BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0021.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C5EES8.1 C5EES8_9FIRM Predicted protein 212 4e-53 B7GPX6.1 B7GPX6_BIFLI Putative uncharacterized protein 187 9e-46 C5EES0.1 C5EES0_9FIRM Uroporphyrinogen decarboxylase 105 7e-21 B9L086.1 B9L086_THERP Uroporphyrinogen decarboxylase 103 3e-20 A7NH05.1 A7NH05_ROSCS Uroporphyrinogen decarboxylase (URO-D) 102 7e-20 >C5EES8.1 C5EES8_9FIRM Predicted protein Length = 343 Score = 212 bits (540), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 121/336 (36%), Positives = 178/336 (52%), Gaps = 14/336 (4%) Query: 4 WSRQERFQAILSGERADRPIVSGWRHFIDKEQNADDL-AGTTIAFTRQYDWDWVKINPRA 62 W+++ER A+L+GE ADRP VS WRHFI+KE + L A T + + R YDWD+VK+ PRA Sbjct: 2 WTKKERVLAVLNGELADRPPVSAWRHFIEKEHSGVGLFAETMLDWHRTYDWDYVKLQPRA 61 Query: 63 TYLAEAWGNEYDFGHYQTVFPRQLTTVVPAAARLWQLEAKKATQSPPLLEQLEAVAKIRR 122 +Y EAWG E+DF +Y+ V P+ + V +A L ++ + P EQ+EAV I+ Sbjct: 62 SYYEEAWGGEFDFENYEGVLPKCIKGPVGSAEDLDKITVLPGNKGP-FAEQIEAVKAIQE 120 Query: 123 GLPD-TPLIQTVFSPLTVLLFIVGRSAYVYPT--VFGIEQPVTLESLFQEXXXXXXXXXX 179 G+ D P+ QT+ P ++L + V P + + +L E Sbjct: 121 GVADGAPIFQTMMCPTSILQKLCA----VNPIGRYRAASRDDLMVTLMHEQPELVHRTLK 176 Query: 180 XXXLTMADYVQELKRAGSDGLFYAVTGTAHPGMFDEASFNEFSRPYDSIVLDAAGYGKNV 239 TMADY ++L G G+FY TG + + + EF +PYD ++DA K + Sbjct: 177 NITATMADYSRQLIHNGLFGVFYGATGLSRSTYLTKEEWEEFVKPYDLEMMDALKPCKIM 236 Query: 240 LHTCGAYAHPERFNDYPVDGISWDTKAEGNPGLEAA-----VTATKVGGVDHALFXXXXX 294 +H CG +PE F YP+D + W A GNP L++A T T +GG D LF Sbjct: 237 VHACGLDVNPEYFAHYPIDILHWPESATGNPALDSAPRWLDRTITPMGGCDERLFGQHKA 296 Query: 295 XXXXXXXSEALRQMKDQPFILAPNCAIPLSVTDEAL 330 L++MKD PF+LAP+C++ L+ D+ L Sbjct: 297 DEIGAMTRNTLKRMKDIPFMLAPDCSLALNTYDDEL 332 >B7GPX6.1 B7GPX6_BIFLI Putative uncharacterized protein Length = 347 Score = 187 bits (476), Expect = 9e-46, Method: Compositional matrix adjust. Identities = 125/344 (36%), Positives = 171/344 (49%), Gaps = 18/344 (5%) Query: 5 SRQERFQAILSGERADRPIVSGWRHFIDKEQNADDLAGTTIAFTRQYDWDWVKINPRATY 64 +R+ F AI++ + +VS W+H + E A++ A TI F ++DW+WVKINPRA Y Sbjct: 3 NRRADFHAIVNHQSRTPYLVSAWQHLVGHEYGAEEFAKATIDFVNRWDWEWVKINPRAIY 62 Query: 65 LAEAWGNEYDFGHYQT-VFPRQLTTVVPAAARLWQLEAKKATQSPPLLEQLEAVAKIRRG 123 AEAWG+ YD Y V P+++ + + ++ + E L A A IR G Sbjct: 63 YAEAWGSRYDRNDYAGFVLPKKIGAAITEPDDVRGVKELDVRDNEYFAEALAAAALIRHG 122 Query: 124 LPDTPLIQTVFSPLTVLLFIVGRSAYVYPTVFGIEQPVTLESLFQEXXXXXXXXXXXXXL 183 L D ++QTVFSPL+VLL I +YP + +E L Sbjct: 123 LEDRAVLQTVFSPLSVLLQIADLP--LYPGDDYATATLRVEDLIFNQPGVAKLALANIAR 180 Query: 184 TMADYVQELKR------AGSDGLFYAVTGTAHPGMFDEASFNEFSRPYDSIVLDAAGYGK 237 T+A Y + L R AG DG+FYA+TGT G FD + EFS PYD IV+DA Sbjct: 181 TLASYARALVRPESEGGAGLDGVFYALTGTVSEGYFDAQRYLEFSEPYDRIVIDAVHRAN 240 Query: 238 N----VLHTCGAYAHPERFNDYPVDGISWDTKAEGNPGLEAAVTATKVGGVDHALFX--- 290 +LHTC A +HP F+ VD + WD GNP ++A +TA V G + LF Sbjct: 241 PDAVVLLHTCRAESHPNWFDVKGVDLLQWDQYQPGNPQVDADLTAVPVAGAPYDLFAPDG 300 Query: 291 XXXXXXXXXXXSEALRQMKDQPFILAPNCAIPLSVTDEALVQLK 334 + ALR K PF+LAP+C +P DEAL L Sbjct: 301 DVEQVRRELAKTSALRTGK--PFLLAPSCTVPTPAGDEALQALS 342 >C5EES0.1 C5EES0_9FIRM Uroporphyrinogen decarboxylase Length = 332 Score = 105 bits (262), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 86/351 (24%), Positives = 156/351 (44%), Gaps = 41/351 (11%) Query: 5 SRQERFQAILSGERADRPIVSGWRHFIDKEQNADDLAGTTIAFTRQYDWDWVKINPRATY 64 ++ ER +A+L G + D P ++ W+HF +++ +L I F ++ WD+VK+ + + Sbjct: 2 TKTERIKALLEGRKLDTPSMNLWKHFPPYDEDPKELVKKIIQFQDRFQWDFVKVTYQGLF 61 Query: 65 LAEAWGNEYDFGHYQTVFPRQLTT--VVPAAARLWQLEAKKATQSPPLLEQLEAVAKIRR 122 + WG+ + V+P ++ VV + + K P + A + + Sbjct: 62 SIQDWGSTIKWPVRDNVWPETCSSVGVVTDCSIKHDEDWTKLNVLPMDQGSMAATIEAAK 121 Query: 123 GLPD-----TPLIQTVFSPLTVLLFIVGRSAYVY----PTVFGIEQPVTLESLFQEXXXX 173 G+ D P++ T+F+PLT + G V+ P F LE + Q Sbjct: 122 GVVDHYKGEVPVVITIFNPLTTAAKMSGDKMLVHMRRSPEAFH----KGLEIICQ----- 172 Query: 174 XXXXXXXXXLTMADYVQELKRAGSDGLFYAVTGTAHPGMFDE---ASFNEFSRPYDSIVL 230 T + V+E+ + G+DG+F+A + G +D+ A + EF RPYD VL Sbjct: 173 ----------TTINLVKEMSKLGADGIFFA----SQLGTYDKMSVAEYEEFGRPYDLRVL 218 Query: 231 DAA-GYGKNVLHTCGAYAHPERFNDYPVDGISWDTKAEGNPGLEAAVTATK--VGGVDH- 286 +AA N++H G E + YPV ++W + + + K +GGV+ Sbjct: 219 EAAQDMWFNIMHMHGNAPMFELLSQYPVQALNWHDQLVDISLADGRRMSDKLLIGGVEEL 278 Query: 287 ALFXXXXXXXXXXXXSEALRQMKDQPFILAPNCAIPLSVTDEALVQLKKSV 337 + +L Q+ D ILAP C +PL V ++ L + ++++ Sbjct: 279 KVLAEADDEQLKAHLKSSLEQVPDGRLILAPGCCVPLYVPEDRLARAQEAL 329 >B9L086.1 B9L086_THERP Uroporphyrinogen decarboxylase Length = 335 Score = 103 bits (256), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 97/335 (28%), Positives = 144/335 (42%), Gaps = 43/335 (12%) Query: 8 ERFQAILSGERADRPIVSGWRHFIDKEQNADDLAGTTIAFTRQYDWDWVKINPRATYLAE 67 ER +A ++GE D P VS WRHF +++Q+A LA T+A+ R++ +D+VK+ P Y Sbjct: 9 ERIEAAIAGESVDHPPVSLWRHFPEEDQDAATLARVTLAWQRRFAFDFVKLMPPGDYPVI 68 Query: 68 AWGNEYDF----------GHYQTVFPRQLTTVVPAAARLWQLEAKKATQSPPLLEQLEAV 117 AWG ++ Y P T+ P L P EA Sbjct: 69 AWGGASEYRGSPSGTRTVTRYPVEQPADWPTIRPLPVDLG-----------PFRTVNEAA 117 Query: 118 AKIRRGLPD-TPLIQTVFSPLTVLLFI-VGRSAYVYPTVFGIEQPVTLESLFQEXXXXXX 175 + R L P++QTVFSPLTV + GR + +E P +E Sbjct: 118 RLVARELGGAVPVLQTVFSPLTVAYKLSAGRVLHDL-----VEHPALVEEALAAITE--- 169 Query: 176 XXXXXXXLTMADYVQELKRAGSDGLFYAVTGTAHPGMFDEASFNEFSRPYDSIVLD-AAG 234 TM V AG+DGLF+A T A + D A++ + RP D VL+ AAG Sbjct: 170 --------TMRALVHAALAAGADGLFFA-TQCATADLTDPATYARWGRPGDLAVLEAAAG 220 Query: 235 YGKNVLHTCGAYAHPERFNDYPVDGISWDTKAEGNPGLEAAVTATKV--GGVDHALFXXX 292 +LH G H + DYPV ++W + G E + + GG+D Sbjct: 221 APFLLLHIHGPAPHFDLLADYPVHVLNWHDRRAGPSLAEGEQRSGRCVAGGLDETTIATL 280 Query: 293 XXXXXXXXXSEALRQMKDQPFILAPNCAIPLSVTD 327 +A+ Q+K + ++ P C IP++ D Sbjct: 281 APKAVAAQARDAIAQLKGRHLMVTPGCVIPIATPD 315 >A7NH05.1 A7NH05_ROSCS Uroporphyrinogen decarboxylase (URO-D) Length = 333 Score = 102 bits (253), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 90/334 (26%), Positives = 147/334 (44%), Gaps = 33/334 (9%) Query: 3 TWSRQERFQAILSGERADRPIVSGWRHFIDKEQNADDLAGTTIAFTRQYDWDWVKINPRA 62 + +R+ER A + GE DRP V+ WRHF +Q+ + LA + AF QYDWD+VK P + Sbjct: 2 SMTRRERLAAAIRGEPVDRPPVALWRHFPVDDQDPEQLALSVAAFQSQYDWDFVKFTPSS 61 Query: 63 TYLAEAWGNEYDF-GHYQTVFPRQLTTV-VPAAARLWQLEAKKATQSPPLLEQLEAVAKI 120 ++ E WG + GH + V VPA W+ ++ L L AV + Sbjct: 62 SFCVENWGCRVVYRGHSEGTSDYVARPVSVPAD---WRRITPLDPRAGALGAHLVAVRRA 118 Query: 121 RRGL-PDTPLIQTVFSPLTVLLFIVGRSA-YVYPTVFGIEQPVTLESLFQEXXXXXXXXX 178 R + PD PL+ TVFSP++ ++G V+ + LE++ + Sbjct: 119 RALIDPDVPLLATVFSPISQAKNLIGGGMDIVHLRRHRSDLLDALEAITE---------- 168 Query: 179 XXXXLTMADYVQELKRAGSDGLFYAVTGTAHPGMFDEASFNEFSRPYDSIVLD-----AA 233 T +V+ + G+DG+FYA+ + EA + E RP D +L+ +A Sbjct: 169 -----TTIRFVEAVLETGADGIFYAMQ-RCTADVISEAEYREVCRPLDMRILEAAHAASA 222 Query: 234 GYGKN---VLHTCGAYAHPERFNDYPVDGISWDTKAEGNPGLEAA--VTATKVGGVDHAL 288 +GK +LH G +++ + +YP ++W + G E A VGG+ Sbjct: 223 AHGKPPFILLHLHGMHSYFDIAAEYPAQALNWHDRDTGPDLAEGARRFPGMVVGGLSQRD 282 Query: 289 FXXXXXXXXXXXXSEALRQMKDQPFILAPNCAIP 322 +A+ M + L+ C +P Sbjct: 283 IVEGSPTAVQSLARQAIAAMGGRRMCLSTGCVMP 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,952,981,859 Number of extensions: 75062489 Number of successful extensions: 201961 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 201938 Number of HSP's successfully gapped: 13 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)