BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0040.p (314 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB56.1 B3KB56_9BACI Magnesium and cobalt transport protein CorA 558 e-157 B1DDJ9.1 B1DDJ9_9BACL Magnesium and cobalt transport protein CorA 500 e-140 C0ZKY1.1 C0ZKY1_BREBN Putative uncharacterized protein 377 e-102 Q2RLT7.1 Q2RLT7_MOOTA Magnesium and cobalt transport protein CorA 200 1e-49 A8FB19.1 A8FB19_BACP2 MIT family metal ion transporter CorA 199 2e-49 >B3KB56.1 B3KB56_9BACI Magnesium and cobalt transport protein CorA Length = 311 Score = 558 bits (1438), Expect = e-157, Method: Compositional matrix adjust. Identities = 270/311 (86%), Positives = 290/311 (93%) Query: 4 MKIRLVNAGVFTPVDDIEETLTAPTEGFYWIDADVEDLELLQPLYNLHDLAVEDCLSEED 63 MKIRLV++GVFTPVDDIE TLTAP GFYW+DADV+DL LQPL++LHDLAVEDCLSEE+ Sbjct: 1 MKIRLVDSGVFTPVDDIEMTLTAPASGFYWLDADVDDLAELQPLFSLHDLAVEDCLSEEE 60 Query: 64 QRPKLEIYESHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWE 123 QRPK+EIYE+HYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELR +KP+LWE Sbjct: 61 QRPKIEIYENHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRTVKPLLWE 120 Query: 124 QEVSEPDRFLYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILW 183 EVS PDRFLYLLIDLV DNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILW Sbjct: 121 DEVSTPDRFLYLLIDLVTDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILW 180 Query: 184 LKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQ 243 LKK+LGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQ Sbjct: 181 LKKVLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQ 240 Query: 244 SSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCG 303 SSIANRANEIMRVFTAITTIF+PLT+ITGIYGMNF+ +PE H +Y YY VI +MV G Sbjct: 241 SSIANRANEIMRVFTAITTIFIPLTLITGIYGMNFEYMPELHWRYSYYVVIGIMVFAGAL 300 Query: 304 MLVVFRKKDWL 314 M +FRKKDW+ Sbjct: 301 MFYLFRKKDWI 311 >B1DDJ9.1 B1DDJ9_9BACL Magnesium and cobalt transport protein CorA Length = 311 Score = 500 bits (1287), Expect = e-140, Method: Compositional matrix adjust. Identities = 237/311 (76%), Positives = 277/311 (89%) Query: 4 MKIRLVNAGVFTPVDDIEETLTAPTEGFYWIDADVEDLELLQPLYNLHDLAVEDCLSEED 63 MKIR VN G+FT ++DI ETL EGFYWIDADVEDL +LQPL+ +HDLAVEDCLSEE+ Sbjct: 1 MKIRHVNNGLFTHIEDINETLVPAAEGFYWIDADVEDLAILQPLFGMHDLAVEDCLSEEE 60 Query: 64 QRPKLEIYESHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWE 123 QRPK+EIYE+ YFIV+NSIRFDDEEIFLRALN+FLGRH+IIT+TKQK++ELR LKP+LWE Sbjct: 61 QRPKIEIYENQYFIVINSIRFDDEEIFLRALNIFLGRHYIITITKQKLNELRTLKPVLWE 120 Query: 124 QEVSEPDRFLYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILW 183 QEV+ PD FLY L+D+VVDNYF VGDRI+ RIE LEEDILM+TKK+HL+EIIGLR EILW Sbjct: 121 QEVNRPDYFLYHLVDIVVDNYFLVGDRIDNRIEMLEEDILMNTKKTHLNEIIGLRGEILW 180 Query: 184 LKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQ 243 LKK+LGPQK++I TLNK+DL+LIDDQLQKYFSDIYENAVKI+E+F+TYR+LMGNLREAYQ Sbjct: 181 LKKVLGPQKDLIATLNKRDLKLIDDQLQKYFSDIYENAVKIAESFDTYRELMGNLREAYQ 240 Query: 244 SSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCG 303 S+I+ RANEIMRVFTA+TTIFMPLT ITGIYGMNFD IP H + G ++ +M+ LG G Sbjct: 241 STISARANEIMRVFTAMTTIFMPLTFITGIYGMNFDFIPGMHVRGGSIILLGIMLALGVG 300 Query: 304 MLVVFRKKDWL 314 MLVVFRKK WL Sbjct: 301 MLVVFRKKKWL 311 >C0ZKY1.1 C0ZKY1_BREBN Putative uncharacterized protein Length = 311 Score = 377 bits (967), Expect = e-102, Method: Compositional matrix adjust. Identities = 185/311 (59%), Positives = 239/311 (76%) Query: 4 MKIRLVNAGVFTPVDDIEETLTAPTEGFYWIDADVEDLELLQPLYNLHDLAVEDCLSEED 63 MKIRLV G+ ++DIEE T P GFYWI A DL++LQPL+ LH LAVEDC+ EE+ Sbjct: 1 MKIRLVQNGIINEIEDIEEAATPPVNGFYWIHATPFDLDILQPLFGLHHLAVEDCIDEEE 60 Query: 64 QRPKLEIYESHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWE 123 QRPKL+ YE HYF+V+N I F + +IFLR +N+FLG IITVTKQ+ E + I+ E Sbjct: 61 QRPKLQNYEDHYFMVINGITFHNHDIFLREINIFLGSSTIITVTKQEAPEFTTMLSIIRE 120 Query: 124 QEVSEPDRFLYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILW 183 +EV+ PD FLY L+D +VD YF V ++IE +E LEE ILMHTKKSHL +IIGLRSEIL+ Sbjct: 121 KEVNRPDTFLYYLVDQIVDLYFDVTEKIEDLLEDLEEQILMHTKKSHLDQIIGLRSEILY 180 Query: 184 LKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQ 243 +KML PQ+++I+ L+KK+L LI L+KYF DI ++A KI E+FE +R+L+GNLREAYQ Sbjct: 181 ARKMLVPQRDLIDALHKKELPLIQPYLRKYFGDIVDDASKIVESFEAFRELIGNLREAYQ 240 Query: 244 SSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCG 303 +++A RAN+IMRVFTA+TTIFMPLT++TGIYGMNFD +PE S YGYY V+ M +G Sbjct: 241 AALAGRANDIMRVFTALTTIFMPLTIVTGIYGMNFDVMPELRSPYGYYIVLGCMAVIGTT 300 Query: 304 MLVVFRKKDWL 314 M ++F+KKDWL Sbjct: 301 MFIIFKKKDWL 311 >Q2RLT7.1 Q2RLT7_MOOTA Magnesium and cobalt transport protein CorA Length = 316 Score = 200 bits (509), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 118/317 (37%), Positives = 179/317 (56%), Gaps = 21/317 (6%) Query: 13 VFTPVDDIEETLTA-----PTEGFYWID---ADVEDLELLQPLYNLHDLAVEDCLSEEDQ 64 +F P ++E L +E F W+D E+L+LL L++ H LAV+DCL Sbjct: 6 IFHPKQGLKENLPPGFQPRQSEEFTWLDLVQPAREELDLLADLFHFHPLAVKDCLHPH-Y 64 Query: 65 RPKLEIYESHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWEQ 124 RP + Y+ + F+ + I+ + ALNVFLG FI+TV ++ EL L I W Q Sbjct: 65 RPGMAQYDEYAFLTLRCIQARQRSL-TAALNVFLGPGFIVTVHQE---ELDFLNKI-WHQ 119 Query: 125 EVSEPDRF-------LYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGL 177 +P F LY L++ + + +F+ DR EARIE LEE++ + L+++ + Sbjct: 120 YRQDPRLFAKGVDFILYSLLEPLTETFFAFFDRTEARIEYLEEEVFRQPTRRILNQVFTM 179 Query: 178 RSEILWLKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGN 237 R + + L+K+LG Q+++IN L + L+ + + +F DIY +++ + ET+ DL + Sbjct: 180 RRQAIKLRKILGAQRDIINVLAYPEFNLVKPENRVFFLDIYNEMLRLIDQVETFHDLASS 239 Query: 238 LREAYQSSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVM 297 E Y S +NR NEIM+V T ITTI MPL++I GIYGMNF +PE +YGY+ V+ VM Sbjct: 240 TLEIYLSLTSNRLNEIMKVLTVITTIMMPLSLIAGIYGMNFRYMPELEWRYGYFVVLGVM 299 Query: 298 VTLGCGMLVVFRKKDWL 314 L ML FR+K W Sbjct: 300 ALLAGVMLYFFRRKGWF 316 >A8FB19.1 A8FB19_BACP2 MIT family metal ion transporter CorA Length = 319 Score = 199 bits (507), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 105/299 (35%), Positives = 187/299 (62%), Gaps = 9/299 (3%) Query: 22 ETLTAPTEGFYWIDADV---EDLELLQPLYNLHDLAVEDCLSEEDQRPKLEIYESHYFIV 78 + L++P +YWID ++ LL+ + H LA+EDC QRPKL+ YE + F V Sbjct: 20 QQLSSPDIAWYWIDFHAPTEKEAALLKEYFQFHPLAIEDCFHYL-QRPKLDFYEGYLFFV 78 Query: 79 VNSIRFDDEEIFLRALNVFLGRHFIITV-TKQKIHELRVLKPILWEQEV--SEPDRFLYL 135 ++++ + + + +++F+G ++I+T K+ + RV++ + + S P+ Y+ Sbjct: 79 LHAL--NQKTLRSEEIDLFVGDNYIVTFHLKEAPYIDRVIRKLKESDKARNSGPEHIAYM 136 Query: 136 LIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILWLKKMLGPQKEVI 195 LID +VD+YF + +IE R+ ++E++ T + ++E+ G+RS++L L++ + P ++++ Sbjct: 137 LIDQLVDDYFPIIYQIEDRLNEIEDEEGHKTYGTLMNEVFGIRSDLLKLRRTIIPMRDLL 196 Query: 196 NTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQSSIANRANEIMR 255 + ++ D Q YF+DIY++ +K++E E+ RD+ +LR++YQ+ +NR N IM Sbjct: 197 YRIVNTNMMRSDANRQAYFNDIYDHLLKLTEIVESNRDMTADLRDSYQTLNSNRMNAIMM 256 Query: 256 VFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCGMLVVFRKKDWL 314 T ++TIF+PLT I G+YGMNFDN+PE H +YGY+GV+ M L GML+ F+ K W Sbjct: 257 TLTIVSTIFIPLTFIVGVYGMNFDNMPELHWKYGYFGVLIFMGLLVSGMLLWFKHKGWF 315 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.141 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,846,063,887 Number of extensions: 76558203 Number of successful extensions: 228749 Number of sequences better than 1.0e-05: 1929 Number of HSP's gapped: 227157 Number of HSP's successfully gapped: 1931 Length of query: 314 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 179 Effective length of database: 1,788,859,178 Effective search space: 320205792862 Effective search space used: 320205792862 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)