BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0010.p (382 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A4F259.1 A4F259_9BACL Putative exo-oligoxylanase 603 e-170 B1DF36.1 B1DF36_9BACL Glycoside hydrolase family 8 580 e-163 Q9KB30.1 Q9KB30_BACHD Xylanase Y 485 e-135 Q8GLI2.1 Q8GLI2_BACHD Xylanase Y (Fragment) 481 e-134 C4ZGK5.1 C4ZGK5_9FIRM Xylanase Y 439 e-121 >A4F259.1 A4F259_9BACL Putative exo-oligoxylanase Length = 380 Score = 603 bits (1554), Expect = e-170, Method: Compositional matrix adjust. Identities = 279/380 (73%), Positives = 317/380 (83%) Query: 1 MNIFTEGSFYTGKYRNLFRENGISEEAVRHKLDDTWNELFYGAPDVRIYHPMGDDKGYIV 60 MNI +G++ T Y NLF + G E+ + +L+++WNELF G D RIY+PMG+DKGY++ Sbjct: 1 MNITGQGAYDTNTYNNLFHKFGYDEQEINARLEESWNELFDGEEDTRIYYPMGEDKGYLL 60 Query: 61 DTGNTDVRTEGMSYGMMMAVQMNKQEEFDRLWKFAKVYMQHTEGRYKDYFAWHCKPDGTR 120 DTGN DVR+EGMSYGMMMAVQM+K+EEFDRLW F+ +MQH EGRYKDYFAWHCKPDGTR Sbjct: 61 DTGNNDVRSEGMSYGMMMAVQMDKKEEFDRLWNFSHAFMQHAEGRYKDYFAWHCKPDGTR 120 Query: 121 LSPGPAPDGEEFFAMALFFASKRWGDGAEPFNYSEQARIILRACVHKGENEDGDPMWDPA 180 LS GPAPDGEEFFAMALFFAS RWGDG P NY EQAR ILRAC+H+GEN +GDPMWDP Sbjct: 121 LSQGPAPDGEEFFAMALFFASNRWGDGDAPLNYKEQARRILRACIHQGENGEGDPMWDPE 180 Query: 181 TKLIKFVPETPFSDPSYHLPHFYELFALQADEQDRAFWKEAAARSRAYLHKACHPVTGLS 240 TKLIKFVPE+PFSDPSYHLPHFYELFAL ADE D++FW++AAA SRAYLH ACHPVTGLS Sbjct: 181 TKLIKFVPESPFSDPSYHLPHFYELFALYADEDDQSFWRDAAAASRAYLHTACHPVTGLS 240 Query: 241 PEYANYDGTPAEPQPHGDFRHFFSDAYRVAANIGLDYEWFRCDPWQTGQSNRIQAFFRDI 300 PEYANYDG+PA QPHGDFRHF+SDAYRVAANI LD+EWF DPWQ QSNRIQ+FF I Sbjct: 241 PEYANYDGSPAPVQPHGDFRHFYSDAYRVAANIALDWEWFGKDPWQVEQSNRIQSFFSSI 300 Query: 301 DAADYRRYTIDGQPFEEPSLHPVGXXXXXXXXXXXXDGPDAGHYVKLFWNTPLRTGVRRY 360 D A+YRRYTI+G+PF+EPSLHP+G DGP H+V+LFWNTPLR G RRY Sbjct: 301 DVAEYRRYTIEGEPFDEPSLHPIGLLATNAMASLAADGPHVEHFVRLFWNTPLRQGERRY 360 Query: 361 YDNCLYFFSLLALSGNYRIY 380 YDNCLYFFSLLALSG YRIY Sbjct: 361 YDNCLYFFSLLALSGKYRIY 380 >B1DF36.1 B1DF36_9BACL Glycoside hydrolase family 8 Length = 382 Score = 580 bits (1494), Expect = e-163, Method: Compositional matrix adjust. Identities = 280/381 (73%), Positives = 319/381 (83%), Gaps = 1/381 (0%) Query: 1 MNIFTEGSFYTGKYRNLFRENGISEEAVRHKLDDTWNELFYGAPDVRIYHPMGDDKGYIV 60 M +G++YTG+YRNLF+E G +E A++ KL+ WN+L +G PD+RIY+PMG+DKGY + Sbjct: 1 MTALEKGAYYTGEYRNLFKELGYAETAIQAKLEQAWNDLLHGDPDIRIYYPMGEDKGYFL 60 Query: 61 DTGNTDVRTEGMSYGMMMAVQMNKQEEFDRLWKFAKVYMQHTEGRYKDYFAWHCKPDGTR 120 DTGN DVR+EGMSYGMMMAVQMN +E FDRLW F+ YMQH EGRYKDYFAWHCKPDGTR Sbjct: 61 DTGNVDVRSEGMSYGMMMAVQMNDKEIFDRLWNFSVTYMQHAEGRYKDYFAWHCKPDGTR 120 Query: 121 LSPGPAPDGEEFFAMALFFASKRWGDGAEPFNYSEQARIILRACVHKGENEDG-DPMWDP 179 LS GPAPDGEEFFAMALFFA+ RWG+G EP+NYSEQA ILRAC+H+GE+ +G PMW+P Sbjct: 121 LSQGPAPDGEEFFAMALFFAANRWGNGPEPYNYSEQASRILRACIHQGEDGNGGSPMWEP 180 Query: 180 ATKLIKFVPETPFSDPSYHLPHFYELFALQADEQDRAFWKEAAARSRAYLHKACHPVTGL 239 A KLIKFVPE+PFSDPSYHLPHFYELF+L ADE DR FW+EAA+ SR YLH ACHP TGL Sbjct: 181 ANKLIKFVPESPFSDPSYHLPHFYELFSLWADEVDRPFWREAASASRTYLHTACHPETGL 240 Query: 240 SPEYANYDGTPAEPQPHGDFRHFFSDAYRVAANIGLDYEWFRCDPWQTGQSNRIQAFFRD 299 SPEYANYDGTPAE Q HGDFRHF+SDAYRVAANIGLD+ WFR DPWQ QSNRIQ FFR Sbjct: 241 SPEYANYDGTPAEVQRHGDFRHFYSDAYRVAANIGLDWAWFRRDPWQVEQSNRIQRFFRP 300 Query: 300 IDAADYRRYTIDGQPFEEPSLHPVGXXXXXXXXXXXXDGPDAGHYVKLFWNTPLRTGVRR 359 +D A YRRYTIDGQPF+EP+LHP+G DGPDA H+V+L WNTPLR G RR Sbjct: 301 VDLAAYRRYTIDGQPFDEPALHPIGLLATNAMASLAADGPDADHFVQLLWNTPLRKGERR 360 Query: 360 YYDNCLYFFSLLALSGNYRIY 380 YYDNCLYFFSLLALSG+YRIY Sbjct: 361 YYDNCLYFFSLLALSGSYRIY 381 >Q9KB30.1 Q9KB30_BACHD Xylanase Y Length = 388 Score = 485 bits (1249), Expect = e-135, Method: Compositional matrix adjust. Identities = 238/383 (62%), Positives = 284/383 (74%), Gaps = 5/383 (1%) Query: 1 MNIFTEGSFYTGKYRNLFRENGISEEAVRHKLDDTWNELFYGAPDVRIYHPMGDDKGYIV 60 M TEG+FYT +YRNLF+E G SE ++ ++ DTW +LF P+ +IY+ +GDD GY++ Sbjct: 1 MKKTTEGAFYTREYRNLFKEFGYSEAEIQERVKDTWEQLFGDNPETKIYYEVGDDLGYLL 60 Query: 61 DTGNTDVRTEGMSYGMMMAVQMNKQEEFDRLWKFAKVYMQHTEGRYKDYFAWHCKPDGTR 120 DTGN DVRTEGMSYGMMMAVQM++++ FDR+W + M TEG + YFAW C+PDGT+ Sbjct: 61 DTGNLDVRTEGMSYGMMMAVQMDRKDIFDRIWNWTMKNMYMTEGVHAGYFAWSCQPDGTK 120 Query: 121 LSPGPAPDGEEFFAMALFFASKRWGDGAE-PFNYSEQARIILRACVHKGENEDGDPMWDP 179 S GPAPDGEE+FA+ALFFAS RWGDG E PFNYSEQAR +L CVH GE G PMW+ Sbjct: 121 NSWGPAPDGEEYFALALFFASHRWGDGDEQPFNYSEQARKLLHTCVHNGEGGPGHPMWNR 180 Query: 180 ATKLIKFVPETPFSDPSYHLPHFYELFALQADEQDRAFWKEAAARSRAYLHKACHPVTGL 239 KLIKF+PE FSDPSYHLPHFYELF+L A+E+DR FWKEAA SR YL ACHP TGL Sbjct: 181 DNKLIKFIPEVEFSDPSYHLPHFYELFSLWANEEDRVFWKEAAEASREYLKIACHPETGL 240 Query: 240 SPEYANYDGTPAEPQPHGDFRHFFSDAYRVAANIGLDYEWFRCDPWQTGQSNRIQAFFRD 299 +PEYA YDGTP + + +G HFFSD+YRVAANIGLD EWF W + N+IQAFF D Sbjct: 241 APEYAYYDGTPNDEKGYG---HFFSDSYRVAANIGLDAEWFGGSEWSAEEINKIQAFFAD 297 Query: 300 IDAADYRRYTIDGQPFEEPSLHPVGXXXXXXX-XXXXXDGPDAGHYVKLFWNTPLRTGVR 358 + DYRRY IDG+PFEE SLHPVG DGP A V LFWNTP+RTG R Sbjct: 298 KEPEDYRRYKIDGEPFEEKSLHPVGLIATNAMGSLASVDGPYAKANVDLFWNTPVRTGNR 357 Query: 359 RYYDNCLYFFSLLALSGNYRIYI 381 RYYDNCLY F++LALSGN++I+ Sbjct: 358 RYYDNCLYLFAMLALSGNFKIWF 380 >Q8GLI2.1 Q8GLI2_BACHD Xylanase Y (Fragment) Length = 374 Score = 481 bits (1238), Expect = e-134, Method: Compositional matrix adjust. Identities = 236/375 (62%), Positives = 280/375 (74%), Gaps = 5/375 (1%) Query: 5 TEGSFYTGKYRNLFRENGISEEAVRHKLDDTWNELFYGAPDVRIYHPMGDDKGYIVDTGN 64 TEG+FYT +YRNLF+E G SE ++ ++ DTW +LF P+ +IY+ +GDD GY++DTGN Sbjct: 3 TEGAFYTREYRNLFKEFGYSEAEIQERVKDTWEQLFGDNPETKIYYEVGDDLGYLLDTGN 62 Query: 65 TDVRTEGMSYGMMMAVQMNKQEEFDRLWKFAKVYMQHTEGRYKDYFAWHCKPDGTRLSPG 124 DVRTEGMSYGMMMAVQM++++ FDR+W + M TEG + YFAW C+PDGT+ S G Sbjct: 63 LDVRTEGMSYGMMMAVQMDRKDIFDRIWNWTMKNMYMTEGVHAGYFAWSCQPDGTKNSWG 122 Query: 125 PAPDGEEFFAMALFFASKRWGDGAE-PFNYSEQARIILRACVHKGENEDGDPMWDPATKL 183 PAPDGEE+FA+ALFFAS RWGDG E PFNYSEQAR +L CVH GE G PMW+ KL Sbjct: 123 PAPDGEEYFALALFFASHRWGDGDEQPFNYSEQARKLLHTCVHNGEGGPGHPMWNRDNKL 182 Query: 184 IKFVPETPFSDPSYHLPHFYELFALQADEQDRAFWKEAAARSRAYLHKACHPVTGLSPEY 243 IKF+PE FSDPSYHLPHFYELF+L A+E+DR FWKEAA SR YL ACHP TGL+PEY Sbjct: 183 IKFIPEVEFSDPSYHLPHFYELFSLWANEEDRVFWKEAAEASREYLKIACHPETGLAPEY 242 Query: 244 ANYDGTPAEPQPHGDFRHFFSDAYRVAANIGLDYEWFRCDPWQTGQSNRIQAFFRDIDAA 303 A YDGTP + + +G HFFSD+YRVAANIGLD EWF W + N+IQAFF D + Sbjct: 243 AYYDGTPNDEKGYG---HFFSDSYRVAANIGLDAEWFGGSEWSAEEINKIQAFFADKEPE 299 Query: 304 DYRRYTIDGQPFEEPSLHPVGXXXXXXX-XXXXXDGPDAGHYVKLFWNTPLRTGVRRYYD 362 DYRRY IDG+PFEE SLHPVG DGP A V LFWNTP+RTG RRYYD Sbjct: 300 DYRRYKIDGEPFEEKSLHPVGLIATNAMGSLASVDGPYAKANVDLFWNTPVRTGNRRYYD 359 Query: 363 NCLYFFSLLALSGNY 377 NCLY F++LALSGN+ Sbjct: 360 NCLYLFAMLALSGNF 374 >C4ZGK5.1 C4ZGK5_9FIRM Xylanase Y Length = 380 Score = 439 bits (1130), Expect = e-121, Method: Compositional matrix adjust. Identities = 219/378 (57%), Positives = 268/378 (70%), Gaps = 4/378 (1%) Query: 7 GSFYTGKYRNLFRENGISEEAVRHKLDDTWNELFYGAPDVRIYHPMGDDKGYIVDTGNTD 66 G++ TGKYRN+F+E G EE ++ +++DT+N +FYG D R YH GDD GY+ DTGN D Sbjct: 3 GAYKTGKYRNIFKEYGYPEEEIQKRVEDTFNTIFYGNDDERFYHEAGDDMGYMEDTGNHD 62 Query: 67 VRTEGMSYGMMMAVQMNKQEEFDRLWKFAKVYMQHTEGRYKDYFAWHCKPDGTRLSPGPA 126 VRTEGMSYGMM+ VQ++K+ EFDRLWK+ + YM +G K+YFAW C+ +G R + GPA Sbjct: 63 VRTEGMSYGMMVCVQLDKKAEFDRLWKWVRTYMYIEDGPAKNYFAWSCQTNGKRNADGPA 122 Query: 127 PDGEEFFAMALFFASKRWGDGAEPFNYSEQARIILRACVHKGE-NEDGDPMWDPATKLIK 185 PDGEE+FAMALFFASKRWGDG F YS++A+ ILR CVHKGE G+PMW+P+ KLIK Sbjct: 123 PDGEEYFAMALFFASKRWGDGDGIFCYSKEAQNILRECVHKGEPGHSGEPMWEPSNKLIK 182 Query: 186 FVPETPFSDPSYHLPHFYELFALQADEQDRAFWKEAAARSRAYLHKACHPVTGLSPEYAN 245 FV FSDPSYHLPHFYELFA +A E+DR FWKEAAA SRAYLH ACH TGLS EYA+ Sbjct: 183 FVTNMDFSDPSYHLPHFYELFAQRAYEEDRDFWKEAAAASRAYLHSACHEKTGLSAEYAD 242 Query: 246 YDGTPAEPQPHGDFRH--FFSDAYRVAANIGLDYEWFRCDPWQTGQSNRIQAFFRDIDAA 303 YDG P RH ++SDAYR ANI +D+ WF DPWQ + R+Q F+ + Sbjct: 243 YDGKPHTGHQEIFGRHDWYYSDAYRTIANIAMDHLWFDADPWQIKTAERLQKFYCEDQKD 302 Query: 304 DYR-RYTIDGQPFEEPSLHPVGXXXXXXXXXXXXDGPDAGHYVKLFWNTPLRTGVRRYYD 362 ++ + IDG P +E +LHPV DG A V+ FWNTPLRTG RRYYD Sbjct: 303 NWDGVFLIDGTPLDEKALHPVAIIAVNAEASLAADGKYAKQCVEKFWNTPLRTGDRRYYD 362 Query: 363 NCLYFFSLLALSGNYRIY 380 N LY F++LALSGNYRIY Sbjct: 363 NFLYMFAMLALSGNYRIY 380 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.459 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,610,678,518 Number of extensions: 116624249 Number of successful extensions: 208337 Number of sequences better than 1.0e-05: 55 Number of HSP's gapped: 208208 Number of HSP's successfully gapped: 55 Length of query: 382 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 245 Effective length of database: 1,770,017,148 Effective search space: 433654201260 Effective search space used: 433654201260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)