BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0101.p (798 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFP4.1 B3KFP4_9BACI Pullulanase, type I 874 0.0 Q0TS57.1 Q0TS57_CLOP1 Pullulanase family protein 729 0.0 B1R580.1 B1R580_CLOPE Pullulanase family protein 724 0.0 B1RCQ4.1 B1RCQ4_CLOPE Pullulanase family protein 723 0.0 B1RMF6.1 B1RMF6_CLOPE Pullulanase family protein 720 0.0 >B3KFP4.1 B3KFP4_9BACI Pullulanase, type I Length = 784 Score = 874 bits (2259), Expect = 0.0, Method: Compositional matrix adjust. Identities = 438/781 (56%), Positives = 545/781 (69%), Gaps = 7/781 (0%) Query: 12 IEIYTGKDLGLTYTADYSLFKVWVPTAFTVSLVLYQTDGN-NIEGMVSGYRDSGRIVNMQ 70 I+ T LG TY + + F+VW P A V L LY+ G N +G+V+ + + G + M Sbjct: 2 IKSCTSVKLGATYRREGTAFQVWAPDAVNVKLALYEGPGEYNEQGLVTDH-EGGTLYAMV 60 Query: 71 RQEGGIWQARLHEDLKGMYYMYRAVFEDGTLNEAADPYAKAVSANGVRSAIVDLQDTDPE 130 R E G+W A+++ DL G YYMYR +G + A DPYA AVSANG R A+VDL ++P Sbjct: 61 RNEDGVWSAQVNRDLHGTYYMYRIESAEGGVCYAVDPYATAVSANGCRGAVVDLAKSNPP 120 Query: 131 GWDRDVSPALPHPADAVIYELHVRDFSAAADSGMRYKGKFKAFTEFGLKNSAGQAIGVDH 190 GW++DV P L P D+V+YELHVRDFS DSGM+Y+GK+KAFTE GL++S G A+G+DH Sbjct: 121 GWEQDVRPNLLKPTDSVLYELHVRDFSIHTDSGMKYRGKYKAFTEGGLRDSEGNALGLDH 180 Query: 191 LAELGITHVHLMPVADFQTVNEL-GVNSERSAEPFFTEYNWGYDPQHYNVPEGSYSTNPS 249 L ELG+THVHL+PV DF+TVNEL G S R + EYNWGYDPQ+YNVPEGSY+T+P Sbjct: 181 LEELGVTHVHLLPVFDFRTVNELDGYASGRLSR----EYNWGYDPQNYNVPEGSYATDPM 236 Query: 250 DPGTRIREMKEMVQALHSKGISVIMDVVYNHTYSVEKGPFQPLVPDYFYRHDYTGRLSNG 309 DP RIRE+KEMV ALH++GI V+MDVVYNHTY+V+ GPF+ L P YFYR D G LSNG Sbjct: 237 DPIVRIRELKEMVLALHARGIRVVMDVVYNHTYTVDDGPFERLAPGYFYRKDGYGNLSNG 296 Query: 310 SGVGNELATERPMVRKYIKDSLAYWASEYHIDGFRFDLMGLMDSVTMREIAEELRLEVNS 369 SGVGNELATE+PMVRKYIKDSL YWA EYHIDGFRFDLM L+D+ TM+EI ELR EV+ Sbjct: 297 SGVGNELATEKPMVRKYIKDSLKYWAEEYHIDGFRFDLMALIDTTTMKEIVSELRREVDR 356 Query: 370 DLLLYGEPWTGGDSPLATKTLKGVQKGKGYAVFNDNFRSAVKGDSDGWGKGFATGEYGKE 429 LL YGEPWTGG SPL +T+KG QKG+G+AVFND+FR A+KGD+DG G+GFATGE E Sbjct: 357 SLLFYGEPWTGGMSPLTEQTVKGSQKGQGFAVFNDHFRHAIKGDNDGRGRGFATGEPWYE 416 Query: 430 GAVASGIKGAIHEFTDSPVETVNYVTAHDNLNLWDKVLATQGLRQAARLPELENGRLKYG 489 GAV G+ G+IH F P ETVNYVT HDNLNLWDK+L QG+ + A L L +G+L Sbjct: 417 GAVVEGMMGSIHNFALHPEETVNYVTVHDNLNLWDKILVAQGMEEQAGLLRLTDGKLVNE 476 Query: 490 GSVEAAVAAADPYIGMDPDDILGNETVRRTLLANGIILTSQGIPFLQAGDELLRSKYGDH 549 G V A AA+ PY G+ +D++ VRR+LLANGI+L SQGIP L AGDELLR+KYGDH Sbjct: 477 GDVWQATAASTPYAGIPDEDVMSAAAVRRSLLANGIVLLSQGIPLLHAGDELLRTKYGDH 536 Query: 550 NSYRSPDAVNAIRWENKGKFLPVFEYYKGLIALRSGHPAFRLHGRQEIERSLEFLRCDSG 609 NSYRS DA+NAIRW NK +F PVF+YY+GLIALR HPAFR+ R+EIE +E LR Sbjct: 537 NSYRSGDAINAIRWSNKRRFKPVFDYYRGLIALRKAHPAFRMASREEIEGHMEVLRSSDR 596 Query: 610 VVAYLLKNNAGGDVWNNIVVIFNANPGPVSQRLPETDSCWNIVVDHTAARTDAFRSVQDP 669 ++AY L A W IVVI N N + LP T WNIVV+ A TD +++ Sbjct: 597 IIAYRLTKPAEQGSWGQIVVIVNGNEHEMPVDLPPTPYRWNIVVNDRQAGTDTIQTLDQG 656 Query: 670 EVQIEGLSMMVLYDKYGQPEPRSKVIEIRYERPDGDYRGWNLWVWGTGIQDGQHDFRQME 729 + I GLS+MVLY+ + +EI YER D Y GWN+WVWGTG+QDG F + + Sbjct: 657 KAVIPGLSLMVLYEDLAHMKQGLTAVEIEYERRDQAYEGWNVWVWGTGVQDGSIPFSRTD 716 Query: 730 DGGAVARIEVLPETSSVGYILRLNDWEEKDSAGDRFIDCSGSREGVKVTIRDREQENSGD 789 G A A T +G I+RLN+WE+++ D FID V++T+R + E+S Sbjct: 717 SGRARAVFYAASGTDRIGCIVRLNEWEDREGENDWFIDIPTDEAEVRITLRSGQNEDSDT 776 Query: 790 K 790 K Sbjct: 777 K 777 >Q0TS57.1 Q0TS57_CLOP1 Pullulanase family protein Length = 2638 Score = 729 bits (1881), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/690 (52%), Positives = 462/690 (66%), Gaps = 21/690 (3%) Query: 14 IYTGKDLGLTYTADYSLFKVWVPTAFTVSLVLYQTDGNNIEGMVSGYRDSGRIVNMQRQE 73 Y G DLG+TY D S FK+W PTA VSLVLY +G E GR ++M+++ Sbjct: 753 FYNGDDLGVTYKKDSSTFKLWAPTAKEVSLVLYDNEGTYDENGKVTDNTGGREISMKKEN 812 Query: 74 GGIWQARLHEDLKGMYYMYRAVFEDGTLNEAADPYAKAVSANGVRSAIVDLQDTDPEGWD 133 G+W ++ EDL+G YYMY+ F DG N A DPYAKAVSANG R AI+D T+P W Sbjct: 813 KGVWSLKVDEDLEGKYYMYKVSFSDGKTNYAIDPYAKAVSANGQRGAIIDFSSTNPSEWG 872 Query: 134 RDVSPALPHPADAVIYELHVRDFSAAADSGMRYKGKFKAFTEFGLKNSAGQA-IGVDHLA 192 P + +P D+++YE+HVRDFS + DSG+ KGKF+ E G K G+DHL Sbjct: 873 SVKKPPMLNPTDSILYEMHVRDFSISKDSGINNKGKFEGIAEEGTKVPGTDVKTGIDHLK 932 Query: 193 ELGITHVHLMPVADFQTVNELGVNSERSAEPFFTEYNWGYDPQHYNVPEGSYSTNPSDPG 252 ELGIT VHL+P D+ +VNE+ S +P ++NWGYDPQ+YN EGSYSTN DP Sbjct: 933 ELGITTVHLLPSFDYASVNEI------SNKP---QFNWGYDPQNYNSLEGSYSTNAEDPK 983 Query: 253 TRIREMKEMVQALHSKGISVIMDVVYNHTYSV-EKGPFQPLVPDYFYRHDYTGRLSNGSG 311 +R+RE K+MVQ LH GI V+MDVVYNHTY + + PF P+VP YFYR + G+ +NGSG Sbjct: 984 SRVREFKDMVQTLHKNGIRVVMDVVYNHTYGIGQNSPFDPVVPGYFYRTNDEGKYTNGSG 1043 Query: 312 VGNELATERPMVRKYIKDSLAYWASEYHIDGFRFDLMGLMDSVTMREIAEELRLEVNSDL 371 GNE+ATERPMVRKYIKDS+ Y A EY +DGFRFDLMGL+D+ TM E+ ELR E++ L Sbjct: 1044 CGNEVATERPMVRKYIKDSVKYLAKEYDVDGFRFDLMGLIDTKTMSELTTELRNEIDPSL 1103 Query: 372 LLYGEPWTGGDS--PLATKTLKGVQKGKGYAVFNDNFRSAVKGDSDGWGKGFATGEYGKE 429 ++YGEPW G S P +TLKG QKGKG+AVFNDNFR+A+KGDSDG G+GFATG GKE Sbjct: 1104 IIYGEPWQAGGSALPQEQQTLKGSQKGKGFAVFNDNFRNAIKGDSDGAGQGFATGASGKE 1163 Query: 430 GAVASGIKGAIHEFTDSPVETVNYVTAHDNLNLWDKVLATQGLRQAARLPELENGRL--- 486 GA+ +G+KG++ +FTDSP ET+NYVTAHDNLNLWDK++ G +AA E+ +G L Sbjct: 1164 GAIVAGVKGSVDDFTDSPSETINYVTAHDNLNLWDKIIKAAGKEKAAGFLEMRDGVLTGE 1223 Query: 487 ---KYGGSVEAAVAAADPYIGMDPDDILGNETVRRTLLANGIILTSQGIPFLQAGDELLR 543 KY EA A PY + + L NE VRR++L I +TSQGIPF QAGDE LR Sbjct: 1224 DANKYSSVQEAVNKKAKPYSVITETNPLDNEYVRRSILTTAITITSQGIPFFQAGDEFLR 1283 Query: 544 SKYGDHNSYRSPDAVNAIRWENKGKFLPVFEYYKGLIALRSGHPAFRLHGRQEIERSLEF 603 SKYGDHNSY+SPDA+N I W+NK KF VF+YY+GLI+LR HPAFR+ + IE +L Sbjct: 1284 SKYGDHNSYKSPDAINKINWQNKEKFSGVFDYYQGLISLRKSHPAFRMDTKAAIESNLVV 1343 Query: 604 LRCDSGVVAYLLKNNAGGDVWNNIVVIFNANPGPVSQRLPETDSCWNIVVDHTAARTDAF 663 + + +V + LKN A GD W NIVV +N N LP + WN+VV+ A T Sbjct: 1344 TKANDNIVVFQLKNFANGDSWKNIVVAYNGNNAEKEISLPH-EGDWNVVVNEHKAGTSVL 1402 Query: 664 RSVQD-PEVQIEGLSMMVLYDKYGQPEPRS 692 S++ V++ LS VLYD+ + E ++ Sbjct: 1403 ESLKGVNSVKVAPLSAKVLYDESVEGEAQT 1432 Score = 88.2 bits (217), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 40/90 (44%), Positives = 57/90 (63%), Gaps = 2/90 (2%) Query: 693 KVIEIRYERPDGDYRGWNLWVWGTGIQDGQHDFRQMEDGGAVARIEVLPETSSVGYILRL 752 K IE Y R D +Y GWN+W W TGI DGQ +F Q++DG A+++ E+ P+ + VG++LR Sbjct: 1604 KYIEFTYVRDDKNYDGWNIWTWQTGINDGQQNFTQIKDGKAISKFEIAPDVTQVGFVLRK 1663 Query: 753 -NDWEEKDS-AGDRFIDCSGSREGVKVTIR 780 W+EKD DR+I S KVT++ Sbjct: 1664 GTGWDEKDPYESDRYIQIDPSMNVTKVTVK 1693 >B1R580.1 B1R580_CLOPE Pullulanase family protein Length = 2643 Score = 724 bits (1868), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/690 (51%), Positives = 463/690 (67%), Gaps = 21/690 (3%) Query: 14 IYTGKDLGLTYTADYSLFKVWVPTAFTVSLVLYQTDGNNIEGMVSGYRDSGRIVNMQRQE 73 Y G DLG+TY D S FK+W PTA VSLVLY +G E GR ++M++++ Sbjct: 758 FYNGDDLGVTYKKDSSTFKLWAPTAKEVSLVLYDNEGTYDENGKVTDNTGGREISMKKED 817 Query: 74 GGIWQARLHEDLKGMYYMYRAVFEDGTLNEAADPYAKAVSANGVRSAIVDLQDTDPEGWD 133 G+W ++ E+L+G YYMY+ F DG N A DPYAKAVSANG R AI+D T+P W Sbjct: 818 KGVWSLKVDENLEGKYYMYKVSFSDGKTNYAIDPYAKAVSANGQRGAIIDFSSTNPSEWG 877 Query: 134 RDVSPALPHPADAVIYELHVRDFSAAADSGMRYKGKFKAFTEFGLKNSAGQA-IGVDHLA 192 P + +P D+++YE+HVRDFS + DSG++ KGKF+ E G K G+DHL Sbjct: 878 SVKKPPMLNPTDSILYEMHVRDFSISKDSGIKNKGKFEGIAEEGTKVPGTDVKTGIDHLK 937 Query: 193 ELGITHVHLMPVADFQTVNELGVNSERSAEPFFTEYNWGYDPQHYNVPEGSYSTNPSDPG 252 ELGIT VHL+P D+ +VNE+ S +P ++NWGYDPQ+YN EGSYSTN DP Sbjct: 938 ELGITTVHLLPSFDYASVNEI------SNKP---QFNWGYDPQNYNSLEGSYSTNTEDPK 988 Query: 253 TRIREMKEMVQALHSKGISVIMDVVYNHTYSV-EKGPFQPLVPDYFYRHDYTGRLSNGSG 311 +R+RE K+MVQ LH GI V+MDVVYNHTY + + PF P+VP YFYR + G+ +NGSG Sbjct: 989 SRVREFKDMVQTLHKNGIRVVMDVVYNHTYGIGQNSPFDPVVPGYFYRTNDEGKYTNGSG 1048 Query: 312 VGNELATERPMVRKYIKDSLAYWASEYHIDGFRFDLMGLMDSVTMREIAEELRLEVNSDL 371 GNE+ATERPMVRKYIKDS+ Y A EY +DGFRFDLMGL+D+ TM E+ ELR E++ L Sbjct: 1049 CGNEVATERPMVRKYIKDSVKYLAKEYDVDGFRFDLMGLIDTKTMSELTTELRNEIDPSL 1108 Query: 372 LLYGEPWTGGDS--PLATKTLKGVQKGKGYAVFNDNFRSAVKGDSDGWGKGFATGEYGKE 429 ++YGEPW G S P +TLKG QKGKG+AVFNDNFR+A+KGDSDG G+GFATG GKE Sbjct: 1109 IIYGEPWQAGGSALPQEQQTLKGSQKGKGFAVFNDNFRNAIKGDSDGAGQGFATGASGKE 1168 Query: 430 GAVASGIKGAIHEFTDSPVETVNYVTAHDNLNLWDKVLATQGLRQAARLPELENGRL--- 486 GA+ +G+KG++ +FTDSP ET+NYVTAHDNLNLWDK++ G +AA E+ +G L Sbjct: 1169 GAIVAGVKGSVDDFTDSPSETINYVTAHDNLNLWDKIIKAAGKEKAAGFLEMRDGVLTGE 1228 Query: 487 ---KYGGSVEAAVAAADPYIGMDPDDILGNETVRRTLLANGIILTSQGIPFLQAGDELLR 543 Y EA A PY + + L NE VRR++L I +TSQGIPF Q+GDE LR Sbjct: 1229 DANNYSSVQEAVNKKAKPYSLITETNPLDNEYVRRSILTTAITMTSQGIPFFQSGDEFLR 1288 Query: 544 SKYGDHNSYRSPDAVNAIRWENKGKFLPVFEYYKGLIALRSGHPAFRLHGRQEIERSLEF 603 SKYGDHNSY+SPDA+N I W+NK KF VF+YY+GLI+LR HPAFR+ + IE +L Sbjct: 1289 SKYGDHNSYKSPDAINQINWQNKEKFSGVFDYYQGLISLRKSHPAFRMDTKAAIESNLVV 1348 Query: 604 LRCDSGVVAYLLKNNAGGDVWNNIVVIFNANPGPVSQRLPETDSCWNIVVDHTAARTDAF 663 + + +V + LKN A GD W NIV+ +N N LP + WN+VV+ A T Sbjct: 1349 TKANDNIVVFQLKNFANGDSWKNIVLAYNGNNAEKEISLPH-EGDWNVVVNEHKAGTSVL 1407 Query: 664 RSVQD-PEVQIEGLSMMVLYDKYGQPEPRS 692 S++ V++ LS VLYD+ + E ++ Sbjct: 1408 ESLKGVNSVKVAPLSAKVLYDESVEGEAQT 1437 Score = 88.2 bits (217), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 41/90 (45%), Positives = 57/90 (63%), Gaps = 2/90 (2%) Query: 693 KVIEIRYERPDGDYRGWNLWVWGTGIQDGQHDFRQMEDGGAVARIEVLPETSSVGYILRL 752 K IE Y R D +Y GWN+W W TGI DGQ +F Q++DG AV++ E+ P+ + VG++LR Sbjct: 1609 KYIEFTYVRDDKNYDGWNIWTWQTGINDGQQNFTQIKDGKAVSKFEIEPDVTQVGFVLRK 1668 Query: 753 -NDWEEKDS-AGDRFIDCSGSREGVKVTIR 780 W+EKD DR+I S KVT++ Sbjct: 1669 GTGWDEKDPYESDRYIQIDPSMNVTKVTVK 1698 >B1RCQ4.1 B1RCQ4_CLOPE Pullulanase family protein Length = 2639 Score = 723 bits (1866), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/690 (52%), Positives = 462/690 (66%), Gaps = 21/690 (3%) Query: 14 IYTGKDLGLTYTADYSLFKVWVPTAFTVSLVLYQTDGNNIEGMVSGYRDSGRIVNMQRQE 73 Y G DLG+TY D S FK+W PTA VSLVLY +G E GR ++M++++ Sbjct: 758 FYNGDDLGVTYKKDSSTFKLWAPTAKEVSLVLYDNEGTYDENGKVTDNTGGREISMKKED 817 Query: 74 GGIWQARLHEDLKGMYYMYRAVFEDGTLNEAADPYAKAVSANGVRSAIVDLQDTDPEGWD 133 G+W ++ E+L+G YYMY+ F DG N A DPYAKAVSANG R AI++ T+P W Sbjct: 818 KGVWSLKVDENLEGKYYMYKVSFADGKTNYAIDPYAKAVSANGQRGAIIEFSSTNPSEWG 877 Query: 134 RDVSPALPHPADAVIYELHVRDFSAAADSGMRYKGKFKAFTEFGLKNSAGQA-IGVDHLA 192 P + +P D+++YE+HVRDFS + DSG+ KGKF+ E G K G+DHL Sbjct: 878 SVKKPPMLNPTDSILYEMHVRDFSISKDSGINNKGKFEGIAEKGTKVPGTDVKTGIDHLK 937 Query: 193 ELGITHVHLMPVADFQTVNELGVNSERSAEPFFTEYNWGYDPQHYNVPEGSYSTNPSDPG 252 ELGIT VHL+P D+ +VNE+ S +P ++NWGYDPQ+YN EGSYSTN DP Sbjct: 938 ELGITTVHLLPSFDYASVNEI------SNKP---QFNWGYDPQNYNSLEGSYSTNAEDPK 988 Query: 253 TRIREMKEMVQALHSKGISVIMDVVYNHTYSV-EKGPFQPLVPDYFYRHDYTGRLSNGSG 311 +R+RE K+MVQALH GI V+MDVVYNHTY + + PF P+VP YFYR + G+ +NGSG Sbjct: 989 SRVREFKDMVQALHKNGIRVVMDVVYNHTYGIGQNSPFDPVVPGYFYRTNDEGKYTNGSG 1048 Query: 312 VGNELATERPMVRKYIKDSLAYWASEYHIDGFRFDLMGLMDSVTMREIAEELRLEVNSDL 371 GNE+ATERPMVRKYIKDS+ Y A EY +DGFRFDLMGL+D+ TM E+ ELR E++ L Sbjct: 1049 CGNEVATERPMVRKYIKDSVKYLAKEYDVDGFRFDLMGLIDTKTMSELTTELRNEIDPSL 1108 Query: 372 LLYGEPWTGGDS--PLATKTLKGVQKGKGYAVFNDNFRSAVKGDSDGWGKGFATGEYGKE 429 ++YGEPW G S P +TLKG QKGKG+AVFNDNFR+A+KGDSDG G+GFATG KE Sbjct: 1109 IIYGEPWQAGGSALPQEQQTLKGSQKGKGFAVFNDNFRNAIKGDSDGAGQGFATGASSKE 1168 Query: 430 GAVASGIKGAIHEFTDSPVETVNYVTAHDNLNLWDKVLATQGLRQAARLPELENGRL--- 486 G + +G+KG++ +FTDSP ET+NYVTAHDNLNLWDK++ G +AA E+ +G L Sbjct: 1169 GDIVAGVKGSVDDFTDSPSETINYVTAHDNLNLWDKIIKAAGKEKAAGFLEMRDGVLTGE 1228 Query: 487 ---KYGGSVEAAVAAADPYIGMDPDDILGNETVRRTLLANGIILTSQGIPFLQAGDELLR 543 KY EA A PY + + L NE VRR++L I +TSQGIPF QAGDE LR Sbjct: 1229 DANKYSSVQEAVNKKAKPYSVITETNPLDNEYVRRSILTTAITMTSQGIPFFQAGDEFLR 1288 Query: 544 SKYGDHNSYRSPDAVNAIRWENKGKFLPVFEYYKGLIALRSGHPAFRLHGRQEIERSLEF 603 SKYGDHNSY+SPDA+N I W+NK KF VF+YY+GLIALR HPAFR+ + IE +L Sbjct: 1289 SKYGDHNSYKSPDAINEINWQNKEKFSGVFDYYQGLIALRKSHPAFRMDTKAAIESNLVV 1348 Query: 604 LRCDSGVVAYLLKNNAGGDVWNNIVVIFNANPGPVSQRLPETDSCWNIVVDHTAARTDAF 663 + + +V + LKN A GD W NIVV +N N LP + WN++V+ A T Sbjct: 1349 TKANDNIVVFQLKNFANGDSWKNIVVAYNGNNAEKEISLPH-EGDWNVIVNEHKAGTSVL 1407 Query: 664 RSVQD-PEVQIEGLSMMVLYDKYGQPEPRS 692 S++ V++ LS VLYD+ + E ++ Sbjct: 1408 ESLKGVNSVKVAPLSAKVLYDESVEGEEQT 1437 Score = 87.8 bits (216), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 40/90 (44%), Positives = 57/90 (63%), Gaps = 2/90 (2%) Query: 693 KVIEIRYERPDGDYRGWNLWVWGTGIQDGQHDFRQMEDGGAVARIEVLPETSSVGYILRL 752 K IE Y R D +Y GWN+W W TG+ DGQ +F Q++DG AV++ E+ P+ + VG++LR Sbjct: 1609 KYIEFTYVRDDKNYDGWNIWTWQTGVNDGQQNFTQIKDGKAVSKFEIAPDVTQVGFVLRK 1668 Query: 753 -NDWEEKDS-AGDRFIDCSGSREGVKVTIR 780 W+EKD DR+I S KVT++ Sbjct: 1669 GTGWDEKDPYESDRYIKIDPSMNVTKVTVK 1698 >B1RMF6.1 B1RMF6_CLOPE Pullulanase family protein Length = 2639 Score = 720 bits (1859), Expect = 0.0, Method: Compositional matrix adjust. Identities = 357/690 (51%), Positives = 460/690 (66%), Gaps = 21/690 (3%) Query: 14 IYTGKDLGLTYTADYSLFKVWVPTAFTVSLVLYQTDGNNIEGMVSGYRDSGRIVNMQRQE 73 Y G DLG+TY D S FK+W PTA VSLVLY +G E GR ++M++++ Sbjct: 758 FYNGDDLGVTYKKDSSTFKLWAPTAKEVSLVLYDNEGTYDENGKVTDNTGGREISMKKED 817 Query: 74 GGIWQARLHEDLKGMYYMYRAVFEDGTLNEAADPYAKAVSANGVRSAIVDLQDTDPEGWD 133 G+W ++ E+L+G YYMY+ F DG N A DPYAKAVSANG R I+D T+P W Sbjct: 818 KGVWSLKVDENLEGKYYMYKVSFADGKTNYAIDPYAKAVSANGQRGVIIDFSSTNPSEWG 877 Query: 134 RDVSPALPHPADAVIYELHVRDFSAAADSGMRYKGKFKAFTEFGLKNSAGQA-IGVDHLA 192 P + +P D+++YE+HVRDFS + DSG++ KGKF+ E G K G+DHL Sbjct: 878 SVKKPPMLNPTDSILYEMHVRDFSISQDSGIKNKGKFEGIAEEGTKVPGTDVKTGIDHLK 937 Query: 193 ELGITHVHLMPVADFQTVNELGVNSERSAEPFFTEYNWGYDPQHYNVPEGSYSTNPSDPG 252 ELGIT VHL+P D+ +VNE+ S +P ++NWGYDPQ+YN EGSYSTN DP Sbjct: 938 ELGITTVHLLPSFDYASVNEI------SNKP---QFNWGYDPQNYNSLEGSYSTNTEDPK 988 Query: 253 TRIREMKEMVQALHSKGISVIMDVVYNHTYSV-EKGPFQPLVPDYFYRHDYTGRLSNGSG 311 +R+RE K+MVQ LH GI V+MDVVYNHTY + + PF P+VP YFYR + G+ +NGSG Sbjct: 989 SRVREFKDMVQTLHKNGIRVVMDVVYNHTYGIGQNSPFDPVVPGYFYRTNDEGKYTNGSG 1048 Query: 312 VGNELATERPMVRKYIKDSLAYWASEYHIDGFRFDLMGLMDSVTMREIAEELRLEVNSDL 371 GNE+ATERPMVRKYIKDS+ Y A EY +DGFRFDLMGL+D+ TM E+ ELR E++ L Sbjct: 1049 CGNEVATERPMVRKYIKDSVKYLAKEYDVDGFRFDLMGLIDTKTMSELTTELRNEIDPSL 1108 Query: 372 LLYGEPWTGGDS--PLATKTLKGVQKGKGYAVFNDNFRSAVKGDSDGWGKGFATGEYGKE 429 ++YGEPW G S P +TLKG QKGKG+AVFNDNFR+A+KGDSDG G+GFATG KE Sbjct: 1109 IIYGEPWQAGGSALPQEQQTLKGSQKGKGFAVFNDNFRNAIKGDSDGAGQGFATGASSKE 1168 Query: 430 GAVASGIKGAIHEFTDSPVETVNYVTAHDNLNLWDKVLATQGLRQAARLPELENGRL--- 486 G + +G+KG++ +FTDSP ET+NYVTAHDNLNLWDK++ G +AA E+ +G L Sbjct: 1169 GDIVAGVKGSVDDFTDSPSETINYVTAHDNLNLWDKIIKAAGKEKAAGFLEMRDGVLTGE 1228 Query: 487 ---KYGGSVEAAVAAADPYIGMDPDDILGNETVRRTLLANGIILTSQGIPFLQAGDELLR 543 Y EA A PY + + L NE VRR++L I +TSQGIPF QAGDE LR Sbjct: 1229 DANNYSSVQEAVEKKAKPYSVITETNPLDNEYVRRSILTTAITMTSQGIPFFQAGDEFLR 1288 Query: 544 SKYGDHNSYRSPDAVNAIRWENKGKFLPVFEYYKGLIALRSGHPAFRLHGRQEIERSLEF 603 SKYGDHNSY+SPDA+N I W+NK KF VF+YY+GLI+LR HPAFR+ + IE +L Sbjct: 1289 SKYGDHNSYKSPDAINEINWQNKEKFSGVFDYYQGLISLRKSHPAFRMDTKAAIESNLVV 1348 Query: 604 LRCDSGVVAYLLKNNAGGDVWNNIVVIFNANPGPVSQRLPETDSCWNIVVDHTAARTDAF 663 + + +V + LKN A GD W NIVV +N N LP + WN+VV+ A T Sbjct: 1349 TKANDNIVVFQLKNFANGDSWKNIVVAYNGNNAEKEISLPH-EGDWNVVVNEHKAGTSVL 1407 Query: 664 RSVQD-PEVQIEGLSMMVLYDKYGQPEPRS 692 S++ V++ LS VLYD+ + E ++ Sbjct: 1408 ESLKGVNSVKVAPLSAKVLYDESVEGEEQT 1437 Score = 87.4 bits (215), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 39/90 (43%), Positives = 57/90 (63%), Gaps = 2/90 (2%) Query: 693 KVIEIRYERPDGDYRGWNLWVWGTGIQDGQHDFRQMEDGGAVARIEVLPETSSVGYILRL 752 K IE Y R D +Y GWN+W W TG+ DGQ +F Q++DG A+++ E+ P+ + VG++LR Sbjct: 1609 KYIEFTYVRDDKNYDGWNIWTWQTGVNDGQQNFTQIKDGKAISKFEIAPDVTQVGFVLRK 1668 Query: 753 -NDWEEKDS-AGDRFIDCSGSREGVKVTIR 780 W+EKD DR+I S KVT++ Sbjct: 1669 GTGWDEKDPYESDRYIKIDPSMNVTKVTVK 1698 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 5,272,664,128 Number of extensions: 242521831 Number of successful extensions: 575139 Number of sequences better than 1.0e-05: 2526 Number of HSP's gapped: 565424 Number of HSP's successfully gapped: 2942 Length of query: 798 Length of database: 3,060,696,203 Length adjustment: 144 Effective length of query: 654 Effective length of database: 1,704,070,043 Effective search space: 1114461808122 Effective search space used: 1114461808122 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 136 (57.0 bits)