BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0009.p (586 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0ZAE8.1 C0ZAE8_BREBN ABC transporter ATP binding/permease protein 276 4e-72 A5UR27.1 A5UR27_ROSS1 ABC transporter related 261 1e-67 Q93GH7.1 Q93GH7_BACSU Putative SpaT 254 2e-65 P33116.1 SPAT_BACSU Subtilin transport ATP-binding protein spaT 253 3e-65 B8HPX2.1 B8HPX2_CYAP4 ABC transporter related 253 5e-65 >C0ZAE8.1 C0ZAE8_BREBN ABC transporter ATP binding/permease protein Length = 594 Score = 276 bits (706), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 173/553 (31%), Positives = 281/553 (50%), Gaps = 29/553 (5%) Query: 45 YKLLIDDLAFVHSASAVTQKLVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQ 104 Y+ L+ LA + S + VT L TVL+VL AH RL+Y++ Sbjct: 60 YQPLLWLLAGLFSVTLVTNAL------ETVLRVL-------NAHTEQRLEYHLEH----M 102 Query: 105 VNKKLSVISMEQLENPAVYDLLDRVQSN-VNKGILSYINNSLSVVLPVXXXXXXXXXXXX 163 V KK + + + ++P Y+ R+++ + + L + L + + Sbjct: 103 VAKKAASVPLSYFDDPEFYNHHHRIRAEAMGERFLDPLERVLDLGKNLITLISFAVFLFS 162 Query: 164 XXXXXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMF 223 ++LA +P L++ G Y+ A Q+ +R Y+ +L R +KE+R+F Sbjct: 163 IHWGLAVMSLLAVIPLLIVQIKFGMSEYYLARLQTPEIREAAYISSILNERDSSKEVRLF 222 Query: 224 GLIDYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMS 283 L D+ R + KE +LL IF + CL + + + + Sbjct: 223 QLKDFLLGRWSRLFLKNMKEVLRLLHRREKALILSGIFTSSMYIGCLGLVVSLLRASALM 282 Query: 284 IGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLE-----LPEDTGSGHKKI 338 +GD + + ++Q LS M ++ + N LYI D+ +FLE +P+ TG Sbjct: 283 VGDFVAIAQAVQNTQQLLSQMTFNLAHIVNRCLYIADLFDFLEYEDKTIPQLTGKAEFPT 342 Query: 339 VESPTIQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPT 398 I F V F YP + + + + TI +GE+IA+VG+NG GKTT + L+GLY Sbjct: 343 KLQSGITFDRVSFCYPKMERNVIDEVSFTIKRGERIAIVGDNGSGKTTLVKCLMGLYPIH 402 Query: 399 SGEVLVGGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHKQSLQIFSM 457 SG +L + ++ I D + +FQ+FI+Y + +NI G ++ + ++ ++ Sbjct: 403 SGNILFDNQSIRELDISDLQRNITVIFQDFIRYNFTAGENITIGRVTQEVNPAQMRQVAV 462 Query: 458 ----DSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASL 513 D+FI LP G DT LG+ + +LSGGQWQ++A+ARAL R G EI I+DEPTA+L Sbjct: 463 ETGADTFIRRLPSGYDTILGRTLQDGEDLSGGQWQKVALARALYRRG-EIFILDEPTAAL 521 Query: 514 DPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGK 573 DP+ E +++++F AL +NKTAI ISHR+ R+ D I+V QG++ E G H ELM+ G+ Sbjct: 522 DPQAELEVFQQFDALTQNKTAIFISHRMAAARMADRIFVMKQGQLIEVGHHDELMDANGE 581 Query: 574 YYQMYSAQSYLYA 586 Y +MY Q+ +A Sbjct: 582 YSRMYRMQAKWFA 594 >A5UR27.1 A5UR27_ROSS1 ABC transporter related Length = 625 Score = 261 bits (667), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 167/515 (32%), Positives = 260/515 (50%), Gaps = 23/515 (4%) Query: 83 EHITAHINNRLKYNINREFIRQVNKKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYIN 142 EH+ +N RL + IN + IR K LS + + E+ + YD L + + L IN Sbjct: 121 EHV---LNARLGHAINVDIIR---KALS-LDLSYFEDASFYDKLQNARREADFRALGIIN 173 Query: 143 NSLSVVLPVXXXXXXXXXXXXXXXXXXXXAVLATVPYLLLMNAQGKKS--YFQAVEQSKP 200 S SVV + AT+P + AQG+ S YF+ + P Sbjct: 174 GSYSVVQNLLTLLSFAVLLLAFNPWITLVLFGATIPAFV---AQGRYSHLYFRLLTWHAP 230 Query: 201 -LRRLNYMYDVLTSRRYAKEIRMFGLIDYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFID 259 RR+ Y+ VLT KE+++FGL + R + + + E L +L Sbjct: 231 EFRRMQYLEHVLTVDSSVKEVKLFGLGEPLLKRYSDTFQVFFCEDVDLARRRSLISVGWG 290 Query: 260 IFRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIE 319 + N++ + +++G + L +T + + + ++ + L+++ Sbjct: 291 VIANLSYYGAYAWIVFVTIAGTITLGSMTLYLTLFRQSQSAFQSLFSNVTRLYENGLFMD 350 Query: 320 DMMNFLELPEDTGSGHKKIVESPTIQ----FKHVDFKYPNSKTHTLKNIDITINKGEKIA 375 ++ +FL L + + P +Q F++V F+YP L+NI++TI GEK+A Sbjct: 351 NLFSFLSLQPHMSRATQPVAVPPRLQRGLEFRNVSFRYPGRDDWALRNINLTIAPGEKLA 410 Query: 376 LVGENGCGKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTVCVFQNFIKYQLSV 434 LVG NG GKTT I +L LY PT GE+L+ G L D ID+ R +FQ+F+KYQLS Sbjct: 411 LVGPNGAGKTTLIKLLTRLYDPTEGEILLDGVDLRDYDIDEVRRRIGVIFQDFVKYQLSA 470 Query: 435 ADNIKAGNFG----EDFHKQSLQIFSMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRL 490 +NI G E +++ Q D I LP G++T LG+ N ELSGGQWQ++ Sbjct: 471 RENIGFGQIDRLDDESRVREAAQRGGADDIIAELPAGMETMLGRWFENGFELSGGQWQKI 530 Query: 491 AIARALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTI 550 A++RA R G E+L++DEPTA+LD + E +I++ F L K A++ISHR R+ D I Sbjct: 531 ALSRAFMREG-EVLVLDEPTAALDAEREYEIFQRFRELTAGKIAVLISHRFSTVRMADRI 589 Query: 551 YVFDQGEICEFGSHAELMNRRGKYYQMYSAQSYLY 585 V + G I E GSHAEL+ + G Y +++ Q+ Y Sbjct: 590 AVIENGCITEIGSHAELLRQGGTYARLFEMQAEGY 624 >Q93GH7.1 Q93GH7_BACSU Putative SpaT Length = 614 Score = 254 bits (648), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 153/528 (28%), Positives = 274/528 (51%), Gaps = 10/528 (1%) Query: 65 LVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQVNKKLSVISMEQLENPAVYD 124 ++ I Y + E I+ + N + + NI + +V KK S ++++ ENP +YD Sbjct: 72 VISIFLTYLGVSFFSELISQISEYYNGKFQLNIGYKLNYKVMKKSSNLALKDFENPEIYD 131 Query: 125 LLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXXXXXXXAVLATVPYLLLMN 184 L+RV ++ I +++ ++ V L Sbjct: 132 KLERVTKEISYKPYQIIQAIITMTTSCVTLLSSIAFLMSWNPKVSLLLLVIPVISLFYFL 191 Query: 185 AQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLIDYFNDRTEEIRKDVWKEK 244 G++ +F +++ R+ Y+ +LT KE++++ L DY ++ +I+K + Sbjct: 192 KIGQQEFFIHWKRAGQERKSWYISYILTHDFSFKELKLYNLKDYLLNKYWDIKKTFIGQD 251 Query: 245 FKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGM 304 K+L T +I + + + + + ++ +G+VM I S+ + + + Sbjct: 252 TKILRKKTYLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNVMSYIRSVSLVQNHSQSI 311 Query: 305 VNKISSMNNYTLYIEDMMNFLELPEDTGSGHKKIVESP--TIQFKHVDFKYPNSKTHTLK 362 + I S+ N LY+ + FLEL E+ GHKK + P ++ F++V F YPN TLK Sbjct: 312 MTSIYSIYNSNLYMNQLYEFLELKEEKNEGHKKQIVEPIDSVVFQNVSFIYPNQGEQTLK 371 Query: 363 NIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTV 421 +I+++++KGE++A+VG NG GK+TFI +L GLY+ G++L+ G + ++ +D + Sbjct: 372 HINVSLHKGERVAIVGPNGSGKSTFIKLLTGLYEVQQGDILINGINIKELDMDSYMNQIA 431 Query: 422 CVFQNFIKYQLSVADNIKAGNFGEDFHK-----QSLQIFSMDSFIDNLPKGLDTQLGQLE 476 +FQ+F+KY++++ +NI G + H+ + L I +D + DTQLG Sbjct: 432 ALFQDFMKYEMTLKENIGFGQI-DKLHQTNKMHEVLDIVKVDFLKSHSSYQFDTQLGLWF 490 Query: 477 NNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIM 536 + +LSGGQWQ++A+ARA R + + I+DEP+A+LDP E +I++ F +L ++K I Sbjct: 491 DEGRQLSGGQWQKIALARAYFREAS-MYILDEPSAALDPIAEKEIFDTFFSLSKDKIGIF 549 Query: 537 ISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYSAQSYL 584 ISHRL +L D I V D GEI G H EL+ Y +M +++Y+ Sbjct: 550 ISHRLVAAKLADRIIVMDNGEIVGIGKHEELLKTCPLYKKMDESENYM 597 >P33116.1 SPAT_BACSU Subtilin transport ATP-binding protein spaT Length = 614 Score = 253 bits (647), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 155/545 (28%), Positives = 281/545 (51%), Gaps = 13/545 (2%) Query: 48 LIDDLAFVHSASAVTQKLVVIIAGYTVLKVLLEWSEHITAHINNRLKYNINREFIRQVNK 107 LI+ L + ++ ++ I Y + E I+ N + + NI + +V K Sbjct: 58 LINSLVTIRKEVSI---VITIFLTYLGVSFFSELISQISEFYNGKFQLNIGYKLNYKVMK 114 Query: 108 KLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXXX 167 K S ++++ ENP +YD L+RV ++ I +++ Sbjct: 115 KSSNLALKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSFVTLLSSIAFLMSWNPK 174 Query: 168 XXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLID 227 ++ V L G++ +F +++ R+ Y+ +LT KE++++ L D Sbjct: 175 VSLLLLVIPVISLFYFLKIGQEEFFIHWKRAGKERKSWYISYILTHDFSFKELKLYNLKD 234 Query: 228 YFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDV 287 Y ++ +I+K ++ K+L TL +I + + + + + ++ +G+V Sbjct: 235 YLLNKYWDIKKSFIEQDTKILRKKTLLNLIYEIAVQLVGAVIIFIAIMSAFAGKIMVGNV 294 Query: 288 MLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLELPEDTGSGHKKIVESP--TIQ 345 M I S+ + + ++ I S+ N LY+ + FLEL E+ GHKK + P ++ Sbjct: 295 MSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQGHKKPIVEPIHSVV 354 Query: 346 FKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVG 405 F++V F YPN TLK+I+++++KGE++A+VG NG GK TFI +L GLY+ G++L+ Sbjct: 355 FQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKKTFIKLLTGLYEVHEGDILIN 414 Query: 406 GDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHK-----QSLQIFSMDS 459 G + ++ +D + +FQ+F+KY++++ +NI G + H+ + L I D Sbjct: 415 GINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQI-DKLHQTNKMHEVLDIVRADF 473 Query: 460 FIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDPKVET 519 + DTQLG + +LSGGQWQ++A+ARA R + + I+DEP+++LDP E Sbjct: 474 LKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREAS-LYILDEPSSALDPIAEK 532 Query: 520 QIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYS 579 + ++ F +L ++K I ISHRL +L D I V D+GEI G+H EL+ Y +M Sbjct: 533 ETFDTFFSLSKDKIGIFISHRLVAAKLADRIIVMDKGEIVGIGTHEELLKTCPLYKKMDE 592 Query: 580 AQSYL 584 +++Y+ Sbjct: 593 SENYM 597 >B8HPX2.1 B8HPX2_CYAP4 ABC transporter related Length = 592 Score = 253 bits (645), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 159/493 (32%), Positives = 257/493 (52%), Gaps = 20/493 (4%) Query: 107 KKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXX 166 KK I +E ENP ++L+R + +N+ S + Sbjct: 105 KKAIEIDLELYENPQYQNILERAKWEAPHRPTQILNSLTSAGRSIISLIAVAGLLISLHW 164 Query: 167 XXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLI 226 +LA++P ++ Q K Y Q++ RR NY+ +L S R AKEIR+F L Sbjct: 165 GLLCILLLASIPAAMVRFRQSKVLYKWRRHQTEIERRANYIGHLLLSDRPAKEIRLFNLG 224 Query: 227 DYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMT----CIGVLENRM 282 +F R +I++ +++EK + + I + G+ +L T + ++ + Sbjct: 225 YFFIQRYHDIKQKLFEEKLAINTRQAIASLII----QASTGLVVLGTYAYIILQTIDGKF 280 Query: 283 SIGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLELPEDTGS-GHKKIVES 341 +GD++L + Q + ++ +S++ L++ D+ FL L + S H K Sbjct: 281 LLGDLVLYTQAFQRGQGAIRELIAGSASLHENNLFLADLFEFLGLQSNMPSPAHPKPFPR 340 Query: 342 PT---IQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPT 398 P I FK+V F+Y NS +K I++ I GE +ALVGENG GKTT + +L LY T Sbjct: 341 PIHEGIVFKNVSFQYLNSSRQAIKKINLCIAPGEIVALVGENGSGKTTLVKLLCRLYDVT 400 Query: 399 SGEVLVGGDPLA--DVIDDFRTMTVCVFQNFIKYQLSVADNIKAGNF----GEDFHKQSL 452 G + + G + V D R ++V +FQ+F +YQL+V DNI GN + ++ Sbjct: 401 DGSITIDGINIKHFSVTDLHRQISV-IFQDFTQYQLTVEDNIWLGNIHLPSSTELITRAA 459 Query: 453 QIFSMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTAS 512 Q D+ I +LP+G T LG+ ELSGGQWQ++A+ARA + +I+++DEPT++ Sbjct: 460 QKAGADTTIQSLPQGYKTLLGKWFQGGEELSGGQWQKIALARAFIK-DAQIIVLDEPTSA 518 Query: 513 LDPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRG 572 LD E +I+++F L +++T ++I+HRL ++ D IYV QGEI E GSHA+L+ RG Sbjct: 519 LDAYAEAEIFQKFRELMQDRTTLLITHRLSTVKMADRIYVMHQGEIVESGSHAQLIESRG 578 Query: 573 KYYQMYSAQSYLY 585 KY M+ Q+ Y Sbjct: 579 KYAYMFETQAKNY 591 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,117,438,305 Number of extensions: 124218153 Number of successful extensions: 679952 Number of sequences better than 1.0e-05: 73086 Number of HSP's gapped: 571652 Number of HSP's successfully gapped: 77356 Length of query: 586 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 445 Effective length of database: 1,732,333,088 Effective search space: 770888224160 Effective search space used: 770888224160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)