BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0015.p (384 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DFZ5.1 B1DFZ5_9BACL Glycosyl hydrolase family 88 501 e-140 Q9RC92.1 UGL_BACGL Unsaturated glucuronyl hydrolase EC=3.2.1.- 449 e-124 Q9KB74.1 Q9KB74_BACHD Unsaturated glucuronyl hydrolase 446 e-123 B3K5I5.1 B3K5I5_9BACI Glycosyl hydrolase family 88 446 e-123 B3K5Z2.1 B3K5Z2_9BACI Glycosyl hydrolase family 88 434 e-120 >B1DFZ5.1 B1DFZ5_9BACL Glycosyl hydrolase family 88 Length = 376 Score = 501 bits (1289), Expect = e-140, Method: Compositional matrix adjust. Identities = 244/368 (66%), Positives = 292/368 (79%) Query: 6 MWAQAIEDALRVVSHNIERFGDKFPVTSTQGDRIYRLTPGNDWTEGFWTGMLWLSYEYSR 65 MW +AIED L+V NIERFG KFP S Y L WTEGFW G+LWLSYEYS Sbjct: 1 MWKKAIEDTLQVTRANIERFGSKFPHVSENSSGQYVLIENEGWTEGFWPGILWLSYEYSG 60 Query: 66 DPRFRTAAMTTLDNFRERLEHRVGLEHHDIGFLYSPSIKAHWILERDESSRSAALQAADV 125 D +R AA+ T+++ +RLE+ + L+HHDIGFLYS S KA WILE+DE+++ + AA V Sbjct: 61 DRAYREAAVQTVNSLGKRLENNLSLDHHDIGFLYSLSSKAQWILEKDEAAKQITIDAAKV 120 Query: 126 LLERWREIPGILQAWGPKNDPENGGRIIIDCLMNLPLLHWAYQQTGREAYRQAAEQQTAM 185 L++RWRE ILQAWGPK+DP NGGRIIIDC+MNLPLLHWAY QTG +++R+AAEQ T M Sbjct: 121 LMKRWRENMNILQAWGPKDDPINGGRIIIDCMMNLPLLHWAYLQTGDKSFREAAEQHTEM 180 Query: 186 SRRFLVRGDDSTYHTFYFDPVTGDSIRGGTHQGSRDGSTWTRGQAWGIYGFALAYRYMRQ 245 SRRFLVRGDDS+YHTF+F+P TGDS RGGTHQG +DGSTWTRGQAWGIYGFALAYRY+R+ Sbjct: 181 SRRFLVRGDDSSYHTFWFNPETGDSNRGGTHQGFKDGSTWTRGQAWGIYGFALAYRYLRK 240 Query: 246 PGLLETSKRLARYFVDRLPEDSVVFWDFDVPQIPETKRDSSAAAIAASGMLELVELLPEH 305 P LET+KRLA YF DR+PED VV+WDFD PQ+PETKRDSSA+AIAASGMLE+ LP++ Sbjct: 241 PEYLETAKRLAIYFADRIPEDGVVYWDFDAPQVPETKRDSSASAIAASGMLEICGFLPDN 300 Query: 306 DADREWLAQAARRIVASLAANYSTKGEPDAQGLLNRGSYAVRLDISPDDYVIWGDYFYLE 365 D + L QA R + L ++YST GE +A+G LNRGSY+VR + SPDDY IWGDYFYLE Sbjct: 301 DPVYKKLMQAVNRSMEGLVSHYSTNGEKEAEGFLNRGSYSVRTNTSPDDYTIWGDYFYLE 360 Query: 366 ALMRLGRG 373 ALMRL +G Sbjct: 361 ALMRLDKG 368 >Q9RC92.1 UGL_BACGL Unsaturated glucuronyl hydrolase EC=3.2.1.- Length = 377 Score = 449 bits (1155), Expect = e-124, Method: Compositional matrix adjust. Identities = 223/368 (60%), Positives = 276/368 (75%), Gaps = 1/368 (0%) Query: 6 MWAQAIEDALRVVSHNIERFGDKFPVTSTQGDRIYRLTPGNDWTEGFWTGMLWLSYEYSR 65 MW QAI DAL + + N+++FGD+FP S G Y L DWT+GFW+G+LWL YEY+ Sbjct: 1 MWQQAIGDALGITARNLKKFGDRFPHVS-DGSNKYVLNDNTDWTDGFWSGILWLCYEYTG 59 Query: 66 DPRFRTAAMTTLDNFRERLEHRVGLEHHDIGFLYSPSIKAHWILERDESSRSAALQAADV 125 D ++R A+ T+ +FRERL+ L+HHDIGFLYS S KA WI+E+DES+R AL AADV Sbjct: 60 DEQYREGAVRTVASFRERLDRFENLDHHDIGFLYSLSAKAQWIVEKDESARKLALDAADV 119 Query: 126 LLERWREIPGILQAWGPKNDPENGGRIIIDCLMNLPLLHWAYQQTGREAYRQAAEQQTAM 185 L+ RWR GI+QAWGPK DPENGGRIIIDCL+NLPLL WA +QTG YR+ AE Sbjct: 120 LMRRWRADAGIIQAWGPKGDPENGGRIIIDCLLNLPLLLWAGEQTGDPEYRRVAEAHALK 179 Query: 186 SRRFLVRGDDSTYHTFYFDPVTGDSIRGGTHQGSRDGSTWTRGQAWGIYGFALAYRYMRQ 245 SRRFLVRGDDS+YHTFYFDP G++IRGGTHQG+ DGSTWTRGQAWGIYGFAL RY+ Sbjct: 180 SRRFLVRGDDSSYHTFYFDPENGNAIRGGTHQGNTDGSTWTRGQAWGIYGFALNSRYLGN 239 Query: 246 PGLLETSKRLARYFVDRLPEDSVVFWDFDVPQIPETKRDSSAAAIAASGMLELVELLPEH 305 LLET+KR+AR+F+ R+PED VV+WDF+VPQ P + RDSSA+AI A G+LE+ L E Sbjct: 240 ADLLETAKRMARHFLARVPEDGVVYWDFEVPQEPSSYRDSSASAITACGLLEIASQLDES 299 Query: 306 DADREWLAQAARRIVASLAANYSTKGEPDAQGLLNRGSYAVRLDISPDDYVIWGDYFYLE 365 D +R+ AA+ V +L Y+ + + +A+G + RGSY VR ISPDDY IWGDY+YLE Sbjct: 300 DPERQRFIDAAKTTVTALRDGYAERDDGEAEGFIRRGSYHVRGGISPDDYTIWGDYYYLE 359 Query: 366 ALMRLGRG 373 AL+RL RG Sbjct: 360 ALLRLERG 367 >Q9KB74.1 Q9KB74_BACHD Unsaturated glucuronyl hydrolase Length = 375 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 209/367 (56%), Positives = 269/367 (73%), Gaps = 3/367 (0%) Query: 6 MWAQAIEDALRVVSHNIERFGDKFPVTSTQGDRIYRLTPGNDWTEGFWTGMLWLSYEYSR 65 MW QA+ D NI+RF +FP S G+ Y L N+WT GFW+G+LWL YEY+ Sbjct: 2 MWKQAMTDVAEKTLTNIKRFNGRFPHVSEDGEH-YELNNNNEWTNGFWSGILWLCYEYTN 60 Query: 66 DPRFRTAAMTTLDNFRERLEHRVGLEHHDIGFLYSPSIKAHWILERDESSRSAALQAADV 125 DP FR AA +T+ +F++R+E + L+HHDIGFLYS S KA WI+ERDE ++ ++AADV Sbjct: 61 DPAFRQAAASTVRSFQQRMEQNLELDHHDIGFLYSLSSKAQWIIERDERAKQLTIEAADV 120 Query: 126 LLERWREIPGILQAWGPKNDPENGGRIIIDCLMNLPLLHWAYQQTGREAYRQAAEQQTAM 185 L++RWRE + QAWGP+ D NGGRII+DCLMNLPLL WA + TG YR+AA Sbjct: 121 LMKRWREKIELFQAWGPEGDLSNGGRIIVDCLMNLPLLFWASEVTGNPDYREAAIIHADK 180 Query: 186 SRRFLVRGDDSTYHTFYFDPVTGDSIRGGTHQGSRDGSTWTRGQAWGIYGFALAYRYMRQ 245 +RRF+VRGDDSTYHTFYF+ TG+++RGGTHQG DGSTW+RGQAW IYGFA+AYRY Sbjct: 181 TRRFIVRGDDSTYHTFYFNQETGEALRGGTHQGYEDGSTWSRGQAWAIYGFAIAYRYTGN 240 Query: 246 PGLLETSKRLARYFVDRLPEDSVVFWDFDVPQIPETKRDSSAAAIAASGMLELVELLPEH 305 LET+KR A+YF++ LP D V +WDF+ P P+TKRDSSA+AIA+ G+LEL+ L E Sbjct: 241 ERYLETAKRTAKYFIENLPADYVAYWDFNAPITPDTKRDSSASAIASCGILELLSHLQET 300 Query: 306 DADREWLAQAARRIVASLAANYSTKGEPDAQGLLNRGSYAVRLDISPDDYVIWGDYFYLE 365 D D+ + Q+ ++ + SL NY++ E DAQGL+ RGSY+VR+ +PDDYVIWGDYFY E Sbjct: 301 DPDKAFFQQSVQKQMTSLVENYAS--EKDAQGLIKRGSYSVRIGHAPDDYVIWGDYFYTE 358 Query: 366 ALMRLGR 372 ALMRL + Sbjct: 359 ALMRLEK 365 >B3K5I5.1 B3K5I5_9BACI Glycosyl hydrolase family 88 Length = 386 Score = 446 bits (1146), Expect = e-123, Method: Compositional matrix adjust. Identities = 216/367 (58%), Positives = 262/367 (71%), Gaps = 1/367 (0%) Query: 7 WAQAIEDALRVVSHNIERFGDKFPVTSTQGDRIYRLTPGNDWTEGFWTGMLWLSYEYSRD 66 W IE L NI RF D+FP G+ YRL DWTEGFW G+LWLS EY +D Sbjct: 11 WNAVIEQTLHTTKANIARFEDRFPHVGN-GNGKYRLVDHTDWTEGFWPGILWLSCEYGQD 69 Query: 67 PRFRTAAMTTLDNFRERLEHRVGLEHHDIGFLYSPSIKAHWILERDESSRSAALQAADVL 126 R AA+ +F+ RL L+HHDIGFLYS S A W++ERDE+++ ALQAAD L Sbjct: 70 SYIREAAVRCTHSFQARLAEHKNLDHHDIGFLYSLSALAQWMVERDEAAKITALQAADAL 129 Query: 127 LERWREIPGILQAWGPKNDPENGGRIIIDCLMNLPLLHWAYQQTGREAYRQAAEQQTAMS 186 + RWR ++QAWGP++DP+NGGRIIIDCLMNLPLL WA +QTGR AY +AA S Sbjct: 130 MRRWRPTLQLIQAWGPEHDPQNGGRIIIDCLMNLPLLCWATEQTGRSAYAEAARIHADKS 189 Query: 187 RRFLVRGDDSTYHTFYFDPVTGDSIRGGTHQGSRDGSTWTRGQAWGIYGFALAYRYMRQP 246 +FLVRGD S+YHTFYFDP +GD++RGGTHQG +DGSTW+RGQAWGIYGFALAYRY +P Sbjct: 190 LKFLVRGDASSYHTFYFDPESGDAVRGGTHQGFQDGSTWSRGQAWGIYGFALAYRYFEEP 249 Query: 247 GLLETSKRLARYFVDRLPEDSVVFWDFDVPQIPETKRDSSAAAIAASGMLELVELLPEHD 306 L+T+ R+ARYF+DRLP+D V +WDFDVP T +DSSA+AIAA GMLE+ + L D Sbjct: 250 QYLDTAIRMARYFIDRLPDDHVAYWDFDVPPTALTPKDSSASAIAACGMLEIADQLSSDD 309 Query: 307 ADREWLAQAARRIVASLAANYSTKGEPDAQGLLNRGSYAVRLDISPDDYVIWGDYFYLEA 366 RE+L A RR + S+ Y TKG PD +G L GSY VR I+PDD+VIWGDYF+LEA Sbjct: 310 PRREFLLDAVRRSMDSMVQGYFTKGNPDEEGFLRHGSYFVRGGIAPDDFVIWGDYFFLEA 369 Query: 367 LMRLGRG 373 LMRL G Sbjct: 370 LMRLNNG 376 >B3K5Z2.1 B3K5Z2_9BACI Glycosyl hydrolase family 88 Length = 373 Score = 434 bits (1116), Expect = e-120, Method: Compositional matrix adjust. Identities = 213/368 (57%), Positives = 264/368 (71%), Gaps = 3/368 (0%) Query: 6 MWAQAIEDALRVVSHNIERFGDKFPVTSTQGDRIYRLTPGNDWTEGFWTGMLWLSYEYSR 65 MW+ AI+DA+ + N++ FG +FP S G Y L +DWT GFW+G+LWL YEY+ Sbjct: 1 MWSNAIQDAVEKIRKNMDAFGARFPHVSRDG--AYLLNDNDDWTNGFWSGLLWLCYEYTG 58 Query: 66 DPRFRTAAMTTLDNFRERLEHRVGLEHHDIGFLYSPSIKAHWILERDESSRSAALQAADV 125 D FR A T+++FR R + L+HHDIGFLYS S KA WI+E+DES+R LQAAD+ Sbjct: 59 DEAFRLGAKRTVEDFRRRFAEKKVLDHHDIGFLYSLSSKAEWIVEKDESARQLTLQAADL 118 Query: 126 LLERWREIPGILQAWGPKNDPENGGRIIIDCLMNLPLLHWAYQQTGREAYRQAAEQQTAM 185 LL+RWR G +QAWGP+ D + GGRIIIDCLMNLPLL WA QQTG+ YR+ A Q Sbjct: 119 LLKRWRPSGGYIQAWGPEGDEQEGGRIIIDCLMNLPLLFWAGQQTGKSQYREIAIMQAEK 178 Query: 186 SRRFLVRGDDSTYHTFYFDPVTGDSIRGGTHQGSRDGSTWTRGQAWGIYGFALAYRYMRQ 245 +RR+LVRGDDS+YHTF+FDP TG+ I G THQG +GSTWTRGQAWG+YGFALAYRY Sbjct: 179 TRRYLVRGDDSSYHTFFFDPHTGEPIGGATHQGYTNGSTWTRGQAWGVYGFALAYRYTGD 238 Query: 246 PGLLETSKRLARYFVDRLPEDSVVFWDFDVPQIPETKRDSSAAAIAASGMLELVELLPEH 305 L+TSKR+A+YF+ LPED V WDF+ P ET RDSSA+AI A+G+LELV L E Sbjct: 239 HAYLDTSKRMAKYFLKHLPEDHVALWDFNAPVTAETYRDSSASAIVAAGLLELVGHLEEG 298 Query: 306 DADREWLAQAARRIVASLAANYSTKGEPDAQGLLNRGSYAVRLDISPDDYVIWGDYFYLE 365 D DR Q + + SL +YST G+ +A+GLL GSY VR +SPDD++IWGDYFYLE Sbjct: 299 DPDRAEFEQGLKDSMTSLVRHYSTIGQ-EAEGLLQHGSYHVRGGLSPDDFMIWGDYFYLE 357 Query: 366 ALMRLGRG 373 AL+RL G Sbjct: 358 ALIRLETG 365 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.136 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,468,654,703 Number of extensions: 108239255 Number of successful extensions: 224578 Number of sequences better than 1.0e-05: 304 Number of HSP's gapped: 223665 Number of HSP's successfully gapped: 304 Length of query: 384 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 247 Effective length of database: 1,770,017,148 Effective search space: 437194235556 Effective search space used: 437194235556 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)