BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0003.p (319 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KC04.1 B3KC04_9BACI Aldo/keto reductase 401 e-110 B1DGG4.1 B1DGG4_9BACL Aldo/keto reductase 370 e-101 B1ZS31.1 B1ZS31_OPITP Aldo/keto reductase 321 6e-86 Q15XR1.1 Q15XR1_PSEA6 Aldo/keto reductase 296 1e-78 A5JPK6.1 A5JPK6_VITVI L-galactose dehydrogenase 294 7e-78 >B3KC04.1 B3KC04_9BACI Aldo/keto reductase Length = 313 Score = 401 bits (1031), Expect = e-110, Method: Compositional matrix adjust. Identities = 192/310 (61%), Positives = 230/310 (74%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 MKYRKLG TGL+ RE E I TVH A+D GINLIDVSPYYGL K Sbjct: 1 MKYRKLGRTGLDVSVLGFGASSLGSVFRETDDSEGIRTVHAAMDAGINLIDVSPYYGLTK 60 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILLLH 120 AETVLGEA++ +PRD++ILSTKAGRYGE +FDF+ +RII S +ES+ RL TDY+DIL LH Sbjct: 61 AETVLGEAIRQLPRDQFILSTKAGRYGENDFDFTSKRIIASIDESLKRLQTDYVDILFLH 120 Query: 121 DVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVLPQTRLDVILSYCHYSL 180 D+EF P VMEE +PAL+ LK+ GKIR+ G+ GLPL +FEK+LPQ +D I+SYCHYSL Sbjct: 121 DIEFVPASVVMEEALPALQLLKEKGKIRFGGICGLPLQLFEKLLPQIEVDAIISYCHYSL 180 Query: 181 NDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEYCRDKG 240 ND T +K G GL+NASP+SMGLL +R P WHPA A+I+ AC+RAAEYC G Sbjct: 181 NDQTLTGLLPLLDKEGIGLINASPLSMGLLGSRGAPSWHPASADIREACRRAAEYCAKAG 240 Query: 241 EDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDILKPIDGAT 300 DIAKLAVQFST+NEQIPTTLVSTA P NI+ N W+EEP+D+ LL EV IL+P+ G T Sbjct: 241 ADIAKLAVQFSTSNEQIPTTLVSTANPDNIQRNAAWSEEPLDQTLLAEVLKILEPVQGRT 300 Query: 301 WPSGRAEWNE 310 W SGR E+NE Sbjct: 301 WTSGRPEYNE 310 >B1DGG4.1 B1DGG4_9BACL Aldo/keto reductase Length = 311 Score = 370 bits (950), Expect = e-101, Method: Compositional matrix adjust. Identities = 184/310 (59%), Positives = 217/310 (70%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 MKYRKLG TGLE RE +E+I TVH AID GINLIDVSPYYGL K Sbjct: 1 MKYRKLGKTGLEVSVLSFGASSLGSVFRETDDNESIRTVHAAIDAGINLIDVSPYYGLKK 60 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILLLH 120 AETVLG+AL+++PRD+Y LSTKAGRYG FDF E II S EES+ RL TDYLDIL LH Sbjct: 61 AETVLGQALKSIPRDRYYLSTKAGRYGADLFDFRSETIIASVEESLQRLHTDYLDILFLH 120 Query: 121 DVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVLPQTRLDVILSYCHYSL 180 D+EF + EE IPA++ LK GKIR G+ GLPLS+FE+ LP +D I+SYCHYSL Sbjct: 121 DIEFVEPAIIHEEAIPAIQRLKAEGKIRSTGICGLPLSLFEEFLPNADIDAIISYCHYSL 180 Query: 181 NDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEYCRDKG 240 NDTT E+ GL+NASP+SMGLL R PDWHPA E++ C+ AAE+C +G Sbjct: 181 NDTTLETILPLIEEKNIGLVNASPLSMGLLGTRGTPDWHPASREVKEICKWAAEHCAAQG 240 Query: 241 EDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDILKPIDGAT 300 EDIAKLAVQFST NE+IPTTLVSTA P+NI N W +EP++ +LL EVR IL PI T Sbjct: 241 EDIAKLAVQFSTMNERIPTTLVSTANPSNIVKNAIWVDEPINLRLLEEVRQILAPIMNET 300 Query: 301 WPSGRAEWNE 310 W SG +N+ Sbjct: 301 WASGIPFYNK 310 >B1ZS31.1 B1ZS31_OPITP Aldo/keto reductase Length = 317 Score = 321 bits (822), Expect = 6e-86, Method: Compositional matrix adjust. Identities = 154/310 (49%), Positives = 207/310 (66%), Gaps = 3/310 (0%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 M R+LGNTGL+ R E+I TVHTA+DLGIN IDVSPYYG + Sbjct: 1 MHRRRLGNTGLDVSILSFGASSLGGVFRTTDDAESIRTVHTAVDLGINFIDVSPYYGATR 60 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILLLH 120 AETVLG+AL+ +PRD+Y L+TKAG+YGEGEFDFS R+ RS +ES RLG DY+D+L H Sbjct: 61 AETVLGKALREIPRDRYFLATKAGQYGEGEFDFSAARVTRSLDESCARLGVDYIDLLQCH 120 Query: 121 DVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVLPQT---RLDVILSYCH 177 D+EF ++Q++ E IPAL +LKQAG+I + G++GLPL +F V+ + ++ +LS+CH Sbjct: 121 DIEFASLDQIVTETIPALLQLKQAGRIGHIGITGLPLKIFPAVIDRVAPGAVETVLSFCH 180 Query: 178 YSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEYCR 237 Y LND++ E+ G++NASPI MGLL+ R VP WHPA + A +RA E+C+ Sbjct: 181 YELNDSSLESLLPYFEQKRVGVINASPIGMGLLTPRGVPSWHPAPPALVAGARRAVEFCQ 240 Query: 238 DKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDILKPID 297 G DI KLA+QF+ A+ I TTLV +A+ NIR NI + EP+D +LL V +IL+PI Sbjct: 241 SVGADIVKLAIQFAVAHPGIATTLVGSASSENIRRNIAYASEPVDRELLARVLEILQPIH 300 Query: 298 GATWPSGRAE 307 + GR E Sbjct: 301 NHNFTRGRPE 310 >Q15XR1.1 Q15XR1_PSEA6 Aldo/keto reductase Length = 312 Score = 296 bits (759), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 146/306 (47%), Positives = 194/306 (63%), Gaps = 3/306 (0%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 M YR+LG T L+ R + E I TVHTA+DLGIN +DVSPYYG Sbjct: 1 MNYRQLGKTDLKLSQVSYGASPLGSVFRNISEQEGIKTVHTALDLGINYLDVSPYYGDTV 60 Query: 61 AETVLGEALQTVPRDKYILSTKAGRYGE--GEFDFSRERIIRSAEESMGRLGTDYLDILL 118 AETVLG+AL+ +PRD+YILSTKAGRYG +FDFS +RI S +ES RLG + +DIL Sbjct: 61 AETVLGKALKGIPRDQYILSTKAGRYGADFADFDFSAKRIEASLDESCSRLGVNEVDILF 120 Query: 119 LHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVLPQTRLDVILSYCHY 178 LHD+EF + QV++E IP L LK+AGKIRY GV+G PL VF +V+ Q +D IL+YC Y Sbjct: 121 LHDIEFADMRQVLQESIPCLLALKKAGKIRYAGVTGYPLKVFSEVISQYEIDCILTYCRY 180 Query: 179 SLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEYCRD 238 +L D + +++ G++NASP MGLL+ R P WHP +++ A +A C+ Sbjct: 181 ALYDNSLAEIIPTLDEASVGIINASPTGMGLLTERGAPQWHPGSEQLKQASLKAVSLCQS 240 Query: 239 KGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEEPMDEQLLTEVRDILKPIDG 298 KG DI LA+QF+ + I +TLV TA PANI N++W D L++E++ I + Sbjct: 241 KGIDITALALQFAIDHPSIASTLVGTANPANIVKNVEWASAQPDASLVSEIQQIFANVP- 299 Query: 299 ATWPSG 304 TWPSG Sbjct: 300 CTWPSG 305 >A5JPK6.1 A5JPK6_VITVI L-galactose dehydrogenase Length = 319 Score = 294 bits (753), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 150/313 (47%), Positives = 204/313 (65%), Gaps = 10/313 (3%) Query: 1 MKYRKLGNTGLEXXXXXXXXXXXXXXXREVPRDEAILTVHTAIDLGINLIDVSPYYGLMK 60 ++ R LG TGL+ V D+AI +V A LGIN D SPYYG Sbjct: 4 LELRDLGTTGLKLSCVGFGASPLGNVFGLVSEDDAIASVREAFRLGINFFDTSPYYGGTL 63 Query: 61 AETVLGEALQTV--PRDKYILSTKAGRYGEGEFDFSRERIIRSAEESMGRLGTDYLDILL 118 +E VLG+ L+ V PR++YI+STK GRY EG FDFS ERI RS +ES+ RL DY+DIL Sbjct: 64 SEKVLGKCLKAVGMPRNEYIVSTKCGRYAEG-FDFSAERITRSIDESLARLQLDYVDILQ 122 Query: 119 LHDVEFGPVEQVMEEGIPALEELKQAGKIRYYGVSGLPLSVFEKVL---PQTRLDVILSY 175 HD+EFG ++QV+ E IPAL++LK+AGKIR+ G++GLPL +F VL P +DVILSY Sbjct: 123 CHDIEFGSLDQVVNETIPALQKLKEAGKIRFIGITGLPLEIFTYVLDRVPPGSVDVILSY 182 Query: 176 CHYSLNDTTXXXXXXXXEKSGTGLMNASPISMGLLSNRKVPDWHPAGAEIQAACQRAAEY 235 CHYS+NDTT + G G+++ASP++MGLL+ R P+WHPA E+++ACQ AA + Sbjct: 183 CHYSINDTTLEDLLPYLKSKGVGVISASPLAMGLLTERGPPEWHPASPELKSACQAAAAH 242 Query: 236 CRDKGEDIAKLAVQFSTANEQIPTTLVSTATPANIRNNIKWTEE----PMDEQLLTEVRD 291 C++KG++I+KLA+QFS +N+ I + LV + + N+ E DE+ L+EV Sbjct: 243 CKEKGKNISKLAMQFSLSNKDISSVLVGMNSIKQVEENVAAATELAIFGKDEETLSEVEA 302 Query: 292 ILKPIDGATWPSG 304 IL P+ TWPSG Sbjct: 303 ILNPVKNQTWPSG 315 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,822,615,172 Number of extensions: 73992227 Number of successful extensions: 145782 Number of sequences better than 1.0e-05: 5152 Number of HSP's gapped: 140919 Number of HSP's successfully gapped: 5159 Length of query: 319 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 184 Effective length of database: 1,788,859,178 Effective search space: 329150088752 Effective search space used: 329150088752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)