BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0001.p (408 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 557 e-157 C5DA98.1 C5DA98_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 556 e-156 A4IMW7.1 A4IMW7_GEOTN Malic enzyme EC=1.1.1.38 555 e-156 B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarbo... 555 e-156 Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 555 e-156 >P16468.1 MAOX_BACST NAD-dependent malic enzyme EC=1.1.1.38 Length = 478 Score = 557 bits (1436), Expect = e-157, Method: Compositional matrix adjust. Identities = 268/398 (67%), Positives = 320/398 (80%) Query: 3 QAVYQLNGGTIVELLDEIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQ 62 +A+ ++ G IV + D L+H GGK+ET K P++T++DLS+VYTPGVA VC AIA+D Sbjct: 70 EALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDP 129 Query: 63 AQAYELTTKKNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTK 122 +AY LT K+NTVAVVSDGTAVLGLGDIGP AAMPVMEGKA LFK+FA VDA PICLDTK Sbjct: 130 RKAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLDTK 189 Query: 123 GTEEIIAIVKALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGL 182 TEEII IVKA+AP FGGINLEDIS+PRCFEIEKRL++ELDIPVFHDDQHGTA+V+LAGL Sbjct: 190 DTEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGL 249 Query: 183 LNALKVCGKNIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHW 242 LNALK+ K ++D+K+V+ GIGAAGI+ +K+LL AG N+IGVDR GA++R Y +W Sbjct: 250 LNALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYW 309 Query: 243 NEYARMTNPCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEID 302 EYA++TNP G LSDVI GADVFIGVSAP +LK+EDV MA+DPIVFAMANP PEID Sbjct: 310 QEYAQLTNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEID 369 Query: 303 PAIAAPHVRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVID 362 P +A P+VRVMATGRSDYPNQINNVLCFPGIFRGALDC A +IN+EMKLAAA+AI+SV+ Sbjct: 370 PELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVT 429 Query: 363 AADLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARK 400 +L+ETYIIP F+ + +G ARK Sbjct: 430 EDELNETYIIPSVFNSKVVERVRQAVVEAAYRTGVARK 467 >C5DA98.1 C5DA98_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 478 Score = 556 bits (1433), Expect = e-156, Method: Compositional matrix adjust. Identities = 266/397 (67%), Positives = 320/397 (80%) Query: 4 AVYQLNGGTIVELLDEIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQA 63 A+ ++ G I+ + D +H GGK+ET K PI+T++DLS+VYTPGVA VC+AIA+D Sbjct: 71 ALKKIQGVKIIHVSDRTFWMHIGGKIETNSKFPIKTRDDLSRVYTPGVARVCKAIAEDPK 130 Query: 64 QAYELTTKKNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKG 123 +AY LT K+NTVAV+SDGTAVLGLG+IGP AAMPVMEGKA LFKQFA VDA PICLDTK Sbjct: 131 KAYSLTIKRNTVAVISDGTAVLGLGNIGPYAAMPVMEGKAMLFKQFAGVDAFPICLDTKD 190 Query: 124 TEEIIAIVKALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLL 183 TEEII IVKA+AP FGGINLEDIS+P CFEIE+RL++ELDIPVFHDDQHGTA+V+LAGLL Sbjct: 191 TEEIIQIVKAIAPAFGGINLEDISAPHCFEIERRLKEELDIPVFHDDQHGTAVVLLAGLL 250 Query: 184 NALKVCGKNIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHWN 243 NALK+ GK ++D+K+VV GIGAAGI+ +K+LL AG N+IGVDR GA++R Y +W Sbjct: 251 NALKIVGKKLEDIKVVVTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDEKYDNPYWQ 310 Query: 244 EYARMTNPCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDP 303 EYA++TNP E G LS+VI GADVFIGVSAP +LK++DV MAKDPIVFAMANP PEIDP Sbjct: 311 EYAQITNPDNEKGSLSEVIAGADVFIGVSAPGILKVDDVKKMAKDPIVFAMANPVPEIDP 370 Query: 304 AIAAPHVRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVIDA 363 +A P+VRVMATGRSDYPNQINNVLCFPGIFRGALDC A +IN+EMKLAAA+AI+SV+ Sbjct: 371 ELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVTD 430 Query: 364 ADLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARK 400 +L+ETYIIP F+ + +G ARK Sbjct: 431 DELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 467 >A4IMW7.1 A4IMW7_GEOTN Malic enzyme EC=1.1.1.38 Length = 427 Score = 555 bits (1431), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/398 (67%), Positives = 319/398 (80%) Query: 3 QAVYQLNGGTIVELLDEIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQ 62 +A+ ++ G IV + D L+H GGK+ET K P++T++DLS+VYTPGVA VC AIA+D Sbjct: 19 EALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDP 78 Query: 63 AQAYELTTKKNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTK 122 +AY LT K+NTVAVVSDGTAVLGLGDIGP AAMPVMEGKA LFKQFA VDA P+CLDTK Sbjct: 79 RKAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKQFAGVDAFPVCLDTK 138 Query: 123 GTEEIIAIVKALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGL 182 TEEII IVKA+AP FGGINLEDIS+PRCFEIE RL++ELDIPVFHDDQHGTA+V+LAGL Sbjct: 139 DTEEIIQIVKAIAPAFGGINLEDISAPRCFEIEARLKEELDIPVFHDDQHGTAVVLLAGL 198 Query: 183 LNALKVCGKNIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHW 242 LNALK+ K ++D+K+V+ GIGAAGI+ +K+LL AG N+IGVDR GA++R Y +W Sbjct: 199 LNALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRYGAIHRDETYENPYW 258 Query: 243 NEYARMTNPCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEID 302 EYA++TNP G LSDVI GADVFIGVSAP +LK+EDV MA+DPIVFAMANP PEID Sbjct: 259 QEYAQITNPYNLKGSLSDVIVGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPVPEID 318 Query: 303 PAIAAPHVRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVID 362 P +A P+VRVMATGRSDYPNQINNVLCFPGIFRGALDC A +IN+EMKLAAA+AI+SV+ Sbjct: 319 PELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVT 378 Query: 363 AADLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARK 400 +L+ETYIIP F+ + +G ARK Sbjct: 379 EDELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 416 >B4BKJ0.1 B4BKJ0_9BACI Malate dehydrogenase (Oxaloacetate-decarboxylating) EC=1.1.1.38 Length = 470 Score = 555 bits (1431), Expect = e-156, Method: Compositional matrix adjust. Identities = 267/398 (67%), Positives = 319/398 (80%) Query: 3 QAVYQLNGGTIVELLDEIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQ 62 +A+ ++ G IV + D L+H GGK+ET K P++T++DLS+VYTPGVA VC AIA+D Sbjct: 62 EALKKIRGVKIVNVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDP 121 Query: 63 AQAYELTTKKNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTK 122 +AY LT K+NTVAVVSDGTAVLGLGDIGP AAMPVMEGKA LFKQFA VDA P+CLDTK Sbjct: 122 RKAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKQFAGVDAFPVCLDTK 181 Query: 123 GTEEIIAIVKALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGL 182 TEEII IVKA+AP FGGINLEDIS+PRCFEIE RL++ELDIPVFHDDQHGTA+V+LAGL Sbjct: 182 DTEEIIQIVKAIAPAFGGINLEDISAPRCFEIEARLKEELDIPVFHDDQHGTAVVLLAGL 241 Query: 183 LNALKVCGKNIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHW 242 LNALK+ K ++D+K+V+ GIGAAGI+ +K+LL AG N+IGVDR GA++R Y +W Sbjct: 242 LNALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRYGAIHRDETYENPYW 301 Query: 243 NEYARMTNPCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEID 302 EYA++TNP G LSDVI GADVFIGVSAP +LK+EDV MA+DPIVFAMANP PEID Sbjct: 302 QEYAQITNPDNLKGSLSDVIVGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPVPEID 361 Query: 303 PAIAAPHVRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVID 362 P +A P+VRVMATGRSDYPNQINNVLCFPGIFRGALDC A +IN+EMKLAAA+AI+SV+ Sbjct: 362 PELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAKEINEEMKLAAAKAIASVVT 421 Query: 363 AADLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARK 400 +L+ETYIIP F+ + +G ARK Sbjct: 422 EDELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 459 >Q5L011.1 Q5L011_GEOKA Malic enzyme EC=1.1.1.38 Length = 478 Score = 555 bits (1430), Expect = e-156, Method: Compositional matrix adjust. Identities = 266/398 (66%), Positives = 320/398 (80%) Query: 3 QAVYQLNGGTIVELLDEIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQ 62 +A+ ++ G I+ + D L+H GGK+ET K P++T++DLS+VYTPGVA VC AIA+D Sbjct: 70 EALKKIQGVKIINVSDRTFLMHIGGKIETNSKIPVKTRDDLSRVYTPGVARVCTAIAEDP 129 Query: 63 AQAYELTTKKNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTK 122 +AY LT K+NTVAVVSDGTAVLGLGDIGP AAMPVMEGKA LFK+FA VDA PICL+TK Sbjct: 130 RKAYSLTIKRNTVAVVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGVDAFPICLNTK 189 Query: 123 GTEEIIAIVKALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGL 182 TEEII IVKA+AP FGGINLEDIS+PRCFEIEKRL++ELDIPVFHDDQHGTA+V+LAGL Sbjct: 190 DTEEIIQIVKAIAPAFGGINLEDISAPRCFEIEKRLKEELDIPVFHDDQHGTAVVLLAGL 249 Query: 183 LNALKVCGKNIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHW 242 LNALK+ K ++D+K+V+ GIGAAGI+ +K+LL AG N+IGVDR GA++R Y +W Sbjct: 250 LNALKIVDKKLEDIKVVLTGIGAAGIACTKILLAAGVRNIIGVDRHGAIHRDETYENPYW 309 Query: 243 NEYARMTNPCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEID 302 EYA++TNP G LSDVI GADVFIGVSAP +LK+EDV MA+DPIVFAMANP PEID Sbjct: 310 QEYAQITNPDNLKGSLSDVIAGADVFIGVSAPGILKVEDVKKMARDPIVFAMANPIPEID 369 Query: 303 PAIAAPHVRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVID 362 P +A P+VRVMATGRSDYPNQINNVLCFPGIFRGALDC A +IN+EMKLAAA+AI+SV+ Sbjct: 370 PELAEPYVRVMATGRSDYPNQINNVLCFPGIFRGALDCRAREINEEMKLAAAKAIASVVT 429 Query: 363 AADLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARK 400 +L+ETYIIP F+ + +G ARK Sbjct: 430 EDELNETYIIPSVFNSKVVERVRQAVIEAAYRTGVARK 467 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,231,806,707 Number of extensions: 89871399 Number of successful extensions: 239659 Number of sequences better than 1.0e-05: 2425 Number of HSP's gapped: 234847 Number of HSP's successfully gapped: 2432 Length of query: 408 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 270 Effective length of database: 1,760,596,133 Effective search space: 475360955910 Effective search space used: 475360955910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)