BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0041.p (314 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3JU68.1 B3JU68_9DELT 1-phosphofructokinase 342 3e-92 B5E9Q7.1 B5E9Q7_GEOBB 1-phosphofructokinase 338 4e-91 B2HJV9.1 B2HJV9_MYCMM Phosphofructokinase, PfkB_1 290 1e-76 C1U5K8.1 C1U5K8_9SPHI 1-phosphofructokinase 289 2e-76 B4REC3.1 B4REC3_PHEZH Putative 6-phosphofructokinase 288 6e-76 >B3JU68.1 B3JU68_9DELT 1-phosphofructokinase Length = 311 Score = 342 bits (876), Expect = 3e-92, Method: Compositional matrix adjust. Identities = 169/304 (55%), Positives = 219/304 (72%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 IVT+T+NP++D S +D VV + KL C E ++PGGGG+NVSRA+ +LGGESLALY SGG Sbjct: 4 IVTITMNPAIDKSATVDHVVAERKLYCSEPLFEPGGGGINVSRAVRKLGGESLALYPSGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 GQ L +LEQEGV H+ + ++G TRENLIV+E +TGQQ+RF MPGP +WQ CLE Sbjct: 64 HSGQTLRLLLEQEGVAHRLVAVAGWTRENLIVLESATGQQYRFGMPGPDLSATEWQACLE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAGVYL 181 Q+ + G KP YIVASGSLPPG P DFY RV K ARV+VD SGE ++ A GV+L Sbjct: 124 QIFAGGAKPDYIVASGSLPPGVPPDFYARVARLGKQHGARVVVDASGEPMRLALREGVFL 183 Query: 182 LKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLKA 241 +KPN RE E+ G +A+V A A +++ G +EVVV+SLG G L+++ G E L Sbjct: 184 IKPNIREFREMIGEGGEDEAKVLALAKKIVLDGESEVVVISLGAAGVLMVSDSGVERLHP 243 Query: 242 PEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAERL 301 P VP++S VGAGDS+V G+V SL RG LR AV+FG+A+G AAVM P +LC+REDAERL Sbjct: 244 PTVPIISKVGAGDSMVAGMVLSLARGNGLRDAVRFGVAAGTAAVMTPGTELCRREDAERL 303 Query: 302 FKQI 305 ++Q+ Sbjct: 304 YRQM 307 >B5E9Q7.1 B5E9Q7_GEOBB 1-phosphofructokinase Length = 311 Score = 338 bits (867), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 165/304 (54%), Positives = 219/304 (72%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 IVT+T+NP++D S +D VV + KL C E ++PGGGG+NVSRA+ +LGGESLALY SGG Sbjct: 4 IVTITMNPAIDKSATVDHVVAERKLYCSEPLFEPGGGGINVSRAVRKLGGESLALYPSGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 GQ L +L+QEG+ H+ I ++G TRENLIV+E +TGQQ+RF MPGP +WQ CLE Sbjct: 64 HSGQTLRLLLDQEGLTHRPIEVAGWTRENLIVLESATGQQYRFGMPGPDLSAKEWQACLE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAGVYL 181 Q+ + G +P Y+VASGSLPPG P DFY R+ K ARV+VD SGE ++ A GV+L Sbjct: 124 QIFAGGARPDYLVASGSLPPGVPQDFYARIARLGKQHGARVVVDASGEPMRLALREGVFL 183 Query: 182 LKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLKA 241 +KPN RE E+ G +A V A A +++ G++EVVV+SLG G L+++ G E L Sbjct: 184 IKPNIREFREMIGEGGEDEARVLALAKKIVLDGQSEVVVISLGAAGVLMVSDSGVERLHP 243 Query: 242 PEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAERL 301 P VP++S VGAGDS+V G+V SL RG LR AV+FG+A+G AAVM P +LC+REDAERL Sbjct: 244 PTVPIISKVGAGDSMVAGMVLSLARGNTLRDAVRFGVAAGTAAVMTPGTELCRREDAERL 303 Query: 302 FKQI 305 ++Q+ Sbjct: 304 YRQM 307 >B2HJV9.1 B2HJV9_MYCMM Phosphofructokinase, PfkB_1 Length = 323 Score = 290 bits (742), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 152/304 (50%), Positives = 198/304 (65%), Gaps = 1/304 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 IVTLT+NP++D +T + V P KLRC E Y PGGGG+NV+R H LGG A++ GG Sbjct: 14 IVTLTMNPALDITTCVGVVRPTEKLRCSETRYDPGGGGINVARIAHVLGGSVFAVFPVGG 73 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 HG + ++L + GV EIPI+ TRE+ V E STG+Q+RF +PGP + QCL+ Sbjct: 74 SHGAHVSKLLSEAGVAFGEIPIAASTRESFTVNESSTGEQYRFVLPGPVLAPEEQAQCLQ 133 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAGVYL 181 +L ++VASGSLPPG PADFY RV EA + AR+I+DTSG L + AGVYL Sbjct: 134 ELGFAARSAQFVVASGSLPPGVPADFYQRVAEACRGLGARLILDTSGGGLSHIS-AGVYL 192 Query: 182 LKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLKA 241 LK + REL E G + +AE AAA EL+E GR EVVVVSLG +GALL TR +A Sbjct: 193 LKASVRELRECVGRPLVTEAEQLAAASELVETGRAEVVVVSLGSDGALLATRRARHRFRA 252 Query: 242 PEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAERL 301 E+ S VGAGD++V + L RGWPL ++V+ GIA+GAA ++ P Q C+R D ERL Sbjct: 253 VEMRGGSGVGAGDAMVAAITVGLARGWPLVKSVRLGIAAGAAMLLTPGTQACERADVERL 312 Query: 302 FKQI 305 F+ + Sbjct: 313 FEHV 316 >C1U5K8.1 C1U5K8_9SPHI 1-phosphofructokinase Length = 319 Score = 289 bits (740), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 145/305 (47%), Positives = 202/305 (66%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 IVTLT+NP++D S+ + R+VPD+KLRC +++ GGGG+NV++AIHRLGG +A++ GG Sbjct: 6 IVTLTINPALDKSSNVPRIVPDNKLRCETPNFEAGGGGINVAKAIHRLGGNPIAIFTVGG 65 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 GQ LH++++ EGV I TREN V+E STG Q+RF MPG + + + LE Sbjct: 66 PTGQRLHKLVKSEGVETMVIETKQWTRENFHVLETSTGFQYRFGMPGTEMMPTEVRSILE 125 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAGVYL 181 ++ L +P+YI+ASGSLPPG P+DFYG++ AR I DTSG+AL+ A +AGVYL Sbjct: 126 TMEELDPRPSYIIASGSLPPGVPSDFYGQIARIAHAQGARFIADTSGDALRYATEAGVYL 185 Query: 182 LKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLKA 241 LKPN EL EL G EV AA LI +G+ E+V+VS+G GA+L+T + EH+ A Sbjct: 186 LKPNITELSELVGVDKLEMDEVDDAARALIIRGQCEIVIVSMGAMGAMLVTEDQVEHIPA 245 Query: 242 PEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAERL 301 P V S VGAGDS++ G+V++L +G R AV+ G+A G AA MN +L + +D RL Sbjct: 246 PPVQPKSTVGAGDSMLAGIVWTLSQGKSYREAVRMGVACGTAATMNSGSELFRMQDVNRL 305 Query: 302 FKQIE 306 + Sbjct: 306 LDWLN 310 >B4REC3.1 B4REC3_PHEZH Putative 6-phosphofructokinase Length = 320 Score = 288 bits (736), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 148/307 (48%), Positives = 197/307 (64%), Gaps = 4/307 (1%) Query: 1 MIVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASG 60 ++VTLT+NP++D ST RV PD KLRC PGGGG+NV+R HRLG +LA++ SG Sbjct: 3 VVVTLTLNPALDVSTSTPRVEPDRKLRCGAGRRDPGGGGINVARVAHRLGARTLAIFPSG 62 Query: 61 GLHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCL 120 GL GQ+L ++E EGVG +P+SG+TRE++ V++ES+G Q+RF MPGPH +W CL Sbjct: 63 GLTGQMLERLVEAEGVGSLPVPVSGETREDVTVLDESSGAQYRFIMPGPHLHGVEWMACL 122 Query: 121 EQLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAGVY 180 + L L KP ++ ASGSLPPG P D Y RV E V+ W R +DTSG LQ A V+ Sbjct: 123 KALAGLEAKPDFVCASGSLPPGAPDDLYARVAEIVESWGVRFALDTSGPPLQAALSERVF 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN REL EL G + +A AA LI +GRT V ++LG EGALL+T + + + Sbjct: 183 LIKPNLRELRELTGAPLEDEASRIAACRSLIARGRTAAVALTLGHEGALLVTAQ--DAWR 240 Query: 241 APEVPV--LSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDA 298 AP +PV +S VGAGDS +G +V+ L LR A F +A G+AA++ QL D Sbjct: 241 APALPVRPVSSVGAGDSFLGAMVWGLASKLTLREAFAFAMAGGSAALLAQGTQLAGARDV 300 Query: 299 ERLFKQI 305 RL Q+ Sbjct: 301 RRLLPQV 307 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,940,480,023 Number of extensions: 85596755 Number of successful extensions: 248823 Number of sequences better than 1.0e-05: 1636 Number of HSP's gapped: 247104 Number of HSP's successfully gapped: 1636 Length of query: 314 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 179 Effective length of database: 1,788,859,178 Effective search space: 320205792862 Effective search space used: 320205792862 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)