BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0003.p (377 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1KY81.1 B1KY81_CLOBM Glycerol dehydrogenase EC=1.1.1.6 577 e-163 A7FSM3.1 A7FSM3_CLOB1 Glycerol dehydrogenase EC=1.1.1.6 577 e-163 A5I0G8.1 A5I0G8_CLOBH Glycerol dehydrogenase EC=1.1.1.6 577 e-163 A7GC18.1 A7GC18_CLOBL Glycerol dehydrogenase EC=1.1.1.6 577 e-163 B1IHW0.1 B1IHW0_CLOBK Glycerol dehydrogenase EC=1.1.1.6 577 e-163 >B1KY81.1 B1KY81_CLOBM Glycerol dehydrogenase EC=1.1.1.6 Length = 376 Score = 577 bits (1488), Expect = e-163, Method: Compositional matrix adjust. Identities = 277/376 (73%), Positives = 315/376 (83%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGEDELLNLGYFVK+FG+SALLIAH DD++RVK KLD+T+ KFGIT + Sbjct: 1 MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 +F GECSR+EVARLQ +A+ + CACTIGLGGGKAIDTAKCVA+G++LIIVPTIAATDA Sbjct: 61 ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYTP+GAFDDYAYF QSP+V++IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SFSNVNAGLPCGVREG+ AKGT AL LA+ CY+TLLEDGVKAK+A D N+VT AL Sbjct: 181 SNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIETNI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLENA Sbjct: 241 ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 EI EV+ FC +GLPVCL DIGV SIT EEL+EVA+K+CI EESIHSMPF VTPEAVAA Sbjct: 301 EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA 360 Query: 361 AITVADQLGHAFKKGK 376 AI AD LG +K K Sbjct: 361 AIITADTLGKKYKSNK 376 >A7FSM3.1 A7FSM3_CLOB1 Glycerol dehydrogenase EC=1.1.1.6 Length = 376 Score = 577 bits (1488), Expect = e-163, Method: Compositional matrix adjust. Identities = 277/376 (73%), Positives = 315/376 (83%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGEDELLNLGYFVK+FG+SALLIAH DD++RVK KLD+T+ KFGIT + Sbjct: 1 MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 +F GECSR+EVARLQ +A+ + CACTIGLGGGKAIDTAKCVA+G++LIIVPTIAATDA Sbjct: 61 ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYTP+GAFDDYAYF QSP+V++IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SFSNVNAGLPCGVREG+ AKGT AL LA+ CY+TLLEDGVKAK+A D N+VT AL Sbjct: 181 SNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIETNI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLENA Sbjct: 241 ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 EI EV+ FC +GLPVCL DIGV SIT EEL+EVA+K+CI EESIHSMPF VTPEAVAA Sbjct: 301 EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA 360 Query: 361 AITVADQLGHAFKKGK 376 AI AD LG +K K Sbjct: 361 AIITADTLGKKYKSNK 376 >A5I0G8.1 A5I0G8_CLOBH Glycerol dehydrogenase EC=1.1.1.6 Length = 376 Score = 577 bits (1488), Expect = e-163, Method: Compositional matrix adjust. Identities = 277/376 (73%), Positives = 315/376 (83%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGEDELLNLGYFVK+FG+SALLIAH DD++RVK KLD+T+ KFGIT + Sbjct: 1 MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 +F GECSR+EVARLQ +A+ + CACTIGLGGGKAIDTAKCVA+G++LIIVPTIAATDA Sbjct: 61 ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYTP+GAFDDYAYF QSP+V++IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SFSNVNAGLPCGVREG+ AKGT AL LA+ CY+TLLEDGVKAK+A D N+VT AL Sbjct: 181 SNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIETNI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLENA Sbjct: 241 ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 EI EV+ FC +GLPVCL DIGV SIT EEL+EVA+K+CI EESIHSMPF VTPEAVAA Sbjct: 301 EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA 360 Query: 361 AITVADQLGHAFKKGK 376 AI AD LG +K K Sbjct: 361 AIITADTLGKKYKSNK 376 >A7GC18.1 A7GC18_CLOBL Glycerol dehydrogenase EC=1.1.1.6 Length = 376 Score = 577 bits (1488), Expect = e-163, Method: Compositional matrix adjust. Identities = 276/376 (73%), Positives = 315/376 (83%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGEDELLNLGYFVK+FG+SALLIAH DD++RVK KLD+T+ KFGIT + Sbjct: 1 MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 +F GECSR+EVARLQ +A+ + CACTIGLGGGKAIDTAKCVA+G++LIIVPTIAATDA Sbjct: 61 ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYTP+G+FDDYAYF QSP+V++IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTPDGSFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SFSNVNAGLPCGVREG+ AKGT AL LA+ CY+TLLEDGVKAK+A D N+VT AL Sbjct: 181 SNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIETNI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLENA Sbjct: 241 ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 EI EV+ FC +GLPVCL DIGV SIT EEL+EVA+K+CI EESIHSMPF VTPEAVAA Sbjct: 301 EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA 360 Query: 361 AITVADQLGHAFKKGK 376 AI AD LG +K K Sbjct: 361 AIITADTLGKKYKSNK 376 >B1IHW0.1 B1IHW0_CLOBK Glycerol dehydrogenase EC=1.1.1.6 Length = 376 Score = 577 bits (1487), Expect = e-163, Method: Compositional matrix adjust. Identities = 276/376 (73%), Positives = 315/376 (83%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 MRK FI PTKY+QGEDELLNLGYFVK+FG+SALLIAH DD++RVK KLD+T+ KFGIT + Sbjct: 1 MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAEGDALIIVPTIAATDA 120 +F GECSR+EVARLQ +A+ + CACTIGLGGGKAIDTAKCVA+G++LIIVPTIAATDA Sbjct: 61 ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA 120 Query: 121 PTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEARAT 180 PTSHSAVLYTP+G+FDDYAYF QSP+V++IDTT+IA APTRFLVSGMGDALSTYFEARAT Sbjct: 121 PTSHSAVLYTPDGSFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT 180 Query: 181 ARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTPAL 240 + SFSNVNAGLPCGVREG+ AKGT AL LA+ CY+TLLEDGVKAK+A D N+VT AL Sbjct: 181 SNSFSNVNAGLPCGVREGLCAPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL 240 Query: 241 ENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENADS 300 ENIIETNI HAIH+GLT+LEG+H Y+HGEKVAF T+AQLVLENA Sbjct: 241 ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK 300 Query: 301 REIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAVAA 360 EI EV+ FC +GLPVCL DIGV SIT EEL+EVA+K+CI EESIHSMPF VTPEAVAA Sbjct: 301 EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA 360 Query: 361 AITVADQLGHAFKKGK 376 AI AD LG +K K Sbjct: 361 AIITADTLGKEYKSNK 376 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,024,401,639 Number of extensions: 77128416 Number of successful extensions: 197449 Number of sequences better than 1.0e-05: 694 Number of HSP's gapped: 195666 Number of HSP's successfully gapped: 709 Length of query: 377 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 240 Effective length of database: 1,770,017,148 Effective search space: 424804115520 Effective search space used: 424804115520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)