BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0023.p (283 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B5XP17.1 B5XP17_KLEP3 Putative pyrroline-5-carboxylate reductase 313 1e-83 Q5NQR9.1 Q5NQR9_ZYMMO Pyrroline-5-carboxylate reductase EC=1.5.1.2 299 2e-79 Q9FDK7.1 Q9FDK7_ZYMMO Pyrroline-5-carboxylate reductase 298 5e-79 B2G516.1 B2G516_LACRJ Pyrroline-5-carboxylate reductase 229 2e-58 A5VHI5.1 A5VHI5_LACRD Pyrroline-5-carboxylate reductase EC=1.5.1.2 229 2e-58 >B5XP17.1 B5XP17_KLEP3 Putative pyrroline-5-carboxylate reductase Length = 262 Score = 313 bits (801), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 160/268 (59%), Positives = 196/268 (73%), Gaps = 8/268 (2%) Query: 16 RIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAYGVDTGRSQETYLAE 75 ++HFIG GQM EAIIRA +N T+ A IS+ DI+ AR++ L + Y + S + L E Sbjct: 3 KVHFIGAGQMTEAIIRASLSNGTLRADAISLEDIDSARIEALHSRYRL----SGDGGLRE 58 Query: 76 AELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRVIPNT 135 A L+VL +RPQD L + + +VS++AG+TLEKLA +FG PI R IPNT Sbjct: 59 ASLLVLGIRPQDDLAAVASRVRAQLNGSTTVVSLIAGVTLEKLASLFGETTPIARTIPNT 118 Query: 136 LTDTGYGYSGVVLNANASREQVDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIYYFIES 195 LTDTG+GYSGV LNA+AS +++ FL GFGKV Y+ E +D+FTG+GVAGPNYIYYFIES Sbjct: 119 LTDTGFGYSGVTLNAHASAAEIEPFLRGFGKVLYLPERLIDVFTGFGVAGPNYIYYFIES 178 Query: 196 LVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVGMHGLY 255 L DAGVLAG+ R QA V LEN+ GAV MLRQ+ HPRQLLDINNSP GVG+H LY Sbjct: 179 LTDAGVLAGL----SRPQATEVVLENLLGAVEMLRQSQKHPRQLLDINNSPAGVGIHALY 234 Query: 256 ELNNSDFAAGLQRSVMAAVKRTTELGAK 283 ELNNSDFAAGLQRSV+AAV+RT ELG + Sbjct: 235 ELNNSDFAAGLQRSVLAAVRRTRELGQR 262 >Q5NQR9.1 Q5NQR9_ZYMMO Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 271 Score = 299 bits (766), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 149/272 (54%), Positives = 195/272 (71%), Gaps = 9/272 (3%) Query: 16 RIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAYGVDT----GRSQET 71 IHFIG GQM+EA+IRA+ ++T +A I+ DI R + L + YG+ T ++ ET Sbjct: 3 HIHFIGSGQMSEAMIRAIIKHKTASASDITAYDIRPEREKELHDNYGITTFAKPKKNIET 62 Query: 72 YLAEAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRV 131 L +A+++V+ VRPQD L + I + A A+IVSIVAG+TL + + G R I R+ Sbjct: 63 -LQKADIVVIGVRPQDDLKAVTADIAQNAKEKALIVSIVAGVTLAQFEDLLGKERAIARI 121 Query: 132 IPNTLTDTGYGYSGVVLNANASREQVDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIYY 191 IPNTLTDTG GYSGVV+N A + ++F+ GFGK ++EE+ +DIFTG+GV GPNY+YY Sbjct: 122 IPNTLTDTGLGYSGVVINNQADKALTEKFVSGFGKCLFLEESLIDIFTGFGVCGPNYVYY 181 Query: 192 FIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVGM 251 FIE+LVDAGVLAG+ PR+ AW +EN+ G+V ML +G HPRQLLDINNSP GVGM Sbjct: 182 FIEALVDAGVLAGL----PRDIAWETTMENLVGSVKMLEISGKHPRQLLDINNSPAGVGM 237 Query: 252 HGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 + L+ LN SDF AGLQ+SV+AAVKRTTELG K Sbjct: 238 NSLFALNQSDFTAGLQKSVLAAVKRTTELGQK 269 >Q9FDK7.1 Q9FDK7_ZYMMO Pyrroline-5-carboxylate reductase Length = 271 Score = 298 bits (762), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 149/272 (54%), Positives = 194/272 (71%), Gaps = 9/272 (3%) Query: 16 RIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAYGVDT----GRSQET 71 IHFIG GQM+EA+IRA+ ++T +A I+ DI R + L + YG+ T ++ ET Sbjct: 3 HIHFIGSGQMSEAMIRAIIKHKTASASDITAYDIRPEREKELHDNYGITTFAKPKKNIET 62 Query: 72 YLAEAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRV 131 L +A+++V+ VRPQD L + I + A A+IVSIVAG+TL + + G R I R+ Sbjct: 63 -LQKADIVVIGVRPQDDLKAVTADIAQNAKEKALIVSIVAGVTLAQFEDLLGKERAIARI 121 Query: 132 IPNTLTDTGYGYSGVVLNANASREQVDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIYY 191 IPNTLTDTG GYSGVV+N A + ++F+ GFGK ++EE+ +DIFTG+GV GPNY+YY Sbjct: 122 IPNTLTDTGLGYSGVVINNQADKALTEKFVSGFGKCLFLEESLIDIFTGFGVCGPNYVYY 181 Query: 192 FIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVGM 251 FIE+LVDAGVLAG+ PR+ AW +EN+ G+V ML +G HPRQLLDINNSP GVGM Sbjct: 182 FIEALVDAGVLAGL----PRDIAWETTMENLVGSVKMLEISGKHPRQLLDINNSPAGVGM 237 Query: 252 HGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 + L+ LN SDF AGLQ SV+AAVKRTTELG K Sbjct: 238 NSLFALNQSDFTAGLQNSVLAAVKRTTELGQK 269 >B2G516.1 B2G516_LACRJ Pyrroline-5-carboxylate reductase Length = 270 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 123/272 (45%), Positives = 177/272 (65%), Gaps = 13/272 (4%) Query: 16 RIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAYGVDTGRSQE-TYLA 74 +I+ GGGQM EA++RA + I +TDI+ R+ LT+ Y + + + T + Sbjct: 4 KIYSFGGGQMVEAMLRAALKKKVITPETTGITDISSDRVNYLTDTYHIQASQQPDWTAVE 63 Query: 75 EAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAG---VFGAGRPIVRV 131 A+L++L VRPQD + + + V+ SI+AG+T+ +L+ +F I R+ Sbjct: 64 NADLVILGVRPQDNWQEIMKELVAHHYQKDVL-SIIAGVTVAQLSSQEDLFA----ITRI 118 Query: 132 IPNTLTDTGYGYSGVVLNANASREQVDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIYY 191 IPNTLTD GYSGVV N A++ +++FL FGKV Y++E+ LD+FTGYGVAGPNYIY Sbjct: 119 IPNTLTDVAMGYSGVVKNNRANQTVIEDFLNSFGKVDYIDESLLDVFTGYGVAGPNYIYN 178 Query: 192 FIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVGM 251 F+ S +AGVLAG+ PR+QA +ALEN+ A + Q+G HP +LLDINNS GGVG+ Sbjct: 179 FLISFTNAGVLAGM----PRQQANKLALENLRAAAAFVEQSGKHPAELLDINNSAGGVGI 234 Query: 252 HGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 +EL+ S F+AG++ +V+AAVKRT ELG + Sbjct: 235 TAQHELDKSTFSAGIENAVLAAVKRTKELGKQ 266 >A5VHI5.1 A5VHI5_LACRD Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 270 Score = 229 bits (584), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 123/272 (45%), Positives = 177/272 (65%), Gaps = 13/272 (4%) Query: 16 RIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAYGVDTGRSQE-TYLA 74 +I+ GGGQM EA++RA + I +TDI+ R+ LT+ Y + + + T + Sbjct: 4 KIYSFGGGQMVEAMLRAALKKKVITPETTGITDISSDRVNYLTDTYHIQASQQPDWTAVE 63 Query: 75 EAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAG---VFGAGRPIVRV 131 A+L++L VRPQD + + + V+ SI+AG+T+ +L+ +F I R+ Sbjct: 64 NADLVILGVRPQDNWQEIMKELVAHHYQKDVL-SIIAGVTVAQLSSQEDLFA----ITRI 118 Query: 132 IPNTLTDTGYGYSGVVLNANASREQVDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIYY 191 IPNTLTD GYSGVV N A++ +++FL FGKV Y++E+ LD+FTGYGVAGPNYIY Sbjct: 119 IPNTLTDVAMGYSGVVKNNRANQTVIEDFLNSFGKVDYIDESLLDVFTGYGVAGPNYIYN 178 Query: 192 FIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVGM 251 F+ S +AGVLAG+ PR+QA +ALEN+ A + Q+G HP +LLDINNS GGVG+ Sbjct: 179 FLISFTNAGVLAGM----PRQQANKLALENLRAAAAFVEQSGKHPAELLDINNSAGGVGI 234 Query: 252 HGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 +EL+ S F+AG++ +V+AAVKRT ELG + Sbjct: 235 TAQHELDKSTFSAGIENAVLAAVKRTKELGKQ 266 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,592,754,222 Number of extensions: 65332856 Number of successful extensions: 168291 Number of sequences better than 1.0e-05: 1859 Number of HSP's gapped: 163402 Number of HSP's successfully gapped: 1860 Length of query: 283 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 149 Effective length of database: 1,798,280,193 Effective search space: 267943748757 Effective search space used: 267943748757 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)