITTASGVKPKAFTMTGPDRVVVDLPATAFADNFGDQQKLDSNLNG 237 Query: 234 SLAVTDYPDVSGVRYSLYSTNPYTVRFVIDLNNAKNYSVNVSGDVSKLVVVDL------- 286 SL + + DVSG+RY+L+ P TVR VIDL +A NY+ D S V+VDL Sbjct: 238 SLDIENEADVSGIRYALFQKEPSTVRVVIDLKHAMNYTA--YNDGSNRVIVDLASKDSVN 295 Query: 287 --------NAASTDDPATLPGN-NGRKLVVLDAGHGAKDSGAVGVTGK-YEKNFNXXXXX 336 + + DD T P N NG+K+VV+DAGHGAKDSGAVG++ K YEK FN Sbjct: 296 TTPDATLPDGSQPDDTQTSPVNSNGKKVVVIDAGHGAKDSGAVGISRKNYEKTFNLAMAL 355 Query: 337 XXXXXXXQENKVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXXXETY 396 Q K++VVLTRSDDTFLELK+R +A NLKA++F+S+HAN ETY Sbjct: 356 KVESILKQNPKLEVVLTRSDDTFLELKQRVKVAENLKANVFVSIHANSSGSSASNGTETY 415 Query: 397 YQRAESKALANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALL 456 YQR+ SKA ANVMHKY ATGL+DRG+RYGNFHVIRETTMPAVLLEVGYLSN +EA L Sbjct: 416 YQRSASKAFANVMHKYFAPATGLTDRGIRYGNFHVIRETTMPAVLLEVGYLSNAKEEATL 475 Query: 457 FSEDLQNKVAAGMVAGIKEYL 477 F ED QN+VA G+ GI EYL Sbjct: 476 FDEDFQNRVAQGIADGITEYL 496 >B3K708.1 B3K708_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 461 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 237/481 (49%), Positives = 314/481 (65%), Gaps = 23/481 (4%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKKF F+ P A+ S +I+LDG+++ V VEN N ++MVP+R+I+ Sbjct: 1 MKKFGFLMFLVVIMIAFPKMADASSYSARIYLDGQQINLSSGVKVENNNGTVMVPIRVIS 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 ENLG+ VNW++ +KTVT+E + + +++ A ++ + V++T AP+LR TTLVPIRF Sbjct: 61 ENLGFNVNWNKAAKTVTVEDAWQKVTMVIGSQYADINGQRVLMTKAPVLRGGTTLVPIRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNLITPEIPDSTSNTGESSGDGGTSVAVPPVDTTANLTMVNGI 180 + EQ GL+V WDNT K V L SS DG P V T+ V+G+ Sbjct: 121 VSEQMGLQVRWDNTQKAVYLT-------------SSYDGSNPGEEPGVATST----VDGL 163 Query: 181 SFSENRLTIAMDGNSQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDL--NGSLAVT 238 SFSENRL +A+ GN++P VSK++ PDRIVID+ N FS+ F + D+ +G + V+ Sbjct: 164 SFSENRLMVAVTGNAKPKVSKLSSPDRIVIDMANTNFSETFSSNADFD-DVAKSGQMTVS 222 Query: 239 DYPDVSGVRYSLYSTNPYTVRFVIDLNNAKNYSVNVSGDVSKLVVVDLNAASTDDPATLP 298 YPDV VRYSLY++NP TVR VIDLN AKNY+V+ + S L +VDLN +T +P+ P Sbjct: 223 GYPDVKAVRYSLYNSNPMTVRIVIDLNYAKNYTVSGLENGSGLFIVDLN--TTSNPSPEP 280 Query: 299 GNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDT 358 G G+KLVV+DAGHG D GA+GVT K EK+FN +E ++VVLTRS+DT Sbjct: 281 GTGGKKLVVIDAGHGDHDPGAIGVTKKREKDFNLAMALKVEKLLAKEKNIEVVLTRSNDT 340 Query: 359 FLELKERAGMANNLKADLFISVHANXXXXXXXXXXETYYQRAESKALANVMHKYLVQATG 418 FLELK+RA +AN+LKAD+FIS+HAN ETYY KALAN +HK +V+A G Sbjct: 341 FLELKDRAKIANDLKADVFISIHAN-SGPSTASGTETYYYNESDKALANALHKEIVKAMG 399 Query: 419 LSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYLK 478 L DRGVRYGNFHVIRET MP LLEVG+LSNK DE+ LF Q++VA +V GIK YLK Sbjct: 400 LKDRGVRYGNFHVIRETKMPGALLEVGFLSNKTDESKLFDPATQDRVAEAIVRGIKNYLK 459 Query: 479 L 479 + Sbjct: 460 V 460 >B3K710.1 B3K710_9BACI N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 478 Score = 427 bits (1097), Expect = e-117, Method: Compositional matrix adjust. Identities = 218/481 (45%), Positives = 304/481 (63%), Gaps = 6/481 (1%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKK F+ P G A+ S IFLDG+ L V V N+ ++M+P+R+++ Sbjct: 1 MKKLGFVMVLIVFLLAFPKSGEASSSSATIFLDGEPLNLSSGVQVANMKGNVMIPIRVVS 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 ENLG++V W++ S+TVT+E KT++++V A ++ V L AP+L TTLVP+RF Sbjct: 61 ENLGFKVGWEKASQTVTVENSEKTVRMVVGSQQAELNGAQVDLNLAPVLNGPTTLVPLRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNLITPEIPDSTSNTGESSGDGGTSVAVPPVDTTANLTMVNGI 180 + EQ GL V WDN +K V L + +P + + G + PPV+T N + + G+ Sbjct: 121 VSEQMGLDVKWDNQSKAVYLSSAALPTTGAEIDPGEGSNPGNQTPPPVET-GNKSTLTGL 179 Query: 181 SFSENRLTIAMDGNSQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDLNGSLAVTDY 240 SFS+NRL IA +G P V +TGP RIVIDLP+ +S F +G ++ GS+ +Y Sbjct: 180 SFSDNRLMIATEGAVTPKVFSMTGPARIVIDLPDTVYSASFLSGHAVNAQNGGSMEAANY 239 Query: 241 PDVSGVRYSLYSTNPYTVRFVIDLNNAKNYSVNVSGDVSKLVVVDLNAASTDDPATLP-- 298 DV+ +RY+++S NP TVR V+DL KNY+V S +++VDLNA +P Sbjct: 240 SDVTNIRYAVFSDNPPTVRIVLDLTGPKNYAVTQE---SGIIIVDLNAEGEILVPPVPPV 296 Query: 299 GNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDT 358 NG+K VV+D GHG G++ +TGK EK+FN +E ++VV+TRSDD+ Sbjct: 297 TGNGKKTVVIDPGHGGIKPGSISLTGKTEKSFNLDISLKVEALLKKEKNINVVMTRSDDS 356 Query: 359 FLELKERAGMANNLKADLFISVHANXXXXXXXXXXETYYQRAESKALANVMHKYLVQATG 418 LEL +R +ANNLKAD+F+S+H N ETYY R SK+LA+V+HK+L +ATG Sbjct: 357 HLELSDRVKIANNLKADIFVSIHGNSNASSSPSGTETYYTRDSSKSLADVIHKHLAKATG 416 Query: 419 LSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYLK 478 L DRGV+YG+ HV RETTMPAVLLE+G+LSNKGDEA LF +D QN+VA G+V GIKEYLK Sbjct: 417 LKDRGVKYGSLHVTRETTMPAVLLEIGFLSNKGDEAQLFKDDFQNRVAQGIVEGIKEYLK 476 Query: 479 L 479 + Sbjct: 477 V 477 >Q9LCR4.1 Q9LCR4_PAEPO CwlU Length = 524 Score = 322 bits (824), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 192/518 (37%), Positives = 274/518 (52%), Gaps = 46/518 (8%) Query: 1 MKKFAFMXXXXXXXXXXPGQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIA 60 MKK+ ++ P H ++FLDGK + A D E VN +MVPLR++ Sbjct: 1 MKKYGWVILAAILIWLWPSSPHVNAAGAELFLDGKRVEAPADAKPEMVNGKVMVPLRVVG 60 Query: 61 ENLGYQVNWDQKSKTVTIEQQGKTIKLIVDQTAASVDDKTVVLTTAPLLRSDTTLVPIRF 120 E LGYQ W+ ++ ++I++ + + V +T+A V+ KTV L P+LR +T++P+RF Sbjct: 61 EQLGYQFKWEPQAYKISIQKNSTDMSMYVGRTSADVNGKTVSLDAPPVLRGSSTMIPLRF 120 Query: 121 IGEQFGLKVTWDNTTKVVNL---ITPEIPD-----------------STSNTGESS---- 156 +GEQ GLKV W+N K VNL I + D ST+ T ++ Sbjct: 121 VGEQMGLKVDWNNQNKSVNLSQKIATQPADKQSQSQQPSQTSTQSPKSTNKTSTATATTI 180 Query: 157 ----GDGGTSVAVPPV-------DTTANLTMVNGISFSENRLTIAMDGNSQPVVSKVTGP 205 D T V VP TTAN +V ISF L I+++ + P VSK+TGP Sbjct: 181 NPVEADKHTEV-VPSTSKEQNVEQTTANAVLVQNISFESETLKISLNKDLTPKVSKMTGP 239 Query: 206 DRIVIDLPNATFSDVFGTGQVLSPDLNGSLAVTDYPDVSGVRYSLYSTNPYTVRFVIDLN 265 DRIV+DLP A+ S + D +L ++D DV +R++ R VI LN Sbjct: 240 DRIVVDLPEASLSADLLQSFPVRKDGTRALTISDSEDVQEIRFAPADGTKGGARIVIALN 299 Query: 266 NAKNY--SVNVSGDVSKLVVVDLNAASTDDPATLPGN-NGRKLVVLDAGHGAKDSGAVGV 322 ++Y S N GD++ + L S + P GR++VV+D GHG KD GA V Sbjct: 300 QTRDYDLSTNGVGDIT----LQLRERSVNPEIVAPTYVEGRRIVVIDPGHGGKDPGAGSV 355 Query: 323 TGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDTFLELKERAGMANNLKADLFISVHA 382 TG++EK F + + +VLTR+ DT+ L ER +ANN +A +F+S+H Sbjct: 356 TGRHEKEFTLAVALKVQQLAQNDPDIQIVLTRNGDTYPTLDERPQLANNQQASVFVSIHG 415 Query: 383 NXXXXX---XXXXXETYYQRAESKALANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPA 439 N ETYY R ES +LA +MHK+LV AT D G++ N V R++ MPA Sbjct: 416 NSMPTSNNGKANGSETYYARQESLSLATIMHKHLVAATEFKDNGIKVANHVVTRKSQMPA 475 Query: 440 VLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYL 477 VLLE GYLSN +EA +F+E+ Q K+A G+V G+KEYL Sbjct: 476 VLLECGYLSNPSNEAAMFTEETQQKIAEGIVDGLKEYL 513 >B1DL77.1 B1DL77_9BACL N-acetylmuramoyl-L-alanine amidase EC=3.5.1.28 Length = 473 Score = 308 bits (789), Expect = 8e-82, Method: Compositional matrix adjust. Identities = 194/476 (40%), Positives = 267/476 (56%), Gaps = 41/476 (8%) Query: 19 GQGHAAGGSNKIFLDGKELTAGLDVPVENVNNSIMVPLRMIAENLGYQVNWDQKSKTVTI 78 GQ K++L+ +L D P + VNN +VP+R+++EN+GY+VNW +KTVTI Sbjct: 23 GQAATTPVVPKLYLN-DQLLKPYDDP-QIVNNFTLVPIRVVSENMGYEVNWAAATKTVTI 80 Query: 79 EQQGKTIKLIVDQTAASVDDKTVVL-TTAPLLRSDTTLVPIRFIGEQFGLKVTWDNTTKV 137 I L +D A V++ V + +A L + TT++PIRF+ EQ GL V WD ++ Sbjct: 81 HNDQNEIVLTIDNNIALVNNNKVQMDMSAKLQKGRTTMIPIRFVSEQLGLTVKWDQASQS 140 Query: 138 VNLIT---PEIPDSTSNTGESSGDGGTSVAVPPVDTTANLTMVN-GISFSENRLTIAMDG 193 V L PE P +T + G+ T ++T V SF + I DG Sbjct: 141 VYLYKAADPEDPSTTPDPGQ----------------TGSITSVQYDSSFG---IIINYDG 181 Query: 194 N---SQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDLNGSLAVTDYPDVSGVRYSL 250 ++P K+ P RIVID PN T+SD + Q + + + V D P +S RYS+ Sbjct: 182 KVTANKPF--KLDSPKRIVIDFPNTTYSDNLSS-QFMGAETR--IPVADNPYISMFRYSV 236 Query: 251 YSTNPYTVRFVIDLNNAKNYSVNVSGDVSKLVVVDLNAASTDDPATLPGNN-----GRKL 305 +STNP T R V+DLN K+ V + + ++ + L T P G K+ Sbjct: 237 FSTNPATARLVLDLNTDKDADAVVQDNGAGVITIGLTDPVTTPPVDTTTPPVTTDPGTKV 296 Query: 306 --VVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDTFLELK 363 VV+DAGHG D GA + G++EK N +E + +L+R +D F+ L Sbjct: 297 YNVVIDAGHGGTDPGAQSINGRWEKEVNLAVSLKVKALLDKEKNIKPLLSRPEDKFVTLA 356 Query: 364 ERAGMANNLKADLFISVHANXXXXXXXXXXETYYQRAESKALANVMHKYLVQATGLSDRG 423 +R A N KAD+FIS+HAN ETYY R SKALANV+HK+LV+ATGL DRG Sbjct: 357 DRVTFAKNNKADIFISIHANSNPTSSVTGTETYYTRDSSKALANVIHKHLVKATGLKDRG 416 Query: 424 VRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYLKL 479 V+YGN HV RETTMPA+LLE G+LSNKGD +L+S+ QNK+AA +VAGIKEYLKL Sbjct: 417 VKYGNLHVTRETTMPAILLETGFLSNKGDSDILYSDAAQNKMAAEIVAGIKEYLKL 472 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.313 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,640,228,612 Number of extensions: 108353163 Number of successful extensions: 229687 Number of sequences better than 1.0e-05: 1922 Number of HSP's gapped: 227269 Number of HSP's successfully gapped: 1943 Length of query: 479 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 339 Effective length of database: 1,741,754,103 Effective search space: 590454640917 Effective search space used: 590454640917 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)