BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0076.p (300 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH30.1 B3KH30_9BACI Alpha/beta hydrolase fold 224 1e-56 B1KVD8.1 B1KVD8_CLOBM Carboxylesterase 109 3e-22 A6LZG6.1 A6LZG6_CLOB8 Putative uncharacterized protein 100 2e-19 B5HAR3.1 B5HAR3_STRPR Carboxylesterase 100 2e-19 A8LA69.1 A8LA69_FRASN Alpha/beta hydrolase fold 98 8e-19 >B3KH30.1 B3KH30_9BACI Alpha/beta hydrolase fold Length = 293 Score = 224 bits (570), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 114/290 (39%), Positives = 173/290 (59%), Gaps = 2/290 (0%) Query: 6 VKVFKSEAGKEAVLHSYNMLLRQWNTEIQELDIETPYGTTHCIVAGNAANPPLLLFHGVG 65 +K FKS G++ V SY+ LL W ++ +I T YG TH I G++ PPLLL HG G Sbjct: 1 MKRFKSNHGQKLVYASYDRLLSSWTVPYEQHNISTSYGMTHVITVGSSDLPPLLLLHGTG 60 Query: 66 DNSAVMWLLNMKELVRHFYCIAVDTIGGPGKSVPNGNYSKQTFRQVEWISSIADSLNLTT 125 DN A+MW+ N++EL R+F+ IAVD IGG GKS PN Y+++ F Q W+ + +++N+ Sbjct: 61 DNVAMMWIYNVEELSRNFHIIAVDNIGGSGKSEPNDKYARE-FNQATWLDEVLEAMNVDA 119 Query: 126 FNIAGVSNGAYMAFNYATQKSERVERVVCMEGGMVTAPFKAMLQTLLMMFPEILVPTRNN 185 IAGVS GAY+A++YA + +RV R+VC+ G + + F+ M + + PE L P+ N Sbjct: 120 VYIAGVSYGAYLAYHYAIVRPDRVNRIVCLAGSIAGSQFEVMSKMMRAFLPEALFPSERN 179 Query: 186 LLKVVDKLSSPDSRLSEKHPEVAEHLVLLMKNHNQQAMFIHKLELYDKEKAVAVKEKLYF 245 + ++ KL + E +PE+ +H L+K N ++M H + ++ A++ K F Sbjct: 180 VRSLLRKLCGTHADAFENNPELMQHWFYLLKYFNNRSMMKHAITIHSPADVQAIRSKTLF 239 Query: 246 LIGEYK-LDRKRDFIKLLTDGGFRYQVIPGAGHGINHEQPEAVNRETVGF 294 +IGE L + L G RY+++ GAGH INHE PE V+ E +GF Sbjct: 240 VIGERDLLSHYPKAVARLETNGMRYRIVNGAGHAINHEMPELVHEEIIGF 289 >B1KVD8.1 B1KVD8_CLOBM Carboxylesterase Length = 282 Score = 109 bits (273), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 60/170 (35%), Positives = 96/170 (56%), Gaps = 7/170 (4%) Query: 8 VFKSEAGKEAVLHSYNMLLRQWNTEIQELDIETPYGTTHCIVAGNAANPPLLLFHGVGDN 67 VFK++ GK V+ Y++ L++W ++L + T +G T I +G NPPL+L HG G N Sbjct: 6 VFKTKEGKNEVIKHYDLFLQKWTLSYEKLYVNTRHGKTFIIASGEKNNPPLILLHGSGMN 65 Query: 68 SAVMWLLNMKELVRHFYCIAVDTIGGPGKSVPNGNYSKQTFRQVEWISSIADSLNLTTFN 127 S VMWL +M++ ++ A+D +G PGKS N S EW+ + + L++ N Sbjct: 66 S-VMWLRDMQQYSHNYRVYAIDILGEPGKSDEN-RLSLNGSYYAEWLKDVFNDLSIKKAN 123 Query: 128 IAGVSNGAYMAFNYATQKSERVERVV--CMEG---GMVTAPFKAMLQTLL 172 + G+S GA+++ +A E+V+++V C G + FKAM TLL Sbjct: 124 VVGISLGAWLSIKFAVTYPEKVDKLVLLCPSGIGPQKKSFIFKAMAHTLL 173 >A6LZG6.1 A6LZG6_CLOB8 Putative uncharacterized protein Length = 283 Score = 100 bits (249), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 53/151 (35%), Positives = 85/151 (56%), Gaps = 2/151 (1%) Query: 5 MVKVFKSEAGKEAVLHSYNMLLRQWNTEIQELDIETPYGTTHCIVAGNAANPPLLLFHGV 64 M KVFK+ GK V+ Y+ L++ ++ ++T YG T I +G +NPPL+L HG Sbjct: 3 MGKVFKTNEGKNDVIKFYDKFLQKLALSYEKFYVDTKYGKTFIIASGEKSNPPLILLHGS 62 Query: 65 GDNSAVMWLLNMKELVRHFYCIAVDTIGGPGKSVPNGNYSKQTFRQVEWISSIADSLNLT 124 G NS VMWL ++KE + + A+D +G PGKS N S EW+ + ++L++ Sbjct: 63 GMNS-VMWLRDIKEYSKTYRVYAIDMLGEPGKSDEN-RPSLSGSSYAEWLKEVFENLSVE 120 Query: 125 TFNIAGVSNGAYMAFNYATQKSERVERVVCM 155 NI G+S GA++A ++ E ++V + Sbjct: 121 RANIIGISLGAWLAIKFSVSYPEMASKLVLL 151 >B5HAR3.1 B5HAR3_STRPR Carboxylesterase Length = 290 Score = 100 bits (248), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 53/168 (31%), Positives = 84/168 (50%), Gaps = 3/168 (1%) Query: 16 EAVLHSYNMLLRQWNTEIQELDIETPYGTTHCIVAGNAANPPLLLFHGVGDNSAVMWLLN 75 + L +Y ++ +W T + + TP+G TH V G A PPL+L G G ++ W Sbjct: 10 DGFLAAYEQVMAKWPTPRETATVPTPFGATHVNVCGPADGPPLVLLPGGGGATSASWYAQ 69 Query: 76 MKELVRHFYCIAVDTIGGPGKSVPNGNYSKQTFRQV-EWISSIADSLNLTTFNIAGVSNG 134 EL R AVD IG PG+S P + +T + EW+ ++ D L + + G S G Sbjct: 70 AAELSRRHRVYAVDLIGAPGRSTPADDRHPRTVADLAEWLDALLDGLGIAKTALGGHSYG 129 Query: 135 AYMAFNYATQKSERVERVVCMEGGMVTAPFKAMLQTLLMMFPEILVPT 182 A++A ++A + +RV+R+V + FK LL P +L P+ Sbjct: 130 AWIALHHALRAPDRVDRLVLFDPTQCFTGFKPAY--LLHALPMLLQPS 175 >A8LA69.1 A8LA69_FRASN Alpha/beta hydrolase fold Length = 290 Score = 98.2 bits (243), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 53/156 (33%), Positives = 82/156 (52%), Gaps = 3/156 (1%) Query: 5 MVKVFKSEAGKEAVLHSYNMLLRQWNTEIQELDIETPYGTTHCIVAGNAANPPLLLFHGV 64 M ++++EAG V Y +L W + L + T G T + +G +PPLLL HG Sbjct: 1 MSAIYRTEAGGNEVRRRYAEILSGWPVPAEHLCLPTREGETFVVASGAPNSPPLLLLHGS 60 Query: 65 GDNSAVMWLLNMKELVRHFYCIAVDTIGGPGKSVPNGNYSKQTFRQVEWISSIADSLNLT 124 G N+A MW+ ++ HF AVD IG PG S P + W+ + +L +T Sbjct: 61 GTNAA-MWMSDVPAWAEHFRVHAVDLIGEPGLSEPR-RPPLSSEACALWLDDVLGALGIT 118 Query: 125 TFNIAGVSNGAYMAFNYATQKSERVERVVCM-EGGM 159 + G S G ++A +YAT++ +RVER+V + GG+ Sbjct: 119 RTAVVGASLGGWLALDYATRRPDRVERLVALCPGGL 154 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,830,924,621 Number of extensions: 75003117 Number of successful extensions: 181065 Number of sequences better than 1.0e-05: 130 Number of HSP's gapped: 181463 Number of HSP's successfully gapped: 130 Length of query: 300 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 165 Effective length of database: 1,788,859,178 Effective search space: 295161764370 Effective search space used: 295161764370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)