BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0011.p (378 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DB44.1 B1DB44_9BACL Beta-lactamase 598 e-169 B1DE16.1 B1DE16_9BACL Beta-lactamase 548 e-154 B8I688.1 B8I688_CLOCE Beta-lactamase 312 3e-83 A9KLC3.1 A9KLC3_CLOPH Beta-lactamase 196 2e-48 Q15PT0.1 Q15PT0_PSEA6 Beta-lactamase 196 2e-48 >B1DB44.1 B1DB44_9BACL Beta-lactamase Length = 375 Score = 598 bits (1541), Expect = e-169, Method: Compositional matrix adjust. Identities = 277/375 (73%), Positives = 329/375 (87%) Query: 4 FEPLSSFIDRITSWRIPWAEVLVMHRNEPVYRYRNGYASLEEKTPIDDGRIINLYSLTKI 63 + L+ FIDR+TSWRIPWAEV+V+HRNE ++RYR+G+++LE++TPI++GRIINLYS+TKI Sbjct: 1 MKALTEFIDRLTSWRIPWAEVMVIHRNETLFRYRSGFSNLEDETPIEEGRIINLYSMTKI 60 Query: 64 MTCTAALQLLEKGAFLLNDRLSDYLPEYTEMNVKKRLPGGELAFERAVNPITVRDLFTMS 123 MTC AAL+L+E+G LLND LS YLPEY +MN++KRLP G+L ERA I VRDLFTM+ Sbjct: 61 MTCVAALKLVEQGKMLLNDPLSAYLPEYADMNLRKRLPDGKLEIERAKKTILVRDLFTMT 120 Query: 124 AGFSYDLELPSIKEAVSRTGGKMPTRAIAEALAKEPLQFEPGTRWNYSLCHDVLAALVEA 183 AGFSYD+ PS+KE +T G++PTRA AEALAKEPL FEPGTRWNYSLCHDVLAALVE Sbjct: 121 AGFSYDIGSPSLKEIAEQTQGRVPTRAFAEALAKEPLLFEPGTRWNYSLCHDVLAALVEV 180 Query: 184 VDGRRFGTYIREEITGPLGMTDTGFDLPDGKRSRLAPQYEYSDELGKPVRKDGNGFRLGT 243 VDGRRFG IREEITGPLGM DT FDL + +RSR+ PQ+E+ DELG+ VRKDGNG+RLGT Sbjct: 181 VDGRRFGACIREEITGPLGMKDTAFDLSEEQRSRVLPQFEFKDELGRAVRKDGNGYRLGT 240 Query: 244 EYESGGAGLLSTVTDYAQFLNTLTNLGTSPDGVRILSPASVELMRTEQLTESMRGDFSWE 303 E+ESGGAGLLSTV+DYA FLN LTN GTSPDGVRILSPA ++LMRT+ LT+ MRGD++WE Sbjct: 241 EHESGGAGLLSTVSDYALFLNALTNKGTSPDGVRILSPAMIDLMRTDHLTDRMRGDYNWE 300 Query: 304 QLKGYSYGLGVRTHISQARSGSLSPLGEFGWSGAAGCLAVIDPASQLTVMYAQHMLNNQE 363 QLKGY YGLGVRTH S+A+SGSLSPLGEFGWSGAAGC+A+IDP S LTVMYAQH+LNNQE Sbjct: 301 QLKGYGYGLGVRTHASKAQSGSLSPLGEFGWSGAAGCMAIIDPDSGLTVMYAQHLLNNQE 360 Query: 364 PFVHPRLRNIVYACL 378 P++HPRLRNIVY+ L Sbjct: 361 PYIHPRLRNIVYSGL 375 >B1DE16.1 B1DE16_9BACL Beta-lactamase Length = 378 Score = 548 bits (1411), Expect = e-154, Method: Compositional matrix adjust. Identities = 270/377 (71%), Positives = 309/377 (81%) Query: 2 MNFEPLSSFIDRITSWRIPWAEVLVMHRNEPVYRYRNGYASLEEKTPIDDGRIINLYSLT 61 M+F PLS FIDRIT+WRIPWAEVLVMHRNE V+RYR GY LE KTP+D+ II LYSLT Sbjct: 1 MDFTPLSRFIDRITAWRIPWAEVLVMHRNETVFRYRTGYTDLEAKTPVDENGIIYLYSLT 60 Query: 62 KIMTCTAALQLLEKGAFLLNDRLSDYLPEYTEMNVKKRLPGGELAFERAVNPITVRDLFT 121 KI+TC AALQL+EKGA LL D +SDYLPE+ EM VKK+ E E + PITVRDLF Sbjct: 61 KILTCAAALQLVEKGAILLGDPISDYLPEFREMAVKKKQSDDESTTELSERPITVRDLFA 120 Query: 122 MSAGFSYDLELPSIKEAVSRTGGKMPTRAIAEALAKEPLQFEPGTRWNYSLCHDVLAALV 181 M+AGFSYDL PSIKEA RT G++PTR A+ALAKEPL FEPGTRWNYSLCHDVLAALV Sbjct: 121 MTAGFSYDLVSPSIKEAAERTNGRLPTRDFAKALAKEPLLFEPGTRWNYSLCHDVLAALV 180 Query: 182 EAVDGRRFGTYIREEITGPLGMTDTGFDLPDGKRSRLAPQYEYSDELGKPVRKDGNGFRL 241 E +DGRRFGTY+REEIT PLGM DTGF+L D +RSRLAPQY Y D GK V DG+ +R+ Sbjct: 181 EEIDGRRFGTYVREEITEPLGMLDTGFNLSDEQRSRLAPQYVYDDTQGKAVLMDGDWYRI 240 Query: 242 GTEYESGGAGLLSTVTDYAQFLNTLTNLGTSPDGVRILSPASVELMRTEQLTESMRGDFS 301 GTE+ESGGAGLLSTV+DY+ FLN LTN GTSP+GVRILS ASV+LMR + LT R D++ Sbjct: 241 GTEFESGGAGLLSTVSDYSLFLNMLTNRGTSPEGVRILSAASVDLMRADHLTGHTRSDYN 300 Query: 302 WEQLKGYSYGLGVRTHISQARSGSLSPLGEFGWSGAAGCLAVIDPASQLTVMYAQHMLNN 361 W GY YGLGVRTHIS++ SGSLS LGEFGWSGA GC A+IDP S+LTVMYAQH++NN Sbjct: 301 WAHHAGYGYGLGVRTHISKSGSGSLSSLGEFGWSGAGGCYAIIDPESRLTVMYAQHLVNN 360 Query: 362 QEPFVHPRLRNIVYACL 378 QEP+VHPRL+NIVY+CL Sbjct: 361 QEPYVHPRLKNIVYSCL 377 >B8I688.1 B8I688_CLOCE Beta-lactamase Length = 377 Score = 312 bits (800), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 158/381 (41%), Positives = 240/381 (62%), Gaps = 10/381 (2%) Query: 2 MNFEPLSSFIDRITSWRIPWAEVLVMHRNEPVYRYRNGYASLEEKTPIDDGRIINLYSLT 61 MN L ++D + S +P + ++ H+++PV+R+ G+A + + + L+S T Sbjct: 1 MNKAILKDYLDSLESKGVPGCDCVIFHKHKPVFRHTTGFADAGKTKHLTSENLFWLFSAT 60 Query: 62 KIMTCTAALQLLEKGAFLLNDRLSDYLPEYTEMNVKKRLPGGELAFERAVNPITVRDLFT 121 K++TCTA +QL+EKG L+ ++DYLPEY +NVK G E+ A N +T+R L + Sbjct: 61 KLITCTAIMQLVEKGKVSLDAPVADYLPEYKHLNVK---CGSEVV--PAKNTLTIRHLLS 115 Query: 122 MSAGFSYDLELPSIKEAVSRTGGKMPTRAIAEALAKEPLQFEPGTRWNYSLCHDVLAALV 181 M +G +Y+L+ SI + + T + TR + ALA EPL+FEPGT + YSL HDVL A++ Sbjct: 116 MQSGLNYNLQASSILKVLEDTEYEATTRQVIRALANEPLEFEPGTHFLYSLSHDVLGAVI 175 Query: 182 EAVDGRRFGTYIREEITGPLGMTDTGFDLPDGKRSRLAPQYEYSDEL--GKPVRKDGNGF 239 E V G++FG Y+ E I PLGM +TGF+L +++ ++ Q+EY + KP+ N + Sbjct: 176 EEVSGQKFGEYLEEHILKPLGMKNTGFELTPDRKANMSEQFEYDMDTMTSKPISL-ANCY 234 Query: 240 RLGTEYESGGAGLLSTVTDYAQFLNTLTNLGTSPDGVRILSPASVELMRTEQLTESMRGD 299 +L ++YESGGAGLLST DY FL+ + N G S DG R+L+ S+++MR +Q+ + + D Sbjct: 235 KLSSKYESGGAGLLSTADDYILFLDAMCNDGVSADGYRVLTRESIDMMRKDQMNDISKKD 294 Query: 300 FSWEQLKGYSYGLGVRTHISQARSGSLSPLGEFGWSGAAGCLAVIDPASQLTVMYAQHML 359 F GYSYGLGVRT + + RSG SPLGEFGW GAAG A+ID ++L + Y QH+ Sbjct: 295 FDEFGRIGYSYGLGVRTLVEKERSGVKSPLGEFGWDGAAGAYALIDVKNRLAIFYVQHVR 354 Query: 360 NNQEPF--VHPRLRNIVYACL 378 + +HP++R++ Y L Sbjct: 355 GCGYAYSDIHPKIRDLSYEML 375 >A9KLC3.1 A9KLC3_CLOPH Beta-lactamase Length = 382 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 128/392 (32%), Positives = 199/392 (50%), Gaps = 33/392 (8%) Query: 4 FEPLSSFID-RITSWRIPWAEVLVMHRNEPVYRYRNGYASLEEKTPIDDGRIINLYSLTK 62 E L+ ++ ++S ++ +LV H+NE V+++ G + I ++S+TK Sbjct: 6 LEQLTEILESEVSSGKLKGCSLLVQHKNERVFQHSFGTDQCDS--------IYRIFSMTK 57 Query: 63 IMTCTAALQLLEKGAFLLNDRLSDYLPEYTEMNVKKRLPGGELAFERAVNP-ITVRDLFT 121 +T A + L E+G L D +S YL + V + ER V +T++ L Sbjct: 58 PVTAIATMILYERGLLDLFDPISKYLKGFCNQTVL------DTEGERPVKKEVTIQHLLN 111 Query: 122 MSAGFSYDLEL-------PSIKEAVSRTGGKMPTRAIAEALAKEPLQFEPGTRWNYSLCH 174 M++G Y +L + E + T I+ + + PL FEPG W Y Sbjct: 112 MTSGIVYPEKLYPAGAKFEELVEQFKKDNTHPTTIQISNLVGQAPLAFEPGQEWAYGASA 171 Query: 175 DVLAALVEAVDGRRFGTYIREEITGPLGMTDTGFDLPDGKRSRLAPQYEYSDELGKPVR- 233 DVL +VE + G R+G ++++EI PLGM DTGF L D K+ RL P Y+ D G V Sbjct: 172 DVLGGIVETISGMRYGDFLKKEIFEPLGMKDTGFYLEDDKKERLVPMYK-KDADGNLVEI 230 Query: 234 -KDG--NGFRLGTE----YESGGAGLLSTVTDYAQFLNTLTNLGTSPDGVRILSPASVEL 286 KD N F + + YE GGAGL STV DY++F L + GT GVRIL ++E Sbjct: 231 TKDDLTNHFVVNPDSKPSYELGGAGLYSTVEDYSKFAGMLLSYGTYK-GVRILGRKTIEF 289 Query: 287 MRTEQLTESMRGDFSWEQLKGYSYGLGVRTHISQARSGSLSPLGEFGWSGAAGCLAVIDP 346 + QLT + + ++ + GY YG +R + Q+ +GS +GE+GW G G ++DP Sbjct: 290 ISQNQLTSNQQKSIWFDSMYGYGYGNLMRIMMDQSLAGSNGSIGEYGWDGLPGTYFMVDP 349 Query: 347 ASQLTVMYAQHMLNNQEPFVHPRLRNIVYACL 378 +LT++Y Q L+ + + ++R IVY L Sbjct: 350 TEELTLIYMQQNLHGADQSLRRKMRQIVYGSL 381 >Q15PT0.1 Q15PT0_PSEA6 Beta-lactamase Length = 419 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 126/383 (32%), Positives = 196/383 (51%), Gaps = 16/383 (4%) Query: 4 FEP-LSSFIDRITSWRIPWAEVLVMHRNEPVYRYRNGYASLEEKTPIDDGRIINLYSLTK 62 EP + +F+D ++ LV + + V+ G+ E KTP+ + I +YS+TK Sbjct: 43 IEPVMQAFVDE---QKLAGTLTLVARKGKVVHLEGVGFRDREAKTPMTEDTIFRIYSMTK 99 Query: 63 IMTCTAALQLLEKGAFLLNDRLSDYLPEYTEMNVKKRLPGGELAFERAVNPITVRDLFTM 122 +T AAL L E+G F +ND +S YLPE + V + G + E +T++ LFT Sbjct: 100 PITAVAALTLWEQGKFQMNDPISKYLPELANLKVFAGMDGENMILEDTNKVMTIKQLFTH 159 Query: 123 SAGFSYDLELPSIKEAVSRTG---GKMPTRAIAEALAKEPLQFEPGTRWNYSLCHDVLAA 179 SAGFSY + + ++ G+ + + +AK PL +PGT+WNYS+ D++ Sbjct: 160 SAGFSYGFSQSPVDKLYQQSALFSGETKREDLLKEVAKLPLNHQPGTKWNYSIGTDIIGV 219 Query: 180 LVEAVDGRRFGTYIREEITGPLGMTDTGFDLPDGKRSRLAPQYEYSDELGK--PVRKDGN 237 LVE + G+ G Y + I PL M+DTGF +P K+ RLA Y + + G+ P+ + Sbjct: 220 LVERLSGQTLGEYFEQHIFKPLKMSDTGFYVPKSKQDRLAQIYVINQQ-GQTVPMENEPL 278 Query: 238 GFRLGT-EYESGGAGLLSTVTDYAQFLNTLTNLGTSPDGVRILSPASVELMRTEQLTESM 296 G L E ESGG GL+ST+ DY F L N G + RIL +VE MRT L S+ Sbjct: 279 GDYLSAPEIESGGGGLVSTIADYYTFAQMLLNGGEYKEK-RILGRKTVEYMRTNHLPASL 337 Query: 297 RGDFSWE-QLKGYSYGLGVRTHISQARSGSLSPLGEFGWSGAAGCLAVIDPASQLTVMYA 355 +E G YGL + + + + ++ G++GW+GAA IDP Q+ V+ Sbjct: 338 ---IPFEPSSTGEGYGLAMSVTVDEQGANTMGSKGDYGWAGAASTYFRIDPKEQMIVIAM 394 Query: 356 QHMLNNQEPFVHPRLRNIVYACL 378 ++ + + +N+ Y L Sbjct: 395 TQLMPSSFFSYNKDFKNMAYQAL 417 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,373,203,872 Number of extensions: 103194782 Number of successful extensions: 244604 Number of sequences better than 1.0e-05: 1532 Number of HSP's gapped: 242955 Number of HSP's successfully gapped: 1542 Length of query: 378 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 241 Effective length of database: 1,770,017,148 Effective search space: 426574132668 Effective search space used: 426574132668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)