BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0028.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB67.1 B3KB67_9BACI Membrane protein-like protein 270 1e-70 B1DDI6.1 B1DDI6_9BACL Membrane protein-like protein 197 2e-48 A7Z8M8.1 A7Z8M8_BACA2 YvqF 108 6e-22 O32199.1 LIAF_BACSU Protein liaF 105 7e-21 B4AG95.1 B4AG95_BACPU Protein LiaF 100 2e-19 >B3KB67.1 B3KB67_9BACI Membrane protein-like protein Length = 369 Score = 270 bits (691), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 153/375 (40%), Positives = 216/375 (57%), Gaps = 25/375 (6%) Query: 1 MKRRFTSQXXXXXXXXXXXAMFLLRQMGYTDFNAGSLFADYWPVILIVMGVQNFLSTNDH 60 M R+ SQ +FLL Q+ + G +F+ +WPV +I G+Q+ + + Sbjct: 3 MSRKGWSQLSNGLLLIGIGVVFLLNQLDIIHVDLGYIFSTFWPVFIIWFGLQSLI----Y 58 Query: 61 GKGSSVFWGFFYLALGVFFLGRNLDWFYVSAGDFFKLLIPVMLIGGGLYVIFKPRNHXXX 120 +G +++ + +G +FLGRNLD + S GD K IP++LIG GL VIFKP++H Sbjct: 59 ARGGALWGSIVPIVIGSYFLGRNLDMIHFSFGDLIKYAIPIILIGYGLQVIFKPKDHTPP 118 Query: 121 XXXXXXXXXXXXXXXXXKSSFDV-EPP--KPLESTLDEQFEQKFGKPE-GKRDWNEYLQK 176 + +D+ EPP +PLES+LDE+FE++FGKP+ + + E + Sbjct: 119 PSPPSPGDFGSHR----RDDYDIPEPPEARPLESSLDEEFERRFGKPDKADKAYEESEFE 174 Query: 177 DDEDEDEDNGHIRSSADSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFND--------- 227 + D G+ + D Sbjct: 175 SRDSHDSSKGYRWQGQHERPRQERPDRSKFRVHVDFDDNGKSYGDGSEYKDEHHHHQHHH 234 Query: 228 ----TDDAKETTHRSAFIGDVHMGKDHFQLKNTNISQFIGDTVLDLTNAQIPYGETKINI 283 + + +T ++S+FIGDVHMGKD+FQLK TNISQFIGDTVLDLT AQIPYGETKINI Sbjct: 235 HHHQSWHSHDTVNKSSFIGDVHMGKDYFQLKPTNISQFIGDTVLDLTKAQIPYGETKINI 294 Query: 284 SAFVGDIKVYVPEDMNLGVKVNSSSFIGDMQVLEQSRSGFMSNVQCKTPYYKEAGKKVRI 343 SAF+GD+KV+VP D ++G+ V ++SFIGDM+VLE+SRSGFMSN ++P+Y+EAGKK+RI Sbjct: 295 SAFIGDVKVFVPNDSDVGIHVTTNSFIGDMKVLEESRSGFMSNAGLESPHYREAGKKIRI 354 Query: 344 NVSAFIGDIKIKTVG 358 VS FIGD+K+ VG Sbjct: 355 TVSVFIGDVKVNRVG 369 >B1DDI6.1 B1DDI6_9BACL Membrane protein-like protein Length = 369 Score = 197 bits (500), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 117/351 (33%), Positives = 176/351 (50%), Gaps = 17/351 (4%) Query: 22 FLLRQMGYTDFNA--GSLFADYWPVILIVMGVQNFLSTND-HGKGSSVFWGFFYLALGVF 78 FLL+Q+G D++ G + ++YWPV+L+ +G+ L+ HG G +W + LG Sbjct: 22 FLLKQLGMIDYDISIGEIISNYWPVVLLWIGLHGLLAGRQGHGSG---WWAGMMIILGFL 78 Query: 79 FLGRNLDWFYVSAGDFFKLLIPVMLIGGGLYVIFKPRNHXXXXXXXXXXXXXXXXXXXXK 138 FLG NL+WF S GD P+++I G + +PR+ K Sbjct: 79 FLGHNLEWFAWSFGDIISYAWPIIIILVGFNFLRRPRHRHQEPPQYEDEWKSYNAYTDYK 138 Query: 139 SSFDVEPP-KPLESTLDEQFEQKFGKPEGKRD-WNEYLQKDDE--DEDEDNGHIRSSAD- 193 PP P + E+K + EG +NE ++ + D E+ H D Sbjct: 139 GEVPSAPPLHPDPTKPGGTDERKEARSEGDSSAFNERNERSEPAGDYREEPLHRMRHHDM 198 Query: 194 ------SXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFNDTDDAKETTHRSAFIGDVHMG 247 F + RS FIGD+H+G Sbjct: 199 RQQFRRQIHEQKRHIREHKQQVREHIREHRQRAKHGSFEYWNHDPHAQSRSGFIGDIHIG 258 Query: 248 KDHFQLKNTNISQFIGDTVLDLTNAQIPYGETKINISAFVGDIKVYVPEDMNLGVKVNSS 307 +D+++LK NIS FIGDT++DLT AQIP+GETKI IS+F+GD+KVYVP D +GV+V SS Sbjct: 259 QDYWELKPLNISHFIGDTIVDLTKAQIPFGETKICISSFIGDVKVYVPNDYEVGVQVVSS 318 Query: 308 SFIGDMQVLEQSRSGFMSNVQCKTPYYKEAGKKVRINVSAFIGDIKIKTVG 358 +F+GD+++L Q G ++ +PYY+E KK+++ VS FIGD+++ VG Sbjct: 319 AFVGDVKILGQKEGGMFKSINIHSPYYEETDKKIKLVVSTFIGDVRVTKVG 369 >A7Z8M8.1 A7Z8M8_BACA2 YvqF Length = 243 Score = 108 bits (271), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 52/126 (41%), Positives = 85/126 (67%), Gaps = 2/126 (1%) Query: 230 DAKETTHRSAFIGDVHMGKDHFQLKNTNISQFIGDTVLDLTNAQIPYGETKINISAFVGD 289 ++++ RS FIG++ M KD F+L + N+S FIGD +DL+ A IP GE+ + IS +G+ Sbjct: 118 ESRQPDLRSFFIGELRMMKDPFELNDLNVSGFIGDVKIDLSKAMIPEGESTMTISGVIGN 177 Query: 290 IKVYVPEDMNLGVKVNSSSFIGDMQVLEQSRSGFMSNVQCKTPYYKEAGKKVRINVSAFI 349 + VYVP D+ V V+SS+FIGDM +L +SG N+ + Y ++ ++++I++S FI Sbjct: 178 VDVYVPPDIE--VSVSSSAFIGDMNLLGSKKSGMSPNLYKVSSEYGDSPRRIKISISLFI 235 Query: 350 GDIKIK 355 GD+ +K Sbjct: 236 GDVDVK 241 >O32199.1 LIAF_BACSU Protein liaF Length = 241 Score = 105 bits (262), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 52/128 (40%), Positives = 81/128 (63%), Gaps = 2/128 (1%) Query: 230 DAKETTHRSAFIGDVHMGKDHFQLKNTNISQFIGDTVLDLTNAQIPYGETKINISAFVGD 289 D K RS FIG++ M K F L + N+S FIGD +DL+ A IP GE+ I IS +G+ Sbjct: 116 DVKHPDMRSFFIGELQMMKQPFDLNDLNVSGFIGDIKIDLSKAMIPEGESTIVISGVIGN 175 Query: 290 IKVYVPEDMNLGVKVNSSSFIGDMQVLEQSRSGFMSNVQCKTPYYKEAGKKVRINVSAFI 349 + +YVP D+ V V+S+ FIGD+ ++ +SG + V + + E+ ++V+++VS FI Sbjct: 176 VDIYVPSDLE--VAVSSAVFIGDINLIGSKKSGLSTKVYAASTDFSESKRRVKVSVSLFI 233 Query: 350 GDIKIKTV 357 GD+ +K V Sbjct: 234 GDVDVKYV 241 >B4AG95.1 B4AG95_BACPU Protein LiaF Length = 241 Score = 100 bits (249), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 50/116 (43%), Positives = 78/116 (67%), Gaps = 2/116 (1%) Query: 240 FIGDVHMGKDHFQLKNTNISQFIGDTVLDLTNAQIPYGETKINISAFVGDIKVYVPEDMN 299 FIG+V + K FQL + IS FIGD +DL+ A I E+ I IS +GD+ ++VP+D+ Sbjct: 126 FIGEVRLMKKPFQLSDLTISGFIGDVKIDLSKAIIAEEESTIVISGLIGDVDIFVPQDIE 185 Query: 300 LGVKVNSSSFIGDMQVLEQSRSGFMSNVQCKTPYYKEAGKKVRINVSAFIGDIKIK 355 V V++S+ IGDM+++++ RSGF S V T Y E+ +KV+I++S IGD+ ++ Sbjct: 186 --VCVSASAAIGDMKIIDEKRSGFGSKVYVMTDNYDESKRKVKISISLLIGDVDVR 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,807,303,770 Number of extensions: 68565264 Number of successful extensions: 217517 Number of sequences better than 1.0e-05: 111 Number of HSP's gapped: 217418 Number of HSP's successfully gapped: 114 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)