BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0021.p (318 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KB73.1 B3KB73_9BACI Hydroxymethylbutenyl pyrophosphate reducta... 563 e-159 B1DDH9.1 B1DDH9_9BACL Hydroxymethylbutenyl pyrophosphate reducta... 523 e-146 Q9KD37.1 ISPH_BACHD 4-hydroxy-3-methylbut-2-enyl diphosphate red... 497 e-139 C0ZL73.1 ISPH_BREBN 4-hydroxy-3-methylbut-2-enyl diphosphate red... 493 e-137 Q5WHC6.1 ISPH_BACSK 4-hydroxy-3-methylbut-2-enyl diphosphate red... 481 e-134 >B3KB73.1 B3KB73_9BACI Hydroxymethylbutenyl pyrophosphate reductase EC=1.17.1.2 Length = 326 Score = 563 bits (1451), Expect = e-159, Method: Compositional matrix adjust. Identities = 268/316 (84%), Positives = 295/316 (93%) Query: 1 MEVIKISPRGYCYGVVDAMVMARQAAQNLDLPRPIYILGMIVHNSHVTNSFEDDGIITLD 60 +EV+KISPRGYCYGVVDAMV+ARQAAQNLDLPRPIYILGMIVHNSHVT SFED+GIITLD Sbjct: 11 IEVLKISPRGYCYGVVDAMVLARQAAQNLDLPRPIYILGMIVHNSHVTQSFEDEGIITLD 70 Query: 61 GHNRLDILDKVDSGTVIFTAHGVSPEVRRLARAKGLTTVDATCPDVTKTHDLIKEKVAEG 120 G NR++IL +V+SGTVIFTAHGVSPEVR+LAR KGLTT+DATCPDVTKTHDLI++KVA+G Sbjct: 71 GPNRMEILSQVESGTVIFTAHGVSPEVRKLARDKGLTTLDATCPDVTKTHDLIRDKVADG 130 Query: 121 CEIIYIGKKGHPEPEGAVGIAPEHVHLIEKEEEIAGLSIPSSRIVITNQTTMSQWDIKHI 180 EIIYIGKKGHPEPEGA+GIAPEHVHLIEKEEEI L + +SRI+ITNQTTMSQWDIKHI Sbjct: 131 YEIIYIGKKGHPEPEGAIGIAPEHVHLIEKEEEIEQLQVKTSRIIITNQTTMSQWDIKHI 190 Query: 181 MKKLLEAFPGAEVHNEICMATQVRQEAVAEQAGQCDLVIVVGDPRSNNSNRLAQVSEEIA 240 MK LL+ FPGAE+HNEIC+ATQVRQEAVAEQAGQ DLVIVVGDPRSNNSNRLAQVSEEIA Sbjct: 191 MKSLLDKFPGAEIHNEICLATQVRQEAVAEQAGQADLVIVVGDPRSNNSNRLAQVSEEIA 250 Query: 241 GVPAHRIADLSELKTEWLQGITKVGVTSGASTPTPITKEVISYLEQYDPALPETWEIKRT 300 GVPAHRI D++ELK EWL+G+ KV VTSGASTPTPITKEVI+YLEQY+ PETWEIKRT Sbjct: 251 GVPAHRIGDVTELKREWLEGVGKVAVTSGASTPTPITKEVIAYLEQYEHDQPETWEIKRT 310 Query: 301 VNMSKLLPPVKNKSAS 316 VNMSKLLPPV+ KS S Sbjct: 311 VNMSKLLPPVRVKSKS 326 >B1DDH9.1 B1DDH9_9BACL Hydroxymethylbutenyl pyrophosphate reductase EC=1.17.1.2 Length = 321 Score = 523 bits (1346), Expect = e-146, Method: Compositional matrix adjust. Identities = 244/318 (76%), Positives = 285/318 (89%) Query: 1 MEVIKISPRGYCYGVVDAMVMARQAAQNLDLPRPIYILGMIVHNSHVTNSFEDDGIITLD 60 ME++KISPRGYCYGVVDAMV+A Q A+NLDLPRPIYILGMIVHN+HVT +FE +GIITLD Sbjct: 1 MEIVKISPRGYCYGVVDAMVLALQTAKNLDLPRPIYILGMIVHNAHVTEAFEQEGIITLD 60 Query: 61 GHNRLDILDKVDSGTVIFTAHGVSPEVRRLARAKGLTTVDATCPDVTKTHDLIKEKVAEG 120 G NRL+IL++V+SGTVIFTAHGVSPEVR AR KGLT VDATCPDVTKTHDLI+EKVAE Sbjct: 61 GENRLEILEQVESGTVIFTAHGVSPEVRNRAREKGLTVVDATCPDVTKTHDLIREKVAED 120 Query: 121 CEIIYIGKKGHPEPEGAVGIAPEHVHLIEKEEEIAGLSIPSSRIVITNQTTMSQWDIKHI 180 IIYIGKKGHPEPEGA+G+AP+ VHLIE+ E+I+ L++P RI+ITNQTTMSQWDI+HI Sbjct: 121 YHIIYIGKKGHPEPEGAIGVAPDRVHLIERVEDISKLNVPEGRIIITNQTTMSQWDIRHI 180 Query: 181 MKKLLEAFPGAEVHNEICMATQVRQEAVAEQAGQCDLVIVVGDPRSNNSNRLAQVSEEIA 240 + KLLE +P AE+HNEIC+ATQVRQEAVAEQAGQ L IVVGDPRSNNSNRLAQVS+EIA Sbjct: 181 ISKLLETYPRAEIHNEICLATQVRQEAVAEQAGQAQLCIVVGDPRSNNSNRLAQVSQEIA 240 Query: 241 GVPAHRIADLSELKTEWLQGITKVGVTSGASTPTPITKEVISYLEQYDPALPETWEIKRT 300 GVPA+R++D+SE++ EWL+G+ +V VTSGASTPTPITKEVI+YLEQYD P TWEIKRT Sbjct: 241 GVPAYRVSDVSEIEQEWLKGVDRVAVTSGASTPTPITKEVIAYLEQYDHNDPSTWEIKRT 300 Query: 301 VNMSKLLPPVKNKSASTT 318 VN +KLLP ++ KSAS+T Sbjct: 301 VNKNKLLPTIRTKSASST 318 >Q9KD37.1 ISPH_BACHD 4-hydroxy-3-methylbut-2-enyl diphosphate reductase EC=1.17.1.2 Length = 314 Score = 497 bits (1280), Expect = e-139, Method: Compositional matrix adjust. Identities = 228/313 (72%), Positives = 272/313 (86%) Query: 1 MEVIKISPRGYCYGVVDAMVMARQAAQNLDLPRPIYILGMIVHNSHVTNSFEDDGIITLD 60 M V+KISPRGYCYGVVDAMV+ARQAAQNLDLPRPIYILGMIVHN HVT++FE++GII+LD Sbjct: 1 MNVVKISPRGYCYGVVDAMVLARQAAQNLDLPRPIYILGMIVHNKHVTDAFEEEGIISLD 60 Query: 61 GHNRLDILDKVDSGTVIFTAHGVSPEVRRLARAKGLTTVDATCPDVTKTHDLIKEKVAEG 120 G NRLDIL +VD GTVIFTAHGVSP+VR+LA+ KGLT VDATCPDVT+THDLI+EK EG Sbjct: 61 GPNRLDILKQVDKGTVIFTAHGVSPQVRKLAKEKGLTVVDATCPDVTRTHDLIREKSQEG 120 Query: 121 CEIIYIGKKGHPEPEGAVGIAPEHVHLIEKEEEIAGLSIPSSRIVITNQTTMSQWDIKHI 180 + IY+GKKGHPEPEGA+G+APE VHL+E E++ L I + +I+ITNQTTMSQWD+ I Sbjct: 121 YKFIYVGKKGHPEPEGAIGVAPEFVHLVENVEDVESLDISADKIIITNQTTMSQWDVSEI 180 Query: 181 MKKLLEAFPGAEVHNEICMATQVRQEAVAEQAGQCDLVIVVGDPRSNNSNRLAQVSEEIA 240 MKK +E +P AEVHNEIC+ATQVRQEAVAEQA +CDLVIVVGDP+SNNSNRLAQVSE+IA Sbjct: 181 MKKAMEKYPQAEVHNEICLATQVRQEAVAEQARECDLVIVVGDPKSNNSNRLAQVSEQIA 240 Query: 241 GVPAHRIADLSELKTEWLQGITKVGVTSGASTPTPITKEVISYLEQYDPALPETWEIKRT 300 G A+RI D++EL+ EW G+ VGVT+GASTPTPITKEVI+++E+YDP+ PETW +R Sbjct: 241 GTKAYRIGDVTELQQEWFDGVETVGVTAGASTPTPITKEVIAFIEKYDPSKPETWTPERK 300 Query: 301 VNMSKLLPPVKNK 313 V + K+LP VK K Sbjct: 301 VTLQKILPKVKIK 313 >C0ZL73.1 ISPH_BREBN 4-hydroxy-3-methylbut-2-enyl diphosphate reductase EC=1.17.1.2 Length = 311 Score = 493 bits (1269), Expect = e-137, Method: Compositional matrix adjust. Identities = 229/311 (73%), Positives = 268/311 (86%) Query: 1 MEVIKISPRGYCYGVVDAMVMARQAAQNLDLPRPIYILGMIVHNSHVTNSFEDDGIITLD 60 MEVIKISPRGYCYGVVDAMV+A + AQN DLPRPI+ILGMIVHN+HV +FE GI TLD Sbjct: 1 MEVIKISPRGYCYGVVDAMVLALRTAQNFDLPRPIHILGMIVHNAHVVEAFEKQGIKTLD 60 Query: 61 GHNRLDILDKVDSGTVIFTAHGVSPEVRRLARAKGLTTVDATCPDVTKTHDLIKEKVAEG 120 G +RL +LD+V GT+IFTAHGVSPEVR+ AR KGLT VDATCPDVTKTHDLI+EKVAEG Sbjct: 61 GEDRLALLDQVQEGTIIFTAHGVSPEVRKKARDKGLTVVDATCPDVTKTHDLIREKVAEG 120 Query: 121 CEIIYIGKKGHPEPEGAVGIAPEHVHLIEKEEEIAGLSIPSSRIVITNQTTMSQWDIKHI 180 ++YIGKKGHPEPEGA+GIAP+HVHL++K EE+ L +P+ ++++TNQTTMSQWD+KH+ Sbjct: 121 YHVLYIGKKGHPEPEGAMGIAPDHVHLVQKLEELEALDLPTDKLIVTNQTTMSQWDVKHL 180 Query: 181 MKKLLEAFPGAEVHNEICMATQVRQEAVAEQAGQCDLVIVVGDPRSNNSNRLAQVSEEIA 240 M +L+ FPG EVHNEIC+ATQVRQEAVAEQ G+ DL IVVGDPRSNNSNRLAQVSEEIA Sbjct: 181 MDAILKRFPGVEVHNEICLATQVRQEAVAEQVGEADLCIVVGDPRSNNSNRLAQVSEEIA 240 Query: 241 GVPAHRIADLSELKTEWLQGITKVGVTSGASTPTPITKEVISYLEQYDPALPETWEIKRT 300 VP++RIADL EL EWL+G V VTSGASTPTP+TKEVI++LEQ+D P TWE KRT Sbjct: 241 DVPSYRIADLFELDIEWLRGKKNVAVTSGASTPTPLTKEVITFLEQFDEFNPATWEKKRT 300 Query: 301 VNMSKLLPPVK 311 VNM+K+LP VK Sbjct: 301 VNMAKILPTVK 311 >Q5WHC6.1 ISPH_BACSK 4-hydroxy-3-methylbut-2-enyl diphosphate reductase EC=1.17.1.2 Length = 314 Score = 481 bits (1239), Expect = e-134, Method: Compositional matrix adjust. Identities = 224/313 (71%), Positives = 267/313 (85%) Query: 1 MEVIKISPRGYCYGVVDAMVMARQAAQNLDLPRPIYILGMIVHNSHVTNSFEDDGIITLD 60 M+V+KISPRGYCYGVVDAMVMARQAAQNLDLPRP+YILG IVHN HVT++F++DGII+LD Sbjct: 1 MDVLKISPRGYCYGVVDAMVMARQAAQNLDLPRPLYILGQIVHNQHVTDAFKEDGIISLD 60 Query: 61 GHNRLDILDKVDSGTVIFTAHGVSPEVRRLARAKGLTTVDATCPDVTKTHDLIKEKVAEG 120 G NRL+IL V GTVIFTAHGVSPEVRRLA+ KGLT +DATCPDVT+THDLI+EK EG Sbjct: 61 GPNRLEILKNVTHGTVIFTAHGVSPEVRRLAKEKGLTCIDATCPDVTRTHDLIREKAEEG 120 Query: 121 CEIIYIGKKGHPEPEGAVGIAPEHVHLIEKEEEIAGLSIPSSRIVITNQTTMSQWDIKHI 180 + IYIGK+GHPEPEGA+G+AP+ VHL+E E++ L++ I+ITNQTTMSQWD+ I Sbjct: 121 YQFIYIGKQGHPEPEGAMGVAPDSVHLVETMEDLEQLTLDDRDIIITNQTTMSQWDVADI 180 Query: 181 MKKLLEAFPGAEVHNEICMATQVRQEAVAEQAGQCDLVIVVGDPRSNNSNRLAQVSEEIA 240 MK+ +E +P AEVHNEIC+ATQVRQEAVAEQA QCD+VIVVGDP+SNNSNRLAQVSE+IA Sbjct: 181 MKRAMELYPNAEVHNEICLATQVRQEAVAEQASQCDIVIVVGDPKSNNSNRLAQVSEQIA 240 Query: 241 GVPAHRIADLSELKTEWLQGITKVGVTSGASTPTPITKEVISYLEQYDPALPETWEIKRT 300 G PA+RI DLSELK EW++ KVGVTSGASTPTPITKEVI+++E YDP TW+ + Sbjct: 241 GTPAYRIGDLSELKLEWIKEAKKVGVTSGASTPTPITKEVITFIENYDPNDESTWDTAKK 300 Query: 301 VNMSKLLPPVKNK 313 V + K+LP V+ K Sbjct: 301 VPIEKILPKVRVK 313 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,902,778,417 Number of extensions: 78864991 Number of successful extensions: 214835 Number of sequences better than 1.0e-05: 1330 Number of HSP's gapped: 210489 Number of HSP's successfully gapped: 1340 Length of query: 318 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 183 Effective length of database: 1,788,859,178 Effective search space: 327361229574 Effective search space used: 327361229574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)