BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0021.p (586 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A9KRI0.1 A9KRI0_CLOPH ABC transporter related 776 0.0 B0N1M1.1 B0N1M1_9FIRM Putative uncharacterized protein 769 0.0 C3RIU9.1 C3RIU9_9MOLU Putative uncharacterized protein 768 0.0 Q2BE34.1 Q2BE34_9BACI Probable permease protein of ABC transport... 719 0.0 Q24QU2.1 Q24QU2_DESHY Putative uncharacterized protein 718 0.0 >A9KRI0.1 A9KRI0_CLOPH ABC transporter related Length = 613 Score = 776 bits (2004), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/576 (63%), Positives = 458/576 (79%) Query: 10 TWSKLIRYCRNHIPXXXXXXXXXXXXXXXXXXXPDQLSEMTDLITQGIAAGIDMEAIASI 69 TW KL++YC+ + PD+LSE+TDLIT+GI GID++ +A I Sbjct: 38 TWVKLLKYCKKYWAVMVIALSCVVMGTVLTLIGPDKLSELTDLITKGIVTGIDLDGVARI 97 Query: 70 GLYLVIIYGISAVLSLLQGLIMSSVTQKVSKRLRGDLSHKMNRLPISYYNKSTTGDILSR 129 G LVI YG+S +LSL+QG++M+++TQ VSK+LRGD+S K+NRLP+ +YNK+TTGD+LSR Sbjct: 98 GWTLVIFYGLSFLLSLIQGVVMATITQNVSKQLRGDISAKINRLPMWFYNKTTTGDVLSR 157 Query: 130 VTNDVDTIGQALNQSVGTLVTALSLFVGSIVMMLKTNWIMTVTAVVATIIGFGLMTVIMK 189 VTNDVDTIGQ+LNQSVGTLV+A++L VGS++MMLKTN MT+TA+VAT IGF LM +IM Sbjct: 158 VTNDVDTIGQSLNQSVGTLVSAITLLVGSLIMMLKTNVFMTITAIVATCIGFVLMMLIMG 217 Query: 190 KSQKYFQSQQEYLGKINGHVEEVYTGHTVVKAYNAEAQMRGAFEDLNRNLKNSVFKAQFL 249 KSQKYF QQ++LG+INGH+EE+Y GHT+VKAYN EA+ R F LN L+ S F +Q L Sbjct: 218 KSQKYFVRQQQHLGEINGHIEEIYDGHTIVKAYNGEAKAREKFVYLNNELRESNFMSQCL 277 Query: 250 SGMMMPIMMFIGNLGFVAVCVVGAVLATNGDISFGVIVAFMMYVRFFTXXXXXXXXXXXX 309 SG+MMP+M FIGN G+VAVCVVGAVL N ISFGVIVAF++YVR+FT Sbjct: 278 SGLMMPLMSFIGNFGYVAVCVVGAVLVMNNTISFGVIVAFILYVRYFTQPLSQLAQAAQS 337 Query: 310 XXXXXXXXKRVFEFLEAEEMDDESQKTAQLKAATGKVKFEHVQFAYEGTDNLVIRDFSTE 369 +RVFEFLEAEEM+DE KT +L+ GKV FEHV F YEG+D +I DFS Sbjct: 338 LQSAAAAGERVFEFLEAEEMEDELAKTRKLENVQGKVDFEHVNFGYEGSDKAIINDFSVS 397 Query: 370 VQPGQKIAIVGPTGAGKTTLVNLLIRFNELTGGEIYIDDTPISSLTRENIHDQFCMVLQD 429 +PGQK+AIVGPTGAGKTT+VNLL+RF+E+ G I ID+ P+ L RE++H+QFCMVLQD Sbjct: 398 TKPGQKVAIVGPTGAGKTTIVNLLMRFHEIQSGTIKIDNIPVHQLRREDVHEQFCMVLQD 457 Query: 430 TWLFEGTIRENLIYNQTGITDQDIQEACKSVGLHHFIQTLSAGYDTVLNDKVNLSAGQKQ 489 TW+FEGT+RENL+Y+ +++Q ++EACK+VGLHHFI+TL GYDT+LND+++LSAGQKQ Sbjct: 458 TWIFEGTVRENLVYSTENVSEQTLEEACKAVGLHHFIRTLPHGYDTMLNDQLSLSAGQKQ 517 Query: 490 QLTIARAMLKNAPMLILDEATSSVDTRTELLIQQAMDRLMEGRTSFVIAHRLSTIKNADV 549 QLTIARAM+ + PMLILDEATSSVDTRTEL+IQ AMD LM+GRTSF+IAHRLSTIKNAD+ Sbjct: 518 QLTIARAMIADRPMLILDEATSSVDTRTELIIQNAMDELMKGRTSFIIAHRLSTIKNADL 577 Query: 550 ILVLKDGDILESGSHSELLAKNGFYAELYNSQFEQA 585 ILV+KDGDI+ESG+H+EL+ + GFYA+LYNSQF+ A Sbjct: 578 ILVMKDGDIIESGNHNELMKQGGFYADLYNSQFDAA 613 >B0N1M1.1 B0N1M1_9FIRM Putative uncharacterized protein Length = 606 Score = 769 bits (1986), Expect = 0.0, Method: Compositional matrix adjust. Identities = 370/576 (64%), Positives = 447/576 (77%), Gaps = 1/576 (0%) Query: 10 TWSKLIRYCRNHIPXXXXXXXXXXXXXXXXXXXPDQLSEMTDLITQGIAAGIDMEAIASI 69 TW KL++YCR + PD+LSE+TDLIT GI GIDM I I Sbjct: 31 TWLKLLKYCRKYWIVMVMAIISAIFGTILTLIGPDRLSELTDLITNGIMTGIDMTRIGKI 90 Query: 70 GLYLVIIYGISAVLSLLQGLIMSSVTQKVSKRLRGDLSHKMNRLPISYYNKSTTGDILSR 129 GL LV Y SA SL QG +M++VTQ V+K LR D+S K+NRLPIS+YN ++TGDILSR Sbjct: 91 GLTLVGFYVTSAFFSLFQGFVMTTVTQWVTKNLRRDISRKINRLPISFYNHTSTGDILSR 150 Query: 130 VTNDVDTIGQALNQSVGTLVTALSLFVGSIVMMLKTNWIMTVTAVVATIIGFGLMTVIMK 189 VTND+D IGQ+LNQSVGTLV++++L GS++MMLKTN IMT TAV+AT+IGF LM VIM Sbjct: 151 VTNDIDMIGQSLNQSVGTLVSSVTLLAGSLIMMLKTNLIMTFTAVLATVIGFALMLVIMS 210 Query: 190 KSQKYFQSQQEYLGKINGHVEEVYTGHTVVKAYNAEAQMRGAFEDLNRNLKNSVFKAQFL 249 KSQKYF QQ++LG+ING+VEE+YTGHT+VKAYN E + + AF N NL+NS FKAQ L Sbjct: 211 KSQKYFARQQKHLGEINGYVEEIYTGHTIVKAYNGEVKAKEAFTRQNNNLRNSGFKAQCL 270 Query: 250 SGMMMPIMMFIGNLGFVAVCVVGAVLATNGDISFGVIVAFMMYVRFFTXXXXXXXXXXXX 309 SG+MMPIM FIGN G+VAVC++GA LA +GDISFGVIVAFMMY+R+FT Sbjct: 271 SGLMMPIMSFIGNFGYVAVCIIGAALAMSGDISFGVIVAFMMYIRYFTQPLSQLAQAAQS 330 Query: 310 XXXXXXXXKRVFEFLEAEEMDDESQKTAQLKAATGKVKFEHVQFAYEGTDNLVIRDFSTE 369 +RVFEFLEA+EM DES K +L A G V+F HV F Y+ ++ ++I DFS Sbjct: 331 LQSAAAAGERVFEFLEAQEMADESHKVKRLSKAKGGVEFRHVHFGYD-SNKIIINDFSAR 389 Query: 370 VQPGQKIAIVGPTGAGKTTLVNLLIRFNELTGGEIYIDDTPISSLTRENIHDQFCMVLQD 429 +PGQKIAIVGPTGAGKTT++NLL+RF E+ GEI ID+ P L RE++H QFCMVLQD Sbjct: 390 AEPGQKIAIVGPTGAGKTTIINLLMRFYEVDRGEIRIDNIPTKDLRREDVHGQFCMVLQD 449 Query: 430 TWLFEGTIRENLIYNQTGITDQDIQEACKSVGLHHFIQTLSAGYDTVLNDKVNLSAGQKQ 489 TWLFEGT+RENL+YN I++Q I+EACK+VGL HFI+TL GYDTVLND+VNLS GQKQ Sbjct: 450 TWLFEGTVRENLVYNTKNISNQIIEEACKAVGLDHFIRTLPYGYDTVLNDQVNLSQGQKQ 509 Query: 490 QLTIARAMLKNAPMLILDEATSSVDTRTELLIQQAMDRLMEGRTSFVIAHRLSTIKNADV 549 QLTIARAM+ + PMLILDEATSSVDTRTEL IQ+AMD LMEGRTSFVIAHRLSTIKNAD+ Sbjct: 510 QLTIARAMIADKPMLILDEATSSVDTRTELQIQKAMDELMEGRTSFVIAHRLSTIKNADL 569 Query: 550 ILVLKDGDILESGSHSELLAKNGFYAELYNSQFEQA 585 ILV+KDGDI+ESG+H +LL++ GFYAELYNSQF++A Sbjct: 570 ILVMKDGDIIESGNHEDLLSRGGFYAELYNSQFDKA 605 >C3RIU9.1 C3RIU9_9MOLU Putative uncharacterized protein Length = 625 Score = 768 bits (1982), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/576 (64%), Positives = 446/576 (77%), Gaps = 1/576 (0%) Query: 10 TWSKLIRYCRNHIPXXXXXXXXXXXXXXXXXXXPDQLSEMTDLITQGIAAGIDMEAIASI 69 TW KL++YCR + PD+LSE+TDLIT GI GIDM I I Sbjct: 50 TWLKLLKYCRKYWIVMVMAIISAIFGTILTLIGPDRLSELTDLITNGIMTGIDMTRIGKI 109 Query: 70 GLYLVIIYGISAVLSLLQGLIMSSVTQKVSKRLRGDLSHKMNRLPISYYNKSTTGDILSR 129 GL LV Y SA SL QG +M++VTQ V+K LR D+S K+NRLPIS+YN ++TGDILSR Sbjct: 110 GLTLVGFYVTSAFFSLFQGFVMATVTQWVTKNLRRDISRKINRLPISFYNHTSTGDILSR 169 Query: 130 VTNDVDTIGQALNQSVGTLVTALSLFVGSIVMMLKTNWIMTVTAVVATIIGFGLMTVIMK 189 VTND+D IGQ+LNQSVGTLV++++L GS++MMLKTN IMT TAV+AT+IGF LM VIM Sbjct: 170 VTNDIDMIGQSLNQSVGTLVSSVTLLAGSLIMMLKTNLIMTFTAVLATVIGFALMLVIMS 229 Query: 190 KSQKYFQSQQEYLGKINGHVEEVYTGHTVVKAYNAEAQMRGAFEDLNRNLKNSVFKAQFL 249 KSQKYF QQ++LG+ING+VEE+YTGHT+VKAYN E + + F N NL+NS FKAQ L Sbjct: 230 KSQKYFARQQKHLGEINGYVEEIYTGHTIVKAYNGEVKAKETFTRQNNNLRNSGFKAQCL 289 Query: 250 SGMMMPIMMFIGNLGFVAVCVVGAVLATNGDISFGVIVAFMMYVRFFTXXXXXXXXXXXX 309 SG+MMPIM FIGN G+VAVC++GA LA +GDISFGVIVAFMMY+R+FT Sbjct: 290 SGLMMPIMSFIGNFGYVAVCIIGAALAMSGDISFGVIVAFMMYIRYFTQPLSQLAQAAQS 349 Query: 310 XXXXXXXXKRVFEFLEAEEMDDESQKTAQLKAATGKVKFEHVQFAYEGTDNLVIRDFSTE 369 +RVFEFLEA+EM DES K +L A G V+F HV F Y+ ++ ++I DFS Sbjct: 350 LQSAAAAGERVFEFLEAQEMADESHKVKRLSKAKGGVEFRHVHFGYD-SNKIIINDFSAR 408 Query: 370 VQPGQKIAIVGPTGAGKTTLVNLLIRFNELTGGEIYIDDTPISSLTRENIHDQFCMVLQD 429 +PGQKIAIVGPTGAGKTT++NLL+RF E+ GEI ID+ P L RE++H QFCMVLQD Sbjct: 409 AEPGQKIAIVGPTGAGKTTIINLLMRFYEVDRGEIRIDNIPTKDLRREDVHGQFCMVLQD 468 Query: 430 TWLFEGTIRENLIYNQTGITDQDIQEACKSVGLHHFIQTLSAGYDTVLNDKVNLSAGQKQ 489 TWLFEGT+RENL+YN I++Q I+EACK+VGL HFI+TL GYDTVLND+VNLS GQKQ Sbjct: 469 TWLFEGTVRENLVYNTKNISNQIIEEACKAVGLDHFIRTLPYGYDTVLNDQVNLSQGQKQ 528 Query: 490 QLTIARAMLKNAPMLILDEATSSVDTRTELLIQQAMDRLMEGRTSFVIAHRLSTIKNADV 549 QLTIARAM+ + PMLILDEATSSVDTRTEL IQ+AMD LMEGRTSFVIAHRLSTIKNAD+ Sbjct: 529 QLTIARAMIADKPMLILDEATSSVDTRTELQIQKAMDELMEGRTSFVIAHRLSTIKNADL 588 Query: 550 ILVLKDGDILESGSHSELLAKNGFYAELYNSQFEQA 585 ILV+KDGDI+ESG+H +LL++ GFYAELYNSQF++A Sbjct: 589 ILVMKDGDIIESGNHEDLLSRGGFYAELYNSQFDKA 624 >Q2BE34.1 Q2BE34_9BACI Probable permease protein of ABC transporter system Length = 609 Score = 719 bits (1855), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/577 (57%), Positives = 434/577 (75%), Gaps = 1/577 (0%) Query: 10 TWSKLIRYCRNHIPXXXXXXXXXXXXXXXXXXXPDQLSEMTDLITQGIAAGIDMEAIASI 69 T KL+ YCR ++P PD LS +TDLI +G+ I+++ + SI Sbjct: 34 TIKKLVSYCRAYLPFIVTALVLAMAASIFSIIGPDLLSRITDLIAEGLMTEINLDEVVSI 93 Query: 70 GLYLVIIYGISAVLSLLQGLIMSSVTQKVSKRLRGDLSHKMNRLPISYYNKSTTGDILSR 129 L +YG+ + + +QG IM++VTQ+VSK LR DLS KMNR+P+ Y++ +TGD+LSR Sbjct: 94 ASILAFLYGLGFLFNYIQGFIMATVTQRVSKNLRSDLSSKMNRMPLKYFDSGSTGDVLSR 153 Query: 130 VTNDVDTIGQALNQSVGTLVTALSLFVGSIVMMLKTNWIMTVTAVVATIIGFGLMTVIMK 189 VTNDVD IGQ +NQS+ TLV+A+++F+GS+VMM TNWIM V+A+++T+IGF M+ ++ Sbjct: 154 VTNDVDLIGQTMNQSLSTLVSAITMFLGSLVMMFYTNWIMAVSAILSTLIGFVFMSFLIS 213 Query: 190 KSQKYFQSQQEYLGKINGHVEEVYTGHTVVKAYNAEAQMRGAFEDLNRNLKNSVFKAQFL 249 KSQKYF QQ LG++NGH+EE+Y+GH+VVK YN E + + F +N L S +K+QFL Sbjct: 214 KSQKYFAQQQAELGRLNGHIEEIYSGHSVVKVYNGEPEAKATFHKINTRLYESAWKSQFL 273 Query: 250 SGMMMPIMMFIGNLGFVAVCVVGAVLATNGDISFGVIVAFMMYVRFFTXXXXXXXXXXXX 309 SG+MMPIMMF+GNLG+V VCV GAVLA NG I+FGVIVAFM+Y+R FT Sbjct: 274 SGLMMPIMMFVGNLGYVVVCVAGAVLAVNGSITFGVIVAFMIYIRLFTQPLSQLAQAAAN 333 Query: 310 XXXXXXXXKRVFEFLEAEEMDDESQKTAQLKAATGKVKFEHVQFAYEGTDNLVIRDFSTE 369 +RVFEFL +E+++E K L G+V+F++V+F Y D ++I DFS Sbjct: 334 LQATAAASERVFEFLAEDELENEDNKKTVLNDVKGEVEFKNVRFGYS-EDKMIINDFSAL 392 Query: 370 VQPGQKIAIVGPTGAGKTTLVNLLIRFNELTGGEIYIDDTPISSLTRENIHDQFCMVLQD 429 + GQK+AIVGPTGAGKTT++NLL+RF E++GGEI ID P L+RE + FCMVLQD Sbjct: 393 AKSGQKVAIVGPTGAGKTTIINLLMRFYEVSGGEILIDGVPTKELSREKLRSLFCMVLQD 452 Query: 430 TWLFEGTIRENLIYNQTGITDQDIQEACKSVGLHHFIQTLSAGYDTVLNDKVNLSAGQKQ 489 TWLFEGTIREN++Y++ G++DQ++ EA K+VGLHHFIQTL GYDT+L+DK NLSAGQKQ Sbjct: 453 TWLFEGTIRENIVYSRKGVSDQEVAEAAKAVGLHHFIQTLPMGYDTILDDKANLSAGQKQ 512 Query: 490 QLTIARAMLKNAPMLILDEATSSVDTRTELLIQQAMDRLMEGRTSFVIAHRLSTIKNADV 549 +TIARAM+K+AP+LILDEATSSVDTRTE+LIQ+AMD+L G+TSFVIAHRLSTIKNAD+ Sbjct: 513 LITIARAMVKDAPLLILDEATSSVDTRTEVLIQEAMDKLTAGKTSFVIAHRLSTIKNADL 572 Query: 550 ILVLKDGDILESGSHSELLAKNGFYAELYNSQFEQAS 586 ILV+KDGDI+ESG+H ELL +NGFYA+LYNSQFE AS Sbjct: 573 ILVMKDGDIIESGNHEELLGQNGFYADLYNSQFEDAS 609 >Q24QU2.1 Q24QU2_DESHY Putative uncharacterized protein Length = 617 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/586 (58%), Positives = 437/586 (74%), Gaps = 10/586 (1%) Query: 10 TWSKLIRYCRNHIPXXXXXXXXXXXXXXXXXXXPDQLSEMTDLITQGIAA---------G 60 T KL+RYC+ ++P PD++ ++T+ I +G+ A Sbjct: 33 TMGKLLRYCQQYLPAMVIALLFAAIGTLLQIIGPDKIKDLTNEIAKGLPALLNNVPVLNS 92 Query: 61 IDMEAIASIGLYLVIIYGISAVLSLLQGLIMSSVTQKVSKRLRGDLSHKMNRLPISYYNK 120 IDM A+A+I LV+ Y S +LS +Q +M++VTQKVSK++R D+S K+NRLP+ Y+++ Sbjct: 93 IDMTAVANIAWLLVLFYATSMLLSFVQSYMMATVTQKVSKKMRSDISQKINRLPLKYFDQ 152 Query: 121 STTGDILSRVTNDVDTIGQALNQSVGTLVTALSLFVGSIVMMLKTNWIMTVTAVVATIIG 180 ++ GD+LSRVTNDVD IGQ LNQS+G+LVTA+++FVG+++MM NWI+ +TA+ A+++G Sbjct: 153 TSYGDVLSRVTNDVDAIGQTLNQSIGSLVTAITMFVGALIMMFYNNWILALTAIGASLLG 212 Query: 181 FGLMTVIMKKSQKYFQSQQEYLGKINGHVEEVYTGHTVVKAYNAEAQMRGAFEDLNRNLK 240 F LMT IM KSQK+F SQQ LG INGH+EE+Y GH VVK YN + FED+N L Sbjct: 213 FILMTFIMSKSQKHFVSQQNGLGAINGHIEEMYAGHNVVKVYNHSKASKATFEDINGKLY 272 Query: 241 NSVFKAQFLSGMMMPIMMFIGNLGFVAVCVVGAVLATNGDISFGVIVAFMMYVRFFTXXX 300 NS +K+QFLSG+MMP+M F+GNLG+VAVCVVGAVLA NG I+FGVIVAFM+Y+R FT Sbjct: 273 NSAWKSQFLSGLMMPLMSFVGNLGYVAVCVVGAVLAMNGTITFGVIVAFMIYIRLFTQPL 332 Query: 301 XXXXXXXXXXXXXXXXXKRVFEFLEAEEMDDESQKTAQLKAATGKVKFEHVQFAYEGTDN 360 +RVFEFL EM DE K +L+ G V+F HV+F Y D Sbjct: 333 SQIAQAFNNLQRTAAASERVFEFLGEAEMADERDKVKRLEDVRGDVEFSHVKFGYS-PDK 391 Query: 361 LVIRDFSTEVQPGQKIAIVGPTGAGKTTLVNLLIRFNELTGGEIYIDDTPISSLTRENIH 420 +I DFS +V+ GQKIAIVGPTGAGKTT++NLL+RF EL GGEI ID TPIS + REN+H Sbjct: 392 TIIHDFSAKVKAGQKIAIVGPTGAGKTTIINLLMRFYELDGGEILIDGTPISQVHRENVH 451 Query: 421 DQFCMVLQDTWLFEGTIRENLIYNQTGITDQDIQEACKSVGLHHFIQTLSAGYDTVLNDK 480 +QF MVLQDTWLFEGTI+EN+IY+Q G+TD+ + ACK+VGLHHFI TL GY+TV+ND Sbjct: 452 EQFSMVLQDTWLFEGTIKENIIYSQQGVTDEQVIAACKAVGLHHFISTLPEGYNTVINDN 511 Query: 481 VNLSAGQKQQLTIARAMLKNAPMLILDEATSSVDTRTELLIQQAMDRLMEGRTSFVIAHR 540 V+LS GQKQ +TIARAM+ + P+LILDEATSSVDTRTE ++Q+AMD+L G TSFVIAHR Sbjct: 512 VSLSEGQKQLITIARAMIADTPLLILDEATSSVDTRTERIVQEAMDKLTRGCTSFVIAHR 571 Query: 541 LSTIKNADVILVLKDGDILESGSHSELLAKNGFYAELYNSQFEQAS 586 LSTIKNAD+ILV+KDGDI+ESG+H +LLAKNGFYAELYNSQFEQ++ Sbjct: 572 LSTIKNADLILVMKDGDIIESGNHEQLLAKNGFYAELYNSQFEQSA 617 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,870,231,609 Number of extensions: 106726165 Number of successful extensions: 728970 Number of sequences better than 1.0e-05: 60220 Number of HSP's gapped: 671389 Number of HSP's successfully gapped: 63680 Length of query: 586 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 445 Effective length of database: 1,732,333,088 Effective search space: 770888224160 Effective search space used: 770888224160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)