BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0088.p (536 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4L8H4.1 C4L8H4_TOLAT Signal transduction histidine kinase regul... 459 e-127 A1JSJ2.1 A1JSJ2_YERE8 Two-component system, sensor kinase EC=2.7... 435 e-120 C4RZR6.1 C4RZR6_YERBE Putative uncharacterized protein 433 e-119 C4UVT3.1 C4UVT3_YERRO Putative uncharacterized protein 430 e-118 C4S935.1 C4S935_YERMO Putative uncharacterized protein 430 e-118 >C4L8H4.1 C4L8H4_TOLAT Signal transduction histidine kinase regulating citrate/malate metabolism Length = 536 Score = 459 bits (1182), Expect = e-127, Method: Compositional matrix adjust. Identities = 234/531 (44%), Positives = 336/531 (63%), Gaps = 2/531 (0%) Query: 1 LVKTKDYGLRTKVTIXXXXXXXXXXXXXYIIFRNQIIPQTRHALEDKATAIARTIALIPL 60 L +T LRT +T+ + ++ Q QTR +LEDKA A+ T+ P Sbjct: 2 LKQTPPLKLRTTITLLVCSVIALVLFVVHSVYLTQSTEQTRFSLEDKAKAVLHTLTAAPF 61 Query: 61 VSEGLNEGRSKE-IQAYTSKITRRNDIMFVVVIDMHSIRYSHPDPSRIGKPFVGGGQKAA 119 ++ L+ G K +Q Y ++ ++N+++F+VV+DM IR++HPDPS IGK F+GG + A Sbjct: 62 IASALSNGGDKAALQDYIEQVRQQNELLFIVVMDMQGIRHTHPDPSLIGKHFIGGDEVKA 121 Query: 120 XXXXXXXXXXXXXXXXXXRAFVPVYNNRGSQXXXXXXXLSMERVHLLIRQNEWTXXXXXX 179 R P+++ R Q +S E++ +I N W Sbjct: 122 LHGQESISEAKGTLGPSLRIISPLFDARHQQIGAIAVGISTEKIQEIIAANRWFAYWAIA 181 Query: 180 XXXXXXXXXXXXXXRKIKQMMFGMEPADISKLLQERSAMLQSTREGIIAVDHAARISMIN 239 RKIK++MFGMEP++I+ LL+ER+AMLQS REGIIAV+ +I++IN Sbjct: 182 FGGLVGSFGAFFLARKIKKIMFGMEPSEIASLLEERNAMLQSIREGIIAVNADEKITLIN 241 Query: 240 REAERLLGTAGITGNGMTRHIADYWPEL-RLEQVLSSGESRQDQELELNGITLLVSSVPI 298 EA+RLL G N + + +WP L L QVL++G++RQD+E+E NG TLL +SVP+ Sbjct: 242 DEAKRLLRQNGELENLLLTESSKHWPALLHLRQVLATGQARQDEEIEFNGSTLLTNSVPV 301 Query: 299 QVNGRIAGAIATFRDKTELAVLAERLSGISVYADALRAGAHEYMNKLHVIMGMTHMGLFD 358 +VNG++ GA+ TFRDKTE++ L +RLSGIS YA+ALR AHE+MNKLHVI+GM ++ +D Sbjct: 302 RVNGKVTGAVVTFRDKTEISQLLQRLSGISHYAEALRVQAHEFMNKLHVILGMVNIRAYD 361 Query: 359 ELQEYISGTVSNYQNEIGSITRQIKDPVMAGFLLGKLSRAREAGIELQLAEDSYLPESAD 418 +L+ YI T + Y E+GS+ RQIKDPV+AGF+LGK++R RE GI++ + SYLPESA+ Sbjct: 362 QLENYIMDTANYYHAEVGSLIRQIKDPVIAGFILGKMNRGREIGIDVNVTPTSYLPESAE 421 Query: 419 PQVIHQLITIVGNLLDNAMEVLEGQHMKEIELSFQYERGRLICTVRDNGPGIPEPLREQI 478 +V H+L+T++GNLL+NAM+ LEG I L+F ++ G L CTV DNG GI + I Sbjct: 422 SEVTHELVTVLGNLLENAMDALEGSDSAAIVLTFDHDDGHLRCTVSDNGQGIEPAVAAHI 481 Query: 479 FVQGFSTKGEQRGMGLYLVRKSVEKLQGQLKMIPGREPGTIFMADVPYAVK 529 F GFSTKG +RG+GL+LVR+S+EKL G ++ GT F+ +PYA K Sbjct: 482 FEHGFSTKGTRRGIGLFLVRQSLEKLGGTIECQSKPGEGTRFIVSLPYASK 532 >A1JSJ2.1 A1JSJ2_YERE8 Two-component system, sensor kinase EC=2.7.3.- Length = 558 Score = 435 bits (1118), Expect = e-120, Method: Compositional matrix adjust. Identities = 214/525 (40%), Positives = 326/525 (62%), Gaps = 2/525 (0%) Query: 9 LRTKVTIXXXXXXXXXXXXXYIIFRNQIIPQTRHALEDKATAIARTIALIPLVSEGLNEG 68 L T +T+ + +F Q+ + L+DKA A+AR+++ P + GL + Sbjct: 16 LSTSITLMVSAVIISVLLVVHTLFFVQLSKMAQEGLQDKAVAVARSLSFSPTIIHGLTDP 75 Query: 69 RS-KEIQAYTSKITRRNDIMFVVVIDMHSIRYSHPDPSRIGKPFVGGGQKAAXXXXXXXX 127 + K+IQ+YTS++ + N+++F+VV DM+ IRYSHP+P IG+ F+G + A Sbjct: 76 ETGKQIQSYTSQVQKANNLLFIVVTDMNGIRYSHPNPEMIGQHFIGNDLEPALQGKENSA 135 Query: 128 XXXXXXXXXXRAFVPVYNNRGSQXXXXXXXLSMERVHLLIRQNEWTXXXXXXXXXXXXXX 187 R F+PVY G Q +S+ + ++ + WT Sbjct: 136 INRGVLERALRIFIPVYEPHGKQIGVVAIGISLTSIDSVVSETRWTIPWTILFGALIGSL 195 Query: 188 XXXXXXRKIKQMMFGMEPADISKLLQERSAMLQSTREGIIAVDHAARISMINREAERLLG 247 + +K++M G EP +IS L ++R+AMLQS +EG+IAVD +RI+++N EA+RL Sbjct: 196 GTWFLVKALKRIMLGFEPYEISNLFEQRNAMLQSIKEGVIAVDANSRITVVNHEAKRLFK 255 Query: 248 TAGITGNGMTRHIADYWP-ELRLEQVLSSGESRQDQELELNGITLLVSSVPIQVNGRIAG 306 +G N + + YWP L LEQVL +G +R+D+E+ NG LL ++VP+ V G I G Sbjct: 256 QSGPMENLLLSSASKYWPVRLYLEQVLETGIARRDEEINFNGSILLTNTVPVMVKGDIIG 315 Query: 307 AIATFRDKTELAVLAERLSGISVYADALRAGAHEYMNKLHVIMGMTHMGLFDELQEYISG 366 AIATFRDKTE++ L +RL+G+ YADALRA +HE+MNKLHVI+G+ HM + +L++YI Sbjct: 316 AIATFRDKTEVSQLMQRLTGMVHYADALRAQSHEFMNKLHVILGLLHMKYYQQLEDYIVK 375 Query: 367 TVSNYQNEIGSITRQIKDPVMAGFLLGKLSRAREAGIELQLAEDSYLPESADPQVIHQLI 426 T +NYQ EIGS+ R++K P++AGFLLGK++RAR+ GI L +++DS LP++ + Q+ LI Sbjct: 376 TSNNYQAEIGSLLRKVKSPIIAGFLLGKINRARDLGITLSISDDSLLPDTDNEQITTTLI 435 Query: 427 TIVGNLLDNAMEVLEGQHMKEIELSFQYERGRLICTVRDNGPGIPEPLREQIFVQGFSTK 486 T++GNL++NAME + Q +EI +SF Y+ GR+ C V D+GPGI L++ IF GFSTK Sbjct: 436 TVLGNLIENAMEAIGNQSQREINVSFHYQNGRVHCVVSDDGPGIATELQDSIFDNGFSTK 495 Query: 487 GEQRGMGLYLVRKSVEKLQGQLKMIPGREPGTIFMADVPYAVKGD 531 G + G+GL LVR+S+E + G ++ T F ++PY V + Sbjct: 496 GNEHGIGLSLVRQSLESIGGNIEFDSEAGVFTQFFINLPYEVTSN 540 >C4RZR6.1 C4RZR6_YERBE Putative uncharacterized protein Length = 564 Score = 433 bits (1114), Expect = e-119, Method: Compositional matrix adjust. Identities = 216/520 (41%), Positives = 323/520 (62%), Gaps = 2/520 (0%) Query: 9 LRTKVTIXXXXXXXXXXXXXYIIFRNQIIPQTRHALEDKATAIARTIALIPLVSEGL-NE 67 L T +T+ + +F Q+ + +L++KA A+AR I+ P + GL N Sbjct: 16 LSTSITLMVSAVIISVLFVVHTLFFIQLSQMAQESLQNKAVAVARAISYSPTIINGLTNP 75 Query: 68 GRSKEIQAYTSKITRRNDIMFVVVIDMHSIRYSHPDPSRIGKPFVGGGQKAAXXXXXXXX 127 ++IQAYTS++ R ND++F+V+ DM IRYSHP+P IG+ F+G A Sbjct: 76 VAGQQIQAYTSEVQRANDLLFIVITDMKGIRYSHPNPEMIGQHFIGNDLLPALQGKEHSA 135 Query: 128 XXXXXXXXXXRAFVPVYNNRGSQXXXXXXXLSMERVHLLIRQNEWTXXXXXXXXXXXXXX 187 R F+P+Y +G Q +S+ + ++ + WT Sbjct: 136 INRGVLAQALRIFIPIYKPQGEQIGVVAIGISLTSIDSVVSETRWTIPWTILFGALIGSL 195 Query: 188 XXXXXXRKIKQMMFGMEPADISKLLQERSAMLQSTREGIIAVDHAARISMINREAERLLG 247 + +K++M G EP +IS L ++R+AMLQS +EG+IAVD +RI+++N EA+RL Sbjct: 196 GTWFLVKALKRIMLGFEPYEISNLFEQRNAMLQSIKEGVIAVDADSRITVVNHEAKRLFK 255 Query: 248 TAGITGNGMTRHIADYWP-ELRLEQVLSSGESRQDQELELNGITLLVSSVPIQVNGRIAG 306 +G N + H + YWP L L+QVL +G +R+D+E+ NG LL ++VP+ V G I G Sbjct: 256 QSGPMENLLLSHASQYWPVRLYLQQVLETGIARRDEEINFNGSILLTNTVPVMVKGNIIG 315 Query: 307 AIATFRDKTELAVLAERLSGISVYADALRAGAHEYMNKLHVIMGMTHMGLFDELQEYISG 366 AIATFRDKTE++ L +RL+G+ YADALRA +HE+MNKLHVI+G+ HM + +L++YI Sbjct: 316 AIATFRDKTEVSQLMQRLTGMVHYADALRAQSHEFMNKLHVILGLLHMKYYQQLEDYIVK 375 Query: 367 TVSNYQNEIGSITRQIKDPVMAGFLLGKLSRAREAGIELQLAEDSYLPESADPQVIHQLI 426 T +NYQ EIGS+ R++K PV+AGFLLGK++RAR+ GI L +++DS LP++ + QV LI Sbjct: 376 TSNNYQAEIGSLLRKVKSPVIAGFLLGKINRARDLGITLSISDDSLLPDTDNEQVTTTLI 435 Query: 427 TIVGNLLDNAMEVLEGQHMKEIELSFQYERGRLICTVRDNGPGIPEPLREQIFVQGFSTK 486 T++GNL++NAME + Q +EI +SF Y+ GR+ C V D+GPGI L+++IF GFSTK Sbjct: 436 TVLGNLIENAMEAIGNQGQREINVSFHYQHGRVHCVVSDDGPGISAELQDRIFDNGFSTK 495 Query: 487 GEQRGMGLYLVRKSVEKLQGQLKMIPGREPGTIFMADVPY 526 G + G+GL LVR+S+E + G ++ T F +PY Sbjct: 496 GNEHGIGLSLVRQSLESIGGNIEFDSEAGIFTQFFISLPY 535 >C4UVT3.1 C4UVT3_YERRO Putative uncharacterized protein Length = 569 Score = 430 bits (1106), Expect = e-118, Method: Compositional matrix adjust. Identities = 214/525 (40%), Positives = 323/525 (61%), Gaps = 2/525 (0%) Query: 9 LRTKVTIXXXXXXXXXXXXXYIIFRNQIIPQTRHALEDKATAIARTIALIPLVSEGL-NE 67 L T +T+ + +F Q+ + L+DKA A+AR ++ P + EGL N Sbjct: 22 LSTAITLMVSAVIISVLLVVHTLFFVQLSRMAQEGLQDKAVAVARALSFSPTIIEGLSNP 81 Query: 68 GRSKEIQAYTSKITRRNDIMFVVVIDMHSIRYSHPDPSRIGKPFVGGGQKAAXXXXXXXX 127 ++IQAYT ++ + N+++F+VV DM IRYSHP+P IG+ F+G + A Sbjct: 82 AAGEKIQAYTHQVQQANNLLFIVVTDMKGIRYSHPNPEMIGQHFIGNDLQPALQGKENHA 141 Query: 128 XXXXXXXXXXRAFVPVYNNRGSQXXXXXXXLSMERVHLLIRQNEWTXXXXXXXXXXXXXX 187 R F+PVY +G Q +S+ + ++ + WT Sbjct: 142 INRGVLERALRIFIPVYQPQGKQIGVVAIGISLTSIDSVVSETRWTIPWTILFGALIGSL 201 Query: 188 XXXXXXRKIKQMMFGMEPADISKLLQERSAMLQSTREGIIAVDHAARISMINREAERLLG 247 + +K++M G EP +IS L ++R+AMLQS +EG+IAVD +RI+++N EA+RL Sbjct: 202 GTWFLVKALKRIMLGFEPYEISNLFEQRNAMLQSIKEGVIAVDANSRITVVNHEAKRLFK 261 Query: 248 TAGITGNGMTRHIADYWP-ELRLEQVLSSGESRQDQELELNGITLLVSSVPIQVNGRIAG 306 +G N + + + YWP L L+QVL +G +R+D+E+ NG LL ++VP+ V G I G Sbjct: 262 QSGPMENLLLSNASKYWPVRLYLQQVLETGIARRDEEINFNGSILLTNTVPVMVKGDIIG 321 Query: 307 AIATFRDKTELAVLAERLSGISVYADALRAGAHEYMNKLHVIMGMTHMGLFDELQEYISG 366 AIATFRDKTE++ L +RL+G+ YADALRA +HE+MNKLHVI+G+ HM + +L++YI Sbjct: 322 AIATFRDKTEVSQLMQRLTGMVHYADALRAQSHEFMNKLHVILGLLHMKYYQQLEDYIVK 381 Query: 367 TVSNYQNEIGSITRQIKDPVMAGFLLGKLSRAREAGIELQLAEDSYLPESADPQVIHQLI 426 T +NYQ EIGS+ R++K PV+AGFLLGK++RAR+ GI L +++DS LP++ + QV LI Sbjct: 382 TSNNYQAEIGSLLRKVKSPVIAGFLLGKINRARDLGITLSISDDSLLPDTDNEQVTTTLI 441 Query: 427 TIVGNLLDNAMEVLEGQHMKEIELSFQYERGRLICTVRDNGPGIPEPLREQIFVQGFSTK 486 T++GNL++N++E + Q +EI +SF YE GR+ C V D+GPGI L+ IF GFSTK Sbjct: 442 TVLGNLIENSLEAIGDQTQQEINVSFHYEHGRIHCVVSDDGPGIATELQSSIFDNGFSTK 501 Query: 487 GEQRGMGLYLVRKSVEKLQGQLKMIPGREPGTIFMADVPYAVKGD 531 G + G+GL LVR+S+E + G ++ T F +PY + + Sbjct: 502 GNEHGIGLSLVRQSLESIGGNIEFDSEEGVFTQFFVSLPYDITSN 546 >C4S935.1 C4S935_YERMO Putative uncharacterized protein Length = 570 Score = 430 bits (1105), Expect = e-118, Method: Compositional matrix adjust. Identities = 213/530 (40%), Positives = 327/530 (61%), Gaps = 2/530 (0%) Query: 9 LRTKVTIXXXXXXXXXXXXXYIIFRNQIIPQTRHALEDKATAIARTIALIPLVSEGLNEG 68 L T +T+ + +F Q+ + +L++KA A+AR ++ P + GL + Sbjct: 22 LSTSITLMVSAVIISVLFVVHTLFFIQLSQMAQESLQNKAVAVARALSFSPTIINGLTDP 81 Query: 69 RS-KEIQAYTSKITRRNDIMFVVVIDMHSIRYSHPDPSRIGKPFVGGGQKAAXXXXXXXX 127 + ++IQAYTS++ + ND++F+V+ +M IRYSHP+P IG+ F+G A Sbjct: 82 VAGQKIQAYTSEVQKANDLLFIVITNMEGIRYSHPNPEMIGQHFIGNDLLPALQGKENSA 141 Query: 128 XXXXXXXXXXRAFVPVYNNRGSQXXXXXXXLSMERVHLLIRQNEWTXXXXXXXXXXXXXX 187 R F+PVY +G Q +S+ + ++ + WT Sbjct: 142 INRGVLARALRIFIPVYAPQGKQIGVVAIGISLTSIDSVVNETRWTIPWTILFGALIGSL 201 Query: 188 XXXXXXRKIKQMMFGMEPADISKLLQERSAMLQSTREGIIAVDHAARISMINREAERLLG 247 + +K++M G EP +IS L ++R+AMLQS +EG+IAVD +RI+++N EA+RL Sbjct: 202 GTWFLVKALKRIMLGFEPYEISNLFEQRNAMLQSIKEGVIAVDADSRITVVNHEAKRLFK 261 Query: 248 TAGITGNGMTRHIADYWP-ELRLEQVLSSGESRQDQELELNGITLLVSSVPIQVNGRIAG 306 +G N + + + YWP L L+QVL +G +R+D+E+ NG LL ++VP+ V G + G Sbjct: 262 QSGPMENLLLSNASKYWPVRLYLQQVLETGIARRDEEINFNGSILLTNTVPVMVKGNVIG 321 Query: 307 AIATFRDKTELAVLAERLSGISVYADALRAGAHEYMNKLHVIMGMTHMGLFDELQEYISG 366 AIATFRDKTE++ L +RL+G+ YADALRA +HE+MNKLHVI+G+ HM + +L++YI Sbjct: 322 AIATFRDKTEVSQLMQRLTGMVHYADALRAQSHEFMNKLHVILGLLHMKYYQQLEDYIVK 381 Query: 367 TVSNYQNEIGSITRQIKDPVMAGFLLGKLSRAREAGIELQLAEDSYLPESADPQVIHQLI 426 T +NYQ EIGS+ R+IK PV+AGFLLGK++RAR+ GI L +++DS LP++ + QV LI Sbjct: 382 TSNNYQAEIGSLLRKIKSPVIAGFLLGKINRARDLGITLSISDDSLLPDTDNEQVTTTLI 441 Query: 427 TIVGNLLDNAMEVLEGQHMKEIELSFQYERGRLICTVRDNGPGIPEPLREQIFVQGFSTK 486 T++GNL++NAME + Q +EI +SF Y+ GR+ C V D+GPGI L+++IF GFSTK Sbjct: 442 TVLGNLIENAMEAIGSQSQREINVSFHYQNGRVHCVVSDDGPGISAELQDRIFDNGFSTK 501 Query: 487 GEQRGMGLYLVRKSVEKLQGQLKMIPGREPGTIFMADVPYAVKGDETNDH 536 G + G+GL LVR+S+E + G ++ T F +PY + + H Sbjct: 502 GNEHGIGLSLVRQSLESIGGDIEFDSEAGVFTQFFISLPYDITLNNMTSH 551 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,638,451,121 Number of extensions: 99783179 Number of successful extensions: 317501 Number of sequences better than 1.0e-05: 1706 Number of HSP's gapped: 318595 Number of HSP's successfully gapped: 1712 Length of query: 536 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 395 Effective length of database: 1,732,333,088 Effective search space: 684271569760 Effective search space used: 684271569760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)