BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0036.p (255 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB60.1 B3KB60_9BACI Radical SAM domain protein 427 e-118 B1DDJ5.1 B1DDJ5_9BACL Radical SAM domain protein 392 e-107 A8VX44.1 A8VX44_9BACI Radical SAM domain protein 343 1e-92 C0Z9A9.1 C0Z9A9_BREBN Putative menaquinone biosynthesis protein 341 4e-92 Q9K864.1 Q9K864_BACHD BH3143 protein 338 2e-91 >B3KB60.1 B3KB60_9BACI Radical SAM domain protein Length = 377 Score = 427 bits (1098), Expect = e-118, Method: Compositional matrix adjust. Identities = 197/244 (80%), Positives = 225/244 (92%), Gaps = 1/244 (0%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 ITMHSFSPAEIMKMVE+SGL LE+V+RE+H+AGLDSLPGGGAEILDDR R+K+SRLKG+W Sbjct: 135 ITMHSFSPAEIMKMVEISGLTLEEVMRELHSAGLDSLPGGGAEILDDRIRKKVSRLKGTW 194 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 R+WMDVMQTAHK+GMNTTATMVIGLGE+MEERALH+LRVR+AQDECI N YDS+GFLAFI Sbjct: 195 RQWMDVMQTAHKVGMNTTATMVIGLGETMEERALHMLRVRDAQDECIQNGYDSKGFLAFI 254 Query: 132 SWTFQPDNTNLKLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGCND 191 +TFQPDNTNLK +R+TPE YLKTVAI RL +DN+ N QSSWVTMGPE+GKLSL YGCND Sbjct: 255 PFTFQPDNTNLKRERETPEAYLKTVAIGRLAIDNVPNLQSSWVTMGPEIGKLSLSYGCND 314 Query: 192 FGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKIDNDF 251 FGSTM+EENVVSSAGA +KVNIESI Q+IRE G IPAQRNTRYDILRVF+D ++ ++ DF Sbjct: 315 FGSTMMEENVVSSAGAVYKVNIESIIQLIRETGNIPAQRNTRYDILRVFED-NSTVERDF 373 Query: 252 VMQN 255 VMQN Sbjct: 374 VMQN 377 >B1DDJ5.1 B1DDJ5_9BACL Radical SAM domain protein Length = 379 Score = 392 bits (1006), Expect = e-107, Method: Compositional matrix adjust. Identities = 184/247 (74%), Positives = 217/247 (87%), Gaps = 3/247 (1%) Query: 11 EITMHSFSPAEIMKMVEVS-GLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKG 69 +ITMHSFSPAEI KM EVS GL L++V+R+++ AGLDSLPGGGAEILDDRTRRKISRLKG Sbjct: 134 DITMHSFSPAEIQKMKEVSEGLSLDEVIRQLNEAGLDSLPGGGAEILDDRTRRKISRLKG 193 Query: 70 SWREWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLA 129 SW +WMDVM++AH+ GMNTTATMV G GE+ EERALHLLR+R+AQDECIAN YDS+GFLA Sbjct: 194 SWTDWMDVMKSAHRHGMNTTATMVYGFGETAEERALHLLRIRDAQDECIANAYDSKGFLA 253 Query: 130 FISWTFQPDNTNL-KLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYG 188 FI W FQPDNTN+ K+++ PEEYLK VA+SR++LDNI NFQSSWVTMGPE+GK SL YG Sbjct: 254 FICWPFQPDNTNMKKVEKSKPEEYLKMVAVSRIMLDNIDNFQSSWVTMGPEIGKQSLSYG 313 Query: 189 CNDFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKID 248 CNDFGSTMIEENVVS+AG THKVNIE I Q+IRE+GKIPAQR+T+Y+ L+++DD + Sbjct: 314 CNDFGSTMIEENVVSAAGTTHKVNIELILQLIRESGKIPAQRDTKYNTLKMYDD-NEHAS 372 Query: 249 NDFVMQN 255 DFVMQN Sbjct: 373 KDFVMQN 379 >A8VX44.1 A8VX44_9BACI Radical SAM domain protein Length = 368 Score = 343 bits (879), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 166/245 (67%), Positives = 199/245 (81%), Gaps = 8/245 (3%) Query: 11 EITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGS 70 +ITMHSFSPAEI KMVEVSG LE+V+ E+ AGLDS+PGGGAEILD+RTRR+ISRLKG+ Sbjct: 132 DITMHSFSPAEIYKMVEVSGKSLEEVLIELKEAGLDSVPGGGAEILDNRTRRRISRLKGT 191 Query: 71 WREWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAF 130 W EW+D M+ K+GM+ TATMVIG GE+ EERALHL R+R+AQD D++ FLAF Sbjct: 192 WEEWIDCMKMVKKVGMHGTATMVIGFGETYEERALHLQRLRDAQD-------DADCFLAF 244 Query: 131 ISWTFQPDNTNLKLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGCN 190 ISWTFQPDNT +K +R T EYLK AI+R+ LDNI NFQSSWVTMG E+GK SL YG N Sbjct: 245 ISWTFQPDNTAMKGERVTSREYLKNTAIARIFLDNIANFQSSWVTMGAEIGKKSLHYGIN 304 Query: 191 DFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKIDND 250 DFGSTM+EENVVSSAGATHKV+ I ++IREAGK PAQR+T+Y+ LRVF + D ++ND Sbjct: 305 DFGSTMMEENVVSSAGATHKVDTNLILRLIREAGKTPAQRDTKYNTLRVFKE-DETVEND 363 Query: 251 FVMQN 255 F+MQN Sbjct: 364 FIMQN 368 >C0Z9A9.1 C0Z9A9_BREBN Putative menaquinone biosynthesis protein Length = 368 Score = 341 bits (875), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 165/245 (67%), Positives = 194/245 (79%), Gaps = 8/245 (3%) Query: 11 EITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGS 70 +ITMHS S AE+MK+ EVS + +E+V++E+ AAGLDSLPG G EILDDRTRRKISRLKGS Sbjct: 132 QITMHSLSVAEVMKIAEVSNVSVEEVLKELKAAGLDSLPGAGGEILDDRTRRKISRLKGS 191 Query: 71 WREWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAF 130 W EW+D + AH+ G+ TATMVIG GE MEER L LLR+R+AQDE + GF AF Sbjct: 192 WTEWIDTQKAAHRAGLPGTATMVIGFGEEMEERVLSLLRIRDAQDE-------TGGFKAF 244 Query: 131 ISWTFQPDNTNLKLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGCN 190 I WTFQPDNTN+K TPEEYLKT+AISRL+LDNI+NFQSSWVTMGP G+LSL YG N Sbjct: 245 IVWTFQPDNTNMKAVNNTPEEYLKTLAISRLMLDNIENFQSSWVTMGPHYGQLSLSYGAN 304 Query: 191 DFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKIDND 250 DFG TM+EENVVS+A T+KVN I ++IR AGKIPAQRNT+YDILRVF D + + D Sbjct: 305 DFGQTMMEENVVSAAACTYKVNTNQILELIRGAGKIPAQRNTQYDILRVFKDGEMA-ERD 363 Query: 251 FVMQN 255 FVMQN Sbjct: 364 FVMQN 368 >Q9K864.1 Q9K864_BACHD BH3143 protein Length = 370 Score = 338 bits (868), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 164/245 (66%), Positives = 195/245 (79%), Gaps = 9/245 (3%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 I MHSFSPAE+ K+ EVS + +E+V+RE+H AGLDS+PGGGAEIL + TR ++SRLK +W Sbjct: 134 IWMHSFSPAEVWKIAEVSSMSVEEVLRELHEAGLDSMPGGGAEILTEETRLRVSRLKITW 193 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 +W++ M+ K+GM+ TATMVIG GES EERALHL RVR+AQDE +E F AFI Sbjct: 194 EQWINAMKATKKVGMHGTATMVIGFGESFEERALHLQRVRDAQDE-------TECFTAFI 246 Query: 132 SWTFQPDNTNL-KLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGCN 190 SW FQP+NT + K + TP +YLK VAISRL LDNI NFQSSWVTMGPEVGKLSLQYGCN Sbjct: 247 SWLFQPENTGMYKTKKLTPRDYLKNVAISRLFLDNIPNFQSSWVTMGPEVGKLSLQYGCN 306 Query: 191 DFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKIDND 250 DFGSTMIEENVVS+AG THKVN I Q+IREAGKIPAQR T Y+I+R F+D +A + D Sbjct: 307 DFGSTMIEENVVSAAGTTHKVNTNKILQLIREAGKIPAQRTTSYEIIRTFEDKEAA-EKD 365 Query: 251 FVMQN 255 FVMQN Sbjct: 366 FVMQN 370 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,451,514,902 Number of extensions: 57924354 Number of successful extensions: 152267 Number of sequences better than 1.0e-05: 518 Number of HSP's gapped: 151387 Number of HSP's successfully gapped: 519 Length of query: 255 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 122 Effective length of database: 1,807,701,208 Effective search space: 220539547376 Effective search space used: 220539547376 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)