BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0008.p (372 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KI89.1 B3KI89_9BACI Regulatory protein LacI 474 e-132 A6M065.1 A6M065_CLOB8 Regulatory protein, LacI 347 8e-94 B7XZR2.1 B7XZR2_RUMHA Putative uncharacterized protein 320 1e-85 C0C788.1 C0C788_9FIRM Putative uncharacterized protein 286 3e-75 C5EMA5.1 C5EMA5_9FIRM Regulatory protein 200 1e-49 >B3KI89.1 B3KI89_9BACI Regulatory protein LacI Length = 346 Score = 474 bits (1220), Expect = e-132, Method: Compositional matrix adjust. Identities = 222/344 (64%), Positives = 273/344 (79%), Gaps = 2/344 (0%) Query: 25 MARDRVTIQDIADALGISRNTASKALNGVDSIPAETRDKVIKKAVELKYKQFSYMETTIG 84 M +++VTIQDIADALGISRNTASKALNG +SIP ETR KVI+KA+ELKYKQF++MET Sbjct: 1 MTKEKVTIQDIADALGISRNTASKALNGSESIPDETRSKVIRKAIELKYKQFAFMETDNL 60 Query: 85 TSEKQGNIALLTCNLPNGSHFGSLLISGLEKEISTKGYTLSIYFVRENDINGMTLPGNFE 144 ++ GNIALLT NLP+ SHFGS LISGLEK IS +GY LSI+ VR+ D + TLP NFE Sbjct: 61 LTKNPGNIALLTANLPSTSHFGSRLISGLEKRISAEGYNLSIHIVRDIDRSSRTLPNNFE 120 Query: 145 PSSVDGIICIEMFNKAYSKLITDLGIPTIFIDCAADMVYPELKADLVLMENEHSTYSVTR 204 S VDGIICIE+F+ YS+ +T LG+PT+FIDCAADM YP+L+ADL+LMENEHST +T+ Sbjct: 121 SSKVDGIICIELFDMEYSRELTSLGLPTVFIDCAADMFYPDLQADLLLMENEHSTCHMTK 180 Query: 205 KLIDHGYTSFGFVGDYNHCKSFNERWTGFNRALTECGITLDPAHNIVAPDRNFNINMEEN 264 +LI+ GY S GF+GD HCKSFNERW GFNRALTE GI LDP ++ PDR F E + Sbjct: 181 RLIEQGYASLGFIGDIQHCKSFNERWVGFNRALTEMGIRLDPEQCVLNPDREF--FSESD 238 Query: 265 WMGHQLDALEAWPSVFICANDFIAISVMKSLKNKGIKVPEEVAVCGFDDASESRVVEPHL 324 WM +L ++ PS F+CANDFIA++VMK+LKN I +P ++AVCGFD+A ESR+VEPHL Sbjct: 239 WMEKRLKQMKPLPSAFVCANDFIAVNVMKALKNSHIAIPGDIAVCGFDNAPESRIVEPHL 298 Query: 325 TTVHIYSSQMGIISAEILLSRIKDNTKPYQVTHVATDMIFRGST 368 +TVHIYS +MGI +A +LLSR+K+ PYQVTH+ T IFR ST Sbjct: 299 STVHIYSDEMGIAAANMLLSRVKEPALPYQVTHIQTKPIFRAST 342 >A6M065.1 A6M065_CLOB8 Regulatory protein, LacI Length = 354 Score = 347 bits (891), Expect = 8e-94, Method: Compositional matrix adjust. Identities = 170/350 (48%), Positives = 244/350 (69%), Gaps = 5/350 (1%) Query: 25 MARDRVTIQDIADALGISRNTASKALNGVDSIPAETRDKVIKKAVELKYKQFSYMETTIG 84 M ++T+QDIAD LGISRNT SKALN ++ T+ K+I+KA+E+ YKQF+Y+ Sbjct: 1 MLNKKITVQDIADELGISRNTVSKALNNTGTLAETTKSKIIQKAIEMGYKQFAYVNNVSS 60 Query: 85 TSEKQG---NIALLTCNLPNGSHFGSLLISGLEKEISTKGYTLSIYFVRENDINGMTLPG 141 S IALLT ++PN SHFGS L+SG E+++ST GY LS+Y VR+N++N +LP Sbjct: 61 ISPNSSLNKEIALLTSSMPNSSHFGSHLLSGFEEKMSTLGYRLSMYVVRDNELNSSSLPS 120 Query: 142 NFEPSSVDGIICIEMFNKAYSKLITDLGIPTIFIDCAADMVYPELKADLVLMENEHSTYS 201 NF+P VDGIICIEMF+K YS+LI L IPT+FID + + AD++LMEN HSTY+ Sbjct: 121 NFKPELVDGIICIEMFDKNYSELICGLDIPTLFIDSPSTHNSKPVNADILLMENFHSTYN 180 Query: 202 VTRKLIDHGYTSFGFVGDYNHCKSFNERWTGFNRALTECGITLDPAHNIVAPDRNFNINM 261 + + LI++ T+ GFVGD HC+SF ERW G+ A+ + ++ D ++I+ D+ + Sbjct: 181 IIKTLINNNKTNIGFVGDIYHCESFYERWKGYCSAILDSKLSFDINNSIIENDKEPYDDP 240 Query: 262 EENWMGHQLDALEAWPSVFICANDFIAISVMKSLKNKGIKVPEEVAVCGFDDASESRVVE 321 E W+G+++ L P V +CANDFIAI+V+++LK+K I VP+++ +CGFDD+ ES+++E Sbjct: 241 E--WLGNKIMDLSVLPQVLVCANDFIAINVIRALKHKSISVPDDILICGFDDSMESKIIE 298 Query: 322 PHLTTVHIYSSQMGIISAEILLSRIKDNTKPYQVTHVATDMIFRGSTPAL 371 PHLTTV I S +MG I+A++LLSRI + + P++ HV T FR ST L Sbjct: 299 PHLTTVSIPSYEMGDIAADLLLSRIDNPSIPFRTIHVRTSPKFRESTGNL 348 >B7XZR2.1 B7XZR2_RUMHA Putative uncharacterized protein Length = 341 Score = 320 bits (821), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 157/341 (46%), Positives = 232/341 (68%), Gaps = 6/341 (1%) Query: 29 RVTIQDIADALGISRNTASKALNGVDSIPAETRDKVIKKAVELKYKQFSYMETTIGTSEK 88 R+TIQDIAD+LG+SRNT SKA+N + TRDK+++KA EL YKQFSY+ + + Sbjct: 4 RITIQDIADSLGVSRNTVSKAINNTGVLADATRDKILQKAAELGYKQFSYLSPAVSVPKP 63 Query: 89 Q-GNIALLTCNLPNGSHFGSLLISGLEKEISTKGYTLSIYFVRENDINGMTLPGNFEPSS 147 + IAL T +PN SHFGS L++ +++IS+ GY LS Y +RE + + LP F+PS+ Sbjct: 64 EICEIALFTRAMPNSSHFGSKLLNTFQEKISSNGYRLSFYLIREQEFTDLLLPAGFDPSN 123 Query: 148 VDGIICIEMFNKAYSKLITDLGIPTIFIDCAADMVYPELKADLVLMENEHSTYSVTRKLI 207 GIIC+E+F+ AYS++++ L IP +F DCAA++ + + AD +LMEN +S S+ + LI Sbjct: 124 SAGIICLELFDCAYSQMLSKLDIPLLFTDCAANIDFTTIDADFLLMENRNSISSIVQSLI 183 Query: 208 DHGYTSFGFVGDYNHCKSFNERWTGFNRALTECGITLDPAHNIVAPDRNFNINMEENWMG 267 +G F G+ N+C+SF ER+ GF AL+ G L P +I+ D F+ +E+ + Sbjct: 184 KNGQHRLAFAGNINNCQSFFERYQGFCDALSLSG--LKPVTDILLADEPFH-TIEQ--LA 238 Query: 268 HQLDALEAWPSVFICANDFIAISVMKSLKNKGIKVPEEVAVCGFDDASESRVVEPHLTTV 327 + L P FICANDF+A+ ++K+LK G+ VPE+V + GFD+++ESR++EPHLTTV Sbjct: 239 SYIRQLPELPDAFICANDFVAMDLIKALKQCGLSVPEDVKITGFDNSAESRIIEPHLTTV 298 Query: 328 HIYSSQMGIISAEILLSRIKDNTKPYQVTHVATDMIFRGST 368 HI SS MG I+AE+LLSRI++ PY+ T+V T + +R S+ Sbjct: 299 HIPSSAMGYIAAELLLSRIEEPGIPYRTTYVRTVIKYRESS 339 >C0C788.1 C0C788_9FIRM Putative uncharacterized protein Length = 341 Score = 286 bits (731), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 145/344 (42%), Positives = 208/344 (60%), Gaps = 4/344 (1%) Query: 25 MARDRVTIQDIADALGISRNTASKALNGVDSIPAETRDKVIKKAVELKYKQFSYMETTIG 84 MA ++T+QDIADA G+SRNT SK NG S+P TR +V++KA + Y+ E Sbjct: 1 MAAKKITMQDIADACGLSRNTVSKVFNGRGSVPETTRQQVLEKARGMGYRLLP--ENADQ 58 Query: 85 TSEKQGNIALLTCNLPNGSHFGSLLISGLEKEISTKGYTLSIYFVRENDINGMTLPGNFE 144 + + +IALLT +P +H+GS IS +S GYTL IY ++ ++ LP NF Sbjct: 59 PAVQGRSIALLTLCMPGRNHYGSNFISSFANRMSRAGYTLMIYEIQPDEHRDCRLPANFV 118 Query: 145 PSSVDGIICIEMFNKAYSKLITDLGIPTIFIDCAADMVYPELKADLVLMENEHSTYSVTR 204 + GI+CIEMF++ Y++ I LG+P IF D A + + D + MEN S+ ++T Sbjct: 119 LENTAGILCIEMFDRRYTEFIGRLGLPVIFSDTYARADFSAMAGDFISMENTTSSIAMTN 178 Query: 205 KLIDHGYTSFGFVGDYNHCKSFNERWTGFNRALTECGITLDPAHNIVAPDRNFNINMEEN 264 LI G GFVGD+ HC SF ER+ GF A+ G+TLDP IVAP N E Sbjct: 179 HLIQCGARRLGFVGDHTHCNSFYERFNGFRTAVARAGLTLDPQLCIVAP--NSAPYGETA 236 Query: 265 WMGHQLDALEAWPSVFICANDFIAISVMKSLKNKGIKVPEEVAVCGFDDASESRVVEPHL 324 W+ QL A+ P F+CAND++A+ +MK+LK+ +++PE++ V GFDD+ E+ V EP L Sbjct: 237 WLVEQLQAMPRLPDAFVCANDYLAVHLMKALKHLQLRIPEDIMVAGFDDSPEATVTEPPL 296 Query: 325 TTVHIYSSQMGIISAEILLSRIKDNTKPYQVTHVATDMIFRGST 368 TTV I S +MG I+A ILL RI+ PY+ ++ T ++R +T Sbjct: 297 TTVRIASGEMGFIAAGILLDRIQRPALPYRCCYIQTTPVWRATT 340 >C5EMA5.1 C5EMA5_9FIRM Regulatory protein Length = 344 Score = 200 bits (509), Expect = 1e-49, Method: Compositional matrix adjust. Identities = 115/341 (33%), Positives = 184/341 (53%), Gaps = 5/341 (1%) Query: 31 TIQDIADALGISRNTASKALNGVDSIPAETRDKVIKKAVELKYKQFSYMETTIGTSEKQG 90 TIQDIA L ISRNT SKALNG + ET+ + +KA E+ Y+ + +S QG Sbjct: 4 TIQDIAHELNISRNTVSKALNGKGGVSEETKRLIFEKAREMNYRNILNETSVPASSSSQG 63 Query: 91 NIALLTCNLPNGSHFGSLLISGLEKEISTKGYTLSIYFVRENDINGMTLPGNFEPSSVDG 150 I LT + S F ++ G+E ++ GYTL++ + + ++ P +S+ G Sbjct: 64 TILFLTKASADYSEFWVNVMKGIESVLTPCGYTLTLGIITDEELEKKQFPPIVYDTSIQG 123 Query: 151 IICIEMFNKAYSKLITDLGIPTIFIDCAADMVYPELKADLVLMENEHSTYSVTRKLIDHG 210 II +E+ ++ + + + GIP + +D + + D++ MEN+ + + + L D G Sbjct: 124 IIMVEICSREACQTLIETGIPCVTVDMPKEYENLIGRLDIITMENKSNVKRIVKLLADKG 183 Query: 211 YTSFGFVGD---YNHCKSFNERWTGFNRALTECGITLDPAHNIVAPDRNFNINMEENWMG 267 F F GD YN + F ER+ AL E + LD AH+++ N M+ ++ Sbjct: 184 KRRFSFAGDIYSYNVGRGFQERFDALCEALNELELPLDQAHSLLYETN--NQYMDFPYLV 241 Query: 268 HQLDALEAWPSVFICANDFIAISVMKSLKNKGIKVPEEVAVCGFDDASESRVVEPHLTTV 327 ++L ++E P V+IC ND+ AI VM +++ G +PEEVA GFDD ++S P LTT+ Sbjct: 242 NKLKSMEGLPDVYICGNDWTAIQVMHAIQFLGHHIPEEVAFVGFDDINQSAKCNPPLTTI 301 Query: 328 HIYSSQMGIISAEILLSRIKDNTKPYQVTHVATDMIFRGST 368 H Q+GI +A LL+RI+ P + +T++I R ST Sbjct: 302 HTPKEQLGIAAANCLLTRIRTPRTPCVFSQYSTELIIREST 342 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,145,233,152 Number of extensions: 86817485 Number of successful extensions: 234844 Number of sequences better than 1.0e-05: 5983 Number of HSP's gapped: 229231 Number of HSP's successfully gapped: 5986 Length of query: 372 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 235 Effective length of database: 1,770,017,148 Effective search space: 415954029780 Effective search space used: 415954029780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)