BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0016.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAT7.1 B1DAT7_9BACL Binding-protein-dependent transport system... 444 e-123 B9MLN8.1 B9MLN8_ANATD Binding-protein-dependent transport system... 238 4e-61 A4XGB0.1 A4XGB0_CALS8 Binding-protein-dependent transport system... 234 5e-60 B9MP61.1 B9MP61_ANATD Binding-protein-dependent transport system... 234 7e-60 A4XMJ1.1 A4XMJ1_CALS8 Binding-protein-dependent transport system... 231 6e-59 >B1DAT7.1 B1DAT7_9BACL Binding-protein-dependent transport systems inner membrane component Length = 293 Score = 444 bits (1142), Expect = e-123, Method: Compositional matrix adjust. Identities = 211/290 (72%), Positives = 240/290 (82%) Query: 4 SQRQKDLLLDSIIYTVLFLIMLTMLYPFYYVLIASFNKGSDSLLGGVYLWPRSFTLENYK 63 SQ+QKD +D ++Y +L ++ L+MLYPFYYVLI SFNKG+DSLLGGVYLWPR+FT ENY Sbjct: 4 SQKQKDRFMDVLVYALLAILTLSMLYPFYYVLILSFNKGTDSLLGGVYLWPRTFTFENYA 63 Query: 64 MFLDDPKWYRAFIVTVVRXXXXXXXXXXXXXXXXXXXXHRDLLFSKLYFAIIIFAMYFSG 123 FLDDP WYRAF+VT++R HRDLLF K YF +IIFAMYFSG Sbjct: 64 RFLDDPHWYRAFLVTIMRTLAGTALGVLFTCLVAYGLSHRDLLFRKSYFTVIIFAMYFSG 123 Query: 124 GLIPYYVVLRSIGLLNSFGVYIIPSMLSTFFLLIAISFFREIPGELKESAHIDGAGEFVI 183 GLIPYYVVLRS+GLLN+FGVY+IPSMLSTFFLLIAISFFREIP ELKESAH+DGAGE VI Sbjct: 124 GLIPYYVVLRSVGLLNTFGVYVIPSMLSTFFLLIAISFFREIPSELKESAHMDGAGELVI 183 Query: 184 FFRIILPVSMPLLATMALFLGVGQWNSWLDSAYFVQSENLRTLAFRMMEVINKSNSPMDX 243 F+RI+LPVSMPL+ATM+LFLGVGQWNSWLDSAYFVQSE LRTLAFRMMEVINKSN+PMD Sbjct: 184 FYRIMLPVSMPLIATMSLFLGVGQWNSWLDSAYFVQSEGLRTLAFRMMEVINKSNAPMDA 243 Query: 244 XXXXXXXXXGVTSFSLQVTAMVVSIVPIICVYPFLQKYFVHGIMLGSVKG 293 GVTS+SLQV AMV+SI PI+CVYPFLQ++F+ GIMLGSVKG Sbjct: 244 IAVANSASAGVTSYSLQVAAMVISIAPIVCVYPFLQRFFIQGIMLGSVKG 293 >B9MLN8.1 B9MLN8_ANATD Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 238 bits (607), Expect = 4e-61, Method: Compositional matrix adjust. Identities = 119/286 (41%), Positives = 182/286 (63%), Gaps = 1/286 (0%) Query: 9 DLLLDSIIYTVLFLIMLTMLYPFYYVLIASFNKGSDSLLGGVYLWPRSFTLENYKMFLDD 68 D + D T++ +I LYP +Y+++ SFN+G D++LGG+Y WPR FTL+NYK + Sbjct: 9 DKVFDIFNITLMLIICFLTLYPIWYIIVYSFNEGKDAMLGGIYFWPRKFTLDNYKTVFSN 68 Query: 69 PKWYRAFIVTVVRXXXXXXXXXXXXXXXXXXXXHRDLLFSKLYFAIIIFAMYFSGGLIPY 128 AF+VTV R ++LL K+Y A+ ++F GGLIPY Sbjct: 69 SDITTAFMVTVARTVITTTLHVFFTAMVAYAFLRKELLGRKIYMAMGTITLFFGGGLIPY 128 Query: 129 YVVLRSIGLLNSFGVYIIPSMLSTFFLLIAISFFREIPGELKESAHIDGAGEFVIFFRII 188 +++++S+GL N+F VY+IP M + + L+I +FFRE+P EL+ESA IDGA +F+IF RII Sbjct: 129 FLLIKSLGLYNTFWVYVIPGMFNFYNLIIFQAFFRELPIELEESAKIDGANDFIIFTRII 188 Query: 189 LPVSMPLLATMALFLGVGQWNSWLDSAYFVQSENLRTLAFRMMEVINK-SNSPMDXXXXX 247 LP+S P+LAT+ALF+GV WN + F+ + L+ + + +VI + +++ M Sbjct: 189 LPLSTPVLATIALFVGVYNWNDYFMGVIFINNPKLQPIQTFLYKVIAQVTSNQMLAYAPG 248 Query: 248 XXXXXGVTSFSLQVTAMVVSIVPIICVYPFLQKYFVHGIMLGSVKG 293 VTS SL++ MV++ +PI+CVYPFLQKYFV G+++G++KG Sbjct: 249 GIATRNVTSQSLKMATMVITTLPIVCVYPFLQKYFVKGLLIGAIKG 294 >A4XGB0.1 A4XGB0_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 234 bits (598), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 113/294 (38%), Positives = 183/294 (62%), Gaps = 4/294 (1%) Query: 3 KSQRQKDLLLDSIIYTVLFLIMLTMLYPFYYVLIASFNKGSDSLLGGVYLWPRSFTLENY 62 K + +DL++D ++YTV+ ++M+ LYPF+ +L S N DS+ GG+YLWPR FTL NY Sbjct: 6 KYRTTEDLIIDIVVYTVMIIVMIATLYPFWNILAISLNDALDSIRGGIYLWPRKFTLNNY 65 Query: 63 KMFLDDPKWYRAFIVTVVRXXXXXXXXXXXXXXXXXXXXHRDLLFSKLYFAIIIFAMYFS 122 ++ L +P Y A +++V+R +D +F K +I+F MYFS Sbjct: 66 RVILSNPDIYHATLISVLRAVIGSITNVLSCLMVAYGISRKDYIFRKFISRVIVFTMYFS 125 Query: 123 GGLIPYYVVLRSIGLLNSFGVYIIPSMLSTFFLLIAISFFREIPGELKESAHIDGAGEFV 182 GGLIP Y++++++ L+ +F VYI+P M+S F +++ S+ +P L ESA IDGA E+ Sbjct: 126 GGLIPTYLLMKNLHLVGTFWVYILPGMVSAFNIIVIRSYIDGLPQSLIESAKIDGASEYR 185 Query: 183 IFFRIILPVSMPLLATMALFLGVGQWNSWLDS-AYFVQSENLRTLAF---RMMEVINKSN 238 I F+II+P+ +P+LAT+ L++ VGQWN+W D+ Y L TL F ++++ + ++ Sbjct: 186 ILFQIIMPLCLPVLATVTLWVAVGQWNAWFDTFLYNSGKPELSTLQFELQKILQSVQSAS 245 Query: 239 SPMDXXXXXXXXXXGVTSFSLQVTAMVVSIVPIICVYPFLQKYFVHGIMLGSVK 292 + D VT +++ T +V+ VPI+ VYPFLQ+YF+HG+ +GS+K Sbjct: 246 TNPDFSASLTSSGRTVTPTAIRATMTIVATVPILFVYPFLQRYFIHGLTIGSIK 299 >B9MP61.1 B9MP61_ANATD Binding-protein-dependent transport systems inner membrane component Length = 300 Score = 234 bits (597), Expect = 7e-60, Method: Compositional matrix adjust. Identities = 113/294 (38%), Positives = 183/294 (62%), Gaps = 4/294 (1%) Query: 3 KSQRQKDLLLDSIIYTVLFLIMLTMLYPFYYVLIASFNKGSDSLLGGVYLWPRSFTLENY 62 K + +DL++D ++YTV+ ++M+ LYPF+ +L S N DS+ GG+YLWPR FTL NY Sbjct: 6 KYRTTEDLVIDIVVYTVMIIVMIATLYPFWNILAISLNDALDSIRGGIYLWPRKFTLNNY 65 Query: 63 KMFLDDPKWYRAFIVTVVRXXXXXXXXXXXXXXXXXXXXHRDLLFSKLYFAIIIFAMYFS 122 ++ L +P Y A +++V+R +D +F K +I+F MYFS Sbjct: 66 RVILSNPDIYHATLISVLRAVIGSLTNVLSCLMVAYGISRKDYIFRKFISRVIVFTMYFS 125 Query: 123 GGLIPYYVVLRSIGLLNSFGVYIIPSMLSTFFLLIAISFFREIPGELKESAHIDGAGEFV 182 GGLIP Y++++++ L+ +F VYI+P M+S F +++ S+ +P L ESA IDGA E+ Sbjct: 126 GGLIPTYLLMKNLHLVGTFWVYILPGMVSAFNIIVIRSYIDGLPQSLIESAKIDGASEYR 185 Query: 183 IFFRIILPVSMPLLATMALFLGVGQWNSWLDS-AYFVQSENLRTLAF---RMMEVINKSN 238 I F+II+P+ +P+LAT+ L++ VGQWNSW D+ Y L TL F ++++ + ++ Sbjct: 186 ILFQIIIPLCLPVLATVTLWVAVGQWNSWFDTFLYNSGKPELSTLQFELQKILQSVQSAS 245 Query: 239 SPMDXXXXXXXXXXGVTSFSLQVTAMVVSIVPIICVYPFLQKYFVHGIMLGSVK 292 + D VT +++ T +V+ +PI+ VYPFLQ+YF+HG+ +GS+K Sbjct: 246 TNPDFSASLTSSGRTVTPTAIRATMTIVATLPILFVYPFLQRYFIHGLTIGSIK 299 >A4XMJ1.1 A4XMJ1_CALS8 Binding-protein-dependent transport systems inner membrane component Length = 294 Score = 231 bits (589), Expect = 6e-59, Method: Compositional matrix adjust. Identities = 115/286 (40%), Positives = 177/286 (61%), Gaps = 1/286 (0%) Query: 9 DLLLDSIIYTVLFLIMLTMLYPFYYVLIASFNKGSDSLLGGVYLWPRSFTLENYKMFLDD 68 D + D T++ +I LYP +Y+++ SFN G D++LGG+Y WPR FTL+NY + Sbjct: 9 DKIFDIFNITLMLIICFVTLYPIWYIIVYSFNDGKDAMLGGIYFWPRKFTLDNYITVFSN 68 Query: 69 PKWYRAFIVTVVRXXXXXXXXXXXXXXXXXXXXHRDLLFSKLYFAIIIFAMYFSGGLIPY 128 AF+VT+ R ++L+ K+Y + ++F GGLIPY Sbjct: 69 SDITTAFMVTIARTVITTTLHVFFTAMVAYAFMKKELIGRKIYMTMGTITLFFGGGLIPY 128 Query: 129 YVVLRSIGLLNSFGVYIIPSMLSTFFLLIAISFFREIPGELKESAHIDGAGEFVIFFRII 188 +++++S+GL N+F VY+IP M + + L+I +FFRE+P EL+ESA IDGA +F IF R+I Sbjct: 129 FLLIKSLGLYNNFLVYVIPGMFNFYNLIIFQAFFRELPVELEESAKIDGANDFTIFTRVI 188 Query: 189 LPVSMPLLATMALFLGVGQWNSWLDSAYFVQSENLRTLAFRMMEVINKSNS-PMDXXXXX 247 LP+S P+LAT+ALF+GV WN + F+ + L+ + + +VI ++ S M Sbjct: 189 LPLSTPVLATIALFVGVYNWNDYFMGVIFINNPKLQPIQTFLYKVIAETTSNQMLANAPG 248 Query: 248 XXXXXGVTSFSLQVTAMVVSIVPIICVYPFLQKYFVHGIMLGSVKG 293 VTS S+++ MVV+ +PI+CVYPFLQKYFV G+++G++KG Sbjct: 249 GIMTRNVTSQSIKMATMVVTTLPIVCVYPFLQKYFVKGLLIGAIKG 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.331 0.146 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,440,789,757 Number of extensions: 51031193 Number of successful extensions: 207067 Number of sequences better than 1.0e-05: 5099 Number of HSP's gapped: 200551 Number of HSP's successfully gapped: 5099 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 130 (54.7 bits)