BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0011.p (438 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5KUP7.1 Q5KUP7_GEOKA Mannose-6-phosphate isomerase (Phosphomann... 303 2e-80 Q6T1V9.1 Q6T1V9_ANETH Mannose-1-phosphate guanylyltransferase EC... 296 3e-78 C2SUK4.1 C2SUK4_BACCE Mannose-1-phosphate guanylyltransferase 290 2e-76 B3KE79.1 B3KE79_9BACI Mannose-1-phosphate guanylyltransferase (G... 288 1e-75 C2YZ97.1 C2YZ97_BACCE Mannose-1-phosphate guanylyltransferase 287 2e-75 >Q5KUP7.1 Q5KUP7_GEOKA Mannose-6-phosphate isomerase (Phosphomannose isomerase) ; mannose-1-phosphate guanylyl transferase (GDP-mannose pyrophosphorylase) EC=2.7.7.22 EC=5.3.1.8 Length = 458 Score = 303 bits (776), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 162/436 (37%), Positives = 240/436 (55%), Gaps = 3/436 (0%) Query: 4 ILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 +LL GGSG++ RSK FL++L +G +SM+ RV QL + GL SA+ + Sbjct: 4 VLLSGGSGKRLWPLSNDARSKQFLKVLETQNGELQSMVQRVWEQLGNVGLADSAVIATSK 63 Query: 63 DQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYTDDA 122 Q+ + + P+I EP +R TF + ++ + V P D Y +D Sbjct: 64 SQVDMIQSQLGQDVPIIIEPMRRDTFPAIALASVYLYSVEGIRLDEVVAVLPVDPYVEDR 123 Query: 123 FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGN-AGPGAYAPVLSFQEKPDSA 181 FF + + S A+L L+G PT+PS +YGYI+P + + Y V F EKP Sbjct: 124 FFHRVKDLEETVLASGADLALIGVEPTYPSAKYGYIVPASKSSDSGYLRVSHFTEKPTEE 183 Query: 182 RALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAERS 241 +A ELI+Q ALWNCG+FAF+L +++S L+ G P + + Y P SFD EV E++ Sbjct: 184 KAAELIKQGALWNCGVFAFKLDYIISLLQEKGFPIQYDELLKQYDKLPKISFDYEVVEKT 243 Query: 242 RKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVSGI 301 VVL ++G W+DLG+W+ LT +A IG+G I +TH++N+L IP+ V+G+S Sbjct: 244 EHIVVLPYDGYWKDLGTWNTLTEEMATNQIGKGVISADCENTHLINELDIPVTVLGISNA 303 Query: 302 VAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRT-FSGPELILTLAL 360 V A SP+GIL+A K ++ IKE++ + P Y E WG Y VLD T F +LT + Sbjct: 304 VVAVSPDGILVADKAKSPKIKELVNDFDQRPMYEERRWGWYRVLDYTKFEDGREVLTKRI 363 Query: 361 SLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAFTAL 420 + G +L H S+ W I+ G+GE LNG + G + I G +HGI+A T L Sbjct: 364 GVTAGKNLSYQMHHHRSEVWTIIKGEGEFALNGEIRRVKPGDVLEIPVGAKHGIKAITDL 423 Query: 421 KLIEIRTCGQDDEQKI 436 + IE++T Q E+ I Sbjct: 424 EFIEVQTGTQLIEEDI 439 >Q6T1V9.1 Q6T1V9_ANETH Mannose-1-phosphate guanylyltransferase EC=2.7.7.13 Length = 464 Score = 296 bits (757), Expect = 3e-78, Method: Compositional matrix adjust. Identities = 160/439 (36%), Positives = 238/439 (54%), Gaps = 7/439 (1%) Query: 4 ILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 ILL GGSG++ RSK FL++L + ESM+ RV QL GL + + Sbjct: 4 ILLSGGSGKRLWPLSNDSRSKQFLKVLKNDNNEFESMVQRVWNQLHTVGLAEDSYVATSK 63 Query: 63 DQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYTDDA 122 Q+ + P+I EP +R TF + +D + V P D Y + Sbjct: 64 SQVDMLHAQLGTEVPLIIEPERRDTFPAIALAATYLYSMEGVREDDVISVLPVDPYVESR 123 Query: 123 FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVLS---FQEKPD 179 FFD L S AEL L+G +PT PS++YGYI+P A VL+ F EKP Sbjct: 124 FFDRVKDLETALVQSSAELALIGVKPTFPSEKYGYIVPKKGEHDVVANVLAVDYFVEKPS 183 Query: 180 SARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAE 239 ++RA EL++QQA WNCGIFAF+L +++S L+ LP + Y P SFD EV E Sbjct: 184 TSRAQELMKQQAFWNCGIFAFKLGYLISLLKEKKLPTDYEEMLHTYNQLPKISFDYEVVE 243 Query: 240 RSRKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVS 299 +++ ++L +EGEW+DLG+W+ LT + ++G+G I +TH++N+L IP+ ++G+S Sbjct: 244 KAQNIIMLPYEGEWKDLGTWNTLTEEMNTNLLGKGIISEGCENTHLINELDIPVTILGIS 303 Query: 300 GIVAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRTF--SGPELILT 357 V A SP+GIL+ K + IK+I++ + P Y E WG Y VLD T +G E +LT Sbjct: 304 NAVIAASPDGILVTDKEASPRIKDIMKNFNQRPMYEERRWGWYRVLDHTKVENGNE-VLT 362 Query: 358 LALSLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAF 417 + + G +L S+ W I+SG+G+ VLN + G + I G +H IRA Sbjct: 363 KRIGVHAGKNLSYQIHHKRSEVWTIISGEGQFVLNDQLYHVKPGDVLQIPVGAKHAIRAI 422 Query: 418 TALKLIEIRTCGQDDEQKI 436 T L+ +E++T + E+ I Sbjct: 423 TDLQFVEVQTGSELIEEDI 441 >C2SUK4.1 C2SUK4_BACCE Mannose-1-phosphate guanylyltransferase Length = 461 Score = 290 bits (742), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 154/426 (36%), Positives = 232/426 (54%), Gaps = 2/426 (0%) Query: 4 ILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 ILL GGSG++ RSK FL++L + +G ++SM+ RV QL+ GL SA+ + Sbjct: 4 ILLSGGSGKRLWPLSNDTRSKQFLKVLESEEGTRQSMVQRVWGQLESVGLNESAVIATSK 63 Query: 63 DQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYTDDA 122 Q + P+I EP +R TF ++T+ V P D Y +D+ Sbjct: 64 TQTEMIHNQLGMNVPLIVEPERRDTFPAIALASAFLFSEQHTNLDETVIVLPVDPYVEDS 123 Query: 123 FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGN-AGPGAYAPVLSFQEKPDSA 181 FF+ L +S A+L L+G PT+PS +YGYI+P A Y V F EKP Sbjct: 124 FFERVKDLEAALQNSNADLALMGVEPTYPSSKYGYIVPTEEATTCEYFNVSRFTEKPSEE 183 Query: 182 RALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAERS 241 +A+ LI+Q+ALWNCG+FAF+L ++++ LE LP +E Y P SFD EV E++ Sbjct: 184 KAVTLIEQKALWNCGVFAFKLRYLINILEEKNLPILYHDLSERYSELPKISFDYEVVEKA 243 Query: 242 RKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVSGI 301 V L + G W+DLG+W+ LT +A IG+G I S++TH+VN+L +P+ V+G+S I Sbjct: 244 NHIVALPYSGYWKDLGTWNTLTEEMATTQIGKGVISEDSQNTHVVNELEVPVTVVGLSDI 303 Query: 302 VAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRTFSGPELILTLALS 361 V A SP+GIL++ K + IK+++ + P Y E WG VLD E ++T + Sbjct: 304 VVAASPDGILVSDKASSPKIKDLIGSFNQPPMYEERIWGWSKVLDYAKYENEEMVTKRIC 363 Query: 362 LLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAFTALK 421 + D ++ W I+ G+GE++L + G I I G +H IRA + L+ Sbjct: 364 IEENKDSTYHYHNLRNEVWTIIRGEGELILEDHIRRVKAGDIVHIPAGKKHAIRALSELE 423 Query: 422 LIEIRT 427 IE++T Sbjct: 424 FIEVQT 429 >B3KE79.1 B3KE79_9BACI Mannose-1-phosphate guanylyltransferase (GDP) EC=2.7.7.22 Length = 454 Score = 288 bits (736), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 151/439 (34%), Positives = 236/439 (53%), Gaps = 6/439 (1%) Query: 1 VHTILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFV 59 + ILL GGSG++ RSK FL++LP +G ESM+ RV +QL + L S+ Sbjct: 1 MKIILLSGGSGKRLWPLSNELRSKQFLKVLPGEEGESESMVQRVWKQLQRSNLADSSFIA 60 Query: 60 AHEDQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYT 119 A Q+ + R R P+I EP +R TF ++T+ V P D + Sbjct: 61 AGISQIEIIRNQLPGRVPIIVEPQRRDTFPAIALAAVYLYSIANVNLQETVTVLPVDPFV 120 Query: 120 DDAFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNA--GPGAYAPVLSFQEK 177 ++ FF +L +S A++ L+G +PT+PS++YGYI+P G Y V SF+EK Sbjct: 121 EEPFFHTLSSLDRVLEESGADIALMGVKPTYPSEKYGYILPEQTEDASGGYRNVKSFKEK 180 Query: 178 PDSARALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEV 237 P +A + I Q A WNCG+F+F+L +++ L GLP + ++ Y SFD EV Sbjct: 181 PSEEQASQYIAQSAYWNCGVFSFKLDYLIQLLIDKGLPIQYEEMSKQYARLSKISFDYEV 240 Query: 238 AERSRKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIG 297 E++++ VV+ +EG W+DLG+W+ LT + P+ G+G I +TH++N+L IP+ V+G Sbjct: 241 IEKAQRVVVVPYEGSWKDLGTWNTLTEEIPSPINGKGTISDDCYNTHLINELDIPVAVLG 300 Query: 298 VSGIVAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLD--RTFSGPELI 355 +S +V A +GIL+ K + +KE + + P Y E WG Y VLD + +G E + Sbjct: 301 LSNVVVATGADGILVTEKSSSPRLKEFISHIEQRPMYEERRWGWYRVLDLQKHKNGHE-V 359 Query: 356 LTLALSLLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIR 415 LT + + G + H + W I SG GE+ + + Q G + I G HGIR Sbjct: 360 LTKRIRIDAGKNSSYHYHLHREEYWTITSGTGEVCIQDQVKSVQAGDVLKIPAGTLHGIR 419 Query: 416 AFTALKLIEIRTCGQDDEQ 434 A L+ IE+++ + E+ Sbjct: 420 ALEDLEFIEVQSGTEVSEE 438 >C2YZ97.1 C2YZ97_BACCE Mannose-1-phosphate guanylyltransferase Length = 461 Score = 287 bits (734), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 151/426 (35%), Positives = 234/426 (54%), Gaps = 2/426 (0%) Query: 4 ILLCGGSGQKXX-XXXXXRSKMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 ILL GGSG++ RSK FL++L + +G ++SM+ RV QL+ GL S++ + Sbjct: 4 ILLSGGSGKRLWPLSNDTRSKQFLKVLESEEGARQSMVQRVWGQLESVGLHESSVIATSK 63 Query: 63 DQLALTRRHTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYTDDA 122 Q + P+I EP +R TF ++T+ V P D Y +D+ Sbjct: 64 TQTEMIHNQLGMDVPLIVEPERRDTFPAIALASAFLFSEQHTSLDETVIVLPVDPYVEDS 123 Query: 123 FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGN-AGPGAYAPVLSFQEKPDSA 181 FF+ L +S A+L L+G PT+PS +YGYI+P A Y V F EKP Sbjct: 124 FFERVKDLELALQNSDADLALMGVEPTYPSSKYGYIVPTEEANTCEYFNVSRFTEKPSEE 183 Query: 182 RALELIQQQALWNCGIFAFRLSFMLSHLERMGLPAEPAAFTELYPGFPVRSFDQEVAERS 241 +A+ LI+Q+ALWNCG+FAF+LS++++ LE LP ++ Y P SFD EV E++ Sbjct: 184 KAVTLIEQKALWNCGVFAFKLSYLINILEEKNLPILYHDLSKHYSDLPKISFDYEVVEKA 243 Query: 242 RKAVVLRHEGEWRDLGSWDALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGVSGI 301 + V L + G W+DLG+W+ LT +A IG+G I S++TH+VN+L +P+ V+G+S + Sbjct: 244 KHIVALPYSGYWKDLGTWNTLTEEMATMQIGKGVISEDSKNTHVVNELEVPVTVVGLSDV 303 Query: 302 VAAGSPEGILIAGKGEANAIKEILQQAAEEPRYGETGWGSYTVLDRTFSGPELILTLALS 361 V A SP+GIL++ K + IK+++ + P Y E WG VLD E ++T + Sbjct: 304 VVAASPDGILVSDKASSPKIKDLIGSFNQPPMYEERIWGWSKVLDYAKYENEEMVTKRIC 363 Query: 362 LLPGHDLPELQCQHSSKSWVIVSGQGEIVLNGLPAAAQTGGIFTIARGDRHGIRAFTALK 421 + + ++ W I+ G+GE++L + G I I G +H IRA + L+ Sbjct: 364 IEENQNSTYHYHNLRNEVWTIIRGEGELILEDHIRRVKAGDIVHIPAGKKHAIRALSELE 423 Query: 422 LIEIRT 427 IE++T Sbjct: 424 FIEVQT 429 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,691,348,644 Number of extensions: 116639527 Number of successful extensions: 268840 Number of sequences better than 1.0e-05: 1159 Number of HSP's gapped: 266332 Number of HSP's successfully gapped: 1164 Length of query: 438 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 299 Effective length of database: 1,751,175,118 Effective search space: 523601360282 Effective search space used: 523601360282 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)