BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0004.p (196 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4IH77.1 C4IH77_CLOBU SNARE protein 203 5e-51 B1QXZ8.1 B1QXZ8_CLOBU Snare associated golgi protein 203 5e-51 B9MQM9.1 B9MQM9_ANATD SNARE associated Golgi protein 171 3e-41 A4XJH2.1 A4XJH2_CALS8 Putative uncharacterized protein 155 1e-36 Q0AVR3.1 Q0AVR3_SYNWW Putative uncharacterized protein 146 7e-34 >C4IH77.1 C4IH77_CLOBU SNARE protein Length = 225 Score = 203 bits (517), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 109/191 (57%), Positives = 149/191 (78%) Query: 1 VSFDEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGM 60 VS DEF KYILS G +G +LI FQILQTV+APIPGEV+QIAGGY+YG+ LGT+Y G+ Sbjct: 34 VSLDEFNKYILSMGNSGVFMLIFFQILQTVVAPIPGEVIQIAGGYVYGLPLGTLYSLIGL 93 Query: 61 LTGSAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVY 120 + GS IAFYFTR +GG I L+ KKKLKWM D+M + F + LF+ F++PGLPKD ++Y Sbjct: 94 IIGSGIAFYFTRLIGGDFIADLLRKKKLKWMLDIMESRKFEIILFIIFIMPGLPKDFMIY 153 Query: 121 IAGLTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYK 180 +AGLT ++P++ F IL+A RLPW++ASV+VGAN+ NY TII+ ++A++ F++G+ YK Sbjct: 154 VAGLTTLRPMKFFMILIASRLPWIMASVSVGANINAGNYLLTIIVLIIAIIGFIIGIFYK 213 Query: 181 DKLIQRFGAGK 191 DK+I++F K Sbjct: 214 DKIIKKFTNEK 224 >B1QXZ8.1 B1QXZ8_CLOBU Snare associated golgi protein Length = 225 Score = 203 bits (517), Expect = 5e-51, Method: Compositional matrix adjust. Identities = 109/191 (57%), Positives = 149/191 (78%) Query: 1 VSFDEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGM 60 VS DEF KYILS G +G +LI FQILQTV+APIPGEV+QIAGGY+YG+ LGT+Y G+ Sbjct: 34 VSLDEFNKYILSMGNSGVFMLIFFQILQTVVAPIPGEVIQIAGGYVYGLPLGTLYSLIGL 93 Query: 61 LTGSAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVY 120 + GS IAFYFTR +GG I L+ KKKLKWM D+M + F + LF+ F++PGLPKD ++Y Sbjct: 94 IIGSGIAFYFTRLIGGDFIADLLRKKKLKWMLDIMESRKFEIILFIIFIMPGLPKDFMIY 153 Query: 121 IAGLTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYK 180 +AGLT ++P++ F IL+A RLPW++ASV+VGAN+ NY TII+ ++A++ F++G+ YK Sbjct: 154 VAGLTTLRPMKFFMILIASRLPWIMASVSVGANINAGNYLLTIIVLIIAIIGFIIGIFYK 213 Query: 181 DKLIQRFGAGK 191 DK+I++F K Sbjct: 214 DKIIKKFTNEK 224 >B9MQM9.1 B9MQM9_ANATD SNARE associated Golgi protein Length = 236 Score = 171 bits (433), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 82/182 (45%), Positives = 124/182 (68%) Query: 4 DEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTG 63 +F+ ++ S G+ G V IL QILQ +I+ IPGE VQI+GGY+YG LGT+Y G++ G Sbjct: 41 QKFKSWVWSFGQLGVLVFILTQILQVIISAIPGEAVQISGGYLYGTLLGTVYSLIGIMIG 100 Query: 64 SAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAG 123 S FY TR +G L+ +++ ++KL+ ++ + +F+ FLIPGLPKD+L YIAG Sbjct: 101 SVCVFYITRLLGYSLVRKIVSEEKLRKFYSLINSPKGEIAIFLLFLIPGLPKDILTYIAG 160 Query: 124 LTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKL 183 L+P+KPLR F I+ RLP + S +G++L +KNYT I++S A + FVLG++Y+D + Sbjct: 161 LSPIKPLRFFAIVAIARLPGIFFSSYIGSSLEEKNYTMAIVVSAAATILFVLGVVYRDNI 220 Query: 184 IQ 185 I+ Sbjct: 221 IK 222 >A4XJH2.1 A4XJH2_CALS8 Putative uncharacterized protein Length = 232 Score = 155 bits (393), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 83/192 (43%), Positives = 123/192 (64%) Query: 4 DEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTG 63 +FR ++LS GK G V ILFQ+LQ V++ IPGE VQ++GGYIYG GT Y G++ G Sbjct: 41 SKFRDWVLSYGKWGVLVFILFQVLQVVVSVIPGEAVQVSGGYIYGTFAGTFYSLTGIMIG 100 Query: 64 SAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAG 123 S I FY +R +G LI +++ +K L+ ++ +F+ FLIPG+PKD+LVYIAG Sbjct: 101 SVIVFYLSRLLGYNLIKKVVSEKSLEKFYFVINSPKIETVIFLLFLIPGIPKDILVYIAG 160 Query: 124 LTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKL 183 L+P+KPL F I R P + S G+NL +KNY I ++V+A+V F++GL+Y +K+ Sbjct: 161 LSPIKPLNFFIITAIARFPGIFFSSYFGSNLEKKNYEVAIAVAVVAIVLFIIGLVYHEKI 220 Query: 184 IQRFGAGKRAGR 195 ++ + R Sbjct: 221 LKMISSLVHKKR 232 >Q0AVR3.1 Q0AVR3_SYNWW Putative uncharacterized protein Length = 224 Score = 146 bits (369), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 75/182 (41%), Positives = 121/182 (66%) Query: 4 DEFRKYILSTGKAGPAVLILFQILQTVIAPIPGEVVQIAGGYIYGVTLGTIYVTAGMLTG 63 ++FR YIL G V ILFQ++Q +IA IPGE++QIAGG++YG G+IY AG+L G Sbjct: 41 EQFRDYILGFGSQNVLVFILFQVVQVIIAAIPGELLQIAGGFVYGTWWGSIYSLAGILLG 100 Query: 64 SAIAFYFTRFMGGFLIGRLIEKKKLKWMTDMMGRKNFSVFLFVFFLIPGLPKDLLVYIAG 123 S +AFY +R +G L+ ++ +KLK + ++ ++ + +F+ FLIPGLPKD++ Y+ G Sbjct: 101 SIVAFYISRLLGYPLVCYMVAPQKLKRFSRLLRQQKSEISIFILFLIPGLPKDVITYVGG 160 Query: 124 LTPMKPLRLFGILLAGRLPWLIASVAVGANLYQKNYTSTIILSVLAVVAFVLGLIYKDKL 183 LTP+ P R I + R P ++ S +GA++ Q+ Y ++ S+ A++ F+ GL+ KD+L Sbjct: 161 LTPLNPFRFLLIAIIARFPGILGSAYLGASVEQQAYKEALLFSIAALLLFLAGLLLKDRL 220 Query: 184 IQ 185 + Sbjct: 221 FK 222 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.330 0.147 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,150,690,651 Number of extensions: 47479081 Number of successful extensions: 185164 Number of sequences better than 1.0e-05: 552 Number of HSP's gapped: 185317 Number of HSP's successfully gapped: 552 Length of query: 196 Length of database: 3,060,696,203 Length adjustment: 129 Effective length of query: 67 Effective length of database: 1,845,385,268 Effective search space: 123640812956 Effective search space used: 123640812956 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 127 (53.5 bits)