BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0081.p (166 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB46.1 B3KB46_9BACI Regulatory protein AsnC/Lrp family 266 4e-70 B1D7C6.1 B1D7C6_9BACL Transcriptional regulator, AsnC family 245 9e-64 C4GDY5.1 C4GDY5_9FIRM Putative uncharacterized protein 181 2e-44 A5CYA7.1 A5CYA7_PELTS Transcriptional regulator 177 3e-43 Q9K7L0.1 Q9K7L0_BACHD Transcriptional regulator (Lrp/AsnC family) 173 4e-42 >B3KB46.1 B3KB46_9BACI Regulatory protein AsnC/Lrp family Length = 166 Score = 266 bits (681), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 131/166 (78%), Positives = 144/166 (86%) Query: 1 MKEMNXXXXXXXXXXXXDARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWD 60 M+E++ DAR S AL++TLLG EEE+VK +I E+ERDHVIVKYATVVNW Sbjct: 1 MEELSELKLKVLDLLKEDARRSPALLSTLLGVEEEEVKTSIAELERDHVIVKYATVVNWG 60 Query: 61 KVSDERVTALIEVQITPERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREV 120 KV DE+VTALIEVQITPERGRGFEGIAERIYL+PQVKSVYLMSGAYDLLVEVEG NLREV Sbjct: 61 KVEDEKVTALIEVQITPERGRGFEGIAERIYLFPQVKSVYLMSGAYDLLVEVEGGNLREV 120 Query: 121 ANFVSEKLSPIDAVLSTKTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 ANFVSEKLSPID+VLSTKTNF LKKYKQDGIIFEE +EDNRL+ISP Sbjct: 121 ANFVSEKLSPIDSVLSTKTNFILKKYKQDGIIFEERQEDNRLLISP 166 >B1D7C6.1 B1D7C6_9BACL Transcriptional regulator, AsnC family Length = 163 Score = 245 bits (626), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 119/149 (79%), Positives = 130/149 (87%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 DAR L+A +L E VKQAI ME +H+IVKYATVVNW KV DE+VTALIEVQITP Sbjct: 15 DARRDADLIAAMLNEPVESVKQAIAYMEEEHIIVKYATVVNWSKVDDEKVTALIEVQITP 74 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 ERGRGFEGIAERIYLYP+VKSVYLMSGAYDLLVE+EG+NL+EVA+FVS KLSPID VLST Sbjct: 75 ERGRGFEGIAERIYLYPEVKSVYLMSGAYDLLVEIEGKNLKEVASFVSNKLSPIDKVLST 134 Query: 138 KTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 KTNF LKKYKQDGIIFEEHE D+RLMISP Sbjct: 135 KTNFILKKYKQDGIIFEEHEGDHRLMISP 163 >C4GDY5.1 C4GDY5_9FIRM Putative uncharacterized protein Length = 163 Score = 181 bits (458), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 80/148 (54%), Positives = 114/148 (77%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 +AR S +A ++GA+E V+ AIE+++ DHVI Y T+++W++ +E V+A+IEV +TP Sbjct: 15 NARISIHDLAAMIGADETSVRNAIEQLQEDHVIAGYLTLIDWERAGNETVSAMIEVSVTP 74 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 +RG GF+ +AERIY YP+V SVYLMSG +DL+V +EG+ LREV++FVS+KLS +D+VLST Sbjct: 75 QRGMGFDSVAERIYRYPEVNSVYLMSGGFDLMVTIEGKTLREVSSFVSDKLSTLDSVLST 134 Query: 138 KTNFTLKKYKQDGIIFEEHEEDNRLMIS 165 KTNF LKKYK G I E D+R+ +S Sbjct: 135 KTNFILKKYKDHGAILSEESHDSRMKVS 162 >A5CYA7.1 A5CYA7_PELTS Transcriptional regulator Length = 159 Score = 177 bits (448), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 84/149 (56%), Positives = 117/149 (78%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 +AR + +AT+LG EE +V + I+EME VI+ Y T+VNW+K +E+V+ALIEV+I+P Sbjct: 11 NARLTPGQIATMLGMEEGEVTRIIKEMEEKKVILGYYTLVNWEKAGEEKVSALIEVKISP 70 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 +R GF+ +AERIY +P+VK+V LMSGAYDL V VEG+NL+EVANFV++KL+ +D VLST Sbjct: 71 QREVGFDAVAERIYRFPEVKTVRLMSGAYDLAVMVEGQNLKEVANFVAQKLASLDNVLST 130 Query: 138 KTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 T+F LK YK G+I E+ EED RL+++P Sbjct: 131 TTHFVLKTYKNQGVIVEDGEEDRRLVVTP 159 >Q9K7L0.1 Q9K7L0_BACHD Transcriptional regulator (Lrp/AsnC family) Length = 164 Score = 173 bits (439), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 79/150 (52%), Positives = 116/150 (77%), Gaps = 1/150 (0%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSD-ERVTALIEVQIT 76 + R +A ++ A EE+V I+++E DHVI+ Y+ V++W KV + E VTA+I+V++T Sbjct: 15 NGRVPIPTLAKMIDATEEEVTAIIKKLENDHVILSYSAVIDWSKVKEVETVTAMIDVKVT 74 Query: 77 PERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLS 136 P+RG GF+ +AERIY +P+VK++YLMSGAYDL V +EG+ + EVA FVSEKLS ID VLS Sbjct: 75 PQRGVGFDEVAERIYRFPEVKALYLMSGAYDLSVVIEGKTMSEVARFVSEKLSTIDTVLS 134 Query: 137 TKTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 T T+F LKKYK DG++F++ E+D R++++P Sbjct: 135 TTTHFQLKKYKHDGVVFKKDEDDKRIVVTP 164 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.314 0.132 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 812,739,093 Number of extensions: 30192357 Number of successful extensions: 76677 Number of sequences better than 1.0e-05: 274 Number of HSP's gapped: 77326 Number of HSP's successfully gapped: 274 Length of query: 166 Length of database: 3,060,696,203 Length adjustment: 125 Effective length of query: 41 Effective length of database: 1,883,069,328 Effective search space: 77205842448 Effective search space used: 77205842448 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)