BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0100.p (544 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4L089.1 C4L089_EXISA Diguanylate cyclase with PAS/PAC sensor 197 2e-48 B1HMF0.1 B1HMF0_LYSSC Hypothetical yegE protein 165 1e-38 A3IFN9.1 A3IFN9_9BACI Putative uncharacterized protein (Fragment) 163 4e-38 Q2W154.1 Q2W154_MAGMM Putative uncharacterized protein 163 5e-38 B8GV15.1 B8GV15_THISH Diguanylate cyclase with PAS/PAC sensor 162 7e-38 >C4L089.1 C4L089_EXISA Diguanylate cyclase with PAS/PAC sensor Length = 686 Score = 197 bits (502), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 111/285 (38%), Positives = 169/285 (59%), Gaps = 8/285 (2%) Query: 251 YQLIGETMSDFIILTDSNGQILYASPSHSSLLGYLPG--RGTPLTNYIREAEITWVK--L 306 Y+LI E MSD + + + +G + YASPSH ++LG+ G G +YI +I V+ L Sbjct: 400 YRLITENMSDLVCILERDGTVRYASPSHRTVLGFAAGYYEGKNTLDYIHPDDIHIVRGQL 459 Query: 307 KSVVQSTPRIS-ELRMRAGDGHWVWLETKVTPIKGGGAVPAQFMLVSREITERKQHEERL 365 K + + ++ E R D W+WLETK+ I ++ VSREI +RK E++L Sbjct: 460 KEMKEQDVSLTLEFRHFHADQRWIWLETKIQAIYDESGHLLHYLTVSREIMQRKALEKQL 519 Query: 366 HKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHLAGDE 425 LA++D LT +PNR F Y+E I + E + E+A+ LD DRFKQ+NDT GH GDE Sbjct: 520 KHLAYHDTLTDLPNRRFFLAYLEEAIDRTEFEQGELAVLALDVDRFKQINDTFGHDVGDE 579 Query: 426 FLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRLLQLFNKSWSVNKG 485 L+ L+R L ++ R+GGDEFAV++ RF P L + ++++Q + W +++ Sbjct: 580 LLRQLARRLTSVLRKQDMIARLGGDEFAVLI-RFNDPCALHRVAEKIIQALQRPWEIDQ- 637 Query: 486 SSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANL 530 +F T++S+G A Y + G SI +LL+ ADLA+Y +K G N+ L Sbjct: 638 HTFITTSSVGGARYVK-GLSIKQLLKRADLALYEAKAGGRNQYAL 681 >B1HMF0.1 B1HMF0_LYSSC Hypothetical yegE protein Length = 675 Score = 165 bits (417), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 102/291 (35%), Positives = 156/291 (53%), Gaps = 13/291 (4%) Query: 251 YQLIGETMSDFIILTDSNGQILYASPSHSSLLGY--LPGRGTPLTNYI-REAEITW-VKL 306 ++LI E +D I++T+ +G I+YASPS+ LGY + +G + I E++ W + Sbjct: 383 FRLITENTNDLIVITNEDGIIIYASPSYEIYLGYENVELQGQFYSAIIDTESQHEWQTFI 442 Query: 307 KSVVQSTPRISELRMRAGDGHWVWLETKVTPIKGGG-AVPAQFMLVSREITERKQHEERL 365 + T EL ++A DG +W E VT + +Q M+VSREIT RK+ E L Sbjct: 443 NNYTGQTDTQFELLLKAKDGTPIWTEGNVTVVHDPDREKVSQIMMVSREITHRKERENDL 502 Query: 366 HKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHLAGDE 425 LA++D LT +PNR + + ++++ E IA+ LD D FK +ND GH GD Sbjct: 503 LYLAYHDSLTQLPNRRYLQKEFPTILAEANENNASIAMLYLDGDDFKAVNDRFGHDTGDA 562 Query: 426 FLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASP-----EMLDEILDRLLQLFNKSW 480 F+Q + L +TV+ RIGGDEF V+L + I+ R+ W Sbjct: 563 FIQSFGQALSKTVRSHDIVIRIGGDEFLVILTGLTREIEKRHSQIMHIIRRIRDELATGW 622 Query: 481 SVNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLY 531 + + F +ASIG++ YP H +++EL+ AD A+Y+SK+ G N NLY Sbjct: 623 MI-ENHQFTPTASIGISYYPDHAQTLDELMDLADQALYKSKEAGKN--NLY 670 >A3IFN9.1 A3IFN9_9BACI Putative uncharacterized protein (Fragment) Length = 610 Score = 163 bits (412), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 99/286 (34%), Positives = 152/286 (53%), Gaps = 11/286 (3%) Query: 251 YQLIGETMSDFIILTDSNGQILYASPSHSSLLGYLPG--RGTPLTNYI-REAEITW-VKL 306 ++LI E +D I++T+ +G I+Y SPS+ LGY G +G + I E++ W L Sbjct: 318 FRLITENTNDLIVITNEDGIIIYTSPSYEIYLGYENGELQGQFYSAIIDTESQHEWQTFL 377 Query: 307 KSVVQSTPRISELRMRAGDGHWVWLETKVTPIKGGG-AVPAQFMLVSREITERKQHEERL 365 + T EL ++A DG +W E VT + +Q M+VSREIT RK+ E L Sbjct: 378 NNFTGQTDTQFELLLKAKDGTPIWTEGNVTVVHDPDREKVSQIMMVSREITHRKERENDL 437 Query: 366 HKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHLAGDE 425 LA++D LT +PNR + + ++++ +E +A+ LD D FK +ND GH GD Sbjct: 438 LYLAYHDSLTQLPNRRYLQNEFPTILAEADENNASVAMLYLDGDDFKAVNDRFGHDTGDA 497 Query: 426 FLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASP-----EMLDEILDRLLQLFNKSW 480 F+Q L +TV+ RIGGDEF V+L + I+ R+ W Sbjct: 498 FIQSFGHALSKTVRSHDIVIRIGGDEFLVILTGLTREIDKRHSQIMHIIRRIRDELAVGW 557 Query: 481 SVNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGN 526 + + F +ASIG++ YP H +++EL+ AD A+Y+SK+ G N Sbjct: 558 MI-EDHQFTPTASIGISYYPDHAQTLDELMDLADQALYKSKEAGKN 602 >Q2W154.1 Q2W154_MAGMM Putative uncharacterized protein Length = 802 Score = 163 bits (412), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 101/294 (34%), Positives = 156/294 (53%), Gaps = 18/294 (6%) Query: 262 IILTDSNGQILYASPSHSSLLGY----LPGR-------GTPLTNYIREAEITWVKLKSVV 310 +++TDS+G I Y + + + GY + GR G + + R+ W L S Sbjct: 261 VMITDSHGAIEYVNRAFVLVSGYSEDEVLGRNPRLLKSGETVASLYRD---MWHCLAS-- 315 Query: 311 QSTPRISELRMRAGDGHWVWLETKVTPIKGGGAVPAQFMLVSREITERKQHEERLHKLAF 370 EL RA DG W ++PI+ V FM V IT+RK+ E++L A Sbjct: 316 -GATWQGELCNRAKDGSLFWEYATISPIRDDDGVVTHFMAVKENITQRKETEQQLVHQAT 374 Query: 371 YDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHLAGDEFLQLL 430 +D LT +PNR + + + I + + I L LD DRFK +ND+LGH+AGDE L+++ Sbjct: 375 HDTLTGLPNRLLLEDRVHHAIEVAKREGRRIGLMFLDLDRFKIVNDSLGHVAGDELLKVV 434 Query: 431 SRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRLLQLFNKSWSVNKGSSFHT 490 S L+ T++ + R+GGDEF VVL F S L E+ +++ ++ ++ G H Sbjct: 435 SDRLRHTLRRSDTVARLGGDEFVVVLTHFQSTTELAEVAEKISAALDEPVELS-GHKVHV 493 Query: 491 SASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLYSELVDEEYKDQPK 544 ASIG+A+YP G + N L++ AD AMYR+K+ G N Y +++E D+ K Sbjct: 494 GASIGIAIYPDDGDNFNALMKDADTAMYRAKQKGRNTFCFYDSNMNDEALDRLK 547 >B8GV15.1 B8GV15_THISH Diguanylate cyclase with PAS/PAC sensor Length = 450 Score = 162 bits (411), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 104/304 (34%), Positives = 157/304 (51%), Gaps = 9/304 (2%) Query: 235 RKLIDLQNEMLEEKSLYQLIGETMSDFIILTDSNGQILYASPSHSSLLGYL--PGRGTPL 292 RKL++ Q+ L E L+ E +D I G++ Y SP+ +LGY P G Sbjct: 146 RKLLE-QDLRLSEAKFRTLV-ENANDIIYTLTPEGELSYISPNWQDVLGYEVEPFIGHSY 203 Query: 293 TNYIREAEITWVK--LKSVVQSTPRIS--ELRMRAGDGHWVWLETKVTPIKGGGAVPAQF 348 I ++ + +++V + + S E R+R DG W W + +P+ + + Sbjct: 204 EPLIHPNDLDRTREFMRAVFTTGKKQSGVEYRIRHADGSWRWHTSNASPVLDEAGRVSAY 263 Query: 349 MLVSREITERKQHEERLHKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDC 408 + ++REITERKQ E ++H LA +D LT +PNRA F +E I + +++AL +D Sbjct: 264 LGIAREITERKQQEAQIHHLAHHDLLTGLPNRAMFFDRLEAAIDEASAESRKLALMFVDL 323 Query: 409 DRFKQLNDTLGHLAGDEFLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEI 468 DRFK +ND GH GD+ LQ ++ + ++ RIGGDEF V+LH + + Sbjct: 324 DRFKPVNDRHGHAVGDQLLQAVAGRMHTILRAGDLVGRIGGDEFIVLLHDIRTEDDALTA 383 Query: 469 LDRLLQLFNKSWSVNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEA 528 ++L + + +G SASIG+ALYP H + EL R AD AMY SK G N Sbjct: 384 AEKLRCHLETPFDI-EGRRLQISASIGIALYPSHADNARELARRADEAMYLSKAEGRNLT 442 Query: 529 NLYS 532 LYS Sbjct: 443 TLYS 446 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,973,939,255 Number of extensions: 116881633 Number of successful extensions: 366508 Number of sequences better than 1.0e-05: 14274 Number of HSP's gapped: 349947 Number of HSP's successfully gapped: 14305 Length of query: 544 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 403 Effective length of database: 1,732,333,088 Effective search space: 698130234464 Effective search space used: 698130234464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)