BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0048.p (273 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z7I5.1 C0Z7I5_BREBN Cobalt-precorrin-2 C(20)-methyltransferase 250 1e-64 A3IB97.1 A3IB97_9BACI Precorrin-2 methylase 241 5e-62 B1HRF1.1 B1HRF1_LYSSC Probable cobalt-precorrin-2 C(20)-methyltr... 238 6e-61 C5DAP0.1 C5DAP0_9BACI Precorrin-2 C20-methyltransferase 223 2e-56 A9U7Y8.1 A9U7Y8_PHYPA Predicted protein (Fragment) 218 5e-55 >C0Z7I5.1 C0Z7I5_BREBN Cobalt-precorrin-2 C(20)-methyltransferase Length = 236 Score = 250 bits (638), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 122/232 (52%), Positives = 152/232 (65%) Query: 42 GTLYGVGVGPGDPELITLKACRLLRECPVIAYPATKKGGKSYAHEIVEMHIKADEKVMLG 101 GTLYG+GVGPGDPELIT+KA RLL++ PV+AYP + G KSYAH+I E++++ +K MLG Sbjct: 5 GTLYGLGVGPGDPELITVKAFRLLQQSPVVAYPKKRMGSKSYAHQIAELYVQTPDKEMLG 64 Query: 102 LVFPMTRDPGQLASGWNRTAELCWNELKQGRDVAFVTEGDPNLYSTFIHLARLMQELHPG 161 LVFPMTRD L WN T E+ W L +G+DVAFVTEGDP YSTFIH+ R+M E HP Sbjct: 65 LVFPMTRDKEILEREWNNTVEIVWERLSEGKDVAFVTEGDPMFYSTFIHMMRVMHEEHPE 124 Query: 162 VPIVSIPGISSVXXXXXXXXXXXXXXDQRVGIIPATEDPEALKEALLHHDTXXXXXXXXX 221 VPIV++PG+SS ++++GIIPATED EA+++AL +HDT Sbjct: 125 VPIVTVPGVSSFLGAASRFNLPLADGEEQIGIIPATEDKEAMRKALENHDTVVFLKVAKV 184 Query: 222 XXXXXXXXXXXXXXXKASVVTKVTSPNETVWRDARELRGQELEYLSLMVVSK 273 KA+V TKVTS E VW D REL EL YL+LMVV K Sbjct: 185 LPMIIGLLKEMGLAEKAAVATKVTSSEEMVWTDMRELERAELSYLTLMVVKK 236 >A3IB97.1 A3IB97_9BACI Precorrin-2 methylase Length = 235 Score = 241 bits (615), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 121/232 (52%), Positives = 152/232 (65%), Gaps = 1/232 (0%) Query: 42 GTLYGVGVGPGDPELITLKACRLLRECPVIAYPATKKGGKSYAHEIVEMHIKADEKVMLG 101 G LYG+GVGPGDPELIT+KA R+++E PVIAYP +G KSYAH IV+++I +EK MLG Sbjct: 5 GVLYGLGVGPGDPELITVKAFRVIQESPVIAYPKKLRGSKSYAHRIVDVYINPEEKDMLG 64 Query: 102 LVFPMTRDPGQLASGWNRTAELCWNELKQGRDVAFVTEGDPNLYSTFIHLARLMQELHPG 161 LVFPMT+D L W ++ EL + +L++G+DVAFVTEGDP LYSTFIH+ +LMQ++HP Sbjct: 65 LVFPMTKDEAVLEREWTKSVELVYEKLQEGKDVAFVTEGDPLLYSTFIHMMKLMQDMHPD 124 Query: 162 VPIVSIPGISSVXXXXXXXXXXXXXXDQRVGIIPATEDPEALKEALLHHDTXXXXXXXXX 221 V I ++PGISS D RV IIPA +D EA++EA+ HD Sbjct: 125 VEIRTVPGISSFNGSASRLGIALADGDDRVAIIPAYDDYEAMREAIESHDAVVFIKVAKV 184 Query: 222 XXXXXXXXXXXXXXXKASVVTKVTSPNETVWRDARELRGQELEYLSLMVVSK 273 KASVVTKVTS E +W D REL G +LEYL+LMVV K Sbjct: 185 IDLMLQVLRDLDLLDKASVVTKVTSDEEVIW-DVRELDGVDLEYLTLMVVRK 235 >B1HRF1.1 B1HRF1_LYSSC Probable cobalt-precorrin-2 C(20)-methyltransferase EC=2.1.1.151 Length = 235 Score = 238 bits (606), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 119/232 (51%), Positives = 151/232 (65%), Gaps = 1/232 (0%) Query: 42 GTLYGVGVGPGDPELITLKACRLLRECPVIAYPATKKGGKSYAHEIVEMHIKADEKVMLG 101 G LYG+GVGPGDPELIT+KA R+++E PVIAYP +G KSYAH IV+++I +EK MLG Sbjct: 5 GVLYGLGVGPGDPELITVKAFRVIQESPVIAYPKKLRGSKSYAHRIVDVYINPEEKDMLG 64 Query: 102 LVFPMTRDPGQLASGWNRTAELCWNELKQGRDVAFVTEGDPNLYSTFIHLARLMQELHPG 161 LVFPMT+D L W ++ EL + +L++G+DVAFVTEGDP LYSTFIH+ +LMQ++HP Sbjct: 65 LVFPMTKDEAVLEREWTKSVELVYEKLQEGKDVAFVTEGDPLLYSTFIHMMKLMQDMHPD 124 Query: 162 VPIVSIPGISSVXXXXXXXXXXXXXXDQRVGIIPATEDPEALKEALLHHDTXXXXXXXXX 221 V I ++PGISS D RV IIPA +D A++EA+ HD Sbjct: 125 VEIRTVPGISSFNGSASRLGIALADGDDRVAIIPAYDDYAAMREAIESHDAVVFIKVAKV 184 Query: 222 XXXXXXXXXXXXXXXKASVVTKVTSPNETVWRDARELRGQELEYLSLMVVSK 273 +ASVVTKVTS E +W D REL G +LEYL+LMVV K Sbjct: 185 IDLMLQVLRDLDLLDQASVVTKVTSDEEVIW-DVRELDGVDLEYLTLMVVRK 235 >C5DAP0.1 C5DAP0_9BACI Precorrin-2 C20-methyltransferase Length = 233 Score = 223 bits (567), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 113/233 (48%), Positives = 145/233 (62%), Gaps = 1/233 (0%) Query: 41 TGTLYGVGVGPGDPELITLKACRLLRECPVIAYPATKKGGKSYAHEIVEMHIKADEKVML 100 +GTLYG+GVGPGDPEL+T+KA R L+E VI YP ++G KSYA +I+E + EK M+ Sbjct: 2 SGTLYGIGVGPGDPELLTVKAFRRLKEAHVITYPKKQRGSKSYAQQIIEAYFSPAEKEMI 61 Query: 101 GLVFPMTRDPGQLASGWNRTAELCWNELKQGRDVAFVTEGDPNLYSTFIHLARLMQELHP 160 GLVFPMT++ L WN TAE W +L G+DVAFVTEGDP LYSTFIHL ++M+E +P Sbjct: 62 GLVFPMTKNMDVLTEKWNETAEAIWEQLSAGKDVAFVTEGDPLLYSTFIHLMKIMKERYP 121 Query: 161 GVPIVSIPGISSVXXXXXXXXXXXXXXDQRVGIIPATEDPEALKEALLHHDTXXXXXXXX 220 V I +PG+SSV D++V IIPA +D EA+K+AL HD Sbjct: 122 NVTIEIVPGVSSVNAAAARLQIPLADGDEQVAIIPARDDYEAMKKALEDHDCVVFLKVAK 181 Query: 221 XXXXXXXXXXXXXXXXKASVVTKVTSPNETVWRDARELRGQELEYLSLMVVSK 273 A+VVTKVTS E +W + +L G ELEYL+L+VV K Sbjct: 182 VMGMMIRLLREQNLLQNAAVVTKVTSKEEIIW-NIEQLEGVELEYLTLLVVRK 233 >A9U7Y8.1 A9U7Y8_PHYPA Predicted protein (Fragment) Length = 335 Score = 218 bits (554), Expect = 5e-55, Method: Compositional matrix adjust. Identities = 99/132 (75%), Positives = 114/132 (86%) Query: 42 GTLYGVGVGPGDPELITLKACRLLRECPVIAYPATKKGGKSYAHEIVEMHIKADEKVMLG 101 GTLYGVGVGPGDPELIT+KA R++RECPV+AYP +KGGKSYAHEIVE+++ +EK MLG Sbjct: 187 GTLYGVGVGPGDPELITVKAYRMIRECPVVAYPKKRKGGKSYAHEIVELYVNPEEKEMLG 246 Query: 102 LVFPMTRDPGQLASGWNRTAELCWNELKQGRDVAFVTEGDPNLYSTFIHLARLMQELHPG 161 L+FPMT+DP L W +T E CW LK+G+DVAFVTEGDPNLYSTFIHLARLM+ELHP Sbjct: 247 LIFPMTKDPVVLEREWGKTVEACWGALKEGKDVAFVTEGDPNLYSTFIHLARLMRELHPE 306 Query: 162 VPIVSIPGISSV 173 VPI SIPGISSV Sbjct: 307 VPISSIPGISSV 318 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,458,141,198 Number of extensions: 56746099 Number of successful extensions: 120023 Number of sequences better than 1.0e-05: 657 Number of HSP's gapped: 119458 Number of HSP's successfully gapped: 657 Length of query: 273 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 139 Effective length of database: 1,798,280,193 Effective search space: 249960946827 Effective search space used: 249960946827 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)