BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0045.p (574 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z7I8.1 C0Z7I8_BREBN Cobyrinic acid A,C-diamide synthase 263 5e-68 B7GLT0.1 B7GLT0_ANOFW Cobyrinic acid a,c-diamide synthase 244 3e-62 C1YJM2.1 C1YJM2_NOCDA Cobyrinate a,c-diamide synthase; hydrogeno... 242 8e-62 A4INZ5.1 A4INZ5_GEOTN Cobalamin biosynthesis protein CbiA 238 2e-60 B4BMN2.1 B4BMN2_9BACI Cobyrinic acid a,c-diamide synthase 238 2e-60 >C0Z7I8.1 C0Z7I8_BREBN Cobyrinic acid A,C-diamide synthase Length = 458 Score = 263 bits (671), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 126/196 (64%), Positives = 152/196 (77%) Query: 45 TVQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGL 104 TVQ FKCGPDYIDP YH AVTGR +RNLDS+M + E F+ SEGAD+S+IEGVMG+ Sbjct: 35 TVQGFKCGPDYIDPTYHTAVTGRASRNLDSFMLEHEIVKEIFVCGSEGADISIIEGVMGM 94 Query: 105 YDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNR 164 YDGKEAT GSTAEI++LTGSPVLLVV+ +SM RSAAAIV GFQ L P RI VI N+ Sbjct: 95 YDGKEATSDKGSTAEISILTGSPVLLVVNCQSMARSAAAIVKGFQLLNPQARIVGVIANK 154 Query: 165 CGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADL 224 GS H++ VKAAIE CGIPV+G+L R+ L+IPERHLGL+P+VERGEL PLF++ ADL Sbjct: 155 VGSEGHHKIVKAAIEQECGIPVVGYLKRENELEIPERHLGLVPSVERGELTPLFEKLADL 214 Query: 225 LEAGTDLEVLLQIAAA 240 + DLE + ++A A Sbjct: 215 IAETVDLEQIWELAKA 230 Score = 165 bits (418), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 82/204 (40%), Positives = 115/204 (56%), Gaps = 2/204 (0%) Query: 358 VIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATI 417 IAVA+D AF+FYY +N FSPL+GEG+P +ADG+Y+GGGFPEEFAA + Sbjct: 250 TIAVAKDPAFHFYYPENLELLEAYGAKLAFFSPLAGEGVPEDADGLYIGGGFPEEFAAEL 309 Query: 418 AGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDRRTA 477 + N ECGGYM L ++ G + M G+IP MQ + A Sbjct: 310 SQNEAVKQSIRSAITKGLPTLAECGGYMFLTEAIVTTDGDSYPMVGLIPGKVTMQKKLAA 369 Query: 478 LGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQPEGYARGN 537 LGYREV + L+ E+ +GHEFHYS +Y+ + AY+++G G++PEGYA+ N Sbjct: 370 LGYREVRGKEGNFLIGTEEQAKGHEFHYS--TYASDQELPAAYETKGLRGTKPEGYAQEN 427 Query: 538 IMAAYAHIHLASHIPAAIRLVEAC 561 ++A Y H+H S+ R + C Sbjct: 428 LVAGYTHLHFGSNPDLVERWLSLC 451 >B7GLT0.1 B7GLT0_ANOFW Cobyrinic acid a,c-diamide synthase Length = 457 Score = 244 bits (622), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 118/203 (58%), Positives = 143/203 (70%) Query: 45 TVQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGL 104 VQ FKCGPDYIDP YH AVTGR +RNLDSWM + + + SEGA LS+IEGVMGL Sbjct: 37 VVQGFKCGPDYIDPTYHTAVTGRRSRNLDSWMGNEDIVRYVCKKGSEGAHLSIIEGVMGL 96 Query: 105 YDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNR 164 +DGK AT GSTAEI+++T SPVLLVVD M RSAAA+V GFQ DVRI VI NR Sbjct: 97 FDGKRATTDEGSTAEISIITESPVLLVVDCSGMARSAAAVVRGFQLFHKDVRIVGVIANR 156 Query: 165 CGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADL 224 GS H+R VK AIE CGIPV+G+L + L IPERHLGL+P+VERGEL+P FDR + Sbjct: 157 VGSEGHFRLVKEAIEQQCGIPVVGYLQKTNDLTIPERHLGLVPSVERGELQPFFDRLGER 216 Query: 225 LEAGTDLEVLLQIAAAASVLRYP 247 + DL+++ ++A A + P Sbjct: 217 ITQTFDLDMIWELAEATELQSKP 239 Score = 149 bits (376), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 80/214 (37%), Positives = 115/214 (53%), Gaps = 5/214 (2%) Query: 348 ALASVYDPAPVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGG 407 L YD IAVA+DAAF+FYY +N FSPL+GE +P + G+Y+GG Sbjct: 242 TLKQTYDVR--IAVAKDAAFHFYYEENLEMLQAYGAELVYFSPLAGECVPNDVHGLYIGG 299 Query: 408 GFPEEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPA 467 GFPEEFAA +A + ECGG+M L + G +EM G+IP Sbjct: 300 GFPEEFAAELAQHEQVKQSVAEAIQRGLPTLAECGGFMYLTEGIETTDGQYYEMVGVIPG 359 Query: 468 HSVMQDRRTALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSG 527 H MQ ALGYRE+T A L +Q ++ +GHEFHYS ++ + + +AY+++G G Sbjct: 360 HVRMQRALVALGYREMTGMAGNFLFEQ-DKAKGHEFHYS--TFIRRQEEKFAYEAKGLRG 416 Query: 528 SQPEGYARGNIMAAYAHIHLASHIPAAIRLVEAC 561 + +GY ++A Y H + S+ R ++AC Sbjct: 417 CKRDGYMDKRLVAGYVHFYFPSNPLLVERWLKAC 450 >C1YJM2.1 C1YJM2_NOCDA Cobyrinate a,c-diamide synthase; hydrogenobyrinic acid a,c-diamide synthase (Glutamine-hydrolysing) EC=6.3.5.- EC=6.3.5.9 Length = 519 Score = 242 bits (617), Expect = 8e-62, Method: Compositional matrix adjust. Identities = 181/530 (34%), Positives = 246/530 (46%), Gaps = 48/530 (9%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 V K GPDYIDP YHA TGR RNLD + + L + GAD++VIEGVMGL+ Sbjct: 24 VSGHKVGPDYIDPGYHALATGRPGRNLDPHLVGVERVAPLLLHGARGADVAVIEGVMGLH 83 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 DG+ T S+A +A LT +PV+LVVDV M RS A+V G +P V + VI+N+ Sbjct: 84 DGRLGTDGFASSAHVAALTRTPVVLVVDVSRMSRSVGALVAGMAAYDPAVEVVGVILNQA 143 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADLL 225 GS + A I A +PV+G + RD+ L P RHLGL+PA ER E + +R + + Sbjct: 144 GSVRNV----AEITRATHLPVLGVVHRDERLASPSRHLGLVPASERAESARVVERLGEHV 199 Query: 226 EAGTDLEVLLQIAAAASVLRY----PAAGKILPEEGPAEELPSEAVPAARKILQETGVQG 281 DL+ LL +A +A L PA P G E P++A +ET V G Sbjct: 200 ARQVDLDALLAVARSAPALDARSWDPALALGTPAPGSLPEDPAQA--------RETSVPG 251 Query: 282 AQEXXXXXXXXXXXXXXXXKILQETGVQGAQESPGGQSADAPFPPTA-VKEAGNLLTYP- 339 + + +G E P ++ P A + + P Sbjct: 252 TP------------LEGRTRAWENSGPGTPPEDPARTREESQAPHAAHTDHETHTVRAPG 299 Query: 340 -PLDRSAPQALASVYDPA----PVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGE 394 P +R ++P PV+AVA AF F YA+ F PL Sbjct: 300 GPGERHTAHTDHGPHEPPASRRPVVAVAGGRAFTFRYAETEELLTAAGCEVVAFDPLGDT 359 Query: 395 GIPPEADGIYLGGGFPEEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDR 454 +PP G+YLGGGFPE +A +A N EC G + L SL Sbjct: 360 ALPPGTRGVYLGGGFPEVYAGELAANRGLLTQLREAARDGVPTVAECAGLLYLAESLDGE 419 Query: 455 AGAVHEMAGIIPAHSVMQDRRTALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPG- 513 MAG +PA + M R T L Y E + D LL + GER+ GHEFH + + +PG Sbjct: 420 -----PMAGAVPARARMTGRLT-LRYPEAVSPGDTLLTRAGERVTGHEFHRTAL--TPGA 471 Query: 514 EPQLYAYDSRGRSGSQPEGYARGNIMAAYAHIHLASHIPAAIRLVEACRS 563 L A++ G PEG+A + A+Y H+H A H A R +A R+ Sbjct: 472 RGDLPAWEIEG----APEGFASPTLHASYLHVHWAGHPRLAARFADAVRA 517 >A4INZ5.1 A4INZ5_GEOTN Cobalamin biosynthesis protein CbiA Length = 454 Score = 238 bits (606), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 116/196 (59%), Positives = 137/196 (69%) Query: 45 TVQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGL 104 TVQ FKCGPDYIDP YH AVTGR ARNLDSWM + +GAD+++IEGVMGL Sbjct: 32 TVQGFKCGPDYIDPTYHTAVTGRPARNLDSWMMGSEAVKTVLANGCQGADIAIIEGVMGL 91 Query: 105 YDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNR 164 +DGK+ GSTAEIA+LT SPVLLVVD M RSAAAIV GFQ PDVR+A V NR Sbjct: 92 FDGKQPLSDEGSTAEIAVLTESPVLLVVDCSGMARSAAAIVHGFQTFRPDVRLAGVFANR 151 Query: 165 CGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADL 224 GS H+R VKAA+E CG+PV+G+L +D+ L +PERHLGL+P+VERGEL+ F Sbjct: 152 VGSEGHFRLVKAAVEQTCGVPVVGFLTQDEALALPERHLGLVPSVERGELDSFFAELGRK 211 Query: 225 LEAGTDLEVLLQIAAA 240 + D E LL IA A Sbjct: 212 VARTIDWETLLSIAEA 227 Score = 164 bits (415), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 86/203 (42%), Positives = 112/203 (55%), Gaps = 2/203 (0%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 +AVA+DAAF+FYY +N FSPL+GE +P +G+Y+GGGFPEEFAA +A Sbjct: 247 VAVAKDAAFHFYYPENLEMLSACGAELVYFSPLAGEPLPDGVNGLYIGGGFPEEFAAELA 306 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDRRTAL 478 + ECGG+M L L GA +EMAG+IP VM+ + AL Sbjct: 307 RQAAVKQSIRAAIEHGLPTLAECGGFMFLTEQLVATDGAAYEMAGVIPGKVVMKTKLAAL 366 Query: 479 GYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQPEGYARGNI 538 GYREV R LL +GE RGHEFHYS Y P +AY++ GR G++P+GY + Sbjct: 367 GYREVRGRNGNFLLPEGETARGHEFHYS--VYEPRGEAPFAYETSGRKGTKPDGYLAHRL 424 Query: 539 MAAYAHIHLASHIPAAIRLVEAC 561 +A Y H H AS R + C Sbjct: 425 VAGYVHFHFASAPAMVERWLAEC 447 >B4BMN2.1 B4BMN2_9BACI Cobyrinic acid a,c-diamide synthase Length = 454 Score = 238 bits (606), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 116/196 (59%), Positives = 137/196 (69%) Query: 45 TVQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGL 104 TVQ FKCGPDYIDP YH AVTGR ARNLDSWM + +GAD+++IEGVMGL Sbjct: 32 TVQGFKCGPDYIDPTYHTAVTGRPARNLDSWMMGSEAVKTVLANGCQGADIAIIEGVMGL 91 Query: 105 YDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNR 164 +DGK+ GSTAEIA+LT SPVLLVVD M RSAAAIV GFQ PDVR+A V NR Sbjct: 92 FDGKQPLSDEGSTAEIAVLTESPVLLVVDCSGMARSAAAIVHGFQTFRPDVRLAGVFANR 151 Query: 165 CGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRAADL 224 GS H+R VKAA+E CG+PV+G+L +D+ L +PERHLGL+P+VERGEL+ F Sbjct: 152 VGSEGHFRLVKAAVEQTCGVPVVGFLTQDEALALPERHLGLVPSVERGELDSFFAELGRK 211 Query: 225 LEAGTDLEVLLQIAAA 240 + D E LL IA A Sbjct: 212 VARTIDWETLLSIAEA 227 Score = 164 bits (415), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 86/203 (42%), Positives = 112/203 (55%), Gaps = 2/203 (0%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 +AVA+DAAF+FYY +N FSPL+GE +P +G+Y+GGGFPEEFAA +A Sbjct: 247 VAVAKDAAFHFYYPENLEMLSACGAELVYFSPLAGEPLPDGVNGLYIGGGFPEEFAAELA 306 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSVMQDRRTAL 478 + ECGG+M L L GA +EMAG+IP VM+ + AL Sbjct: 307 RQAAVKQSIRAAIEHGLPTLAECGGFMFLTEQLVATDGAAYEMAGVIPGKVVMKTKLAAL 366 Query: 479 GYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQPEGYARGNI 538 GYREV R LL +GE RGHEFHYS Y P +AY++ GR G++P+GY + Sbjct: 367 GYREVRGRNGNFLLPEGETARGHEFHYS--VYEPRGEAPFAYETSGRKGTKPDGYLAHRL 424 Query: 539 MAAYAHIHLASHIPAAIRLVEAC 561 +A Y H H AS R + C Sbjct: 425 VAGYVHFHFASAPAMVERWLAEC 447 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,069,401,740 Number of extensions: 120494107 Number of successful extensions: 307954 Number of sequences better than 1.0e-05: 700 Number of HSP's gapped: 304913 Number of HSP's successfully gapped: 1371 Length of query: 574 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 433 Effective length of database: 1,732,333,088 Effective search space: 750100227104 Effective search space used: 750100227104 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)