BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0027.p (351 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB68.1 B3KB68_9BACI ATP-binding region ATPase domain protein 493 e-137 B1DDI5.1 B1DDI5_9BACL Integral membrane sensor signal transducti... 353 1e-95 Q9KDL3.1 Q9KDL3_BACHD Two-component sensor histidine kinase 185 7e-45 C3BZU6.1 C3BZU6_BACTU Integral membrane sensor signal transducti... 182 6e-44 C2QQI5.1 C2QQI5_BACCE Integral membrane sensor signal transducti... 182 6e-44 >B3KB68.1 B3KB68_9BACI ATP-binding region ATPase domain protein Length = 360 Score = 493 bits (1269), Expect = e-137, Method: Compositional matrix adjust. Identities = 239/336 (71%), Positives = 282/336 (83%) Query: 1 MVKLLSNTKWMLLVYFLLSGGVTAGLMYAGTCLGYIEVQDYRMWLYLCAGIVLFTVIVGY 60 M+ +L N KW++L YFLL G A +Y GYI ++D R+W+Y G++LFT I GY Sbjct: 1 MMGILKNPKWVILFYFLLIGAGVAVFLYTANQSGYIHIEDNRLWVYYTVGVLLFTGIAGY 60 Query: 61 MAGQRIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRVYHEFNMMMDTVENKMQLLQR 120 +AGQRIQR +DLL LNMLQVAKGNL+VR+P +D SF RVY EFN M + +E KM+LLQR Sbjct: 61 IAGQRIQRPLDLLHLNMLQVAKGNLNVRLPAMEDGSFARVYDEFNGMTEALEKKMKLLQR 120 Query: 121 LGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLERNAEHGKTV 180 LGEQ+VIEKE AAE+AVLEERRRMARDLHDTVSQQLFAIHMSASSLP VL+RN E G V Sbjct: 121 LGEQDVIEKEIAAEAAVLEERRRMARDLHDTVSQQLFAIHMSASSLPAVLKRNPEQGDKV 180 Query: 181 MDQLITMSQMAQKQMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKGVKELELQGE 240 + QLI MS +AQKQMRALIAQLRPVELEGR L EALE+WFPDYCRQNGLKG+K++EL G Sbjct: 181 LGQLIEMSHVAQKQMRALIAQLRPVELEGRTLEEALERWFPDYCRQNGLKGMKDVELYGG 240 Query: 241 LSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGFEHVQHKQGS 300 +S+AIEHQLFLIIQE++ANIVKHAGA +VS+SLRE ++V+LSISDDG GF+ V KQGS Sbjct: 241 VSDAIEHQLFLIIQEAVANIVKHAGAGLVSMSLREDQKRVILSISDDGVGFDEVAGKQGS 300 Query: 301 YGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIPKF 336 YGL+TMRERAEKLGGQV+IIS+KGAGTT+RV+IP F Sbjct: 301 YGLSTMRERAEKLGGQVDIISKKGAGTTVRVNIPLF 336 >B1DDI5.1 B1DDI5_9BACL Integral membrane sensor signal transduction histidine kinase Length = 348 Score = 353 bits (906), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 187/344 (54%), Positives = 242/344 (70%), Gaps = 17/344 (4%) Query: 1 MVKLLSNTKWMLLVYFLLSGGVTAGLM----YAGTCLG-YIEVQ----DYRMWLYLCAGI 51 +V+L KW LL F+ S VTA L+ +AG G Y++ + ++L + AG Sbjct: 2 IVRLFRGGKWQLLTMFVASS-VTALLLGIAVWAGLADGDYVQTEVRYGAVSVFLLVSAGF 60 Query: 52 VLFTVIVGYMAGQRIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRVYHEFNMMMDTV 111 GY GQ IQRRID L L + Q GN VR+ ES D SF+ +Y EFN + + + Sbjct: 61 -------GYWTGQHIQRRIDTLQLALKQAVNGNFQVRITESGDSSFSELYREFNDLAELM 113 Query: 112 ENKMQLLQRLGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLE 171 E +M+ LQ GE++V+ + + E+AV EER+R+ARDLHDTVSQQLFA+HM ASSLPK+ E Sbjct: 114 EERMKRLQMTGEEQVMSQAASNEAAVQEERKRLARDLHDTVSQQLFAMHMCASSLPKLQE 173 Query: 172 RNAEHGKTVMDQLITMSQMAQKQMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKG 231 + E +V+DQLI MS +AQKQMR IAQLRP+ELEGR L EAL+KWFPDYCRQNGL+G Sbjct: 174 IDRERAASVLDQLIQMSTLAQKQMRIFIAQLRPMELEGRTLLEALDKWFPDYCRQNGLQG 233 Query: 232 VKELELQGELSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGF 291 V + L L+EA EHQLFLI+QE+MAN+VKH+GA+ SLSL E RQVVLS+ DDG GF Sbjct: 234 VFDWRLTEPLTEAKEHQLFLIVQEAMANVVKHSGAQGCSLSLTENERQVVLSLQDDGAGF 293 Query: 292 EHVQHKQGSYGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIPK 335 Q ++GSYGL+TM+ERA++LGG EIIS+ G+GT ++V IPK Sbjct: 294 RADQVRRGSYGLSTMQERAQQLGGLAEIISKPGSGTRVKVTIPK 337 >Q9KDL3.1 Q9KDL3_BACHD Two-component sensor histidine kinase Length = 351 Score = 185 bits (469), Expect = 7e-45, Method: Compositional matrix adjust. Identities = 108/294 (36%), Positives = 168/294 (57%), Gaps = 4/294 (1%) Query: 45 LYLCAGIVLFTVIVGYMAGQ----RIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRV 100 L + I+L + +G +AG I+R++D + +L +G S ++ + + Sbjct: 52 LPILVPILLSMIAIGLIAGMMQSLPIKRKLDQILHGVLLYERGTFSHQIESEGEDELAEL 111 Query: 101 YHEFNMMMDTVENKMQLLQRLGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIH 160 N M + VE ++ LQRL + +E ++AV EER+R+ARDLHD VSQQLFAI Sbjct: 112 TDRLNRMAERVEEQVASLQRLSSERAKMQETVKKAAVTEERQRLARDLHDAVSQQLFAIS 171 Query: 161 MSASSLPKVLERNAEHGKTVMDQLITMSQMAQKQMRALIAQLRPVELEGRNLFEALEKWF 220 M +++ + L E + MD + M+ AQ +MRAL+ LRP ELEG++L + +E Sbjct: 172 MMTAAIKQNLHEATEEVQQQMDVVEKMANTAQAEMRALLLHLRPAELEGKSLQQGVEYLL 231 Query: 221 PDYCRQNGLKGVKELELQGELSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQV 280 + ++ G K K LE EL + +E QLF ++QE+++NI++HA A V + + RQV Sbjct: 232 HELEQKQGFKITKRLEPMDELPKGVEDQLFRMLQEAISNILRHAQAHHVEVRFWQTERQV 291 Query: 281 VLSISDDGQGFEHVQHKQGSYGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIP 334 L + DDG GF+ + GSYGL TM ER ++GG ++I+S G GT + IP Sbjct: 292 RLKLIDDGVGFDVQKETHGSYGLQTMHERINEIGGVLDIVSAPGKGTQLEAKIP 345 >C3BZU6.1 C3BZU6_BACTU Integral membrane sensor signal transduction histidine kinase Length = 351 Score = 182 bits (461), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 118/350 (33%), Positives = 193/350 (55%), Gaps = 19/350 (5%) Query: 7 NTKWMLLVYFLLSGGVTAGLMYAGTCLGYIEVQDYRM-W----------LYLCAGIVLFT 55 N WM + Y +LS V+ L+ + E Q Y + W L++ +L Sbjct: 6 NISWMYIRYSMLSS-VSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIG 64 Query: 56 VIVGYMAGQRIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRVYHEFNMMMDTVENKM 115 IVGY G I++RI L+ + +V +GN + + D F V + ++ +E + Sbjct: 65 GIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQA 124 Query: 116 QLLQRLGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLERNAE 175 L Q++ + E+ + A+ +ER R+AR+LHD+VSQQLFA+ M S++ E+ AE Sbjct: 125 GLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAIN---EQVAE 181 Query: 176 HGKTVMDQLITMSQM---AQKQMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKGV 232 +T QL + M AQ +MRAL+ LRPV+LEG+ L E +E+ + R+ +K + Sbjct: 182 FPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK-I 240 Query: 233 KELELQGELSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGFE 292 + L EL + +E LF I+QE+++N ++HA A+ + LR++ + +L I DDG GFE Sbjct: 241 EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFE 300 Query: 293 HVQHKQGSYGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIPKFLQGGPE 342 +K GSYGL +M+ER ++GG ++++S GT I V +P ++ G E Sbjct: 301 VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPNKGTQIEVKVPIMIERGGE 350 >C2QQI5.1 C2QQI5_BACCE Integral membrane sensor signal transduction histidine kinase Length = 351 Score = 182 bits (461), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 118/350 (33%), Positives = 193/350 (55%), Gaps = 19/350 (5%) Query: 7 NTKWMLLVYFLLSGGVTAGLMYAGTCLGYIEVQDYRM-W----------LYLCAGIVLFT 55 N WM + Y +LS V+ L+ + E Q Y + W L++ +L Sbjct: 6 NISWMYIRYSMLSS-VSIALICTIVYVWKSEQQVYDLLWKESIASVPIGLFIMTTSLLIG 64 Query: 56 VIVGYMAGQRIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRVYHEFNMMMDTVENKM 115 IVGY G I++RI L+ + +V +GN + + D F V + ++ +E + Sbjct: 65 GIVGYAIGYYIEQRIQGLNTFLFEVERGNFPSDVSFTADDEFHEVERKVIVLARRLEEQA 124 Query: 116 QLLQRLGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLERNAE 175 L Q++ + E+ + A+ +ER R+AR+LHD+VSQQLFA+ M S++ E+ AE Sbjct: 125 GLFQKVTNERAHWNEEMRQEAISQERHRLARELHDSVSQQLFAMSMMMSAIN---EQVAE 181 Query: 176 HGKTVMDQLITMSQM---AQKQMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKGV 232 +T QL + M AQ +MRAL+ LRPV+LEG+ L E +E+ + R+ +K + Sbjct: 182 FPETTKKQLQLVENMVVNAQSEMRALLLHLRPVQLEGKKLTEGIEELLTELSRKQHMK-I 240 Query: 233 KELELQGELSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGFE 292 + L EL + +E LF I+QE+++N ++HA A+ + LR++ + +L I DDG GFE Sbjct: 241 EWLIEPIELKKGVEDHLFRIVQEALSNTLRHAKAKKTEVRLRKIDQYAILKIIDDGVGFE 300 Query: 293 HVQHKQGSYGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIPKFLQGGPE 342 +K GSYGL +M+ER ++GG ++++S GT I V +P ++ G E Sbjct: 301 VGVNKAGSYGLRSMQERVHEIGGTLKVLSFPNKGTQIEVKVPIMIERGGE 350 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,870,759,746 Number of extensions: 72367416 Number of successful extensions: 279383 Number of sequences better than 1.0e-05: 3680 Number of HSP's gapped: 274174 Number of HSP's successfully gapped: 3682 Length of query: 351 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 215 Effective length of database: 1,779,438,163 Effective search space: 382579205045 Effective search space used: 382579205045 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)