BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0002.p (223 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K705.1 B3K705_9BACI Endonuclease III EC=4.2.99.18 409 e-112 Q9LCR1.1 Q9LCR1_PAEPO End3 391 e-107 B1DL74.1 B1DL74_9BACL Endonuclease III EC=4.2.99.18 352 1e-95 C1PF30.1 C1PF30_BACCO Endonuclease III EC=4.2.99.18 343 8e-93 B7GHU0.1 B7GHU0_ANOFW Predicted EndoIII-related endonuclease 338 2e-91 >B3K705.1 B3K705_9BACI Endonuclease III EC=4.2.99.18 Length = 223 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 192/222 (86%), Positives = 204/222 (91%) Query: 1 MKAAEVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKT 60 M AA VRHILDT+ +FPDAHCELNHSNAFELTIAVLLSAQCTD TVNKVT+DLFQKYKT Sbjct: 1 MNAATVRHILDTMESMFPDAHCELNHSNAFELTIAVLLSAQCTDETVNKVTKDLFQKYKT 60 Query: 61 PLDYISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRK 120 PLDY+SVPIEELE DIRRIGLYRNKAKHIQ+LC ILIEQYGGEVP+AHD LV LPGVGRK Sbjct: 61 PLDYVSVPIEELEQDIRRIGLYRNKAKHIQNLCRILIEQYGGEVPEAHDELVKLPGVGRK 120 Query: 121 TANVVVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRL 180 TANVVVSNAFGVPAIAVDTHVERV+KRL LA WKDSVLEVEKKLMK VPR+EWT+THHR+ Sbjct: 121 TANVVVSNAFGVPAIAVDTHVERVSKRLGLAAWKDSVLEVEKKLMKRVPREEWTMTHHRI 180 Query: 181 IFFGRYHCKAQNPKCQVCPLLDVCREGKKRMKTAVIRKAKSH 222 IFFGRYHCKAQNP+C VCPLLDVCREGKKRMKT +RK K Sbjct: 181 IFFGRYHCKAQNPQCPVCPLLDVCREGKKRMKTLAVRKNKEQ 222 >Q9LCR1.1 Q9LCR1_PAEPO End3 Length = 224 Score = 391 bits (1004), Expect = e-107, Method: Compositional matrix adjust. Identities = 182/220 (82%), Positives = 197/220 (89%) Query: 1 MKAAEVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKT 60 M AA RHILDTIG +FPDAHCELNH NAFELTIAVLLSAQC+D VNKVT DLFQKYK+ Sbjct: 1 MNAATARHILDTIGTMFPDAHCELNHDNAFELTIAVLLSAQCSDQMVNKVTADLFQKYKS 60 Query: 61 PLDYISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRK 120 P DY++VP+EELE DIRRIGLYRNKAKHI +LC ILI+QYGGE+P HD LV LPGVGRK Sbjct: 61 PEDYLAVPLEELEQDIRRIGLYRNKAKHIHNLCRILIDQYGGEIPSEHDQLVKLPGVGRK 120 Query: 121 TANVVVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRL 180 TANVVVS AF VPAIAVDTHVERV+KRL AGW DSVLEVEKKLMK VPRDEW++THHRL Sbjct: 121 TANVVVSTAFDVPAIAVDTHVERVSKRLGFAGWDDSVLEVEKKLMKRVPRDEWSVTHHRL 180 Query: 181 IFFGRYHCKAQNPKCQVCPLLDVCREGKKRMKTAVIRKAK 220 IFFGRYHCKAQNP+CQVCPLLDVCREGKKRMKT++IRK K Sbjct: 181 IFFGRYHCKAQNPQCQVCPLLDVCREGKKRMKTSLIRKDK 220 >B1DL74.1 B1DL74_9BACL Endonuclease III EC=4.2.99.18 Length = 233 Score = 352 bits (904), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 163/216 (75%), Positives = 189/216 (87%) Query: 5 EVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPLDY 64 ++RHILDT+ ++FPDAHCEL+HSN FELTIAVLLSAQCTD TVNKVT +LFQKYK P DY Sbjct: 6 KMRHILDTLAEMFPDAHCELHHSNPFELTIAVLLSAQCTDETVNKVTVNLFQKYKRPEDY 65 Query: 65 ISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTANV 124 ++VP+EELE DIRRIGL+R+KA +IQ LC ILI++Y GEVP+ H+ L LPGVGRKTANV Sbjct: 66 LAVPLEELEQDIRRIGLFRSKASNIQKLCRILIDKYEGEVPERHEQLTELPGVGRKTANV 125 Query: 125 VVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLIFFG 184 VVSNAFGVPAIAVDTHVERV+KRL +A D+VLEVEKKLMK VPR+EWTLTHHRLIFFG Sbjct: 126 VVSNAFGVPAIAVDTHVERVSKRLGMAKLDDTVLEVEKKLMKLVPREEWTLTHHRLIFFG 185 Query: 185 RYHCKAQNPKCQVCPLLDVCREGKKRMKTAVIRKAK 220 RYHCKAQNP+C +CPLLD+C+EGK+RMK K K Sbjct: 186 RYHCKAQNPQCPICPLLDMCKEGKQRMKPGGQTKTK 221 >C1PF30.1 C1PF30_BACCO Endonuclease III EC=4.2.99.18 Length = 219 Score = 343 bits (879), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 154/208 (74%), Positives = 184/208 (88%) Query: 5 EVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPLDY 64 ++RH L+T+G++FPDA CELNHSN FEL IAV LSAQCTDA VNKVT++LFQKYKTP DY Sbjct: 6 QIRHCLETMGEMFPDARCELNHSNPFELLIAVTLSAQCTDALVNKVTKNLFQKYKTPDDY 65 Query: 65 ISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTANV 124 I+VP+EEL+ DIR IGLYRNKAK+IQ LC +LIE+YG EVPQ + L+ LPGVGRKTANV Sbjct: 66 IAVPLEELQQDIRSIGLYRNKAKNIQKLCRMLIEEYGREVPQTREELMKLPGVGRKTANV 125 Query: 125 VVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLIFFG 184 V+S AFG+PAIAVDTHVERV+KRL +KDSVLEVE+ LMK VP++EW++THHR+IFFG Sbjct: 126 VLSVAFGIPAIAVDTHVERVSKRLGFCRYKDSVLEVEQTLMKKVPKEEWSITHHRMIFFG 185 Query: 185 RYHCKAQNPKCQVCPLLDVCREGKKRMK 212 RYHCKAQ P+C++CPLLD+CREGKKRMK Sbjct: 186 RYHCKAQRPQCEICPLLDLCREGKKRMK 213 >B7GHU0.1 B7GHU0_ANOFW Predicted EndoIII-related endonuclease Length = 225 Score = 338 bits (868), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 156/208 (75%), Positives = 181/208 (87%) Query: 5 EVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPLDY 64 ++R+ LD I ++FP+AHCEL H N FEL IAV+LSAQCTDA VNKVT+ LF+KYKTP DY Sbjct: 15 QIRYCLDEIANMFPNAHCELVHRNPFELLIAVVLSAQCTDALVNKVTKQLFEKYKTPEDY 74 Query: 65 ISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTANV 124 +SVP+EEL+ DIR IGLYRNKAK+IQ LC ILIEQY GEVP+ D L+ LPGVGRKTANV Sbjct: 75 VSVPLEELQQDIRSIGLYRNKAKNIQQLCRILIEQYSGEVPKNRDELMKLPGVGRKTANV 134 Query: 125 VVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLIFFG 184 VVS AFGVPAIAVDTHVERV+KRL + WKDSVLEVE+ LMK +P++EW++THHRLIFFG Sbjct: 135 VVSVAFGVPAIAVDTHVERVSKRLGICRWKDSVLEVEETLMKKIPKEEWSVTHHRLIFFG 194 Query: 185 RYHCKAQNPKCQVCPLLDVCREGKKRMK 212 RYHCKAQ+PKC VCPLL +CREGKKRMK Sbjct: 195 RYHCKAQSPKCDVCPLLHLCREGKKRMK 222 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,249,910,027 Number of extensions: 48553107 Number of successful extensions: 163124 Number of sequences better than 1.0e-05: 2848 Number of HSP's gapped: 160701 Number of HSP's successfully gapped: 2848 Length of query: 223 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 92 Effective length of database: 1,826,543,238 Effective search space: 168041977896 Effective search space used: 168041977896 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)