BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0046.p (371 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5DAP2.1 C5DAP2_9BACI Cobalamin (Vitamin B12) biosynthesis CbiG ... 377 e-103 A4INZ6.1 A4INZ6_GEOTN Cobalamin biosynthesis protein CbiG 369 e-100 Q5KZ04.1 Q5KZ04_GEOKA Cobalamin biosynthesis protein 360 9e-98 B4BMN1.1 B4BMN1_9BACI Cobalamin (Vitamin B12) biosynthesis CbiG ... 360 9e-98 O87697.1 O87697_BACME CbiG protein 358 4e-97 >C5DAP2.1 C5DAP2_9BACI Cobalamin (Vitamin B12) biosynthesis CbiG protein Length = 357 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 194/354 (54%), Positives = 250/354 (70%), Gaps = 2/354 (0%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHGQVFAEPVKELLPKLFRQ 64 YA VAIT +G+ +A ++GE L + V+ K++ +D G ++F V+ LLP LF+ Sbjct: 2 YAIVAITKHGVDIARRVGEELPNARVYYTNKFARGDEDEKG-IRLFEGNVRLLLPSLFQT 60 Query: 65 YEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVAQ 124 Y GLIL SLGA VR+IAPLL DK+TDPAV+VID++GEHVIS+LSGHLGGAN LTRQVA+ Sbjct: 61 YRGLILIISLGAVVRMIAPLLKDKKTDPAVVVIDDKGEHVISVLSGHLGGANELTRQVAE 120 Query: 125 LMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGERG 184 ++ +HPVITTASDVQ AVDL G FGW E + VSAA+VN + VA +QESGER Sbjct: 121 ILHAHPVITTASDVQKTIAVDLFGRSFGWEWESDEKLTPVSAAVVNEQHVAIVQESGERE 180 Query: 185 WLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSLVLG 244 W LP ++R+ S+ E A AA+VVT R L ++E A L + V+YRP+ +VLG Sbjct: 181 WWNYDTPLPNNIRIYDSIDEALAAKPDAALVVTHRLLTKEEEAIL-QNGVLYRPKVIVLG 239 Query: 245 LGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELALFS 304 +GCNRGTT EE++AV+ DTL ++ S+ SV+ V T +K DE GLL + KYGWE ++ Sbjct: 240 IGCNRGTTAEEIDAVIRDTLEELRFSMKSVKAVCTIELKKDEPGLLEVVRKYGWEFVYYT 299 Query: 305 PEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVA 358 PEQLN V +EQPSE V++ TGAYGV EPAA L SGA L L KKK+GN TI+VA Sbjct: 300 PEQLNQVNIEQPSETVYRYTGAYGVSEPAAKLYSGAEKLELVKKKAGNVTISVA 353 >A4INZ6.1 A4INZ6_GEOTN Cobalamin biosynthesis protein CbiG Length = 394 Score = 369 bits (948), Expect = e-100, Method: Compositional matrix adjust. Identities = 196/360 (54%), Positives = 256/360 (71%), Gaps = 6/360 (1%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHG-QVFAEPVKELLPKLFR 63 YA VAIT +G+ +A +LG LA +V+ K++ D HG ++F+ V+ LLP+LF Sbjct: 14 YAVVAITKHGVEIARRLGGALAGADVYYTDKFARG--DEAKHGIRLFSGNVRALLPELFA 71 Query: 64 QYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVA 123 +Y+GLI SLGA VR+IAPLL DK+TDPAV+VID++GEHVIS+LSGHLGGAN LTR+VA Sbjct: 72 RYDGLICIISLGAVVRMIAPLLKDKKTDPAVVVIDDKGEHVISVLSGHLGGANELTRRVA 131 Query: 124 QLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGER 183 ++ + PVITTASDVQ AVDL G EFGW E + VSAA+VN E VA +QESGE Sbjct: 132 AILGARPVITTASDVQQTIAVDLFGREFGWEWESDDNLTPVSAAVVNEEPVAIVQESGEP 191 Query: 184 GWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQL-PEQEIAALGERTVVYRPRSLV 242 W P G LP+++ + GS+AE A +AA+VVT R L PE+E A+ + V+YRP+ + Sbjct: 192 NWWPAGRPLPKNITVYGSIAEALAARPTAALVVTHRLLAPEEE--AILQNGVLYRPKVIA 249 Query: 243 LGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELAL 302 LG+GCNRGT+ +E+EAV+ +TL ++ SL SV+ V T +K DE GLL + KY WEL Sbjct: 250 LGIGCNRGTSADEIEAVIHETLNELRFSLKSVKAVCTIDLKKDEQGLLEVVNKYRWELVA 309 Query: 303 FSPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARVSF 362 ++PE+LNTV +E PSE VF+ TGAYGV EPAA L SGA + L KKKSGN TI+VA + Sbjct: 310 YTPEELNTVPIEAPSETVFRYTGAYGVSEPAAKLYSGADRMALVKKKSGNVTISVALIDH 369 >Q5KZ04.1 Q5KZ04_GEOKA Cobalamin biosynthesis protein Length = 399 Score = 360 bits (925), Expect = 9e-98, Method: Compositional matrix adjust. Identities = 194/360 (53%), Positives = 256/360 (71%), Gaps = 6/360 (1%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHG-QVFAEPVKELLPKLFR 63 YA VAIT +G+ +A +LG LA +V+ K++ D HG ++F+ V+ LLP+LF Sbjct: 14 YAVVAITKHGVEIARRLGGALAGADVYYTDKFARG--DEAKHGIRLFSGNVRALLPELFA 71 Query: 64 QYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVA 123 +Y+GLI SLGA VR+IAPLL DK+TDPAV+VID++ EHVIS+LSGHLGGAN LTR+VA Sbjct: 72 RYDGLICIISLGAVVRMIAPLLKDKKTDPAVVVIDDKAEHVISVLSGHLGGANELTRRVA 131 Query: 124 QLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGER 183 ++ + PVITTASDVQ AVDL G +FGW E + VSAA+VN E VA +QESGE Sbjct: 132 AILSARPVITTASDVQQTIAVDLFGRQFGWEWESADKLTPVSAAVVNEEPVAIVQESGET 191 Query: 184 GWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQL-PEQEIAALGERTVVYRPRSLV 242 GW P G LP+++ + GS+AE A +AA+VVT R L PE+E A+ + V+YRP+ + Sbjct: 192 GWWPEGRPLPKNIAVYGSIAEALAARPAAALVVTHRLLAPEEE--AILQNGVLYRPKVIA 249 Query: 243 LGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELAL 302 LG+GCNRGT+ +E+EAV+ +TL ++ S+ SV+ V T +K DE GLL + KY WEL Sbjct: 250 LGIGCNRGTSADEIEAVIHETLNELRFSMKSVKAVCTIDLKKDEQGLLEVVNKYRWELVA 309 Query: 303 FSPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARVSF 362 ++PE+LNTV +E PSE V++ TGAYGV EPAA L SGA L L KKKSGN TI+VA + Sbjct: 310 YTPEELNTVSIEAPSETVYRYTGAYGVSEPAAKLYSGADRLALVKKKSGNVTISVALIDH 369 >B4BMN1.1 B4BMN1_9BACI Cobalamin (Vitamin B12) biosynthesis CbiG protein Length = 399 Score = 360 bits (925), Expect = 9e-98, Method: Compositional matrix adjust. Identities = 194/360 (53%), Positives = 256/360 (71%), Gaps = 6/360 (1%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKYSGNVQDATGHG-QVFAEPVKELLPKLFR 63 YA VAIT +G+ +A +LG LA +V+ K++ D HG ++F+ V+ LLP+LF Sbjct: 14 YAVVAITKHGVEIARRLGGALAGADVYYTDKFARG--DEAKHGIRLFSGNVRALLPELFA 71 Query: 64 QYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVA 123 +Y+GLI SLGA VR+IAPLL DK+TDPAV+VID++ EHVIS+LSGHLGGAN LTR+VA Sbjct: 72 RYDGLICIISLGAVVRMIAPLLKDKKTDPAVVVIDDKAEHVISVLSGHLGGANELTRRVA 131 Query: 124 QLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGER 183 ++ + PVITTASDVQ AVDL G +FGW E + VSAA+VN E VA +QESGE Sbjct: 132 AILSARPVITTASDVQQTIAVDLFGRQFGWEWESADKLTPVSAAVVNEEPVAIVQESGET 191 Query: 184 GWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQL-PEQEIAALGERTVVYRPRSLV 242 GW P G LP+++ + GS+AE A +AA+VVT R L PE+E A+ + V+YRP+ + Sbjct: 192 GWWPEGRPLPKNIAVYGSIAEALAARPAAALVVTHRLLAPEEE--AILQNGVLYRPKVIA 249 Query: 243 LGLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELAL 302 LG+GCNRGT+ +E+EAV+ +TL ++ S+ SV+ V T +K DE GLL + KY WEL Sbjct: 250 LGIGCNRGTSADEIEAVIHETLNELRFSMKSVKAVCTIDLKKDEQGLLEVVNKYRWELVA 309 Query: 303 FSPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARVSF 362 ++PE+LNTV +E PSE V++ TGAYGV EPAA L SGA L L KKKSGN TI+VA + Sbjct: 310 YTPEELNTVSIEAPSETVYRYTGAYGVSEPAAKLYSGADRLALVKKKSGNVTISVALIDH 369 >O87697.1 O87697_BACME CbiG protein Length = 374 Score = 358 bits (920), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 180/359 (50%), Positives = 251/359 (69%), Gaps = 4/359 (1%) Query: 5 YAAVAITANGIRLALKLGERLADTEVFCCAKY-SGNVQDATGHGQVFAEPVKELLPKLFR 63 YA VAIT +G+ +A LG ++V+ +K+ G+ Q+ + Q+F+ V+ LLP LF Sbjct: 18 YAVVAITKHGVEIARNLGRIFQQSDVYYMSKFEKGDEQEQ--NIQMFSGSVRMLLPSLFE 75 Query: 64 QYEGLILFFSLGAAVRLIAPLLADKRTDPAVIVIDERGEHVISMLSGHLGGANRLTRQVA 123 Y+GLI+ SLGA VR+IAP+L DK+TDPAV+VID++GE+VIS+LSGH+GGAN LTR+VA Sbjct: 76 SYKGLIIIISLGAVVRMIAPILKDKKTDPAVVVIDDKGENVISVLSGHIGGANELTREVA 135 Query: 124 QLMDSHPVITTASDVQGIFAVDLLGSEFGWRAEGFATMKTVSAALVNGEAVAFLQESGER 183 + +HPVITTASDVQ VDL G FGW E + VSA++VN E +A +QESGE+ Sbjct: 136 AALRAHPVITTASDVQKTIPVDLFGKRFGWVWESAENVTPVSASVVNEEEIAVVQESGEK 195 Query: 184 GWLPPGAVLPEHVRLCGSMAELQEATFSAAVVVTDRQLPEQEIAALGERTVVYRPRSLVL 243 W +P +++ S+ EA+ AA+VVT R L ++E A L E V+YRP+ L + Sbjct: 196 SWWHYEHPVPANIKTYSSIQTALEASPHAALVVTHRDLKKEEEAIL-ENGVLYRPKVLAI 254 Query: 244 GLGCNRGTTQEELEAVVLDTLADMGLSLLSVRNVATAAIKGDEAGLLGLCAKYGWELALF 303 G+GCNRGT+ E+E V+ TLA++ S+ SV+ + T +K DE GLL + +KYGWE + Sbjct: 255 GMGCNRGTSAAEIETVIEKTLAELQFSMKSVKALCTIELKKDEEGLLEVASKYGWEFVYY 314 Query: 304 SPEQLNTVVLEQPSEIVFKATGAYGVCEPAALLSSGARSLVLKKKKSGNATIAVARVSF 362 SP++LN++ ++QPS+ VFK TGAYGV EPAA+L SGA +L L KKKSGN TI+VA + + Sbjct: 315 SPQELNSISIQQPSDTVFKYTGAYGVSEPAAMLYSGADTLELVKKKSGNVTISVALIPY 373 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,033,617,346 Number of extensions: 80311188 Number of successful extensions: 206335 Number of sequences better than 1.0e-05: 513 Number of HSP's gapped: 205718 Number of HSP's successfully gapped: 560 Length of query: 371 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 234 Effective length of database: 1,770,017,148 Effective search space: 414184012632 Effective search space used: 414184012632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)