BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0538.0019.p (307 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q1G8G2.1 Q1G8G2_LACDA Transcriptional regulator (LysR family) 154 8e-36 B4AJ13.1 B4AJ13_BACPU HTH-type transcriptional regulator AlsR (A... 138 8e-31 Q88SC9.1 Q88SC9_LACPL Transcription regulator 137 1e-30 C2FPU6.1 C2FPU6_LACPL Transcription regulator 137 1e-30 Q181P0.1 Q181P0_CLOD6 LysR-family transcriptional regulator 136 2e-30 >Q1G8G2.1 Q1G8G2_LACDA Transcriptional regulator (LysR family) Length = 295 Score = 154 bits (390), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 86/291 (29%), Positives = 159/291 (54%), Gaps = 2/291 (0%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 M ++ L FV+L ETLNFSKTA LN++Q ++S I S+E +LG+ LF R+++ V+LT A Sbjct: 1 MYLEHLTTFVNLVETLNFSKTAVNLNISQSSISQAIASIEKQLGVTLFYRSRKAVSLTPA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 G+ FY+ ++ L +++++ +++ + L IGY+ T +E V+P+++R F +++PQ Sbjct: 61 GQEFYDRVKPWLNEYNKAVQHVQQVQNAQKMNLTIGYSGTPYEHTVVPELVRAFCQEHPQ 120 Query: 135 IQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPFAN 194 I + L+ K L + D+IFT D + + ++ +L G Y ++P Sbjct: 121 INVFLENYAHQELKGHLTHGNCDVIFTMPDIIAGMDNLAYHSLLIGYYCLIVPSEVKLG- 179 Query: 195 ESELDIEQLNHQSIILFDEHQSPPTLKKMNRKIIENCPDSIYTYGDTINTVHTMIKSDLG 254 + +E+L+ +I D PPT ++ ++I N Y + I T M+K+ LG Sbjct: 180 PPPISLEELSKNRLIFLDHRWCPPTQDQLQKEICRNNHSLDLAYVNNIATARAMVKARLG 239 Query: 255 VSVLPDFVIG-EDSEIASVPLSYTEEVVYGLACLRSEVRLEVKRWISTIKE 304 + +FV D ++ +PLS YG+A L+ ++++S +++ Sbjct: 240 WGIWANFVSDPNDLDLKKLPLSTKIVPDYGVAILKQGSPQAARQFVSWLEK 290 >B4AJ13.1 B4AJ13_BACPU HTH-type transcriptional regulator AlsR (Als operon regulatoryprotein) Length = 298 Score = 138 bits (347), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 80/254 (31%), Positives = 140/254 (55%), Gaps = 20/254 (7%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 M ++ L+ FV++AE L+F + A +LN+TQP +S IK E ELG LF R+KR V LT A Sbjct: 1 MELRHLQYFVTVAEELHFGRAAARLNMTQPPLSQQIKQFEEELGFPLFHRSKRVVELTAA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F ++ G+L +LD+++ A+ + ++IG+ T +LP ++R F+E YP Sbjct: 61 GKVFLHEVRGVLHQLDKAVDHARHTARGELGRIIIGFVGTA-TYDILPPVVREFRELYPS 119 Query: 135 IQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPFAN 194 + I+L++ ++ ++ E LLN + DI F + +++ + Q + +PKNHP A Sbjct: 120 VSIELEQLSVPQQLEALLNGEIDIGFLHPTSPHEELVSRL--MKQSECIFAIPKNHPLAK 177 Query: 195 ESELDIEQLNHQSIILFDEHQSPPTLKKMNRKIIENCPDSIYTYGDTINTVH-------- 246 + + IE + H+ II + +S P+L + + E YG + N V Sbjct: 178 KEAVTIEDIRHEPIISLSQ-ESWPSLYQHFVLLCEK-------YGFSPNIVQEAAEYQMV 229 Query: 247 -TMIKSDLGVSVLP 259 ++ + +G++V+P Sbjct: 230 IGLVTAGIGIAVIP 243 >Q88SC9.1 Q88SC9_LACPL Transcription regulator Length = 301 Score = 137 bits (346), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 86/269 (31%), Positives = 147/269 (54%), Gaps = 15/269 (5%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 MN+ L+ FV+L+ T N+++TA+ L++TQP+++ I SLE EL I LF + R+V LT A Sbjct: 1 MNLDHLRYFVALSRTENYTQTAKLLHITQPSLTKAIHSLEAELNIDLFQKAGRSVTLTPA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F D+E L LD+S+ KR ++ ++ + I T+ K LPD+ +RF + P Sbjct: 61 GKVFASDVESALLLLDKSVTNIKRFDQQ-KAPIRIAALRTL-SIKWLPDIAQRFLQLSPH 118 Query: 135 IQIQLKKSNLTREKEDLL----NQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNH 190 +Q + + T D+L N K+D+ F S+ + DI+ F+PV + + VC+ P H Sbjct: 119 AAVQFQFNTDTGLSPDILNGLRNNKYDVAFCSKMDHFSDIE--FFPVAEQTMVCITPTTH 176 Query: 191 PFANESELDIEQLNHQSIILFDEHQSPPTLKKMNRKIIENC---PDSIYTYGDTINTVHT 247 P AN S +++ + I F + L + +K+ +C P S Y + + Sbjct: 177 PLANRSSINLAETLAYPQITFSQRSG---LHPIMQKLFNDCGGQPISAYAVEED-QAIAG 232 Query: 248 MIKSDLGVSVLPDFVIGEDSEIASVPLSY 276 ++ S G++V+P+ I + + +PLS+ Sbjct: 233 LVASGFGIAVVPNMSILQTMPVKIIPLSF 261 >C2FPU6.1 C2FPU6_LACPL Transcription regulator Length = 301 Score = 137 bits (346), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 86/269 (31%), Positives = 147/269 (54%), Gaps = 15/269 (5%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 MN+ L+ FV+L+ T N+++TA+ L++TQP+++ I SLE EL I LF + R+V LT A Sbjct: 1 MNLDHLRYFVALSRTENYTQTAKLLHITQPSLTKAIHSLEAELNIDLFQKAGRSVTLTPA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F D+E L LD+S+ KR ++ ++ + I T+ K LPD+ +RF + P Sbjct: 61 GKVFASDVESALLLLDKSVTNIKRFDQQ-KAPIRIAALRTL-SIKWLPDIAQRFLQLSPH 118 Query: 135 IQIQLKKSNLTREKEDLL----NQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNH 190 +Q + + T D+L N K+D+ F S+ + DI+ F+PV + + VC+ P H Sbjct: 119 AAVQFQFNTDTGLSPDILNGLRNNKYDVAFCSKMDHFSDIE--FFPVAEQTMVCITPTTH 176 Query: 191 PFANESELDIEQLNHQSIILFDEHQSPPTLKKMNRKIIENC---PDSIYTYGDTINTVHT 247 P AN S +++ + I F + L + +K+ +C P S Y + + Sbjct: 177 PLANRSSINLAETLAYPQITFSQRSG---LHPIMQKLFNDCGGQPISAYAVEED-QAIAG 232 Query: 248 MIKSDLGVSVLPDFVIGEDSEIASVPLSY 276 ++ S G++V+P+ I + + +PLS+ Sbjct: 233 LVASGFGIAVVPNMSILQTMPVKIIPLSF 261 >Q181P0.1 Q181P0_CLOD6 LysR-family transcriptional regulator Length = 293 Score = 136 bits (343), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 85/269 (31%), Positives = 141/269 (52%), Gaps = 6/269 (2%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 MN +QL+ F+S+AE L+F+KTAEK ++Q AV+ IK+LE ++ + LF R+KR V LT A Sbjct: 1 MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQINVTLFTRSKRHVELTPA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F + ++ ++ +I K ++ + + TL IG T +EK L ++ F YP Sbjct: 61 GKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIG-TLIGYEKNKLQQYLKEFSNTYPN 119 Query: 135 IQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPFAN 194 I + + + +T + N D+ F DF + V + S V +LP HP N Sbjct: 120 ISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYKTVERYSLVALLPPGHPLYN 179 Query: 195 ESELDIEQLNHQSIILFDEHQSPPTLKKM--NRKIIENCPDSIYTYGDTINTVHTMIKSD 252 E +D+ +L H I E K M NR ++ + +NT+ +++ S+ Sbjct: 180 EDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGFVPNVVQRSNNLNTIESLVSSN 239 Query: 253 LGVSVLPDFVIGE---DSEIASVPLSYTE 278 +G+S+LP F + + + +IA VP+ E Sbjct: 240 MGISILPSFCVTDTMIERDIAIVPIKEVE 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,688,562,070 Number of extensions: 68045700 Number of successful extensions: 241162 Number of sequences better than 1.0e-05: 16263 Number of HSP's gapped: 233172 Number of HSP's successfully gapped: 16263 Length of query: 307 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 172 Effective length of database: 1,788,859,178 Effective search space: 307683778616 Effective search space used: 307683778616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)