BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0049.p (394 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B7GLT4.1 B7GLT4_ANOFW Precorrin-3B methylase CobJ fused to preco... 372 e-101 C0Z7I4.1 C0Z7I4_BREBN CbiET protein 372 e-101 A3IB96.1 A3IB96_9BACI Precorrin-6Y C5,15-methyltransferase [deca... 372 e-101 C5DAN9.1 C5DAN9_9BACI Precorrin-6y C5,15-methyltransferase (Deca... 341 8e-92 Q5KZ01.1 Q5KZ01_GEOKA Precorrin-6Y C5,15-methyltransferase [deca... 340 2e-91 >B7GLT4.1 B7GLT4_ANOFW Precorrin-3B methylase CobJ fused to precorrin-6B methylase 2 CobL Length = 401 Score = 372 bits (956), Expect = e-101, Method: Compositional matrix adjust. Identities = 187/356 (52%), Positives = 252/356 (70%), Gaps = 17/356 (4%) Query: 26 VQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLASGDPLFFGI 85 + SE L+GGERQLSFF Y+GEKIV++N L + L + ++ V+LASGDP+F+GI Sbjct: 25 IDESERLVGGERQLSFFPHYKGEKIVIRNNLSSLVATLMN--ERKKTVILASGDPMFYGI 82 Query: 86 AGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRIDGKHKIA 145 YL ++ +EV P SS+Q AFA++G+SW DA +VSLHGR ++GLAQRIDGK K+A Sbjct: 83 GSYLAKKV---PIEVYPSVSSLQWAFAKMGESWHDAYIVSLHGRTMKGLAQRIDGKEKVA 139 Query: 146 LLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNFAPLNVVIL 205 +LTD+TN P I +YL +GMTEYEAFV E LGG E CRF L+E++ +FAPLN+VIL Sbjct: 140 VLTDDTNDPRAIASYLLSYGMTEYEAFVGENLGGENERCRFLSLEEMKDASFAPLNIVIL 199 Query: 206 RWKPGSGTAAVRRG----FAYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAVVWDI 261 R V +G F D+ F QR+PEKGLITK+EVR L L L+L E++VVWDI Sbjct: 200 R--------RVNKGPNWAFGIDDDEFLQRKPEKGLITKKEVRVLSLQALHLHEKSVVWDI 251 Query: 262 GSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLERLPD 321 G+ +G+VA E A+IAR G V+AIEK+A ++ + N ++FRADF +I +AP+ LE+ PD Sbjct: 252 GTCTGSVAIEAAKIAREGAVFAIEKNAYDIEICQKNMKKFRADFTLIHGRAPEHLEQFPD 311 Query: 322 PDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEV 377 PDA+FIGG+ G L+ IIA C RL+ GRIVI A+T+ETLY ++AL+ G ++ Sbjct: 312 PDAIFIGGTSGSLRDIIAVCCTRLKKGGRIVINAVTVETLYEAIDALRSYGYTVDI 367 >C0Z7I4.1 C0Z7I4_BREBN CbiET protein Length = 409 Score = 372 bits (955), Expect = e-101, Method: Compositional matrix adjust. Identities = 191/369 (51%), Positives = 250/369 (67%), Gaps = 9/369 (2%) Query: 18 LTPDSLSKVQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLAS 77 L P + ++ SE+L+GGER LSFF Y GEK VLK GL A ++L + R+ V+LAS Sbjct: 18 LLPLYRTWIEESELLVGGERHLSFFPEYTGEKRVLKGGLTAMVEELRT--ETRKTVILAS 75 Query: 78 GDPLFFGIAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQR 137 GDPLF+GI G L ++ +VE+ PH SS+QLAFA++G+SWQDA L S+HGR I+GLAQR Sbjct: 76 GDPLFYGIGGLLSKKL---NVEIYPHLSSIQLAFAKMGESWQDATLASVHGRSIKGLAQR 132 Query: 138 IDGKHKIALLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNF 197 IDGK K+ALLTD N+PA I YL F M EY+AFV E LG EE ++ L+E+ G+F Sbjct: 133 IDGKDKVALLTDRENSPAAIARYLLSFQMNEYDAFVAENLGSAEERTGWYSLEEMADGSF 192 Query: 198 APLNVVILRWKPGSGTAAVRRGFAYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAV 257 + LNVVIL+ + S F DE F QR+P+KGLITK+EVR L +++L L +++ Sbjct: 193 SDLNVVILKKRRPSPVWP----FGIADEEFSQRKPDKGLITKKEVRILSIAQLQLHAKSI 248 Query: 258 VWDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLE 317 VWDIG+ +G+VA E ARIAR G+VY +EK+ ++L N N +FR D +I +AP GL+ Sbjct: 249 VWDIGTCTGSVAIEAARIAREGEVYGVEKNVDDLENCRQNMAKFRTDLTVINARAPHGLD 308 Query: 318 RLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEV 377 PDPDAVFIGGSGGEL+ ++ C RLRP GRIV+ A TIETLY +A G T V Sbjct: 309 EFPDPDAVFIGGSGGELRELLNICCTRLRPNGRIVVNAATIETLYEATQAFAQEGFETSV 368 Query: 378 TQLXXXKGK 386 T + K Sbjct: 369 TLAQLSRSK 377 >A3IB96.1 A3IB96_9BACI Precorrin-6Y C5,15-methyltransferase [decarboxylating] (Precorrin-6 methyltransferase) Length = 400 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 181/369 (49%), Positives = 251/369 (68%), Gaps = 9/369 (2%) Query: 18 LTPDSLSKVQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLAS 77 L P ++ EVL+GGER L FF + GEKI++K GL A ++L E + R ++LAS Sbjct: 14 LFPQYRQWIEDCEVLVGGERVLDFFPHFTGEKIMIKGGLSALVERLSE--ETRNTIILAS 71 Query: 78 GDPLFFGIAGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQR 137 GDPLF+G+ GYL ++ +EV P+ SSVQLAFA++G+SWQDA + S+HGRP++GLAQR Sbjct: 72 GDPLFYGVGGYLAKKL---DLEVYPYMSSVQLAFAKMGESWQDAYVTSIHGRPMKGLAQR 128 Query: 138 IDGKHKIALLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNF 197 IDGK KIALLTD N+P + YL+ FGMTEY AFV E L G +E C ++ LDE+E +F Sbjct: 129 IDGKKKIALLTDAENSPNALARYLKHFGMTEYRAFVAENLQGEDEKCDWYLLDEMETAHF 188 Query: 198 APLNVVILRWKPGSGTAAVRRGFAYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAV 257 +PLNVVIL+ R DE F QR+P+KGLITK+E+R L L + L +++V Sbjct: 189 SPLNVVILQ----QTVEPKRYTLGIDDEEFSQRKPDKGLITKKEIRVLSLQAMQLQQDSV 244 Query: 258 VWDIGSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLE 317 +WDIG+ +G++A E ++A G+VYA+EK+A +L N N+++FR D I KAP+GL+ Sbjct: 245 IWDIGTCTGSMAIEAGKLAPEGEVYAVEKNAPDLENCLHNQQKFRVDITAIHSKAPEGLD 304 Query: 318 RLPDPDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEV 377 PDPDA+FIGG+GGE+ ++ C +RL+P GRIV+ A TIE LY +EA K A ++ Sbjct: 305 HFPDPDAIFIGGTGGEMVELLQLCCNRLKPNGRIVLNAATIENLYKAVEAFKACDFAVDI 364 Query: 378 TQLXXXKGK 386 Q + K Sbjct: 365 LQAQLARSK 373 >C5DAN9.1 C5DAN9_9BACI Precorrin-6y C5,15-methyltransferase (Decarboxylating), CbiE subunit EC=2.1.1.132 Length = 401 Score = 341 bits (874), Expect = 8e-92, Method: Compositional matrix adjust. Identities = 172/365 (47%), Positives = 242/365 (66%), Gaps = 17/365 (4%) Query: 26 VQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLASGDPLFFGI 85 + SE+L+GGER L+FF YQGE + ++ GL ++L + +R V++ASGDP+F+GI Sbjct: 25 IYESELLVGGERHLAFFPDYQGETLTIRGGLSQLVERLRH--ETKRTVIVASGDPMFYGI 82 Query: 86 AGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRIDGKHKIA 145 YL + +EV P SS+Q AFA++G+ WQDA+ +S+HGR ++GLAQRIDG K+A Sbjct: 83 GSYLSSKL---PIEVYPAVSSIQWAFAKMGEKWQDAKFISVHGRSMKGLAQRIDGCDKVA 139 Query: 146 LLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNFAPLNVVIL 205 +LTDETN+P I YL +GMTEY AFV E LG +E CRF+EL+E+ F PLNVVIL Sbjct: 140 ILTDETNSPNAIAKYLLSYGMTEYRAFVGENLGSQDERCRFFELEEMADAEFFPLNVVIL 199 Query: 206 RWKPGSGTAAVRRGFAYP----DEAFRQRRPEKGLITKREVRALVLSELNLFEEAVVWDI 261 + + G +P D+ F QR+P+KGLITK+EVR L +S L L ++VVWDI Sbjct: 200 Q--------KTKEGPMWPLGIDDDEFLQRKPDKGLITKKEVRVLSISALRLHAKSVVWDI 251 Query: 262 GSGSGAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLERLPD 321 G+ +G+VA E A+IAR G V+AIEK+A ++ + N ++FR D ++ KAP+ L++ D Sbjct: 252 GTCTGSVAIEAAKIAREGSVFAIEKNAHDIEICKENLKKFRVDITLVHGKAPEHLDKFAD 311 Query: 322 PDAVFIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEVTQLX 381 PDA+FIGG+ GE+ ++ C RL+ GRIV A+TIETL +E LK G +VT Sbjct: 312 PDAIFIGGTSGEMGPLLDVCCRRLKRNGRIVANAVTIETLAQAVEGLKQRGFQVDVTLAQ 371 Query: 382 XXKGK 386 + K Sbjct: 372 ISRSK 376 >Q5KZ01.1 Q5KZ01_GEOKA Precorrin-6Y C5,15-methyltransferase [decarboxylating] (Precorrin-6 methyltransferase) EC=2.1.1.132 Length = 401 Score = 340 bits (871), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 179/361 (49%), Positives = 235/361 (65%), Gaps = 9/361 (2%) Query: 26 VQRSEVLLGGERQLSFFGGYQGEKIVLKNGLKAFADKLEEVWKERRAVVLASGDPLFFGI 85 + SE+L+GGERQL+ F Y+GE IV++ GL ++L + RR VVLASGDPLF+GI Sbjct: 25 IDESELLVGGERQLAMFPDYKGETIVIRRGLAELVERLRH--ETRRTVVLASGDPLFYGI 82 Query: 86 AGYLVRRFGPEHVEVIPHYSSVQLAFARLGDSWQDAELVSLHGRPIRGLAQRIDGKHKIA 145 YL G +EV P SSVQ AFA++G+SWQDA VS HGRP+ GL QRIDG+ K+A Sbjct: 83 GSYLA---GKLPIEVYPAVSSVQWAFAKMGESWQDAAFVSAHGRPLTGLVQRIDGRRKVA 139 Query: 146 LLTDETNTPAVIGAYLREFGMTEYEAFVCERLGGGEEVCRFWELDELEKGNFAPLNVVIL 205 +LTDETN+P VI YL E+GMTEYEAFV E LGG E CR + L+E+ + F PLN+VIL Sbjct: 140 ILTDETNSPDVIARYLLEYGMTEYEAFVGEELGGPNERCRLFSLEEMAEAEFLPLNIVIL 199 Query: 206 RWKPGSGTAAVRRGFAYPDEAFRQRRPEKGLITKREVRALVLSELNLFEEAVVWDIGSGS 265 R S F D+ F QR+P+KGLITK+E+R L LS L L ++VVWDIG+ + Sbjct: 200 RQTAPSPVWP----FGIEDDEFFQRKPDKGLITKKEIRVLSLSALRLRPDSVVWDIGTCT 255 Query: 266 GAVAAECARIARCGKVYAIEKSAENLPNMEANRREFRADFEIIRDKAPQGLERLPDPDAV 325 G+VA E A+IAR G V+AIEK+ +++ N R+FR D ++ +AP L+ DPDA+ Sbjct: 256 GSVAIEAAKIARDGAVFAIEKNEQDIELCRQNLRKFRVDITLVHGRAPDRLDEFADPDAI 315 Query: 326 FIGGSGGELQHIIAHCTHRLRPEGRIVIGAITIETLYTGLEALKLAGMATEVTQLXXXKG 385 FIGG+ G + ++ C RL+ GRIVI A TIETL L G ++T + Sbjct: 316 FIGGTSGAMAPLLDVCCRRLKRNGRIVINAATIETLAEASRELGERGFHVDITLAQVSRS 375 Query: 386 K 386 K Sbjct: 376 K 376 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,348,369,930 Number of extensions: 102227456 Number of successful extensions: 225343 Number of sequences better than 1.0e-05: 740 Number of HSP's gapped: 223802 Number of HSP's successfully gapped: 745 Length of query: 394 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 256 Effective length of database: 1,760,596,133 Effective search space: 450712610048 Effective search space used: 450712610048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)