BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0015.p (537 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DAT8.1 B1DAT8_9BACL Extracellular solute-binding protein family 1 848 0.0 B3KH15.1 B3KH15_9BACI Extracellular solute-binding protein family 1 159 8e-37 A9U612.1 A9U612_PHYPA Predicted protein 156 6e-36 B1DL52.1 B1DL52_9BACL Extracellular solute-binding protein family 1 134 3e-29 B1D8P2.1 B1D8P2_9BACL Extracellular solute-binding protein family 1 131 2e-28 >B1DAT8.1 B1DAT8_9BACL Extracellular solute-binding protein family 1 Length = 530 Score = 848 bits (2192), Expect = 0.0, Method: Compositional matrix adjust. Identities = 415/531 (78%), Positives = 458/531 (86%), Gaps = 12/531 (2%) Query: 8 RMLTAVLAAIMMISLLSACXXXXXXXXXXXKPDNAAQGDNAGSKD--NTGAAGNSGNGEV 65 + L L +M I+L+SAC +N Q +NAG+ + T + + N EV Sbjct: 8 KALLLSLILMMTIALMSACGGNN---------NNDGQKENAGTANPSETSSEPPAKNNEV 58 Query: 66 KELSLFIDAPWYPVKEWKGPVADKITEKTGVKLKVTVATDDKQLPLMIASGDLPDLVFTY 125 KELSLFIDA WYPVKEWKG VA+KITEKTG+KLKVTVATDDKQLPLMIASGDLPDLVFTY Sbjct: 59 KELSLFIDASWYPVKEWKGSVAEKITEKTGIKLKVTVATDDKQLPLMIASGDLPDLVFTY 118 Query: 126 SNVGRMSDSKLSYPWNELIEKYAPDFKIDKTRIAIHTMDDGNFYTVRNSFATQEEMKQNK 185 +N+ RMSDSKLSYPWNELIEKYAPDFKID TRIAIHTMDDGNFYTVRNSFATQEEM QNK Sbjct: 119 ANIDRMSDSKLSYPWNELIEKYAPDFKIDPTRIAIHTMDDGNFYTVRNSFATQEEMAQNK 178 Query: 186 YSIGSDGNPGIAVREDILKELGNPPINSLDDFVKVLGMVKKKYPDMVPLIMDKDWLEQYF 245 YS+GSDGNPGIAVREDILKELGNPPI+++D+FVKVLGMVK+KYPDMVPLIMDKDW+EQYF Sbjct: 179 YSLGSDGNPGIAVREDILKELGNPPIDTIDNFVKVLGMVKQKYPDMVPLIMDKDWIEQYF 238 Query: 246 LQQFGTGVLLDGWYEQDGKVDYSIRQPKMLEFFKFMNSLYRNGYILAENFALANDQIDDQ 305 LQQFG LLDGWYE+DGKVDY+IRQP+MLEFFKFMN LYR G ILAENFA NDQIDDQ Sbjct: 239 LQQFGVEGLLDGWYERDGKVDYAIRQPEMLEFFKFMNRLYREGDILAENFAYTNDQIDDQ 298 Query: 306 YATNGKAFAHSHTVATADTDNXXXXXXXXXXYKFKMLPSALTKDAKVVSAGLGFAGTFIT 365 YAT+GKAFAHSHTV+ AD DN YKF+MLPSA++K+AK VS+GLGFAGTFIT Sbjct: 299 YATSGKAFAHSHTVSIADMDN-IKVKNAGGSYKFQMLPSAISKNAKAVSSGLGFAGTFIT 357 Query: 366 KKNKDPETSIKFLQYLASDEGKKLTMFGVEGEHWTWNNEGHPDLKYNPSDADFVNGNGIK 425 KKNKDPE SIKFLQYLASDEGKKLTMFGVEGEHWTWN+EG+P+LKY+PSDADFVN NGIK Sbjct: 358 KKNKDPEASIKFLQYLASDEGKKLTMFGVEGEHWTWNDEGYPNLKYDPSDADFVNSNGIK 417 Query: 426 WWYLYNDGVTEGMLSYVPGLQKTQALMETKAITVYKPQIGLIQAQPDSEEKTIKTKIDEM 485 WWYLYNDGVTEGMLSYVPG QKT+ALME K TVYKP++GLIQ QPDSEEKTIKTKIDEM Sbjct: 418 WWYLYNDGVTEGMLSYVPGAQKTKALMEKKEFTVYKPELGLIQLQPDSEEKTIKTKIDEM 477 Query: 486 VKNEKVRIYLANSEEEAVANYNSMVKKAEDMGLQKLVDWANATYNKKKDLF 536 +KNEKV+IYLA SEEEAVANY +MVK AE +GL +LVDWAN TY KKKDLF Sbjct: 478 IKNEKVKIYLAKSEEEAVANYETMVKNAEKIGLDRLVDWANQTYQKKKDLF 528 >B3KH15.1 B3KH15_9BACI Extracellular solute-binding protein family 1 Length = 537 Score = 159 bits (401), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 155/578 (26%), Positives = 264/578 (45%), Gaps = 93/578 (16%) Query: 8 RMLTAVLAAIMMISLLSACXXXXXXXXXXXKPDNAAQGDNAGSKDNTGAAGNSGNGEVKE 67 + L +LAA ++++ LSAC AA G ++G++ ++ A Sbjct: 2 KKLKLILAAALVMTTLSACGGN----------QKAASGGDSGTETDSNAP---------- 41 Query: 68 LSLFIDAPWYPVK---EWKGPVADKITEKTGVKLKVTVATDD--KQLPLMIASGDLPDLV 122 I W+ + PVA ITEKTGV +++ T + ++L LM+ASGDLPD++ Sbjct: 42 ----ITLTWFDQNTGDAFTNPVAKVITEKTGVTVEIQQPTGNPVEKLNLMLASGDLPDVI 97 Query: 123 FTYSNVGRMSD---SKLSYPWNELIEKYAPDF-KIDKTRIAIHTMDDGNFYTVRNSFATQ 178 M+ S P N+LIE++ P+ K+ +A +DGN Y N + + Sbjct: 98 LMSRGSDIMNKYITSGAVIPLNDLIEEHGPNIQKMYGDTLAKTRSEDGNNYYFSNWYGLE 157 Query: 179 EEMKQNKYSIGSDGNPGIAVREDILKELG------NPPINSLDDFVKVLGMVKKKYPDM- 231 + Y + G +R DI+KELG N + ++F ++L K+KYP + Sbjct: 158 Q------YPVF-----GFMMRMDIMKELGVGDKVNNGEPFTAEEFEQLLVKFKEKYPTID 206 Query: 232 ----VPLIMDKD---WLEQYFLQQFGTGVLLDGWYEQDGKVDYSIRQPKMLEFFKFMNSL 284 +P+ ++ + + F +G + +YE +G++ +R P+ LE KF+NSL Sbjct: 207 GKESIPMTLNGENVGAVTGSFKGMYG----MMPYYETNGELKKDVRDPRYLEMVKFLNSL 262 Query: 285 YRNGYILAENFALANDQIDDQYATNGKAFAHSHTVATADTDNXXXXXXXX--------XX 336 YR G + E + Q + Q NG FA + + N Sbjct: 263 YRKGLLDKEWATMKTKQFE-QKLGNGNVFATAEALWNVGNANALLKTEAADKATEDERQF 321 Query: 337 YKFKMLPSALTKDAKVVS--AGLGFAGTFITKKNKDPETSIKFLQYLASDEGKKLTMFGV 394 Y++K+L + + + LG+ G I++ NKDP ++K L +LAS+EG+ L M+GV Sbjct: 322 YQYKVLAPGVKPEEATYGPKSSLGWDGVAISRSNKDPVRTMKLLDFLASEEGQYLLMWGV 381 Query: 395 EGEHWTWNNEGHPD-------LKYNPSDADFVNGNGIKWWYLYNDGVTEGMLSY-VPGLQ 446 EGEHW + H K N +A G KW ++ +G E Y + G Sbjct: 382 EGEHWEIKDGKHVPKQEVLDAFKKNWDEASRTTGI-RKWTWMIKNGPGEDGTPYDLMGRY 440 Query: 447 KT--------QALMETKAITVYKPQIGLIQAQPDSEEKTIKTKIDEMVKNEKVRIYLANS 498 +T + L +T T +G P E ++TK+++ + +++ L+ S Sbjct: 441 QTDPVTDLAIKNLADTSFDTAPYDNLGPAGGTP---EAIMETKVNDTINKYFLKMALSPS 497 Query: 499 EEEAVANYNSMVKKAEDMGLQKLVDWANATYNKKKDLF 536 E++ V +YN M+ + E GL KL +NK+++L+ Sbjct: 498 EDQVVESYNQMMAEIEAAGLAKLEKIYTDNFNKRQELW 535 >A9U612.1 A9U612_PHYPA Predicted protein Length = 763 Score = 156 bits (394), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 162/588 (27%), Positives = 265/588 (45%), Gaps = 99/588 (16%) Query: 2 KTIRSYRMLTAVLAAIMMISLLSACXXXXXXXXXXXKPDNAAQGDNAGSKDNTGAAGNSG 61 K +++ + + +LAA ++++ L+ C AA GD+ G+ ++ A Sbjct: 218 KGVQAMKTIKGILAAALVMTALTGCGGGGGG--------KAASGDDKGAAADSNAP---- 265 Query: 62 NGEVKELSLFIDAPWYPVK---EWKGPVADKITEKTGVKLKVTVATDD--KQLPLMIASG 116 I W+ + PVA ITEKTGV +++ T + ++L LM+ASG Sbjct: 266 ----------ITLTWFDKNTGDAFNNPVAKAITEKTGVTVEIQQPTGNPVEKLNLMLASG 315 Query: 117 DLPDLVFTYSNVGRMSDSKLSY-------PWNELIEKYAPDFK-IDKTRIAIHTMDDGNF 168 DLPD+V + R SD Y P N+LI+KY P K + +A DDG Sbjct: 316 DLPDVVM----LDRGSDIMNKYISSGAIIPLNDLIDKYGPHIKEMYGDTLAKTKNDDGKN 371 Query: 169 YTVRNSFATQEEMKQNKYSIGSDGNPGIAVREDILKELG------NPPINSLDDFVKVLG 222 Y N + + KY + G +R DI+KELG N + ++F +L Sbjct: 372 YYFANWYGLE------KYPVF-----GFMMRMDIMKELGVGDKVNNGEPFTAEEFENLLV 420 Query: 223 MVKKKYPDM-----VPLIMDKD---WLEQYFLQQFGTGVLLDGWYEQDGKVDYSIRQPKM 274 K+KYP + +P+ ++ + + F FG + +YE +G++ IR P+ Sbjct: 421 KFKEKYPTIDGKESIPMTLNGENIGSVSGTFKGMFG----MMPYYETNGELKKDIRDPRY 476 Query: 275 LEFFKFMNSLYRNGYILAENFALANDQIDDQYATNGKAFAHSHTVATADTDNXXXXXXXX 334 LE KF+NSLYR G + E + Q + + A G FA + + N Sbjct: 477 LEMVKFLNSLYRKGLLDKEWATMKTKQFEQKLA-GGNVFATAEALWNVGNPNSLLKSEAS 535 Query: 335 --------XXYKFKMLPSALTKDAKVVS--AGLGFAGTFITKKNKDPETSIKFLQYLASD 384 Y++K+L ++ D + LG+ G IT+ NKDPE ++K L +LAS+ Sbjct: 536 DPAKADERQFYQYKVLAPGVSADQTTYGPKSSLGWDGVAITRSNKDPERTMKLLDFLASE 595 Query: 385 EGKKLTMFGVEGEHWTWNNEGH---PDL----KYNPSDADFVNGNGIKW-WYLYNDGVTE 436 EG+ L M+GVEG+ W + H P++ K N +A G KW W + N ++ Sbjct: 596 EGQYLLMWGVEGKDWDMKDGKHVPRPEVLDAFKKNWDEASRTTGIR-KWTWMIKNGPGSD 654 Query: 437 GMLSYVPGLQKT--------QALMETKAITVYKPQIGLIQAQPDSEEKTIKTKIDEMVKN 488 G + G ++ + L +T T +G P E + TK+++ + Sbjct: 655 GTPYDLIGRFQSDPVTDLAIKNLADTSFDTAPYDNLGPAGGTP---EAIMDTKVNDTINK 711 Query: 489 EKVRIYLANSEEEAVANYNSMVKKAEDMGLQKLVDWANATYNKKKDLF 536 +++ LA +EE+ YN M+K+ E GL KL Y K++ L+ Sbjct: 712 YFLKMALAPTEEQVTEQYNQMMKEVEAAGLSKLEKIYTENYKKRQQLW 759 >B1DL52.1 B1DL52_9BACL Extracellular solute-binding protein family 1 Length = 520 Score = 134 bits (337), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 136/483 (28%), Positives = 230/483 (47%), Gaps = 78/483 (16%) Query: 4 IRSYRMLTAVLAAIMMISLLSACXXXXXXXXXXXKPDNAAQGDNAGSKDNTGAAGNSGNG 63 + S + + ++L A+M +SL+SAC +A N G+ D +A + Sbjct: 1 MNSKKWMLSLLTAVMSVSLISACSSNSSDKPSSNSNTGSA---NTGTND---SATKDPST 54 Query: 64 EVKELSLFIDAPWYPVKEWKGPVADKITEKTGVKLKVTVATDD--KQLPLMIASGDLPDL 121 + ++ F ++ +A +IT++TGV +++ T + ++L LM+AS DLPD+ Sbjct: 55 DPITITFFDKNT---GDKFDNAIAKEITKRTGVTIEIQQPTGNPTEKLNLMLASDDLPDM 111 Query: 122 V-FTYSN--VGRMSDSKLSYPWNELIEKYAPDFK------IDKTRIAIHTMDDGNFYTVR 172 + F S+ V + +++ P ++LIEKY PD K ++KTR DG Y + Sbjct: 112 IMFDRSSDLVAKYTEAGSIIPLDDLIEKYGPDVKEMYGDVLNKTRYK-----DGKNYYLS 166 Query: 173 NSFATQEEMKQNKYSIGSDGNP--GIAVREDILKEL------GNPPINSLDDFVKVLGMV 224 N + + E P G +R+DI+KEL G P +F ++L Sbjct: 167 NWYGLEHE-------------PVFGFNMRKDIVKELAPDKADGGVPFTQ-SEFKQLLTDF 212 Query: 225 KKKYPDM-----VPLIMD-KDWLEQY--FLQQFGTGVLLDGWYEQDGKVDYSIRQPKMLE 276 K KYP M + L MD ++W F FG L +YE DGK+ Y ++ PK + Sbjct: 213 KAKYPQMDGKDTIALTMDGENWGGTLGTFKGMFG----LMPYYEHDGKLQYDVKDPKYKD 268 Query: 277 FFKFMNSLYRNGYILAENFALANDQIDDQYATNGKAFAHSHT---VATADTDNXXXXXXX 333 +MN LYR+G ++ + +A+ Q+ +Q G F+ + + ++T Sbjct: 269 MILYMNDLYRSG-LMDKEWAINKRQVWEQKIATGTIFSSTGAYWDLGNSNTILKKDKGEE 327 Query: 334 XXXYKFKML-----PSALTKDAKVVSAGLGFAGTFITKKNKDPETSIKFLQYLASDEGKK 388 + +K++ PS T + + LG+ ITKKNK+PE +++F+ +LAS+EG+ Sbjct: 328 SQLFAYKVVADGVDPSKTTYGGR---SSLGWDAIAITKKNKNPERTMQFINFLASEEGQY 384 Query: 389 LTMFGVEGEHWTWNNEGH---PDLKYNPSD--ADFVNGNGI-KW-WYLYNDGVTEGMLSY 441 L M+G+EG HW + H P+ D A + GI KW W++ N +G Sbjct: 385 LLMWGIEGVHWDMKDGKHVPRPETLQAFKDDWAAYTKETGIRKWTWFVKNGPGKDGTPYD 444 Query: 442 VPG 444 +PG Sbjct: 445 LPG 447 >B1D8P2.1 B1D8P2_9BACL Extracellular solute-binding protein family 1 Length = 548 Score = 131 bits (329), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 143/556 (25%), Positives = 234/556 (42%), Gaps = 60/556 (10%) Query: 1 LKTIRSYRMLTAVLAAIMMISLLSACXXXXXXXXXXXKPDNAAQGDNAGSKDNTGAAGNS 60 +K +S +L A +M +++ SAC DN + A S G Sbjct: 1 MKKWKSRALLLAT--CVMCVTVFSACSNTKENT------DNESAPTTAPSAAADGKKSWE 52 Query: 61 GNGEVKELSLFIDAPWYPVKEWKGPVADKITEKTGVKLKVT--VATDDKQLPLMIASGDL 118 + L +++ W+ W A +IT++TGV +K++ V DD++L LMI S L Sbjct: 53 TDTSPITLDAYLNFSWFG-NNWTDSAAKQITKETGVTIKISKPVTDDDQKLNLMINSEKL 111 Query: 119 PDLVFTYSNVGR---MSDSKLSYPWNELIEKYAPDFKIDKTRIAI--HTMDDGNFYTVRN 173 PD++ N M L Y +EL +YAP+ D + A + DDG+ Y + Sbjct: 112 PDIIMADKNSPSWSIMEQKGLLYSIDELAAQYAPELLQDAPKEAFTNYKADDGHTYMYVD 171 Query: 174 SF---ATQEEMKQNKYSIGSDGNPGIAVREDILKELGNPPINSLDDFVKVLGMVKKKYPD 230 Q+E K+ IG++ P ++R+D L E+GNP I++ D FV L +K K+PD Sbjct: 172 FIEGEQYQQEAKKYNALIGTN-QPEWSIRQDYLDEIGNPDISTPDAFVAALDKIKAKHPD 230 Query: 231 MVPLIMDKDWLEQYFLQQFGTGVLLDGW-YEQDG-KVDYSIRQPKMLEFFKFMNSLYRNG 288 + + L Q GT + + Y DG ++ +R + + FMN L Sbjct: 231 KMGFYANVGDLANDRDYQMGTNSMFSNYRYYVDGDQIKSGLRSDSFKQIYSFMNELNTKE 290 Query: 289 YILAENFALANDQIDDQYATNGKAFAHSHTVATADTDNXXXXXXXXXXYKFKMLPSALTK 348 + + F D I Q +GK +++ + YK M P A Sbjct: 291 LLTKDTFIDTKD-IFTQKINSGKTISYAWAIGEG---TKSPADNPKTSYKV-MDPFATYT 345 Query: 349 DAKVVSAGLGFAGTFITKKNKDPETSIKFLQYLASDEGKKLTMFGVEGEHWTWNNEGHPD 408 + G G+ I K NK PE +I+FL Y+AS +G +GVEG+ ++ + P Sbjct: 346 Q---IRTGGGWNAFAIPKSNKHPERTIRFLAYMASKQGHIAAKWGVEGDKFSGDVVNGPH 402 Query: 409 LK-------YNP----------SDADFVNGNGIKW------WYLYNDGVTEGMLSYVPGL 445 Y P S NG G W W+LY + P Sbjct: 403 YSIVDDKPLYLPEYWDVKQKDWSGVSAKNGLGEYWFATNTAWWLYPEWNNTD-----PSF 457 Query: 446 QKTQALMETKAITVYKPQIGLIQAQPDSEEKTIKTKIDEMVKNEKVRIYLANSEEEAVAN 505 + + K Y P++ + P+S+E I ++ ++ V++ A SE E+ + Sbjct: 458 IQYNKMFADKV--KYMPELENLDPLPNSKEGIILKRVTDLYAQYGVKMIFAKSEAESESV 515 Query: 506 YNSMVKKAEDMGLQKL 521 Y ++KA+ +GL K+ Sbjct: 516 YKEFIEKADSLGLPKV 531 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,199,962,294 Number of extensions: 141527892 Number of successful extensions: 276543 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 276414 Number of HSP's successfully gapped: 80 Length of query: 537 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 396 Effective length of database: 1,732,333,088 Effective search space: 686003902848 Effective search space used: 686003902848 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 134 (56.2 bits)