BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0016.p (404 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z892.1 C0Z892_BREBN Probable methionine gamma-lyase EC=4.4.1.11 546 e-153 B2A179.1 B2A179_NATTJ Cystathionine gamma-synthase EC=4.4.1.11 397 e-109 B2A843.1 B2A843_NATTJ Methionine gamma-lyase EC=4.4.1.11 397 e-108 C1MDK3.1 C1MDK3_9ENTR Methionine gamma-lyase 390 e-107 C2AY55.1 C2AY55_9ENTR Putative uncharacterized protein 390 e-106 >C0Z892.1 C0Z892_BREBN Probable methionine gamma-lyase EC=4.4.1.11 Length = 402 Score = 546 bits (1406), Expect = e-153, Method: Compositional matrix adjust. Identities = 270/390 (69%), Positives = 312/390 (80%) Query: 4 EWKMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPT 63 +WKMDT IIH +Q+P TGA+ +V+PAVAYAFPD YYGRYGNPT Sbjct: 5 KWKMDTSIIHTAQTPCQKTGAVVSAVVPAVAYAFPDADSAAACVAGEREGTYYGRYGNPT 64 Query: 64 SRTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAP 123 TLE+KIAALE GE ALGVSSGMAAIS ALL FLK GDHV+ T+DVYGG+Y FLT++AP Sbjct: 65 ISTLEQKIAALENGEAALGVSSGMAAISGALLAFLKQGDHVVCTRDVYGGSYKFLTTMAP 124 Query: 124 RFGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKLPVIVD 183 R+GI DFVDCTD A+ A PNTK +YIETPSNP LT+LDI ++ H + VIVD Sbjct: 125 RYGIATDFVDCTDLEAVESAFLPNTKVLYIETPSNPCLTVLDISALSLLAHAHGIVVIVD 184 Query: 184 NTFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGDLGQNL 243 NTF++P LQ+PLELGADVVVHSATKY+NGHGDV+AGF+VG ++ I+FMRK +MGDLGQNL Sbjct: 185 NTFLTPYLQRPLELGADVVVHSATKYLNGHGDVIAGFIVGKQEHIQFMRKNVMGDLGQNL 244 Query: 244 NAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLALAQM 303 NAWD+FLILRGMKT+ LRV +H +NAQ VAEFL HP+I VYYPGL SHPQH+LA QM Sbjct: 245 NAWDAFLILRGMKTLGLRVRQHGQNAQAVAEFLAKHPAISHVYYPGLPSHPQHELAKRQM 304 Query: 304 KGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKERVQF 363 GMGGIVSFEVKGG K IN+L LAMISFSLGDPETLVQHPASMTH+SIP ER++F Sbjct: 305 AGMGGIVSFEVKGGYEAAKSFINALHLAMISFSLGDPETLVQHPASMTHSSIPASERIKF 364 Query: 364 GITDGLIRVSVGLEDVDDIIADFEQALAAL 393 ITDGLIR+S GLEDV+DIIAD EQALA+L Sbjct: 365 NITDGLIRLSTGLEDVEDIIADLEQALASL 394 >B2A179.1 B2A179_NATTJ Cystathionine gamma-synthase EC=4.4.1.11 Length = 392 Score = 397 bits (1020), Expect = e-109, Method: Compositional matrix adjust. Identities = 183/386 (47%), Positives = 268/386 (69%), Gaps = 1/386 (0%) Query: 6 KMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTSR 65 K+ TRI+H + GA+ + + + F + Y R GNPT + Sbjct: 5 KLTTRIVHADEGQKNEAGALRTPIYQSSTFVFDNVEQGAARFAGEEAGYIYTRLGNPTQK 64 Query: 66 TLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPRF 125 LEK++A LEGG+DA+ + SGMAA+S ++ L GDH++ + +YG T++ L P + Sbjct: 65 NLEKQMANLEGGDDAIALGSGMAAVSAVVMNLLNCGDHMVASDTLYGCTHSLFDELLPNW 124 Query: 126 GIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACK-LHKLPVIVDN 184 GIE FVD ++ + AIK NTK +Y ETP+NP ++I D++++ + K + +VDN Sbjct: 125 GIEVTFVDTSEPENIRAAIKDNTKVIYTETPANPTISITDLEEVVKIAKEKDDITTVVDN 184 Query: 185 TFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGDLGQNLN 244 TFM+P LQ+PLE+G D+V+HSATKY+ GHGD +AG VVG +++I ++ + DLG + Sbjct: 185 TFMTPYLQRPLEMGIDIVIHSATKYLGGHGDTVAGIVVGFEELIGDIKMTTVKDLGGIIA 244 Query: 245 AWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLALAQMK 304 +D+FL+LRG+KT+A+RV++ C NA VA+FLE HP++ERVYYPGLESHPQH+LA QM+ Sbjct: 245 PFDAFLLLRGIKTLAVRVDKICENAMKVAKFLEEHPAVERVYYPGLESHPQHELAKKQME 304 Query: 305 GMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKERVQFG 364 GGI++FE+KGG GK L+N+++L ++ SLGD +TL+QHPASMTH+ +PQ+ER + G Sbjct: 305 DFGGIIAFELKGGFEAGKNLLNNVELCTLAVSLGDVDTLIQHPASMTHSVVPQEEREKAG 364 Query: 365 ITDGLIRVSVGLEDVDDIIADFEQAL 390 ITDG++RVSVG+EDVDDII D +Q + Sbjct: 365 ITDGMVRVSVGIEDVDDIIEDLKQGM 390 >B2A843.1 B2A843_NATTJ Methionine gamma-lyase EC=4.4.1.11 Length = 390 Score = 397 bits (1020), Expect = e-108, Method: Compositional matrix adjust. Identities = 190/385 (49%), Positives = 264/385 (68%) Query: 6 KMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTSR 65 +TRI+H + GA+ + + + F Y R GNPT Sbjct: 5 NFETRILHADEGHKNDAGALRVPIYQSSTFVFDSVEQGASRFTGEEDGYIYTRLGNPTQS 64 Query: 66 TLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPRF 125 +LEK +A LEGGEDAL VSSGMAAIS A++ L GDH++V+ +YG T++F + P + Sbjct: 65 SLEKLMANLEGGEDALAVSSGMAAISSAIMSLLSCGDHMIVSNPLYGCTHSFCVDILPEW 124 Query: 126 GIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKLPVIVDNT 185 GIE FVD TD + +AIK NTK +Y ETP+NP L ++D++++ + K L I DNT Sbjct: 125 GIEVTFVDMTDPDNIYKAIKSNTKVIYTETPANPTLYLIDLERVAKIAKEKNLTTITDNT 184 Query: 186 FMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVIRFMRKRLMGDLGQNLNA 245 FMSP LQ+PLELG DVVVHSATKY+ GHGD +AG +VG K +I + + + G + Sbjct: 185 FMSPYLQRPLELGIDVVVHSATKYLCGHGDTVAGMIVGPKKIIDNINGTTIKNFGGIIGP 244 Query: 246 WDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLALAQMKG 305 +D+FLILRG+KT+ LR+++ C NA+ +AEFL+ HP+IE+VYYPGLESHPQH+LA QM+ Sbjct: 245 FDAFLILRGVKTLTLRMDKICENAKKIAEFLDNHPAIEKVYYPGLESHPQHELAKRQMRN 304 Query: 306 MGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKERVQFGI 365 GGIV+FE+ G GKKL+N++++ ++ SLGD +TL+QHPASMTH +P++ER + GI Sbjct: 305 FGGIVTFELADGYEAGKKLLNNVEVCTLATSLGDVDTLIQHPASMTHYVVPKEEREKNGI 364 Query: 366 TDGLIRVSVGLEDVDDIIADFEQAL 390 TDGL+R+SVG+E+V+DII D +Q L Sbjct: 365 TDGLVRLSVGIENVNDIIEDLKQGL 389 >C1MDK3.1 C1MDK3_9ENTR Methionine gamma-lyase Length = 398 Score = 390 bits (1003), Expect = e-107, Method: Compositional matrix adjust. Identities = 190/390 (48%), Positives = 256/390 (65%), Gaps = 7/390 (1%) Query: 5 WKMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTS 64 + +T+I+H Q PDP TGA+S + + F Y R GNPT+ Sbjct: 7 YGFNTQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARFALEESGYIYTRLGNPTT 66 Query: 65 RTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPR 124 LEKK+A LE GE L +SG++AI+ LL + GDH++ +YG T+ FL+ P+ Sbjct: 67 DALEKKLAVLERGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYGCTHAFLSHSMPK 126 Query: 125 FGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKLPVIVDN 184 FGI FVD + A++P TK VYIETP+NP L+++DI+ + ++VDN Sbjct: 127 FGINVSFVDAAKPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDN 186 Query: 185 TFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVI---RFM-RKRLMGDLG 240 TFMSP Q+PL+LGAD+VVHS TKYINGHGDV+ G +VG ++ I RF+ K + G Sbjct: 187 TFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGKQEFIDQARFVGLKDITGGCM 246 Query: 241 QNLNAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLAL 300 NAW L LRG+KT+ +R+ERHC NA +A FLE HPSI RVYYPGL SHPQ++L Sbjct: 247 SPFNAW---LTLRGVKTLGIRMERHCENALKIARFLEGHPSITRVYYPGLTSHPQYELGQ 303 Query: 301 AQMKGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKER 360 QM GGI+SFE+ GGL G+++INS++L +++ SLGD ETL+QHPASMTH+ + +ER Sbjct: 304 RQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEER 363 Query: 361 VQFGITDGLIRVSVGLEDVDDIIADFEQAL 390 ++ GITDGLIR+SVGLED +DII D E A+ Sbjct: 364 LKAGITDGLIRLSVGLEDPEDIINDLEHAI 393 >C2AY55.1 C2AY55_9ENTR Putative uncharacterized protein Length = 398 Score = 390 bits (1002), Expect = e-106, Method: Compositional matrix adjust. Identities = 189/390 (48%), Positives = 256/390 (65%), Gaps = 7/390 (1%) Query: 5 WKMDTRIIHESQSPDPLTGAISQSVIPAVAYAFPDXXXXXXXXXXXXXXXYYGRYGNPTS 64 + +T+I+H Q PDP TGA+S + + F Y R GNPT+ Sbjct: 7 YGFNTQIVHAGQQPDPSTGALSTPIFQTSTFVFDSAEQGAARFALEESGYIYTRLGNPTT 66 Query: 65 RTLEKKIAALEGGEDALGVSSGMAAISIALLGFLKHGDHVLVTKDVYGGTYNFLTSLAPR 124 LEKK+A LE GE L +SG++AI+ LL + GDH++ +YG T+ FL+ P+ Sbjct: 67 DALEKKLAVLEKGEAGLATASGISAITTTLLTLCQQGDHIVSASAIYGCTHAFLSHSMPK 126 Query: 125 FGIECDFVDCTDTAAMLQAIKPNTKAVYIETPSNPKLTILDIKQITQACKLHKLPVIVDN 184 FGI FVD + + A++P TK VYIETP+NP L+++DI+ + ++VDN Sbjct: 127 FGINVSFVDAANPEEIRAAMRPETKVVYIETPANPTLSLVDIETVAGIAHQQGALLVVDN 186 Query: 185 TFMSPCLQKPLELGADVVVHSATKYINGHGDVLAGFVVGSKDVI---RFM-RKRLMGDLG 240 TFMSP Q+PL+LGAD+VVHS TKYINGHGDV+ G +VG ++ I RF+ K + G Sbjct: 187 TFMSPYCQQPLQLGADIVVHSVTKYINGHGDVIGGIIVGKQEFIDQARFVGLKDITGGCM 246 Query: 241 QNLNAWDSFLILRGMKTMALRVERHCRNAQTVAEFLEAHPSIERVYYPGLESHPQHKLAL 300 NAW L LRG+KT+ +R+ERHC NA +A FLE HPSI VYYPGL SHPQ++L Sbjct: 247 SPFNAW---LTLRGVKTLGIRIERHCENALKIARFLEGHPSITHVYYPGLASHPQYELGQ 303 Query: 301 AQMKGMGGIVSFEVKGGLTEGKKLINSLKLAMISFSLGDPETLVQHPASMTHASIPQKER 360 QM GGI+SFE+ GGL G+++INS++L +++ SLGD ETL+QHPASMTH+ + +ER Sbjct: 304 RQMSLPGGIISFEIAGGLEAGRRMINSVELCLLAVSLGDTETLIQHPASMTHSPVAPEER 363 Query: 361 VQFGITDGLIRVSVGLEDVDDIIADFEQAL 390 ++ GITDGLIR+SVGLED +DII D E A+ Sbjct: 364 LKAGITDGLIRLSVGLEDPEDIINDLEHAI 393 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,295,365,460 Number of extensions: 90404457 Number of successful extensions: 236089 Number of sequences better than 1.0e-05: 4576 Number of HSP's gapped: 225653 Number of HSP's successfully gapped: 5676 Length of query: 404 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 266 Effective length of database: 1,760,596,133 Effective search space: 468318571378 Effective search space used: 468318571378 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)