BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0013.p (307 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0Z8V5.1 C0Z8V5_BREBN Putative oxidoreductase 400 e-110 B3KC49.1 B3KC49_9BACI Alcohol dehydrogenase zinc-binding domain ... 367 e-100 B3KHM3.1 B3KHM3_9BACI Alcohol dehydrogenase zinc-binding domain ... 362 2e-98 C2P992.1 C2P992_BACCE Alcohol dehydrogenase, zinc containing 360 7e-98 C3BWK0.1 C3BWK0_BACTU Alcohol dehydrogenase, zinc containing 358 2e-97 >C0Z8V5.1 C0Z8V5_BREBN Putative oxidoreductase Length = 310 Score = 400 bits (1028), Expect = e-110, Method: Compositional matrix adjust. Identities = 192/302 (63%), Positives = 232/302 (76%) Query: 1 MKAMVCSKYGSPENLHLIEMEKPVPKRKDLLIKVHASTVTAGDIRVRTFKSPVLLWLPMR 60 M+A++C+KYGSP+ L L E+E P+PK ++LIKVHA+TVT+GD R+R FKSP LLW+PMR Sbjct: 1 MRAVICTKYGSPDVLELREVEHPIPKDNEILIKVHATTVTSGDCRIRAFKSPFLLWIPMR 60 Query: 61 LVLGLSKPRKPXXXXXXXXXXXXXXXXXTRFKPGDPVYALTGMRFGGHAEYACLSEDAMV 120 +VLGL+KPR P RFK GD VYALTGMR G HAEY CL E+A+V Sbjct: 61 IVLGLTKPRNPILGVELSGEVEAIGKHVKRFKKGDHVYALTGMRCGAHAEYICLQENAIV 120 Query: 121 SIKPANTTFEEAASTLFGGTTALYFLRKANIQKGQKVLIYGASGTVGTFALQLARHFGAE 180 +KP N T EEAA+ FGGTTAL+F RK +QKGQKVLIYGASG+VGT A+QLA++ GAE Sbjct: 121 GLKPTNVTHEEAAAIPFGGTTALHFFRKGKLQKGQKVLIYGASGSVGTAAVQLAKYLGAE 180 Query: 181 VDGVCSTANLEWVKSLGASQVIDYTAEDCTQRGQQYDIIFDAVGKLPKAGCKKALKPNGS 240 V VCS+AN + VKSLGA VIDYT ED T++ ++YD+IFDAVGK K+ C KAL PNG Sbjct: 181 VTAVCSSANFDLVKSLGADTVIDYTKEDFTKKEERYDVIFDAVGKATKSHCVKALVPNGI 240 Query: 241 YVTVDGQGMAKVRLEDLQLLSELMEAGNIKSVIDRYYPLEQLAEAHRYVEQGHKKGSVVI 300 ++TVDGQG+AKVR EDL L ELMEAG IK+VIDR Y LEQ+ EAHRYVE G KKG+VVI Sbjct: 241 FLTVDGQGIAKVRTEDLVFLKELMEAGEIKAVIDRRYSLEQVPEAHRYVETGRKKGNVVI 300 Query: 301 RI 302 R+ Sbjct: 301 RV 302 >B3KC49.1 B3KC49_9BACI Alcohol dehydrogenase zinc-binding domain protein Length = 306 Score = 367 bits (943), Expect = e-100, Method: Compositional matrix adjust. Identities = 177/304 (58%), Positives = 222/304 (73%) Query: 1 MKAMVCSKYGSPENLHLIEMEKPVPKRKDLLIKVHASTVTAGDIRVRTFKSPVLLWLPMR 60 MKAMVC+ YG PE L L E+ KP+PK ++ IK++A+TV +GD RVR F+SP+L +PMR Sbjct: 1 MKAMVCTAYGPPEVLQLQEVAKPIPKDHEVRIKIYATTVNSGDCRVRGFRSPLLYKIPMR 60 Query: 61 LVLGLSKPRKPXXXXXXXXXXXXXXXXXTRFKPGDPVYALTGMRFGGHAEYACLSEDAMV 120 LVLGL KPR+P TRFK GD V A GMRFG +A+Y C+ ED M+ Sbjct: 61 LVLGLRKPRQPILGVELAGEVEAIGQDVTRFKIGDQVLAFRGMRFGTYAQYICMHEDGMI 120 Query: 121 SIKPANTTFEEAASTLFGGTTALYFLRKANIQKGQKVLIYGASGTVGTFALQLARHFGAE 180 +KP + T+++AA+ FGGTTAL+F RKANI++G KVLIYGASG VG A+QLA+H G Sbjct: 121 VMKPEHVTYDDAAAIFFGGTTALHFFRKANIRQGHKVLIYGASGAVGNLAVQLAKHAGTV 180 Query: 181 VDGVCSTANLEWVKSLGASQVIDYTAEDCTQRGQQYDIIFDAVGKLPKAGCKKALKPNGS 240 V GVCST N+E VKSLGA QV+DYT ED ++ G +YDII DAVGKLPK+ CK L GS Sbjct: 181 VTGVCSTVNVELVKSLGADQVVDYTQEDISKSGDRYDIILDAVGKLPKSRCKHLLAAGGS 240 Query: 241 YVTVDGQGMAKVRLEDLQLLSELMEAGNIKSVIDRYYPLEQLAEAHRYVEQGHKKGSVVI 300 YVTV+GQG+AKV +EDL+ L++L+ G IK VIDR Y LEQ+AEAHRY + G K+GSVVI Sbjct: 241 YVTVEGQGVAKVLMEDLRRLTDLLGEGKIKPVIDRRYALEQIAEAHRYADTGRKRGSVVI 300 Query: 301 RIGQ 304 +G Sbjct: 301 TVGH 304 >B3KHM3.1 B3KHM3_9BACI Alcohol dehydrogenase zinc-binding domain protein Length = 308 Score = 362 bits (930), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 170/306 (55%), Positives = 218/306 (71%) Query: 1 MKAMVCSKYGSPENLHLIEMEKPVPKRKDLLIKVHASTVTAGDIRVRTFKSPVLLWLPMR 60 MKAMVC+KYGSP+ L L E+ KPVP ++L+++ A++V A D+R+R+ P+LLW+PMR Sbjct: 1 MKAMVCTKYGSPDVLELTEVNKPVPMANEVLVRIRATSVAAMDVRIRSSDFPLLLWIPMR 60 Query: 61 LVLGLSKPRKPXXXXXXXXXXXXXXXXXTRFKPGDPVYALTGMRFGGHAEYACLSEDAMV 120 +LG KP+K RFK GD + A GM FG +AEY CL ED MV Sbjct: 61 FLLGFRKPKKTILGVELSGIIEDVGKDVKRFKKGDEIMAYRGMNFGTYAEYTCLHEDGMV 120 Query: 121 SIKPANTTFEEAASTLFGGTTALYFLRKANIQKGQKVLIYGASGTVGTFALQLARHFGAE 180 +KP N+T+EEAA+ FGG +ALYFL+K+NIQ GQ+V YGASG VG+ A+QLA+HFG E Sbjct: 121 VMKPINSTYEEAAAFAFGGLSALYFLKKSNIQSGQRVPFYGASGAVGSSAVQLAKHFGTE 180 Query: 181 VDGVCSTANLEWVKSLGASQVIDYTAEDCTQRGQQYDIIFDAVGKLPKAGCKKALKPNGS 240 V GV ST N++ VK+LGA V+DYT E+ R ++YD+IFDAVGK+ KA K L PNG+ Sbjct: 181 VTGVTSTTNIDLVKTLGADLVVDYTTENFVNRNERYDVIFDAVGKISKARSSKVLAPNGT 240 Query: 241 YVTVDGQGMAKVRLEDLQLLSELMEAGNIKSVIDRYYPLEQLAEAHRYVEQGHKKGSVVI 300 YV+VDGQG+AKV +DL L ELME G +KSVIDR YPLEQ+ EAHRY E G KKG+V I Sbjct: 241 YVSVDGQGIAKVSTKDLHFLKELMEKGKLKSVIDRCYPLEQIPEAHRYAETGRKKGNVAI 300 Query: 301 RIGQGR 306 +G+ R Sbjct: 301 TVGECR 306 >C2P992.1 C2P992_BACCE Alcohol dehydrogenase, zinc containing Length = 322 Score = 360 bits (925), Expect = 7e-98, Method: Compositional matrix adjust. Identities = 169/302 (55%), Positives = 225/302 (74%), Gaps = 1/302 (0%) Query: 1 MKAMVCSKYGSPENLHLIEMEKPVPKRKDLLIKVHASTVTAGDIRVRTFKSPVLLWLPMR 60 MKA++C++YG P L L +EKP PK+ ++LIK+HA++V+ GD R+R F SP+L W+PMR Sbjct: 21 MKAIICTQYGPPNVLQLQNIEKPTPKKNEVLIKIHATSVSTGDCRIRGFNSPLLFWIPMR 80 Query: 61 LVLGLSKPRKPXXXXXXXXXXXXXXXXXTRFKPGDPVYALTGMRFGGHAEYACLSEDAMV 120 ++LG KPRKP T+FK GDP++ALT + GG+AEY C+ E ++ Sbjct: 81 IILGFRKPRKPVLGVELSGEIEEIGTDVTQFKKGDPIFALTELNLGGYAEYTCVHESGLI 140 Query: 121 SIKPANTTFEEAASTLFGGTTALYFLRKANIQKGQKVLIYGASGTVGTFALQLARHFGAE 180 ++KP N T+EEAA FGGT+AL+FLRK I+KGQ+VLIYGASG+VGT A+QLA++FGA Sbjct: 141 TLKPTNVTYEEAAVISFGGTSALHFLRKGRIKKGQQVLIYGASGSVGTAAVQLAKYFGAT 200 Query: 181 VDGVCSTANLEWVKSLGASQVIDYTAEDCTQRGQQYDIIFDAVGKLPKAGCKKALKPNGS 240 V VCS +N E V+SLGA +VIDYT ED T+ G++Y+IIFDAVGK K+ C+KAL PNG Sbjct: 201 VTAVCSNSNFELVQSLGADKVIDYTKEDFTKLGERYNIIFDAVGKHKKSLCEKALTPNGK 260 Query: 241 YVTVDGQGMAKVRLEDLQLLSELMEAGNIKSVIDRYYPLEQLAEAHRYVEQGHKKGSVVI 300 YV+V+G MAKV ED+ LL +L EA ++K VIDR Y LE++AEAH YVE+GHKKG+V I Sbjct: 261 YVSVNGM-MAKVSKEDMILLKKLTEAEHLKPVIDRTYRLEEIAEAHIYVEKGHKKGNVSI 319 Query: 301 RI 302 + Sbjct: 320 TL 321 >C3BWK0.1 C3BWK0_BACTU Alcohol dehydrogenase, zinc containing Length = 302 Score = 358 bits (920), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 169/302 (55%), Positives = 223/302 (73%), Gaps = 1/302 (0%) Query: 1 MKAMVCSKYGSPENLHLIEMEKPVPKRKDLLIKVHASTVTAGDIRVRTFKSPVLLWLPMR 60 MKA++C+KYG P L L +EKP+PK+ ++L+K+HA++VT GD R+R F SP+L W+PMR Sbjct: 1 MKAIICTKYGPPNVLQLQNIEKPIPKKNEVLVKIHATSVTTGDCRIRGFNSPLLFWIPMR 60 Query: 61 LVLGLSKPRKPXXXXXXXXXXXXXXXXXTRFKPGDPVYALTGMRFGGHAEYACLSEDAMV 120 ++LG KPRKP T+FK GD ++ALT + GG+AEY C+ E +V Sbjct: 61 IILGFRKPRKPILGVELSGEIEEIGTDVTQFKKGDQIFALTELNVGGYAEYTCVHESGLV 120 Query: 121 SIKPANTTFEEAASTLFGGTTALYFLRKANIQKGQKVLIYGASGTVGTFALQLARHFGAE 180 ++KP N T+EEAA FG T+AL+FLRKA I++GQ+VLIYGASG+VGT A+QLA++FGA Sbjct: 121 ALKPTNVTYEEAAVIPFGATSALHFLRKARIKRGQQVLIYGASGSVGTAAVQLAKYFGAT 180 Query: 181 VDGVCSTANLEWVKSLGASQVIDYTAEDCTQRGQQYDIIFDAVGKLPKAGCKKALKPNGS 240 V VCS +N E V+SLGA +VIDYT ED T++G+ YD+IFDAVGK K+ CK+ L PNG Sbjct: 181 VTAVCSHSNFELVQSLGADKVIDYTKEDFTKQGEYYDVIFDAVGKYKKSLCKQVLTPNGI 240 Query: 241 YVTVDGQGMAKVRLEDLQLLSELMEAGNIKSVIDRYYPLEQLAEAHRYVEQGHKKGSVVI 300 YVTV+G MAKV ED+ LL +L E N+K VIDR Y LE++AEAH YVE+GHKKG+V I Sbjct: 241 YVTVNGM-MAKVSKEDMFLLKKLTETENLKPVIDRTYRLEEIAEAHIYVEKGHKKGNVSI 299 Query: 301 RI 302 + Sbjct: 300 TL 301 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,694,993,886 Number of extensions: 63413706 Number of successful extensions: 179213 Number of sequences better than 1.0e-05: 3857 Number of HSP's gapped: 177488 Number of HSP's successfully gapped: 3880 Length of query: 307 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 172 Effective length of database: 1,788,859,178 Effective search space: 307683778616 Effective search space used: 307683778616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)