BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0075.p (314 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K428.1 B3K428_9BACI Aminoglycoside phosphotransferase 179 3e-43 A6CLX8.1 A6CLX8_9BACI Putative uncharacterized protein 151 8e-35 A9FFQ4.1 A9FFQ4_SORC5 Putative uncharacterized protein 149 3e-34 A7Z263.1 A7Z263_BACA2 YerI 142 3e-32 O34640.1 YERI_BACSU Uncharacterized protein yerI 140 2e-31 >B3K428.1 B3K428_9BACI Aminoglycoside phosphotransferase Length = 320 Score = 179 bits (454), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 106/320 (33%), Positives = 165/320 (51%), Gaps = 16/320 (5%) Query: 6 VKKQAFAALGCDPEQAKLLGGYDSNVFEIN-RSGEEIVVKILENAITDEEALLSEFEWLE 64 V Q G KLLGG+D NVFE N G ++K L+ + + +++ E W+ Sbjct: 5 VLDQGAERFGRGSYSLKLLGGFDQNVFEYNGEDGRSFIMKFLDASKYRKASIIRELTWMA 64 Query: 65 YLRSEGLSAVKPILLNTGQYIFPV---SEKYCCVAYEKVEGTTLNPNDIMSWNPNVFEQW 121 YL +GL+ I I V S++Y +A+ K G+ L D+ S + + +QW Sbjct: 65 YLAEQGLNMAVSIRSAQDLLIEEVTHDSKRYHVIAFTKAPGSPLT--DVTS-DFAIIKQW 121 Query: 122 GMAIGKMHVLAK----SYQPVHKR--PQWFHHSLLKGGVLNN-DPILAEKWQGFKDAFKQ 174 G +G+MH L K + VH+ PQW H + L +WQ + + Sbjct: 122 GRGMGRMHKLGKKDATASSLVHRMAFPQWNDHVIFTDAFPEAAGERLHTQWQQYLKELES 181 Query: 175 LPVTRENFGLIHGDLHHSNVLLHQGNLTLLDFGDSEYHWFAYDIAISVYHIAYTVPAGPQ 234 LP +E +G++H DLHH N +H G + DFGD YHWFAYDIAI++YH TVP + Sbjct: 182 LPQDQEGYGIVHNDLHHHNFHVHNGEIIFFDFGDVSYHWFAYDIAIAIYHAVQTVPEN-R 240 Query: 235 RQAFVQAFFGSFMDGYAQENC-NIGFIPQIDTFIDYRHLFSYTYHTAYADWSQLTQGQVE 293 + FV F SF+ GY QEN + +I +I FID+R+++SY Y + +W+++ + + Sbjct: 241 KAEFVARFLDSFLSGYLQENTLSEEWIQRIPLFIDFRNIYSYVYFAKFVNWNEMDERTRK 300 Query: 294 FLGQMRSAILQDSPCLGFSL 313 +L M++ I + S+ Sbjct: 301 YLLAMKADIEAGKSVVKLSI 320 >A6CLX8.1 A6CLX8_9BACI Putative uncharacterized protein Length = 340 Score = 151 bits (381), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 92/319 (28%), Positives = 165/319 (51%), Gaps = 16/319 (5%) Query: 4 DEVKKQAFAALGCDPEQAKLLGGYDSNVFEINRSGEEIVVKILENAITDEEALLSEFEWL 63 +++ ++A G D AK LG +++ V+EI++ ++++ ++ ++E + +E EW+ Sbjct: 11 EDILRRAAEFYGGDSSNAKKLGDFENYVYEIHKGNTPYILRLTHSSHRNKEQVEAELEWV 70 Query: 64 EYLRSEGLSAVKPILLNTGQYIFPV----SEKYCCVAYEKVEGTTLN-PNDIMSWNPNVF 118 YL S+G++ N G + + S Y C+ ++K G ++ +D+M NP ++ Sbjct: 71 NYLHSQGVNVSLVSHSNEGNLVEEIPAGGSAFYVCL-FDKAPGVPVSVKSDMM--NPLLY 127 Query: 119 EQWGMAIGKMHVLAKSYQPVH-KRPQWFHHSLLKGG---VLNNDPILAEKWQGFKDAFKQ 174 E+WG IGKMH + K+Y+ H R W+ LLK + D +A + + Sbjct: 128 EEWGRTIGKMHRVTKNYKQAHIAREHWYEDDLLKNMSSYLPKEDAGIALEGKELMGKLLS 187 Query: 175 LPVTRENFGLIHGDLHHSNVLLHQGNLTLLDFGDSEYHWFAYDIAISVYHIAYTVPAGP- 233 LP ++ +GLIH D+H N H+G + + DF DS YHWFA DIAI +Y+ + Sbjct: 188 LPTEKDEYGLIHSDIHSGNFFCHEGEIHVFDFDDSSYHWFASDIAIPLYYSTWAKVGSKS 247 Query: 234 --QRQAFVQAFFGSFMDGYAQENC-NIGFIPQIDTFIDYRHLFSYTYHTAYADWSQLTQG 290 +R F + F F+ GY +EN +I +I F+ R + Y+ D S L++ Sbjct: 248 LQERSVFGEEFLTHFLKGYFRENTLEEVWIKRIPLFLKLRDIILYSVFHKKFDMSHLSEW 307 Query: 291 QVEFLGQMRSAILQDSPCL 309 + + +++ +LQD P + Sbjct: 308 EEGAVKGIKARLLQDEPMV 326 >A9FFQ4.1 A9FFQ4_SORC5 Putative uncharacterized protein Length = 329 Score = 149 bits (377), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 98/315 (31%), Positives = 146/315 (46%), Gaps = 17/315 (5%) Query: 6 VKKQAFAALGCDPEQAKLLGGYDSNVFEINRSGEEIVVKILENAITDEEALLSEFEWLEY 65 V +A A G DP + +GG++S VFE R V+K+ + +A+ +E EWLEY Sbjct: 15 VLDRALALYGLDPATCRHIGGFESQVFEARRRDGTFVLKLGDPGRRSVDAVEAECEWLEY 74 Query: 66 LRSEGLSAVKPILLNTGQY---IFPVSEKYCCVAYEKVEGTTLNPNDIMSWNPNVFEQWG 122 L + G+ A I G+Y + + AY K G L+ D+ P+V WG Sbjct: 75 LTANGVRAAPAIRAADGRYAALLGGPGQAVSVRAYAKAPGAPLSAGDL---TPDVATAWG 131 Query: 123 MAIGKMHVLAKSYQP--VHKRPQWFHHSLLKGGVL-----NNDPILAEKWQGFKDAFKQL 175 +G++H K Y P +R W DPI + + + L Sbjct: 132 RLLGRLHAATKVYVPPSARRRHAWHEDGDFTSARELLPEDERDPI-GGRLERLIAELRAL 190 Query: 176 PVTRENFGLIHGDLHHSNVLLHQGNLTLLDFGDSEYHWFAYDIAISVYHIAYTVPAGPQR 235 P R+ FGL+H D H NVL G LTL+DF D Y WFA D+ I ++++A P GP R Sbjct: 191 PTDRDAFGLVHTDAHFGNVLKDGGALTLIDFDDCAYEWFASDLGIVLFYVAID-PLGPGR 249 Query: 236 QA-FVQAFFGSFMDGYAQENC-NIGFIPQIDTFIDYRHLFSYTYHTAYADWSQLTQGQVE 293 ++ F F+DGYA+E+ + ++ ++D F+ R L YT + Sbjct: 250 PPELLRRFRDRFLDGYAREHALSARWLSEMDRFMKLRELVLYTAIRRVSRREVPDAWCRA 309 Query: 294 FLGQMRSAILQDSPC 308 +L + RS IL D P Sbjct: 310 YLERHRSRILGDQPA 324 >A7Z263.1 A7Z263_BACA2 YerI Length = 331 Score = 142 bits (359), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 94/295 (31%), Positives = 138/295 (46%), Gaps = 14/295 (4%) Query: 31 VFEINRSGEEIVVKILENAITDEEALLSEFEWLEYLRSEGLSAVKPILLNTGQYIFPVSE 90 V+EI + E ++KI E +L E EWL +L GLSA KPI G+ + V + Sbjct: 37 VYEIVKDNEPYILKITHTIRRTPEYILGEMEWLHHLAKGGLSAAKPIPSLNGKDVEEVPD 96 Query: 91 ----KYCCVAYEKVEGTTLNPNDIMSWNPNVFEQWGMAIGKMHVLAKSY---QPVHKRPQ 143 + YEK G + +D WN +F Q G G MH L KSY P +KR + Sbjct: 97 GKGGAFLVRVYEKAPGRKVEESD---WNGTLFYQLGKYTGNMHRLTKSYCLSDPTYKRQE 153 Query: 144 WFHHSLLK--GGVLNNDPILAEKWQGFKDAFKQLPVTRENFGLIHGDLHHSNVLLHQGNL 201 W LK V + ++ E+ + ++LP EN+GL+H DLHH N G + Sbjct: 154 WDEEEQLKLRKYVPADQHLVFERSDALMEKLRRLPKNPENYGLVHADLHHGNFNWDNGKI 213 Query: 202 TLLDFGDSEYHWFAYDIAISVYHIA-YTVPAGPQRQAFVQAFFGSFMDGYAQEN-CNIGF 259 T DF D Y+WF DI+I +Y++ Y V + AF F FM GY +EN + + Sbjct: 214 TAFDFDDIGYNWFVNDISILLYNMLWYPVIPYEDKAAFTGEFMTHFMKGYREENDLDPAW 273 Query: 260 IPQIDTFIDYRHLFSYTYHTAYADWSQLTQGQVEFLGQMRSAILQDSPCLGFSLA 314 + I F+ RH+ Y D + L + E L R I ++P ++ + Sbjct: 274 LEHIPDFLRLRHMLIYGLLHQAFDLNSLGDEEREMLKAFRRDIENETPITEYNFS 328 >O34640.1 YERI_BACSU Uncharacterized protein yerI Length = 336 Score = 140 bits (352), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 91/322 (28%), Positives = 152/322 (47%), Gaps = 14/322 (4%) Query: 4 DEVKKQAFAALGCDPEQAKLLGGYDSNVFEINRSGEEIVVKILENAITDEEALLSEFEWL 63 ++V +A A G +Q + L ++ V+E + + ++KI + ++ E EWL Sbjct: 15 EQVLAEAAARYGFSKDQVRFLADAENYVYECMKDNQPYILKITHTIRRSSDYMMGEMEWL 74 Query: 64 EYLRSEGLSAVKPILLNTGQYIFPVSE----KYCCVAYEKVEGTTLNPNDIMSWNPNVFE 119 +L G+S KP+ G+ + V + + YEK G ++ +D WN +F Sbjct: 75 RHLAIGGISVAKPLPSLNGKDVEAVPDGNGGSFLLRVYEKAPGQKVDESD---WNETLFY 131 Query: 120 QWGMAIGKMHVLAKSYQ---PVHKRPQWFHHSLLK-GGVLNNDPI-LAEKWQGFKDAFKQ 174 + G G MH L KSY+ P KR +W LK + D I + ++ + ++ Sbjct: 132 ELGRYTGSMHSLTKSYKLSNPAFKRQEWDEEEQLKLRKYVPEDQIKVFQQADSLMNELRR 191 Query: 175 LPVTRENFGLIHGDLHHSNVLLHQGNLTLLDFGDSEYHWFAYDIAISVYHIA-YTVPAGP 233 LP +++N+GL+H DLHH N G +T DF D Y+WF DI+I +Y++ Y V Sbjct: 192 LPKSQDNYGLVHADLHHGNFNWDHGKITAFDFDDIGYNWFVNDISILLYNVLWYPVVPYD 251 Query: 234 QRQAFVQAFFGSFMDGYAQEN-CNIGFIPQIDTFIDYRHLFSYTYHTAYADWSQLTQGQV 292 + AF + F FM GY +EN + ++ I F+ RH+ Y D + + + + Sbjct: 252 DKAAFTEEFMTHFMKGYWEENELDPAWLMIIPDFLRLRHMLIYGLLHQMFDLNTIGEEEK 311 Query: 293 EFLGQMRSAILQDSPCLGFSLA 314 E L R I +P F + Sbjct: 312 EMLAGFRRDIENGTPITAFDFS 333 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,993,165,465 Number of extensions: 88269173 Number of successful extensions: 268066 Number of sequences better than 1.0e-05: 164 Number of HSP's gapped: 267774 Number of HSP's successfully gapped: 164 Length of query: 314 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 179 Effective length of database: 1,788,859,178 Effective search space: 320205792862 Effective search space used: 320205792862 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)