BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0098.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB33.1 B3KB33_9BACI Phenylalanyl-tRNA synthetase, alpha subunit 573 e-162 B1D7E7.1 B1D7E7_9BACL Phenylalanyl-tRNA synthetase, alpha subunit 535 e-150 C5D627.1 C5D627_9BACI Phenylalanyl-tRNA synthetase, alpha subunit 488 e-136 A7Z7F3.1 A7Z7F3_BACA2 PheS EC=6.1.1.20 486 e-135 Q659J3.1 Q659J3_BACAM Phenylalanyl-tRNA synthetase alpha chain E... 486 e-135 >B3KB33.1 B3KB33_9BACI Phenylalanyl-tRNA synthetase, alpha subunit Length = 332 Score = 573 bits (1477), Expect = e-162, Method: Compositional matrix adjust. Identities = 271/326 (83%), Positives = 294/326 (90%) Query: 19 VTDPQLLNDLRVKYLGKKGELTEVLRGMGGLSAEERPVIGQVANLVRSAIEEIISTKQEA 78 V DPQ LNDLRVKYLGKKG LTE+LRGMG LSAEERPVIGQVAN VR AIEE+IS KQEA Sbjct: 7 VNDPQQLNDLRVKYLGKKGALTEILRGMGALSAEERPVIGQVANDVRGAIEEVISGKQEA 66 Query: 79 FQQQETLNRLNAEKVDVTLPGRRLPQGGIHPVSRVVREIEDIFIGMGYRVAEGPEVETDY 138 F QQET RL AEKVDVTLPGR+L QG +HP+S+V+++IEDIFIGMGYRVAEGPEVETDY Sbjct: 67 FLQQETEERLRAEKVDVTLPGRKLSQGAVHPLSKVIQDIEDIFIGMGYRVAEGPEVETDY 126 Query: 139 YNFEALNLPKNHPARDMQDSFYLTEDILMRTQTSPVQIRTMQAMKGETPVKIICPGKVFR 198 +NFEALNLPKNHPARDMQDSFYLTED+LMRTQTSPVQ RTM +M+G+ PVKIICPG+VFR Sbjct: 127 FNFEALNLPKNHPARDMQDSFYLTEDLLMRTQTSPVQARTMLSMQGKAPVKIICPGRVFR 186 Query: 199 RDDDDATHSFQFHQIEGLVIGRHIRMSDLKGTLQQFVQEMFGPNTGIRLRPSFFPFTEPS 258 RDDDDATHSFQFHQIEGLVIG +IRMSDLKGTL QFVQEMFG NT IRLRPSFFPFTEPS Sbjct: 187 RDDDDATHSFQFHQIEGLVIGENIRMSDLKGTLLQFVQEMFGDNTQIRLRPSFFPFTEPS 246 Query: 259 VEVDVSCFKCGGHGCRLCKQSGWLEILGAGMVHPNVLEKGGYDPAEYSGFAFGMGVERIA 318 EVDVSC KCGG GCR+CKQ+GWLEILG GMVHP VLE GGYDP +YSGFAFGMGVERIA Sbjct: 247 AEVDVSCVKCGGSGCRVCKQTGWLEILGCGMVHPKVLEMGGYDPEKYSGFAFGMGVERIA 306 Query: 319 MLKYGIDDIRYFYTNDMAFVKQFKGI 344 MLKYG+DDIR+F+ ND AF+ QF I Sbjct: 307 MLKYGVDDIRHFFNNDTAFLNQFTRI 332 >B1D7E7.1 B1D7E7_9BACL Phenylalanyl-tRNA synthetase, alpha subunit Length = 343 Score = 535 bits (1378), Expect = e-150, Method: Compositional matrix adjust. Identities = 249/323 (77%), Positives = 282/323 (87%), Gaps = 1/323 (0%) Query: 19 VTDPQLLNDLRVKYLGKKGELTEVLRGMGGLSAEERPVIGQVANLVRSAIEEIISTKQEA 78 V +PQ LNDLRVKYLGKKG LTE+LRGMGGLSAEERPVIGQV N VR+AIE +I KQ A Sbjct: 19 VDNPQQLNDLRVKYLGKKGALTEILRGMGGLSAEERPVIGQVGNDVRAAIEGVIDEKQNA 78 Query: 79 FQQQETLNRLNAEKVDVTLPGRRLPQGGIHPVSRVVREIEDIFIGMGYRVAEGPEVETDY 138 F ET NRL AE +DVTLPG+ LP G +HP+++V +EIED+F+G+GY +AEGPEVETDY Sbjct: 79 FLAAETENRLRAETIDVTLPGKALPTGAVHPLNKVAQEIEDVFVGLGYSIAEGPEVETDY 138 Query: 139 YNFEALNLPKNHPARDMQDSFYLTEDILMRTQTSPVQIRTMQAMKGETPVKIICPGKVFR 198 +NFEALNLPKNHPARDMQDSFY+TEDILMRT TSPVQ+RTMQ M G+ P+K+ICPGKV+R Sbjct: 139 FNFEALNLPKNHPARDMQDSFYITEDILMRTHTSPVQVRTMQQMNGK-PIKVICPGKVYR 197 Query: 199 RDDDDATHSFQFHQIEGLVIGRHIRMSDLKGTLQQFVQEMFGPNTGIRLRPSFFPFTEPS 258 RDDDDATHSF F+QIEGLV+G +IRMSDLKGTL QFVQ+MFG IRLRPSFFPFTEPS Sbjct: 198 RDDDDATHSFMFNQIEGLVVGPNIRMSDLKGTLLQFVQQMFGEQAQIRLRPSFFPFTEPS 257 Query: 259 VEVDVSCFKCGGHGCRLCKQSGWLEILGAGMVHPNVLEKGGYDPAEYSGFAFGMGVERIA 318 EVDV+C +CGGHGCR+CKQ+GWLEILG GMVHP VLE GGYDP E SGFAFGMGVERIA Sbjct: 258 AEVDVTCVQCGGHGCRMCKQTGWLEILGCGMVHPRVLEMGGYDPKEVSGFAFGMGVERIA 317 Query: 319 MLKYGIDDIRYFYTNDMAFVKQF 341 +LKYGIDDIR+FYTND F+ QF Sbjct: 318 LLKYGIDDIRHFYTNDQRFLSQF 340 >C5D627.1 C5D627_9BACI Phenylalanyl-tRNA synthetase, alpha subunit Length = 344 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 226/323 (69%), Positives = 267/323 (82%) Query: 19 VTDPQLLNDLRVKYLGKKGELTEVLRGMGGLSAEERPVIGQVANLVRSAIEEIISTKQEA 78 D + LN++RV YLGKKG +TEVLRGMG LS EERPVIG +AN VR AI+ + KQ Sbjct: 19 ANDLKALNEVRVAYLGKKGPITEVLRGMGALSPEERPVIGALANEVRQAIQSALEAKQAK 78 Query: 79 FQQQETLNRLNAEKVDVTLPGRRLPQGGIHPVSRVVREIEDIFIGMGYRVAEGPEVETDY 138 +Q+E +L AE +DVTLPGR + +G HP++RV+ EIED+FIGMGY +AEGPEVE DY Sbjct: 79 LEQEEVEKKLAAEAIDVTLPGRPVKRGNHHPLTRVIEEIEDLFIGMGYTIAEGPEVEKDY 138 Query: 139 YNFEALNLPKNHPARDMQDSFYLTEDILMRTQTSPVQIRTMQAMKGETPVKIICPGKVFR 198 YNFEALNLPK HPARDMQDSFY+TE+IL+RT TSPVQ RTM+ KG PVKIICPGKV+R Sbjct: 139 YNFEALNLPKGHPARDMQDSFYITEEILLRTHTSPVQARTMEKHKGRGPVKIICPGKVYR 198 Query: 199 RDDDDATHSFQFHQIEGLVIGRHIRMSDLKGTLQQFVQEMFGPNTGIRLRPSFFPFTEPS 258 RD+DDATHS QF QIEGLV+ +IRMSDLKGTL++F ++MFG + IR RPSFFPFTEPS Sbjct: 199 RDNDDATHSHQFTQIEGLVVDENIRMSDLKGTLREFARKMFGKDREIRFRPSFFPFTEPS 258 Query: 259 VEVDVSCFKCGGHGCRLCKQSGWLEILGAGMVHPNVLEKGGYDPAEYSGFAFGMGVERIA 318 VEVDVSCF CGGHGC +CK +GW+EILGAGMVHPNVLE G+D +Y+GFAFGMG ERIA Sbjct: 259 VEVDVSCFHCGGHGCSVCKGTGWIEILGAGMVHPNVLEMAGFDSKKYTGFAFGMGPERIA 318 Query: 319 MLKYGIDDIRYFYTNDMAFVKQF 341 MLKYGIDDIR+FY ND+ F++QF Sbjct: 319 MLKYGIDDIRHFYQNDIRFLQQF 341 >A7Z7F3.1 A7Z7F3_BACA2 PheS EC=6.1.1.20 Length = 345 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 224/318 (70%), Positives = 268/318 (84%) Query: 25 LNDLRVKYLGKKGELTEVLRGMGGLSAEERPVIGQVANLVRSAIEEIISTKQEAFQQQET 84 +ND+RV+YLGKKG +TEVLRGMG LSAEERP +G +AN VR I I+ K E +Q+E Sbjct: 26 VNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIAAAITAKNEQLEQEEM 85 Query: 85 LNRLNAEKVDVTLPGRRLPQGGIHPVSRVVREIEDIFIGMGYRVAEGPEVETDYYNFEAL 144 +L+++ +DVTLPG ++ GG HP++ V+ EIED+FIGMGY V EGPEVETDYYNFEAL Sbjct: 86 NKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFEAL 145 Query: 145 NLPKNHPARDMQDSFYLTEDILMRTQTSPVQIRTMQAMKGETPVKIICPGKVFRRDDDDA 204 NLPK HPARDMQDSFY+TE++LMRTQTSPVQ RTM+ KG+ PVKIICPGKV+RRD+DDA Sbjct: 146 NLPKEHPARDMQDSFYITEEMLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDA 205 Query: 205 THSFQFHQIEGLVIGRHIRMSDLKGTLQQFVQEMFGPNTGIRLRPSFFPFTEPSVEVDVS 264 THS QF QIEGLV+ R I MSDLKGTL+ ++MFG + IRLRPSFFPFTEPSVEVDV+ Sbjct: 206 THSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMFGQDREIRLRPSFFPFTEPSVEVDVT 265 Query: 265 CFKCGGHGCRLCKQSGWLEILGAGMVHPNVLEKGGYDPAEYSGFAFGMGVERIAMLKYGI 324 CFKCGG GC +CK++GW+EILGAGMVHPNVL+ G++P EY GFAFGMGVERIAMLKYGI Sbjct: 266 CFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAGFNPEEYQGFAFGMGVERIAMLKYGI 325 Query: 325 DDIRYFYTNDMAFVKQFK 342 +DIR+FYTND+ F+ QFK Sbjct: 326 EDIRHFYTNDVRFISQFK 343 >Q659J3.1 Q659J3_BACAM Phenylalanyl-tRNA synthetase alpha chain EC=6.1.1.20 Length = 344 Score = 486 bits (1252), Expect = e-135, Method: Compositional matrix adjust. Identities = 224/318 (70%), Positives = 268/318 (84%) Query: 25 LNDLRVKYLGKKGELTEVLRGMGGLSAEERPVIGQVANLVRSAIEEIISTKQEAFQQQET 84 +ND+RV+YLGKKG +TEVLRGMG LSAEERP +G +AN VR I I+ K E +Q+E Sbjct: 25 VNDIRVQYLGKKGPITEVLRGMGKLSAEERPKMGALANEVRERIAAAITAKNEQLEQEEM 84 Query: 85 LNRLNAEKVDVTLPGRRLPQGGIHPVSRVVREIEDIFIGMGYRVAEGPEVETDYYNFEAL 144 +L+++ +DVTLPG ++ GG HP++ V+ EIED+FIGMGY V EGPEVETDYYNFEAL Sbjct: 85 NKKLSSQTIDVTLPGSQVNIGGRHPLTVVIEEIEDLFIGMGYTVEEGPEVETDYYNFEAL 144 Query: 145 NLPKNHPARDMQDSFYLTEDILMRTQTSPVQIRTMQAMKGETPVKIICPGKVFRRDDDDA 204 NLPK HPARDMQDSFY+TE++LMRTQTSPVQ RTM+ KG+ PVKIICPGKV+RRD+DDA Sbjct: 145 NLPKEHPARDMQDSFYITEEMLMRTQTSPVQTRTMEKHKGKGPVKIICPGKVYRRDNDDA 204 Query: 205 THSFQFHQIEGLVIGRHIRMSDLKGTLQQFVQEMFGPNTGIRLRPSFFPFTEPSVEVDVS 264 THS QF QIEGLV+ R I MSDLKGTL+ ++MFG + IRLRPSFFPFTEPSVEVDV+ Sbjct: 205 THSHQFMQIEGLVVDRKISMSDLKGTLELVAKKMFGQDREIRLRPSFFPFTEPSVEVDVT 264 Query: 265 CFKCGGHGCRLCKQSGWLEILGAGMVHPNVLEKGGYDPAEYSGFAFGMGVERIAMLKYGI 324 CFKCGG GC +CK++GW+EILGAGMVHPNVL+ G++P EY GFAFGMGVERIAMLKYGI Sbjct: 265 CFKCGGQGCSVCKKTGWIEILGAGMVHPNVLKMAGFNPEEYQGFAFGMGVERIAMLKYGI 324 Query: 325 DDIRYFYTNDMAFVKQFK 342 +DIR+FYTND+ F+ QFK Sbjct: 325 EDIRHFYTNDVRFISQFK 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,045,747,680 Number of extensions: 88146140 Number of successful extensions: 201312 Number of sequences better than 1.0e-05: 2506 Number of HSP's gapped: 195587 Number of HSP's successfully gapped: 2711 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)