BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0054.p (847 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value O87689.1 O87689_BACME Precorrin-3 methylase 431 e-118 C0Z7H9.1 C0Z7H9_BREBN Precorrin-3 methylase EC=2.1.1.- 422 e-116 B1HRF6.1 B1HRF6_LYSSC Cobalt-precorrin-3B C(17)-methyltransferas... 418 e-114 B7GLT9.1 B7GLT9_ANOFW Precorrin-3B methylase CobJ fused to 4Fe-4... 410 e-112 C5DAN3.1 C5DAN3_9BACI Precorrin-3B C17-methyltransferase 409 e-112 >O87689.1 O87689_BACME Precorrin-3 methylase Length = 540 Score = 431 bits (1108), Expect = e-118, Method: Compositional matrix adjust. Identities = 195/273 (71%), Positives = 239/273 (87%) Query: 4 QGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVS 63 +GKLL+IGFGPG EHIT RA A++ES+ +IGY TYV+LI+ LL Q+I+ TGMTEEVS Sbjct: 2 KGKLLVIGFGPGSFEHITQRAREAIQESDMIIGYKTYVELIQGLLTNQQIISTGMTEEVS 61 Query: 64 RAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQS 123 RAQEAV++AEAG+TVAVISSGDAGVYGMAGLVYEVLIE+GW + TGVE+EVIPG+SAI S Sbjct: 62 RAQEAVKQAEAGKTVAVISSGDAGVYGMAGLVYEVLIEKGWKKETGVELEVIPGISAINS 121 Query: 124 CSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEAR 183 C+SLLGAP+MHD+CTISLSDHLTPWE I R+EAA ADFV+AFYNP+SG+RTRQI EA+ Sbjct: 122 CASLLGAPVMHDACTISLSDHLTPWELIEKRIEAAAQADFVVAFYNPKSGRRTRQIVEAQ 181 Query: 184 QILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLMV 243 +ILL+YR P TPVG+VKSAYRDR++ ++T L+DML+HEIGML+TV+VGNS+T YD LM+ Sbjct: 182 RILLKYRSPDTPVGLVKSAYRDREEVVMTNLKDMLNHEIGMLTTVVVGNSSTFFYDDLMI 241 Query: 244 TPRGYERKYSLGADTQALKPHERLRTSSEPWSL 276 TPRGY+RKY+L Q L+PH+RLR +EPW+L Sbjct: 242 TPRGYQRKYTLNQTEQPLRPHQRLRKEAEPWAL 274 Score = 109 bits (273), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 51/145 (35%), Positives = 78/145 (53%) Query: 279 LNPTDNVVKVKTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLE 338 L+P +V +K C FC K A L ++L G +P+EL++ + GCGMAC AV E Sbjct: 390 LSPVGDVFTIKACDFCDGEKSDAIPHTEELQKRLGGMDMPKELKLGINGCGMACYGAVQE 449 Query: 339 DIGLVYSKGSYELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERF 398 DIG+VY KG+++L+LG K GR H+G V G++ +Y +G +ERF Sbjct: 450 DIGIVYRKGAFDLFLGAKTVGRNAHSGQIVAEGIAPDDIVEIVENIIHEYKEKGHPNERF 509 Query: 399 YAFFERMGSGISAHYPQAESALRCE 423 + FF+R+ + Y ++ E Sbjct: 510 HKFFKRVKNVYGFDYQDITPKIKVE 534 >C0Z7H9.1 C0Z7H9_BREBN Precorrin-3 methylase EC=2.1.1.- Length = 585 Score = 422 bits (1084), Expect = e-116, Method: Compositional matrix adjust. Identities = 195/275 (70%), Positives = 236/275 (85%), Gaps = 1/275 (0%) Query: 5 GKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVSR 64 GKL +IGFGPG EHIT RA AL+ES+ +IGY+TYVDLI+ LL Q+IV TGMTEEV+R Sbjct: 3 GKLFVIGFGPGSFEHITKRAREALQESDVIIGYSTYVDLIRGLLTNQQIVSTGMTEEVAR 62 Query: 65 AQEAVRRAEA-GQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQS 123 A+EAVR+AE G+ VAVISSGD+GVYGMAGLVYEVL+E+GW TGV +E++PG+SAI S Sbjct: 63 AREAVRQAEEEGKKVAVISSGDSGVYGMAGLVYEVLVEKGWTEATGVPIEIVPGISAINS 122 Query: 124 CSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEAR 183 C ++LGAPIMHD+CTISLSDHLTPWE IA R++AAG ADFVIA YNPRSG+RTRQI EA+ Sbjct: 123 CGAILGAPIMHDACTISLSDHLTPWELIAKRIDAAGMADFVIALYNPRSGRRTRQIVEAQ 182 Query: 184 QILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLMV 243 +ILL+YR P TPVGIVKSAYR+R+ +VTTL ML+H+IGML+TVI+GN++T VYDG M+ Sbjct: 183 RILLQYRSPDTPVGIVKSAYRERETVVVTTLAQMLEHDIGMLTTVIIGNTSTFVYDGKMI 242 Query: 244 TPRGYERKYSLGADTQALKPHERLRTSSEPWSLAA 278 TPRGY+RKY+L AD Q LKPH+RLR +EPWSL A Sbjct: 243 TPRGYQRKYTLSADEQPLKPHQRLRVENEPWSLEA 277 Score = 113 bits (283), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 51/128 (39%), Positives = 75/128 (58%) Query: 279 LNPTDNVVKVKTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLE 338 L+P +V++VK C FC K+ + A LHQ+L G+ +P+EL+I GCGMAC AV E Sbjct: 436 LSPIGDVLQVKACDFCDGEKKDSIPYAEELHQKLGGKEMPKELKIGFNGCGMACYGAVQE 495 Query: 339 DIGLVYSKGSYELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERF 398 DIG+V+ KG ++L+LG K GR H+G V G+ ++ +ERF Sbjct: 496 DIGIVFRKGKFDLFLGAKTVGRNAHSGIPVAEGIEKEEIVPLVERIVNRFKKDAFPNERF 555 Query: 399 YAFFERMG 406 + FF+R+G Sbjct: 556 HKFFQRVG 563 >B1HRF6.1 B1HRF6_LYSSC Cobalt-precorrin-3B C(17)-methyltransferase EC=2.1.1.- Length = 555 Score = 418 bits (1074), Expect = e-114, Method: Compositional matrix adjust. Identities = 189/276 (68%), Positives = 234/276 (84%) Query: 1 MNKQGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTE 60 M ++GK+ ++GFGPG +HIT RA+ AL++S+ +IGY TYV+LI+ L+ + IV TGMTE Sbjct: 1 MAQKGKIFVVGFGPGDFKHITTRAVEALQQSDMIIGYKTYVELIQDLVSAKSIVSTGMTE 60 Query: 61 EVSRAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSA 120 EVSRAQEAVR+AEAG V+VISSGDAGVYGMAGLVYEVLIE GW TG+EVE++PG+SA Sbjct: 61 EVSRAQEAVRQAEAGSIVSVISSGDAGVYGMAGLVYEVLIELGWTEATGIEVEIVPGISA 120 Query: 121 IQSCSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIE 180 I SC+SLLGAPIMHD+CTISLSDHLTPW IA RVEAA ADFVIA YNP+SG+RTRQI Sbjct: 121 INSCASLLGAPIMHDACTISLSDHLTPWNLIAKRVEAAAMADFVIALYNPKSGRRTRQIV 180 Query: 181 EARQILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDG 240 EA++ILL YR P TPVG+VKSAYR+RQ+ +TTL DML+H+IGML+TVI+GNS+T YD Sbjct: 181 EAQRILLEYRSPDTPVGLVKSAYRERQEVTMTTLADMLEHDIGMLTTVIIGNSSTFFYDN 240 Query: 241 LMVTPRGYERKYSLGADTQALKPHERLRTSSEPWSL 276 M+TPRGY+RKY+LG + Q L+PH+RLR +EPW++ Sbjct: 241 KMITPRGYQRKYTLGEEKQPLRPHQRLREENEPWAM 276 Score = 98.6 bits (244), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 46/145 (31%), Positives = 72/145 (49%) Query: 279 LNPTDNVVKVKTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLE 338 L P +V+ +K C FC K + A + ++L G +P+ L I GCGMAC AV + Sbjct: 405 LAPIGDVLSLKACDFCYGEKADSIPYAEEIQEKLGGLSLPKTLNIGFNGCGMACYRAVFD 464 Query: 339 DIGLVYSKGSYELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERF 398 DIG+VY K +++++G K GRT HA V G+ +Y A +ER Sbjct: 465 DIGIVYRKSKFDVFIGAKPVGRTAHAAQPVVEGLEPEQLIPLITAIIEEYKANAHPNERL 524 Query: 399 YAFFERMGSGISAHYPQAESALRCE 423 + +F+R+ + Y S ++ E Sbjct: 525 FKYFKRVKKILHFTYQDMSSKIKIE 549 >B7GLT9.1 B7GLT9_ANOFW Precorrin-3B methylase CobJ fused to 4Fe-4S reductase domain Length = 515 Score = 410 bits (1053), Expect = e-112, Method: Compositional matrix adjust. Identities = 189/273 (69%), Positives = 235/273 (86%) Query: 5 GKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVSR 64 GKL ++GFGPG +EH+T RA A+EES+ ++GY TYVDL+ L+ +E++ TGMTEEVSR Sbjct: 12 GKLFVVGFGPGSVEHMTKRAREAIEESDVIVGYKTYVDLVVDLIAGKEVISTGMTEEVSR 71 Query: 65 AQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQSC 124 AQEA+++AE G+ VAVISSGDAG+YGMAGLVYEVLIE+GW + G+EVE++PG+SAI SC Sbjct: 72 AQEAIKQAERGKNVAVISSGDAGLYGMAGLVYEVLIEKGWRKEDGIEVEIVPGISAIHSC 131 Query: 125 SSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEARQ 184 ++LLGAPIMHD+CTISLSDHLTPW IA R+EAA AADFVIA YNP+SG+RT+QI EA++ Sbjct: 132 AALLGAPIMHDACTISLSDHLTPWHVIAKRIEAAAAADFVIALYNPKSGRRTQQIVEAQR 191 Query: 185 ILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLMVT 244 ILL YR P TPVG+VKSAYR+RQ ++T+L DML+H+IGML+TVI+GNS+T VYDGLM+T Sbjct: 192 ILLTYRSPHTPVGLVKSAYRERQHIVLTSLGDMLEHDIGMLTTVIIGNSSTFVYDGLMIT 251 Query: 245 PRGYERKYSLGADTQALKPHERLRTSSEPWSLA 277 PRGYERKY L + Q LKPHERLR +EPWSLA Sbjct: 252 PRGYERKYKLASAVQPLKPHERLRPEAEPWSLA 284 Score = 118 bits (296), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 68/176 (38%), Positives = 90/176 (51%), Gaps = 12/176 (6%) Query: 229 IVGNSATMVYDGLMVTPRGYERKYSLGADTQALKPHERLRTSSEPWSLAALNPTDNVVKV 288 IVG T+ Y TP Y L + +P E +R E A PT NV + Sbjct: 330 IVGEKGTITY-----TPDHY-----LKVTMETERPDEVVRELLEAGLTVA--PTGNVFVM 377 Query: 289 KTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLEDIGLVYSKGS 348 K C FC K+ A A +L++Q G +P+EL++ GCGMAC AV EDIG+VY KG+ Sbjct: 378 KACDFCDGEKKDAIPYAEQLYKQFGGMELPKELRLGFNGCGMACYGAVHEDIGIVYRKGA 437 Query: 349 YELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERFYAFFER 404 ++L+LGGK GR H G V G+ QY +G +ERF+ FFER Sbjct: 438 FDLFLGGKTVGRNAHPGQLVAEGIHPDQLIETIARIIRQYKEEGYANERFHKFFER 493 >C5DAN3.1 C5DAN3_9BACI Precorrin-3B C17-methyltransferase Length = 520 Score = 409 bits (1050), Expect = e-112, Method: Compositional matrix adjust. Identities = 193/273 (70%), Positives = 234/273 (85%) Query: 4 QGKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQQEIVGTGMTEEVS 63 +GKLLI+GFGPG EH+T RA A+EES+ +IGY TY+DL+ L+ ++++ TGMTEEVS Sbjct: 2 KGKLLIVGFGPGSKEHMTKRAREAIEESDIIIGYKTYIDLVADLIGNKQVISTGMTEEVS 61 Query: 64 RAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQS 123 RAQEAV+ AE G+TVAVISSGDAGVYGMAGLVYEVLIE+GW R + +EVEVIPG+SAI S Sbjct: 62 RAQEAVKWAERGKTVAVISSGDAGVYGMAGLVYEVLIEKGWTRESDIEVEVIPGISAINS 121 Query: 124 CSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEAR 183 C++LLGAPIMHD+CTISLSDHLTPW I R+EAA AADFVIA YNP+SG+RTRQI EA+ Sbjct: 122 CAALLGAPIMHDACTISLSDHLTPWALIEKRIEAAAAADFVIALYNPKSGRRTRQIVEAQ 181 Query: 184 QILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHEIGMLSTVIVGNSATMVYDGLMV 243 +ILLRYR P+TPVG+VKSAYR RQ ++T L MLD++IGML+TVI+GNS+T VYDGLM+ Sbjct: 182 RILLRYRSPNTPVGLVKSAYRARQHIVLTDLAHMLDYDIGMLTTVIIGNSSTFVYDGLMI 241 Query: 244 TPRGYERKYSLGADTQALKPHERLRTSSEPWSL 276 TPRGY+RKY+L A Q LKPHERLR +EPW+L Sbjct: 242 TPRGYQRKYTLSASEQPLKPHERLRKEAEPWAL 274 Score = 114 bits (284), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 71/202 (35%), Positives = 99/202 (49%), Gaps = 16/202 (7%) Query: 224 MLSTVIVGNSATMVYDGLMVTPRGYERKYSLGADTQALKPHERLRTSSEPWSLAALN--P 281 M+ IVG TM+Y TP Y L + A P + E A L+ P Sbjct: 327 MVLADIVGEDGTMMY-----TPDHY-----LKLEVPASDPDRIIARLKE----AGLDVAP 372 Query: 282 TDNVVKVKTCGFCGMRKESAAGAARRLHQQLNGRLVPRELQISVAGCGMACSSAVLEDIG 341 +V+ VK C FC K+ A A L+ QL G +P+EL++ V GCGMAC AV EDIG Sbjct: 373 VGDVLTVKACDFCDGEKKDAIPYAEELYDQLGGMALPKELKLGVNGCGMACYGAVREDIG 432 Query: 342 LVYSKGSYELYLGGKKTGRTPHAGSKVRAGMSXXXXXXXXXXXXXQYCAQGSQDERFYAF 401 +VY KG+++L+LGGK GR H G V G+ +Y + +ERF+ F Sbjct: 433 IVYRKGAFDLFLGGKTVGRNAHPGQLVAEGIPPGEIVSVVTRIIQEYKERAHPNERFHKF 492 Query: 402 FERMGSGISAHYPQAESALRCE 423 F+R+ Y +A+ + E Sbjct: 493 FKRVKQVGGFVYQEAKQEAKIE 514 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,545,472,025 Number of extensions: 180649253 Number of successful extensions: 556602 Number of sequences better than 1.0e-05: 1595 Number of HSP's gapped: 556084 Number of HSP's successfully gapped: 1871 Length of query: 847 Length of database: 3,060,696,203 Length adjustment: 145 Effective length of query: 702 Effective length of database: 1,694,649,028 Effective search space: 1189643617656 Effective search space used: 1189643617656 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 136 (57.0 bits)