BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0002.p (339 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KC05.1 B3KC05_9BACI Alcohol dehydrogenase GroES domain protein 471 e-131 B1DGG3.1 B1DGG3_9BACL Alcohol dehydrogenase GroES domain protein 457 e-127 Q5WKX5.1 Q5WKX5_BACSK Zn-dependent alcohol dehydrogenase 424 e-117 A3I1Q8.1 A3I1Q8_9SPHI Zn-dependent alcohol dehydrogenase 370 e-101 A5FCD7.1 A5FCD7_FLAJ1 Alcohol dehydrogenase, zinc-binding domain... 359 2e-97 >B3KC05.1 B3KC05_9BACI Alcohol dehydrogenase GroES domain protein Length = 337 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 212/338 (62%), Positives = 274/338 (81%), Gaps = 1/338 (0%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 MKGI+C+E+GKF +REDLPEP L +GEAI+ I+RIGICGTDLHA+RGNQP+FTYPR+LGH Sbjct: 1 MKGIVCQEIGKFQYREDLPEPLLLEGEAIVRIKRIGICGTDLHAFRGNQPFFTYPRILGH 60 Query: 61 EVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMRE 120 E+SGIIE++G N +GL+ GD V VIPY+HCGEC CL GKTNCC+ ++V GVH+DGGMRE Sbjct: 61 ELSGIIEQVGTNEEGLKPGDTVCVIPYIHCGECMPCLRGKTNCCRSLKVMGVHIDGGMRE 120 Query: 121 RVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAMA 180 R+S+P R+L++ +GLTLDQAA++EPLAIGAH V+R+ ++ D VLVIGAGPIGLG+M A Sbjct: 121 RISVPTRHLMRAEGLTLDQAALVEPLAIGAHGVKRANVTPKDTVLVIGAGPIGLGIMVFA 180 Query: 181 RYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNIS 240 + G VIAMD+N +RL FCK WA+ +H + E + LSALTDG F V+D+TGN S Sbjct: 181 KALGATVIAMDMNADRLAFCKTWAKVDHVIQVGDELSD-LSALTDGEFPSIVLDATGNQS 239 Query: 241 SMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRDG 300 SM AF +V HGGTL YVGL++G+I+F DP+FH RE+T+ GSRNATR+DF V+ I +G Sbjct: 240 SMTNAFNMVAHGGTLVYVGLIQGDISFRDPEFHKRELTLMGSRNATRDDFHDVMKRISEG 299 Query: 301 YIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 +DVD YI+HRC F ++I +F+ WL+P +KVIKA+VEL Sbjct: 300 GMDVDPYITHRCRFDDLIQEFQGWLRPSAKVIKAMVEL 337 >B1DGG3.1 B1DGG3_9BACL Alcohol dehydrogenase GroES domain protein Length = 339 Score = 457 bits (1176), Expect = e-127, Method: Compositional matrix adjust. Identities = 212/337 (62%), Positives = 267/337 (79%), Gaps = 1/337 (0%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 MKGI+CE+V +F + DL EP GEAI+SI+R+GICGTDLHAY+GNQP+FTYPR+LGH Sbjct: 2 MKGIVCEQVEQFRY-ADLEEPVFTPGEAIVSIKRVGICGTDLHAYKGNQPFFTYPRILGH 60 Query: 61 EVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMRE 120 E+SG+IE+IGDN +G +AGDQVS+IPYLHCG+C AC GKTNCC ++V GVH DGGMRE Sbjct: 61 ELSGLIEQIGDNEEGFKAGDQVSIIPYLHCGKCVACRQGKTNCCTSIQVLGVHADGGMRE 120 Query: 121 RVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAMA 180 ++S+PV +LL DGL+L+QAA++EP +IGAHAVRRSG++AGD VLVIGAGPIGLG+MA+A Sbjct: 121 KISVPVTHLLNADGLSLEQAAVVEPFSIGAHAVRRSGLAAGDTVLVIGAGPIGLGIMALA 180 Query: 181 RYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNIS 240 R+AG VIAMDVN+ERL FC+ WA+ +H V+AL P +L ALT G++ V D+TGN Sbjct: 181 RHAGANVIAMDVNNERLDFCQEWAKVDHIVNALDAPFVRLEALTGGDYPTIVFDATGNTH 240 Query: 241 SMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRDG 300 SM A HGGTL YVGLVKG I+F D +FH RE+T+ GSRNAT+ED + V+ IR G Sbjct: 241 SMSAAVRYAAHGGTLVYVGLVKGYISFDDAEFHKRELTIMGSRNATKEDCQHVLEVIRAG 300 Query: 301 YIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVE 337 ID+D Y++H F EM FESWL P +KVIKA++E Sbjct: 301 GIDIDGYVTHLVPFDEMADHFESWLDPANKVIKAMIE 337 >Q5WKX5.1 Q5WKX5_BACSK Zn-dependent alcohol dehydrogenase Length = 337 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 198/338 (58%), Positives = 249/338 (73%), Gaps = 1/338 (0%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 M+ I+CE F ++ P +R GEA++ I+RIGICGTDLHAY+GNQP+F+YPRVLGH Sbjct: 1 MRSIVCERPNVFKMTKE-KAPSIRPGEALVRIKRIGICGTDLHAYKGNQPFFSYPRVLGH 59 Query: 61 EVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMRE 120 E+SG+IE + NP+GL+AGD+V++IPYL CG C AC +GKTNCC K+ V GVH+DGGMRE Sbjct: 60 ELSGVIEEVEHNPEGLQAGDRVAIIPYLSCGNCHACRNGKTNCCTKVNVLGVHIDGGMRE 119 Query: 121 RVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAMA 180 V++P + L+KT L+ D+AA++EPLAIGAHAV R+ +QVLVIGAGPIGLGVM A Sbjct: 120 LVAVPTKQLMKTTSLSYDEAALIEPLAIGAHAVNRAKPRRNEQVLVIGAGPIGLGVMLFA 179 Query: 181 RYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNIS 240 + G +VIAMDVN+ERL FC+ WA+ + V AL P +L+ + V D+TGN Sbjct: 180 KEQGARVIAMDVNEERLEFCQQWAKVDAVVHALDNPLSELNDRLNDELPTIVFDATGNND 239 Query: 241 SMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRDG 300 SM AF V HGG L YVGLV G I F DP+FH REMT+ SRNAT+EDF VI + G Sbjct: 240 SMTEAFNYVAHGGKLVYVGLVNGMIQFDDPEFHKREMTLMASRNATKEDFNYVIQVLETG 299 Query: 301 YIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 I +DSYI+HRCSF EM+ QF+SWL PESKVIK +VEL Sbjct: 300 SIQLDSYITHRCSFREMVEQFDSWLLPESKVIKVVVEL 337 >A3I1Q8.1 A3I1Q8_9SPHI Zn-dependent alcohol dehydrogenase Length = 336 Score = 370 bits (950), Expect = e-101, Method: Compositional matrix adjust. Identities = 178/338 (52%), Positives = 234/338 (69%), Gaps = 2/338 (0%) Query: 1 MKGIICEEVGKFVFREDLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLGH 60 MK + C+ G+F + E P+L +G AI+ I+RIGICGTDLHA+ G QP+F+YPR LGH Sbjct: 1 MKVLSCKSPGEFEYLES-KMPKLSEGRAIVKIKRIGICGTDLHAFEGTQPFFSYPRTLGH 59 Query: 61 EVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMRE 120 E+SG + IG++ + + GD V+VIPY +CG C AC GK NCCQ + VFGVH DGGM+E Sbjct: 60 ELSGEVIEIGESEEIFK-GDDVTVIPYFNCGSCIACKKGKPNCCQNISVFGVHEDGGMQE 118 Query: 121 RVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAMA 180 VSLP+ +L+K +GL+L+Q A+ EP AIGAH V R+G+ G VLV+GAGPIGLGV+ A Sbjct: 119 YVSLPIPSLVKKEGLSLEQLALAEPFAIGAHGVSRAGVKPGHVVLVVGAGPIGLGVLEFA 178 Query: 181 RYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEPKEQLSALTDGNFLPHVIDSTGNIS 240 R AG VIAMD N++RL FC+ EH V A E + +T G+F VID+TG+++ Sbjct: 179 RIAGATVIAMDTNEKRLEFCQKEWNVEHVVKAGNNAFEAIREITKGDFCDSVIDATGSLT 238 Query: 241 SMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIRDG 300 ++ F + HGG VGL KG I FS P+FH RE T+ SRNATREDF++V+ A+ G Sbjct: 239 AINQGFQYIAHGGNYVLVGLQKGEIAFSHPEFHKREATLMSSRNATREDFDQVLDAMASG 298 Query: 301 YIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVEL 338 VD YI+HR F ++ F+SWL PE+ VIKALVEL Sbjct: 299 QFSVDKYITHRVRFDQVKNDFQSWLNPETGVIKALVEL 336 >A5FCD7.1 A5FCD7_FLAJ1 Alcohol dehydrogenase, zinc-binding domain protein Length = 356 Score = 359 bits (921), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 172/341 (50%), Positives = 235/341 (68%), Gaps = 6/341 (1%) Query: 1 MKGIICEEVGKFVFRE-DLPEPELRDGEAIISIRRIGICGTDLHAYRGNQPYFTYPRVLG 59 MK I+CE+ +F +E ++PEP ++GE ++ I+RIGICGTD+HA+ G QPYF YPR+LG Sbjct: 20 MKFIVCEKPQEFKLKEKEIPEP--KEGEVLLKIKRIGICGTDIHAFGGTQPYFEYPRILG 77 Query: 60 HEVSGIIERIGDNPQGLRAGDQVSVIPYLHCGECRACLSGKTNCCQKMRVFGVHLDGGMR 119 HE++ E + N G + GD+V+ IPY +CG C AC +G TNCC ++VFGVH+DGGM Sbjct: 78 HELAA--EYVKGNAAGFKPGDKVTFIPYFNCGTCVACRNGLTNCCVNIKVFGVHIDGGMA 135 Query: 120 ERVSLPVRNLLKTDGLTLDQAAMLEPLAIGAHAVRRSGISAGDQVLVIGAGPIGLGVMAM 179 E VS+P + LL GL D+ A++EPLAI AH VRR+ + + D VLV+GAGPIG+G++ Sbjct: 136 EYVSIPEQYLLHGQGLDYDELALVEPLAIAAHGVRRAAVKSTDTVLVMGAGPIGIGLIQF 195 Query: 180 ARYAGGKVIAMDVNDERLVFCKGWAQAEHTVSALKEP-KEQLSALTDGNFLPHVIDSTGN 238 A+ AG KVI MD+ND RL FCK A+ T++ L + +L+ LT+G+ VID+TGN Sbjct: 196 AKIAGAKVIVMDINDYRLNFCKTELNADETINPLNDDVAAKLAELTNGDMANVVIDATGN 255 Query: 239 ISSMEGAFGLVGHGGTLTYVGLVKGNITFSDPDFHAREMTVQGSRNATREDFERVISAIR 298 M AF + HGG VGL KG ++FS PDFH RE T+ SRNAT EDFE V+ ++ Sbjct: 256 QKVMNSAFNYISHGGRFVLVGLQKGELSFSHPDFHKRESTLMSSRNATIEDFEYVMKCLK 315 Query: 299 DGYIDVDSYISHRCSFGEMIGQFESWLKPESKVIKALVELN 339 +G ID YI+HR SF EMI F + P++ VIKAL+E++ Sbjct: 316 EGKIDPKKYITHRTSFSEMITDFGQLIDPKNNVIKALIEID 356 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.140 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,150,872,808 Number of extensions: 94077392 Number of successful extensions: 311747 Number of sequences better than 1.0e-05: 9173 Number of HSP's gapped: 296651 Number of HSP's successfully gapped: 9234 Length of query: 339 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 203 Effective length of database: 1,779,438,163 Effective search space: 361225947089 Effective search space used: 361225947089 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)