BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0077.p (436 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DE42.1 B1DE42_9BACL Amidohydrolase 687 0.0 C0ZGX8.1 C0ZGX8_BREBN Putative uncharacterized protein 684 0.0 A5FDG1.1 A5FDG1_FLAJ1 Amidohydrolase 483 e-134 B5HP50.1 B5HP50_9ACTO Organophosphate acid anhydrase 375 e-102 Q9FCH3.1 Q9FCH3_STRCO Putative organophosphate acid anhydrase 370 e-100 >B1DE42.1 B1DE42_9BACL Amidohydrolase Length = 436 Score = 687 bits (1774), Expect = 0.0, Method: Compositional matrix adjust. Identities = 332/434 (76%), Positives = 384/434 (88%) Query: 1 MSSGEQSHPMAGVTAITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNAT 60 M +Q+ + +TAITN RIFDG ++I+ RHI+I+ II+VGG+IPA A +ID +NAT Sbjct: 1 MEKIKQNAALEQITAITNVRIFDGYQIIAPRHIVIKGESIISVGGDIPADATIIDGENAT 60 Query: 61 LMPGLIDAHVHTSIGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAV 120 L+PGLIDAHVHTSIGGLRDALKFGVTTELEMNG FT +GREIQL+N+ IADVRSAG A+ Sbjct: 61 LLPGLIDAHVHTSIGGLRDALKFGVTTELEMNGDFTGRGREIQLKNVDDIADVRSAGTAI 120 Query: 121 TAPGGHPDELLPDDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQVTTIEEAVKHV 180 TAPGGHPDELLPD EIP+FVLKELEKL+E+DR AML A+AHDHDE PQVTT+EEA+KHV Sbjct: 121 TAPGGHPDELLPDGDEIPEFVLKELEKLSEEDREAMLAAYAHDHDEIPQVTTVEEAIKHV 180 Query: 181 HTQVENGADYIKIMIEEGTVMGVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQS 240 HTQVENGADYIKIMIEEGTVMG PGLPVLS++ILK AV EAHK +K+V+AHVLTA SS++ Sbjct: 181 HTQVENGADYIKIMIEEGTVMGAPGLPVLSDEILKTAVTEAHKFDKLVIAHVLTALSSKA 240 Query: 241 AIRIGVDGLAHLFIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNS 300 AI GVDGL HLFIDRPE TSE+V +I DSGAFVTPCLVLN+SIIGNPASELA DPRV+S Sbjct: 241 AIDFGVDGLGHLFIDRPECTSELVKSIADSGAFVTPCLVLNSSIIGNPASELANDPRVHS 300 Query: 301 KLSQEWIDILNSSFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGA 360 KLS +WIDILNSSFNT+PQG+++NSFK+VM+LH AGVDILVGTDV+PVP+ NLGGLAHGA Sbjct: 301 KLSPDWIDILNSSFNTFPQGNMENSFKNVMDLHHAGVDILVGTDVAPVPVPNLGGLAHGA 360 Query: 361 SVHHEMQLLVQAGFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLVDGDPTTSISD 420 SVHHEMQLLV+AGFTP EALQSATSKPAR FGL DRGRI EG RADL+L++GDPTT+ISD Sbjct: 361 SVHHEMQLLVKAGFTPTEALQSATSKPARCFGLHDRGRITEGARADLILINGDPTTNISD 420 Query: 421 SLSIQAVWLKGEGQ 434 +LSI++VW G Q Sbjct: 421 TLSIKSVWFNGSQQ 434 >C0ZGX8.1 C0ZGX8_BREBN Putative uncharacterized protein Length = 436 Score = 684 bits (1764), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/419 (78%), Positives = 380/419 (90%) Query: 13 VTAITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDAHVHT 72 VTAITN RIFDG ++I+ RH++++ II+VGG+IPA A +ID +NATLMPGLIDAHVHT Sbjct: 13 VTAITNVRIFDGNQMIAPRHVVMKGESIISVGGDIPADATIIDGENATLMPGLIDAHVHT 72 Query: 73 SIGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAVTAPGGHPDELLP 132 SIGGLRDALKFGVTTELEMNG FT++GREIQL+N+ G+ADVRSAG A+TAPGGHPDELLP Sbjct: 73 SIGGLRDALKFGVTTELEMNGDFTKRGREIQLKNVNGVADVRSAGTAITAPGGHPDELLP 132 Query: 133 DDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQVTTIEEAVKHVHTQVENGADYIK 192 D EIP+FVLKELEKL+E+DR AML A+AH+H+E PQVTT+EEA+KHVHTQVENGADYIK Sbjct: 133 DGDEIPEFVLKELEKLSEEDREAMLAAYAHNHEEIPQVTTVEEAIKHVHTQVENGADYIK 192 Query: 193 IMIEEGTVMGVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAIRIGVDGLAHL 252 IMIEEGTVMG PGLPVLS++ILK AV EAHKLNK+V+AHVLTA SSQ AI GVDGL HL Sbjct: 193 IMIEEGTVMGAPGLPVLSDEILKTAVTEAHKLNKLVIAHVLTARSSQEAIDFGVDGLGHL 252 Query: 253 FIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSKLSQEWIDILNS 312 FIDRP TS +V +I SGAFVTPCLVLN+SIIGNPASELA DPRV+SKLS +WIDILNS Sbjct: 253 FIDRPAYTSALVNSIASSGAFVTPCLVLNSSIIGNPASELANDPRVHSKLSPDWIDILNS 312 Query: 313 SFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGASVHHEMQLLVQA 372 SFNT+PQG+++NSF++VM+LH+AGVDILVGTDV+PVP+ +LGGLAHGASVHHEMQLLV+A Sbjct: 313 SFNTFPQGNMENSFQNVMDLHRAGVDILVGTDVAPVPIPSLGGLAHGASVHHEMQLLVKA 372 Query: 373 GFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLVDGDPTTSISDSLSIQAVWLKG 431 GFTP+EALQSATSKPAR FGL DRGRIAEG RADL+LV+GDP T+ISD+LSI+AVW KG Sbjct: 373 GFTPIEALQSATSKPARCFGLVDRGRIAEGARADLILVNGDPITNISDTLSIKAVWFKG 431 >A5FDG1.1 A5FDG1_FLAJ1 Amidohydrolase Length = 432 Score = 483 bits (1244), Expect = e-134, Method: Compositional matrix adjust. Identities = 251/422 (59%), Positives = 320/422 (75%), Gaps = 5/422 (1%) Query: 15 AITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAA-VIDADNATLMPGLIDAHVHTS 73 AI NA +FDG+ VI + ++ G+I ++ ++PA VID + TL+PGLIDAHVHTS Sbjct: 8 AIINANVFDGKNVIGKKTVVFSGGVITSILNDLPADIDDVIDGEGCTLLPGLIDAHVHTS 67 Query: 74 IGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAVTAPGGHPDELLPD 133 + L+DALKFG+TTELEM GGFT KGRE QL + G ADVRSAGM +TAPGGHPDEL+P Sbjct: 68 VEFLKDALKFGITTELEMMGGFTSKGRESQLIGVTGAADVRSAGMGLTAPGGHPDELIPK 127 Query: 134 DGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDE---APQVTTIEEAVKHVHTQVENGADY 190 IP+FVLKE+EK+TE+++ A E + VT++ A+ V QV NGADY Sbjct: 128 GDGIPEFVLKEMEKMTEEEKAVFAAAHQAQKKEEELSADVTSLNGAIDFVRRQVNNGADY 187 Query: 191 IKIMIEEGTVMGVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAIRIGVDGLA 250 KIMIEEGTVM PGLP++ +L+AAV EAHKL KI +AHVLTA+++++A+ IGVDGLA Sbjct: 188 FKIMIEEGTVMNAPGLPLIETPVLQAAVDEAHKLGKIAIAHVLTAEAAKTAVEIGVDGLA 247 Query: 251 HLFIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSKLSQEWIDIL 310 HLFIDRP+ T +++ AI G FVTPCLVLN+SIIGN A +A D RV +KL+++W + Sbjct: 248 HLFIDRPDWTQDLIEAIASKGIFVTPCLVLNSSIIGNSACHVAHDERVENKLNEDWKMTM 307 Query: 311 NSSFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGASVHHEMQLLV 370 S FNT+P+GS++++F +V +LH AGVDIL GTDVS VP+ +LGGLAHG SVHHEMQLLV Sbjct: 308 CSCFNTFPEGSMEDNFNNVKDLHDAGVDILAGTDVS-VPMPHLGGLAHGVSVHHEMQLLV 366 Query: 371 QAGFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLVDGDPTTSISDSLSIQAVWLK 430 QAG +P+EALQSATS ++RF L DRG IAEG+RADL+LV GDPT +ISDSLSI +W + Sbjct: 367 QAGLSPIEALQSATSVTSKRFNLSDRGLIAEGMRADLLLVKGDPTKNISDSLSIMGIWKE 426 Query: 431 GE 432 GE Sbjct: 427 GE 428 >B5HP50.1 B5HP50_9ACTO Organophosphate acid anhydrase Length = 404 Score = 375 bits (964), Expect = e-102, Method: Compositional matrix adjust. Identities = 210/419 (50%), Positives = 277/419 (66%), Gaps = 22/419 (5%) Query: 13 VTAITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDAHVHT 72 +TAITNA+IFDGEK R +II G I VGGE P + ++D ATL+PGLIDAHVH+ Sbjct: 3 LTAITNAQIFDGEKSTGVRTVIIDGGRIARVGGEAPRDSEIVDGSGATLLPGLIDAHVHS 62 Query: 73 SIGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAVTAPGGHPDELLP 132 + G L AL+FGVTTELEM G T + R + ++ +ADVRS+G A+T PGGHP EL+P Sbjct: 63 APGSLALALRFGVTTELEMQGMNTRENRGLITED-DSLADVRSSGFAITPPGGHPSELMP 121 Query: 133 DDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQVTTIEEAVKHVHTQVENGADYIK 192 + G P L + L P +T EEA V + G+D+IK Sbjct: 122 E-GFRPTRDLPPVMPLM------------------PFSSTPEEAAAFVPQLLARGSDFIK 162 Query: 193 IMIEEGTVMGVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAIRIGVDGLAHL 252 MI++G+V G PGLP L + A V EA K + +AH LT +++++A G+DGL H+ Sbjct: 163 FMIDDGSVEGHPGLPSLDGATVAAGVAEAKKYGALTVAHALTVEATRTAAEAGIDGLTHV 222 Query: 253 FIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSKLSQEWIDILNS 312 F+D+P T+E++A IKD+G FV PC+ LNAS++G SELA DPRV ++L W L S Sbjct: 223 FMDQPH-TAEIIALIKDAGMFVIPCISLNASMMGITGSELADDPRVAARLDSAWERTLRS 281 Query: 313 SFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGASVHHEMQLLVQA 372 S++ YPQG LD+ + +V L AGVD+L GTD S +P GGLAHGAS+HHE+Q LV A Sbjct: 282 SYDRYPQGKLDDVYDTVRALDAAGVDVLAGTDAS-MPETFFGGLAHGASLHHELQYLVAA 340 Query: 373 GFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLVDGDPTTSISDSLSIQAVWLKG 431 G TP AL++AT+ AR F L DRGRIAEGLRADL+LVDGDPT++ISD+L+ +AVW +G Sbjct: 341 GLTPARALRAATATTARCFRLSDRGRIAEGLRADLLLVDGDPTSNISDTLNTRAVWRRG 399 >Q9FCH3.1 Q9FCH3_STRCO Putative organophosphate acid anhydrase Length = 404 Score = 370 bits (950), Expect = e-100, Method: Compositional matrix adjust. Identities = 209/419 (49%), Positives = 275/419 (65%), Gaps = 22/419 (5%) Query: 13 VTAITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDAHVHT 72 +TAITNA++FDGEK + R ++I I VGGE+P V+D ATL+PGLIDAHVH+ Sbjct: 3 LTAITNAQVFDGEKTVGVRTVVIDGTRIARVGGEVPRDCEVVDGSGATLLPGLIDAHVHS 62 Query: 73 SIGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAVTAPGGHPDELLP 132 + G L AL+FGVTTELEM G T + R + + +ADVRS+G A+T PGGHP EL+P Sbjct: 63 APGSLALALRFGVTTELEMQGMNTRENRG-SIADDDTLADVRSSGFAITPPGGHPSELMP 121 Query: 133 DDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQVTTIEEAVKHVHTQVENGADYIK 192 + G P + L + L P TT EEA +V + G+D+IK Sbjct: 122 E-GFRPKWDLPPVMPLM------------------PFSTTPEEAAAYVPQLLARGSDFIK 162 Query: 193 IMIEEGTVMGVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAIRIGVDGLAHL 252 MI++GTV G PGLP L L A V EA K + +AH LT ++++ A GVDG+ H+ Sbjct: 163 FMIDDGTVEGHPGLPSLDRATLNAGVAEARKYGALTVAHALTVEATRMAAEAGVDGVTHV 222 Query: 253 FIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSKLSQEWIDILNS 312 F+D+P T+E++ I+D+G FV PC+ LNAS++G SELA DPRV ++L W L S Sbjct: 223 FMDQPH-TAEIIDLIRDAGMFVIPCITLNASMMGVTGSELADDPRVAARLDDSWDRTLRS 281 Query: 313 SFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGASVHHEMQLLVQA 372 S++ YPQGSL++ +V L AGVD+L GTDVS +P GGLAHGAS+HHE+Q LV A Sbjct: 282 SYDRYPQGSLEDVCATVRALDAAGVDVLAGTDVS-MPETFFGGLAHGASLHHELQYLVAA 340 Query: 373 GFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLVDGDPTTSISDSLSIQAVWLKG 431 G TP AL++AT+ ARRF L DRGR+AEGLRADL+LVDGDPT I D+L+ +AVW +G Sbjct: 341 GLTPSRALRAATATTARRFRLSDRGRVAEGLRADLLLVDGDPTGDIGDTLNTRAVWRRG 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,617,168,303 Number of extensions: 112741528 Number of successful extensions: 343429 Number of sequences better than 1.0e-05: 438 Number of HSP's gapped: 343558 Number of HSP's successfully gapped: 454 Length of query: 436 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 297 Effective length of database: 1,751,175,118 Effective search space: 520099010046 Effective search space used: 520099010046 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)