BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0038.p (399 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB58.1 B3KB58_9BACI Cystathionine gamma-synthase EC=2.5.1.48 664 0.0 B1DDJ7.1 B1DDJ7_9BACL Cystathionine gamma-synthase EC=2.5.1.48 621 e-176 C0ZGE6.1 C0ZGE6_BREBN Cystathionine gamma-synthase/O-acetylhomos... 520 e-146 C1PQ83.1 C1PQ83_ALIAC Cystathionine beta-lyase/cystathionine gam... 468 e-130 B7DMN1.1 B7DMN1_9BACL Cystathionine gamma-synthase EC=2.5.1.48 466 e-129 >B3KB58.1 B3KB58_9BACI Cystathionine gamma-synthase EC=2.5.1.48 Length = 394 Score = 664 bits (1713), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/374 (82%), Positives = 350/374 (93%), Gaps = 1/374 (0%) Query: 1 MDEK-LRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVL 59 M++K L IESRLAQIGS ++P TGA+N+PIY ATA+RHP+LGQSTGFDY RTKNPTRSVL Sbjct: 1 MEKKPLNIESRLAQIGSVQEPVTGAVNFPIYQATAYRHPKLGQSTGFDYARTKNPTRSVL 60 Query: 60 EEAVAELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGIS 119 E+A AELESGDA FACSSGMAAL T+FALFGQGDHL+VSLDLYGGTYRLLERI+SK+G++ Sbjct: 61 EDACAELESGDAGFACSSGMAALQTIFALFGQGDHLIVSLDLYGGTYRLLERIMSKFGVT 120 Query: 120 ASYVDTNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLT 179 A+YVDTNDLD LE SRRPNTKAVFIETPTNPLMMITDIEAVCTW+RRH L++IVDNTLLT Sbjct: 121 ATYVDTNDLDALEGSRRPNTKAVFIETPTNPLMMITDIEAVCTWARRHQLISIVDNTLLT 180 Query: 180 PFFQRPLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDS 239 P+FQRP+ELGADI++HSATKYLGGHNDVLAG+IVTKG ELS EMA LHNS+GAVL+P+DS Sbjct: 181 PYFQRPIELGADIVIHSATKYLGGHNDVLAGIIVTKGKELSEEMAFLHNSIGAVLSPSDS 240 Query: 240 YQLMKGMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTG 299 YQLM+GMKTLALRM+RHESNA A+A +L HPAI EV++P LPDHPG++IQ RQSSGNTG Sbjct: 241 YQLMRGMKTLALRMDRHESNATAVATFLTTHPAIGEVYYPALPDHPGHEIQNRQSSGNTG 300 Query: 300 IFSFKVKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRL 359 IFSFKVKDA YVEP+LRHI+LIAFAESLGGVESLMTYPAVQTH DIP+EIR+A+GVDDRL Sbjct: 301 IFSFKVKDARYVEPVLRHIKLIAFAESLGGVESLMTYPAVQTHTDIPLEIREAIGVDDRL 360 Query: 360 LRFSVGIEHADDLI 373 LRFSVGIEHADDLI Sbjct: 361 LRFSVGIEHADDLI 374 >B1DDJ7.1 B1DDJ7_9BACL Cystathionine gamma-synthase EC=2.5.1.48 Length = 386 Score = 621 bits (1601), Expect = e-176, Method: Compositional matrix adjust. Identities = 289/369 (78%), Positives = 333/369 (90%) Query: 5 LRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVA 64 ++IES LA IGS+++P TGA+++PIY ATAFRHP+LGQSTGFDY RTK+PTR+VLEEA A Sbjct: 1 MKIESHLAHIGSEKEPVTGAVSFPIYQATAFRHPKLGQSTGFDYARTKSPTRTVLEEAAA 60 Query: 65 ELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVD 124 +LESGDA FACSSGMAALTT+FALF QGDHL+VSLDLYGGTYRLLE+I+S+YG++ASYVD Sbjct: 61 QLESGDAGFACSSGMAALTTIFALFSQGDHLIVSLDLYGGTYRLLEKIMSRYGVTASYVD 120 Query: 125 TNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQR 184 TNDLDG+ +PNTKAV IETPTNPLMMITD+E V W++ GLLTIVDNTLLTPFFQR Sbjct: 121 TNDLDGMSELLQPNTKAVLIETPTNPLMMITDLERVSAWAKSKGLLTIVDNTLLTPFFQR 180 Query: 185 PLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMK 244 P+ELGADI+VHSATKYLGGHNDVLAGLIVTKG ELS ++A LHNSLGAVL P DS+ LM+ Sbjct: 181 PIELGADIVVHSATKYLGGHNDVLAGLIVTKGKELSEQIAFLHNSLGAVLGPQDSWLLMR 240 Query: 245 GMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFK 304 GMKTLALRMERH+ NA A+A +LLEH AI +V++P LP HPG+D+Q +QSSGNTGIFSF+ Sbjct: 241 GMKTLALRMERHQLNATAIANFLLEHEAIEDVYYPALPHHPGHDVQNKQSSGNTGIFSFR 300 Query: 305 VKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSV 364 +KDA YVEP+LRHI+LIAFAESLGGVESLMTYPAVQTHADIP EIR +GVDDRLLRFSV Sbjct: 301 LKDARYVEPILRHIQLIAFAESLGGVESLMTYPAVQTHADIPAEIRQKIGVDDRLLRFSV 360 Query: 365 GIEHADDLI 373 GIEHADDLI Sbjct: 361 GIEHADDLI 369 >C0ZGE6.1 C0ZGE6_BREBN Cystathionine gamma-synthase/O-acetylhomoserine aminocarboxypropyltransferase EC=2.5.1.48 EC=2.5.1.49 Length = 387 Score = 520 bits (1340), Expect = e-146, Method: Compositional matrix adjust. Identities = 242/374 (64%), Positives = 308/374 (82%) Query: 5 LRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVA 64 +++E++L Q G +D TGA+++P+Y ATA+RHP LGQSTG+DY RT NPTR++LEE +A Sbjct: 1 MKLETKLIQAGVGKDEKTGAVSFPVYYATAYRHPALGQSTGYDYTRTANPTRTILEETIA 60 Query: 65 ELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVD 124 ESGDA FAC+SGMAA+ TV LF QGDHL+VSLDLYGGTYRL E++LS+YG++ SYVD Sbjct: 61 IAESGDAGFACASGMAAVHTVMGLFSQGDHLIVSLDLYGGTYRLFEQVLSRYGLTFSYVD 120 Query: 125 TNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQR 184 D+ L+ + P+TKA+F+ETPTNPLM ITDI AV +++H LLTIVDNT LTP+ QR Sbjct: 121 LRDIVALKNAICPDTKAIFVETPTNPLMQITDIRAVAQLAKQHSLLTIVDNTFLTPYCQR 180 Query: 185 PLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMK 244 PLELGADI++HSATKYL GHNDVLAGLIVTKG ELS ++ LHNS+GAVL P D + L++ Sbjct: 181 PLELGADIVLHSATKYLAGHNDVLAGLIVTKGEELSEKIRFLHNSIGAVLGPQDCWLLIR 240 Query: 245 GMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFK 304 GMKTLALRMERH+SNAL +A L EHPA+AEVF+PGLP+HPG++IQ Q++G++G+ SF+ Sbjct: 241 GMKTLALRMERHQSNALIVAEKLREHPAVAEVFYPGLPEHPGHEIQVNQATGHSGMVSFR 300 Query: 305 VKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSV 364 V+D V L+++++++FAESLGGVESL TYPA QTHADIP E+R+ VGV RLLR SV Sbjct: 301 VRDVEQVGLFLQNLQIVSFAESLGGVESLCTYPATQTHADIPQEVREHVGVCYRLLRLSV 360 Query: 365 GIEHADDLIADLGQ 378 GIEH DD++ADL Q Sbjct: 361 GIEHPDDVVADLFQ 374 >C1PQ83.1 C1PQ83_ALIAC Cystathionine beta-lyase/cystathionine gamma-synthase EC=4.4.1.1 Length = 388 Score = 468 bits (1205), Expect = e-130, Method: Compositional matrix adjust. Identities = 227/374 (60%), Positives = 287/374 (76%) Query: 5 LRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVA 64 ++IES+LAQIG+++DP TGA++ PIY++T + HP LGQSTGFDY RT NPTR +LE+A+A Sbjct: 1 MKIESKLAQIGNRQDPGTGAVSAPIYHSTTYAHPALGQSTGFDYTRTLNPTRKILEDAIA 60 Query: 65 ELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVD 124 +LE G FA SSGMAA+ VF+LF GDH++VS DLYGGTYR+LE I GI A+YV Sbjct: 61 DLEGGVRGFAFSSGMAAVHAVFSLFSPGDHVIVSSDLYGGTYRVLEHIFRPLGIEATYVH 120 Query: 125 TNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQR 184 T L+ +EA+ R T+A+FIETPTNP M ITDI A ++RR G LTIVDNT +TP+FQR Sbjct: 121 TGHLEEVEAAARDTTRALFIETPTNPTMQITDIAAASLFARRRGWLTIVDNTFMTPYFQR 180 Query: 185 PLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMK 244 P+ELGADI+VHSATK+LGGHNDVLAGL+V K E++ + + NS+GAVL P D++ LM+ Sbjct: 181 PIELGADIVVHSATKFLGGHNDVLAGLVVAKSDEIAERLYFVQNSIGAVLGPQDAWLLMR 240 Query: 245 GMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFK 304 GMKTLA+RMER NA A+A +L I +V++PGL HPG I +Q+SG G+ SF+ Sbjct: 241 GMKTLAIRMERCAMNASAIAAWLDARDDIVKVYYPGLDTHPGKSICSKQASGFGGMVSFE 300 Query: 305 VKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSV 364 V DA V +L H++L+ FAESLGGVE+L+TYP+ QTHADIP EIR+ VGV DRLLR SV Sbjct: 301 VLDARMVPFILEHLKLVTFAESLGGVETLITYPSRQTHADIPEEIREKVGVTDRLLRLSV 360 Query: 365 GIEHADDLIADLGQ 378 GIEH DLI DL Q Sbjct: 361 GIEHIGDLIDDLSQ 374 >B7DMN1.1 B7DMN1_9BACL Cystathionine gamma-synthase EC=2.5.1.48 Length = 388 Score = 466 bits (1199), Expect = e-129, Method: Compositional matrix adjust. Identities = 225/374 (60%), Positives = 286/374 (76%) Query: 5 LRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVA 64 ++IES+LAQIG+++DP TGA++ PIY++T + HP LGQSTGFDY RT NPTR +LE+A+A Sbjct: 1 MKIESKLAQIGNRQDPRTGAVSAPIYHSTTYAHPALGQSTGFDYTRTLNPTRKILEDAIA 60 Query: 65 ELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVD 124 +LE G FA SSGMAA+ VF+LF GDH++VS DLYGGTYR+LE I GI A+YV Sbjct: 61 DLEGGVRGFAFSSGMAAVHAVFSLFSPGDHVIVSSDLYGGTYRVLEHIFRPLGIEATYVH 120 Query: 125 TNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQR 184 T LD +EA+ R T+A+FIETPTNP M ITDI A ++RR G LTIVDNT +TP+FQR Sbjct: 121 TGHLDEVEAAARDTTRALFIETPTNPTMQITDIAAASLFARRRGWLTIVDNTFMTPYFQR 180 Query: 185 PLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMK 244 P+ELGAD++VHSATK+LGGHNDVLAGL+V + E++ + + NS+GAVL P D++ LM+ Sbjct: 181 PIELGADVVVHSATKFLGGHNDVLAGLVVARSDEIAERLYFVQNSIGAVLGPQDAWLLMR 240 Query: 245 GMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFK 304 GMKTLA+RMER NA A+A +L I +V++PGL HPG + +Q+SG G+ SF+ Sbjct: 241 GMKTLAIRMERCAMNASAIAAWLDARDDIVKVYYPGLDTHPGKSVCAKQASGFGGMVSFE 300 Query: 305 VKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSV 364 V DA V +L H++L+ FAESLGGVE+L+TYP QTHADIP EIR+ VGV DRLLR SV Sbjct: 301 VLDARMVPFILEHLKLVTFAESLGGVETLITYPTRQTHADIPEEIREKVGVTDRLLRLSV 360 Query: 365 GIEHADDLIADLGQ 378 GIEH DLI DL Q Sbjct: 361 GIEHIGDLIDDLSQ 374 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,254,887,966 Number of extensions: 93260646 Number of successful extensions: 236586 Number of sequences better than 1.0e-05: 4546 Number of HSP's gapped: 224963 Number of HSP's successfully gapped: 5571 Length of query: 399 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 261 Effective length of database: 1,760,596,133 Effective search space: 459515590713 Effective search space used: 459515590713 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)