BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0044.p (221 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB54.1 B3KB54_9BACI ROK family protein 293 7e-78 Q5KUZ7.1 Q5KUZ7_GEOKA Fructokinases EC=2.7.1.4 261 4e-68 C3J4H9.1 C3J4H9_9BACI ROK family protein 261 4e-68 B7YAV9.1 B7YAV9_9BACI ROK family protein 261 4e-68 A4IT14.1 A4IT14_GEOTN Fructokinase 252 2e-65 >B3KB54.1 B3KB54_9BACI ROK family protein Length = 298 Score = 293 bits (750), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 135/191 (70%), Positives = 156/191 (81%) Query: 1 VKVLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSF 60 + +LGAIEAGGTKFVCG+G EDGT+++RVSFPTTTPEETM V ++F K++EAIG+GSF Sbjct: 1 MSLLGAIEAGGTKFVCGVGTEDGTVVERVSFPTTTPEETMANVFNFFADKDIEAIGVGSF 60 Query: 61 GPIDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQ 120 GPIDPV GSPTYG ITTTPKPHW YN+V + F VP+GFDTDVNGAALGEY WGAAQ Sbjct: 61 GPIDPVKGSPTYGCITTTPKPHWSNYNIVKALEERFDVPIGFDTDVNGAALGEYTWGAAQ 120 Query: 121 GLDSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGL 180 GLDSCLY +L+HGLSHPEMGHI VRRHPED++EG CPYH+DCLEGL Sbjct: 121 GLDSCLYITIGTGVGAGAVVSGELIHGLSHPEMGHIYVRRHPEDQYEGTCPYHADCLEGL 180 Query: 181 AAGPAIGKRWG 191 AAGP++GKRWG Sbjct: 181 AAGPSLGKRWG 191 >Q5KUZ7.1 Q5KUZ7_GEOKA Fructokinases EC=2.7.1.4 Length = 290 Score = 261 bits (666), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 121/190 (63%), Positives = 142/190 (74%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGP 62 +LGAIEAGGTKFVC IG+E G I +R FPTT PEETM V+D+F +EAIGIGSFGP Sbjct: 2 ILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDFFRPHRIEAIGIGSFGP 61 Query: 63 IDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGL 122 +D SPTYGYIT+TPK W Q++ VGT+ HF VP+GFDTDVN AALGE +WGAAQGL Sbjct: 62 VDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTDVNAAALGELRWGAAQGL 121 Query: 123 DSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAA 182 DSCLY +L+HG+ HPEMGH+ VRRHP+D F G CPYH DCLEG+A+ Sbjct: 122 DSCLYMTVGTGIGVGAVVEGRLLHGMLHPEMGHMFVRRHPDDAFAGICPYHGDCLEGMAS 181 Query: 183 GPAIGKRWGK 192 GPAI +RWGK Sbjct: 182 GPAIERRWGK 191 >C3J4H9.1 C3J4H9_9BACI ROK family protein Length = 290 Score = 261 bits (666), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 122/190 (64%), Positives = 143/190 (75%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGP 62 +LGAIEAGGTKFVC IG+E G I +R FPTT PEETM V+D+F +EAIGIGSFGP Sbjct: 2 ILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDFFRPHRIEAIGIGSFGP 61 Query: 63 IDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGL 122 +D SPTYGYIT+TPK W Q++ VGT+ HF VP+GFDTDVN AALGE +WGAA+GL Sbjct: 62 VDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTDVNAAALGEQRWGAARGL 121 Query: 123 DSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAA 182 DSCLY +L+HGL HPEMGH+LVRRHP+D F G CPYH DCLEG+A+ Sbjct: 122 DSCLYMTVGTGIGVGAIIEGRLLHGLLHPEMGHMLVRRHPDDAFAGICPYHGDCLEGMAS 181 Query: 183 GPAIGKRWGK 192 GPAI +RWGK Sbjct: 182 GPAIERRWGK 191 >B7YAV9.1 B7YAV9_9BACI ROK family protein Length = 290 Score = 261 bits (666), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 122/190 (64%), Positives = 143/190 (75%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGP 62 +LGAIEAGGTKFVC IG+E G I +R FPTT PEETM V+D+F +EAIGIGSFGP Sbjct: 2 ILGAIEAGGTKFVCAIGDEQGNIHERAVFPTTAPEETMAHVIDFFRPHRIEAIGIGSFGP 61 Query: 63 IDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGL 122 +D SPTYGYIT+TPK W Q++ VGT+ HF VP+GFDTDVN AALGE +WGAA+GL Sbjct: 62 VDLRPDSPTYGYITSTPKQAWAQFDFVGTMKQHFPVPIGFDTDVNAAALGEQRWGAARGL 121 Query: 123 DSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAA 182 DSCLY +L+HGL HPEMGH+LVRRHP+D F G CPYH DCLEG+A+ Sbjct: 122 DSCLYMTVGTGIGVGAIIEGRLLHGLLHPEMGHMLVRRHPDDAFAGICPYHGDCLEGMAS 181 Query: 183 GPAIGKRWGK 192 GPAI +RWGK Sbjct: 182 GPAIERRWGK 191 >A4IT14.1 A4IT14_GEOTN Fructokinase Length = 290 Score = 252 bits (644), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 120/199 (60%), Positives = 143/199 (71%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGP 62 +LGAIEAGGTKFVC +G+E G I R FPTT PEETM V+++F +EAIG+GSFGP Sbjct: 2 ILGAIEAGGTKFVCAVGDEHGRIHKREVFPTTVPEETMANVIEFFRPHGIEAIGVGSFGP 61 Query: 63 IDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGL 122 ID SPTYGYIT+TPK W ++ VGT+ +F VP+GFDTDVN AALGE +WGAAQGL Sbjct: 62 IDLRPSSPTYGYITSTPKQAWTNFDFVGTLKQYFPVPIGFDTDVNAAALGELRWGAAQGL 121 Query: 123 DSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAA 182 DSCLY +L+HGL HPEMGHI+VRRHP+D F G CPYH DCLEG+A+ Sbjct: 122 DSCLYMTVGTGIGVGAVVEGRLLHGLLHPEMGHIVVRRHPDDAFAGICPYHGDCLEGMAS 181 Query: 183 GPAIGKRWGKXXXXGKSRQ 201 GPAI +RWGK +R Sbjct: 182 GPAIERRWGKKGAELAARN 200 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.142 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,432,962,047 Number of extensions: 59377063 Number of successful extensions: 104086 Number of sequences better than 1.0e-05: 490 Number of HSP's gapped: 103780 Number of HSP's successfully gapped: 490 Length of query: 221 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 90 Effective length of database: 1,826,543,238 Effective search space: 164388891420 Effective search space used: 164388891420 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)