BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0003.p (498 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KI94.1 B3KI94_9BACI Levanase EC=3.2.1.65 645 0.0 Q8RLU2.1 Q8RLU2_PAEPO Exo-inulinase 627 e-178 Q9LAL1.1 Q9LAL1_9PSED Exo-inulinase 550 e-154 Q65GW7.1 Q65GW7_BACLD SacC 481 e-134 Q8GI55.1 Q8GI55_BACST Exo-inulinase EC=3.2.1.80 481 e-133 >B3KI94.1 B3KI94_9BACI Levanase EC=3.2.1.65 Length = 491 Score = 645 bits (1664), Expect = 0.0, Method: Compositional matrix adjust. Identities = 302/486 (62%), Positives = 367/486 (75%), Gaps = 3/486 (0%) Query: 1 MPASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKD 60 M + +QNYR YHFSPQ WMNDPNGMVYF GEYHLF+QHHP G T G MHWGHAVS+D Sbjct: 1 MTNTEQQNYRNAYHFSPQKNWMNDPNGMVYFNGEYHLFYQHHPFGTTWGPMHWGHAVSRD 60 Query: 61 LVTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 LV+WEELP+AL PDE G IFSGSAVVDWNN++GFF PGLVAIFTHH D+ Q +Q Sbjct: 61 LVSWEELPIALAPDEHGTIFSGSAVVDWNNSSGFFDGGPGLVAIFTHHHDVQGAQQMIQY 120 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 QSLAYS D GRTWTKY+GNPVL D+++DFRDPKVFW+ + +WVMIVACGQTVCLY S Sbjct: 121 QSLAYSKDEGRTWTKYDGNPVLSHDSYVDFRDPKVFWHEPSSQWVMIVACGQTVCLYRSS 180 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 +L W FASEFG+GIG HDGVWECPDLFPL + ++WVMLVSIG DPA+ EGSRTQ Sbjct: 181 NLKEWVFASEFGEGIGFHDGVWECPDLFPLA--AEDGEVRWVMLVSIGDDPAYVEGSRTQ 238 Query: 241 YFTGDFDGNVFTPDEDSR-TIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQ 299 YF G+FDG F PDE S+ +RWLDYGRDNYAGVSWSD+P ADGRRLFIGWMSNW YA Sbjct: 239 YFIGEFDGTTFMPDERSKGVVRWLDYGRDNYAGVSWSDIPAADGRRLFIGWMSNWKYANL 298 Query: 300 TPAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASLAGL 359 TP + +RGAMTL REL LE+ EG + + Q+PA+ELEA R +LSL + V E LA + Sbjct: 299 TPTDGWRGAMTLVRELKLETLEGEVTVTQQPARELEAHRTELLSLHGIAVNEAAVRLAAV 358 Query: 360 QLDAYEIAAKLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERYPGRH 419 +L++ EI + S G +VR G G+ET+ G DA++ E+YVDR+ SG FH+ +PG+H Sbjct: 359 RLESCEILVRFDAAFSLGLRVRTGLGQETLVGWDAESAEVYVDRSRSGQADFHKDFPGKH 418 Query: 420 SAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSL 479 SA L+ G + +L I+VDR+SVE+F + GQAVITDLI+P P S GI FA+ + S Sbjct: 419 SAQLKTSGSMLELRIFVDRSSVELFADRGQAVITDLIYPDPASTGITVFADDDRKVIESF 478 Query: 480 EVYKLA 485 +Y+++ Sbjct: 479 HIYEVS 484 >Q8RLU2.1 Q8RLU2_PAEPO Exo-inulinase Length = 485 Score = 627 bits (1618), Expect = e-178, Method: Compositional matrix adjust. Identities = 294/484 (60%), Positives = 357/484 (73%) Query: 1 MPASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKD 60 M ++ YR YHFSP++ WMNDPNGMVY+EG YHLF+QHHP G T G MHWGHAVS D Sbjct: 1 MNVIRQEKYRPSYHFSPKSGWMNDPNGMVYYEGRYHLFYQHHPFGTTWGPMHWGHAVSTD 60 Query: 61 LVTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 L+ WEE P+AL PDELG IFSGSAVVD +T+GFF KPGLVAIFTHH +P + Q Sbjct: 61 LMNWEEQPIALEPDELGTIFSGSAVVDEQDTSGFFGGKPGLVAIFTHHASLPGTEQVRQC 120 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 QSLAYS D+G+TW KY GNPVLED IDFRDPKVFW++ T +WVM++ACGQT+ +YHSP Sbjct: 121 QSLAYSTDSGKTWIKYTGNPVLEDKECIDFRDPKVFWHKPTEQWVMVLACGQTIRIYHSP 180 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 +L WTFASEFG GIGSHD VWECPDLFPL++D +KWVMLVSIG P EGSRTQ Sbjct: 181 NLKEWTFASEFGHGIGSHDAVWECPDLFPLYIDEKREQVKWVMLVSIGDVPEIREGSRTQ 240 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQT 300 YFTG+FDG F D +S +RWLDYGRDNYAGV WSD+P DGRRLF+GWMSNW YA QT Sbjct: 241 YFTGEFDGTTFVADAESEKVRWLDYGRDNYAGVCWSDIPAEDGRRLFMGWMSNWRYANQT 300 Query: 301 PAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASLAGLQ 360 P E +RGAM++PREL LE+R+G + LVQ+P +ELE R PVLSL + + +EV +L+ LQ Sbjct: 301 PTERWRGAMSIPRELALETRKGTVALVQRPVRELEGLRTPVLSLTEPSWEEVRNALSALQ 360 Query: 361 LDAYEIAAKLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERYPGRHS 420 LD YE+ A+ GFKVRV +ET+ G + T E+YVDR SG + FHE + G+H Sbjct: 361 LDCYELVAEFATTGDFGFKVRVSDEQETLVGYTSATQEVYVDRKRSGRSDFHEDFAGKHG 420 Query: 421 AALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSLE 480 A+L + D+ IYVDR+S+EVF + GQ ITDLIFP E+ G+ F+ E+ SLE Sbjct: 421 ASLTILPDRMDIRIYVDRSSIEVFFDRGQVAITDLIFPDAEAKGLEIFSAEEQITVFSLE 480 Query: 481 VYKL 484 +Y L Sbjct: 481 LYAL 484 >Q9LAL1.1 Q9LAL1_9PSED Exo-inulinase Length = 501 Score = 550 bits (1417), Expect = e-154, Method: Compositional matrix adjust. Identities = 260/481 (54%), Positives = 328/481 (68%), Gaps = 2/481 (0%) Query: 6 KQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDLVTWE 65 ++ +R +HF+P+ WMNDPNG+VY+EGEYHLF+Q+HP G T G MHWGHAVSKDL+ W+ Sbjct: 7 QEKFRPQFHFTPETNWMNDPNGLVYYEGEYHLFYQYHPFGNTWGPMHWGHAVSKDLIHWD 66 Query: 66 ELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQRQSLAY 125 LP+AL+PD +G IFSGSAV+DW +T+GFF G+VA+FTH P+ P QRQSLAY Sbjct: 67 HLPIALYPDHIGQIFSGSAVIDWQDTSGFFNGGAGMVALFTHADKFPDSGRPRQRQSLAY 126 Query: 126 SHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSPDLIHW 185 S D GRTW KYEGNP D+A DFRDPKVFW++ + KW+MI+A G V +YHS +L W Sbjct: 127 STDRGRTWIKYEGNPFFADEAMTDFRDPKVFWHKASNKWIMILAAGNCVRIYHSSNLKQW 186 Query: 186 TFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQYFTGD 245 FASEFG GSH GVWECPDLF L VDGD + KWVM+VSIG + EGSRTQYF G Sbjct: 187 KFASEFGANEGSHTGVWECPDLFELPVDGDRNRKKWVMVVSIGNSDEYLEGSRTQYFIGQ 246 Query: 246 FDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQTPAEEF 305 FDG FT + D T+ W+D+GRDNYAGV+WSD+PE DGRRLFI WMSNW YA TP E + Sbjct: 247 FDGIRFTNENDPETVLWVDHGRDNYAGVTWSDIPEEDGRRLFIAWMSNWKYANHTPTEIW 306 Query: 306 RGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNASLAGLQLDAYE 365 R AMT+PR L L S L Q P ELE+ R+ L KD+ V L+ + D +E Sbjct: 307 RSAMTIPRSLSLRSTPEGTRLFQTPVTELESLRLEKLEWKDLEVTTNGNVLSNISGDIFE 366 Query: 366 IAA--KLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHERYPGRHSAAL 423 I A +L + GFKVR A +ET+ G DA+ L++DR SG++ F E +P +H A + Sbjct: 367 IIAEFELNTALEFGFKVRKSADQETIVGYDAEQQMLFIDRTQSGVSDFCEHFPCKHGAVM 426 Query: 424 QAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEETPFLSLEVYK 483 +HI+VDR+SVEVFGN G+ +TDLIFP S GI +A+ +SL ++ Sbjct: 427 IPNQNRIQMHIFVDRSSVEVFGNNGELTMTDLIFPDDSSTGIEVYAKEGNVKLVSLMLFP 486 Query: 484 L 484 L Sbjct: 487 L 487 >Q65GW7.1 Q65GW7_BACLD SacC Length = 677 Score = 481 bits (1237), Expect = e-134, Method: Compositional matrix adjust. Identities = 247/491 (50%), Positives = 319/491 (64%), Gaps = 16/491 (3%) Query: 2 PASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKDL 61 P +++R YHF+P+A WMNDPNGMVY+ GEYHLF+Q+HP G+ G MHWGHAVSKDL Sbjct: 27 PDYYNEDHRPKYHFTPEANWMNDPNGMVYYAGEYHLFYQYHPYGLRWGPMHWGHAVSKDL 86 Query: 62 VTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFA--DKPGLVAIFTHHLDMPEGQPPVQ 119 V WE LPVAL+PDE G IFSGSAV D +NTTGF +KP LVAI+T + + Q Sbjct: 87 VKWEHLPVALYPDEKGTIFSGSAVADRHNTTGFQTGTEKP-LVAIYT------QDRSGEQ 139 Query: 120 RQSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHS 179 QS+AYS+D GRTWTKY GNPV+ + DFRDPKV W+ +T KWVM++A G + +Y S Sbjct: 140 VQSIAYSNDKGRTWTKYSGNPVIPNPGKRDFRDPKVIWHEQTKKWVMLLAGGDRILIYTS 199 Query: 180 PDLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRT 239 PDL WT+ASEFG+G GSH GVWECPDLF L V+G + KWVM VS+G D A + GS Sbjct: 200 PDLKRWTYASEFGEGEGSHGGVWECPDLFELPVEGRPNETKWVMQVSVG-DGAVSGGSGM 258 Query: 240 QYFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQ 299 QYF G FDG F + + W DYG+D YA VSWSD+P++DGRRL++GWMSNW YA Sbjct: 259 QYFVGSFDGTTFKNENPPNRVLWTDYGKDFYAAVSWSDIPKSDGRRLWLGWMSNWQYAND 318 Query: 300 TPAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQEVNAS-LAG 358 P +R AM++PRE+ L++ + ++Q P EL++ R + K+ + N + L G Sbjct: 319 VPTSPWRSAMSIPREVKLKAFSEGLRMIQAPVAELQSIRGASQTWKNKIISPRNGNLLKG 378 Query: 359 LQLDAYEIAAKLPRGMSA----GFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHER 414 L DAYEI A+ A GFKVR G E T G + L+VDR+ SG F+ Sbjct: 379 LSGDAYEINAEFQVNTGAAAEFGFKVRTGENEYTKIGYSKNSASLFVDRSQSGNVSFNPN 438 Query: 415 Y-PGRHSAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFAEHEE 473 + G+H+A L+ + IYVDR+SVEVFGN G+ VITD+I P S G+ +A + Sbjct: 439 FNTGKHAAPLEPVAGKVKMRIYVDRSSVEVFGNDGRQVITDIILPDQSSKGLEVYASNGF 498 Query: 474 TPFLSLEVYKL 484 S+ V+ L Sbjct: 499 VKLNSMTVHPL 509 >Q8GI55.1 Q8GI55_BACST Exo-inulinase EC=3.2.1.80 Length = 493 Score = 481 bits (1237), Expect = e-133, Method: Compositional matrix adjust. Identities = 231/476 (48%), Positives = 305/476 (64%), Gaps = 14/476 (2%) Query: 1 MPASTKQNYRGVYHFSPQAKWMNDPNGMVYFEGEYHLFFQHHPDGMTMGAMHWGHAVSKD 60 M + YR +HFSP+ WMNDPNG+VYF+GEYHLFFQH+P+ G MHWGHAVSKD Sbjct: 1 MKTHNSEKYRPTFHFSPKKNWMNDPNGLVYFKGEYHLFFQHNPNDSIWGPMHWGHAVSKD 60 Query: 61 LVTWEELPVALFPDELGMIFSGSAVVDWNNTTGFFADKPGLVAIFTHHLDMPEGQPPVQR 120 ++ WEEL +AL+PDE G IFSGS V+DWNNT+GFF +PG+VAIFT +L + P+Q Sbjct: 61 MIEWEELDIALYPDENGTIFSGSVVIDWNNTSGFFPKEPGMVAIFTQNLHDADHTTPIQT 120 Query: 121 QSLAYSHDNGRTWTKYEGNPVLEDDAFIDFRDPKVFWNRETGKWVMIVACGQTVCLYHSP 180 QSLA+SHD GRTWTKYE NPVL+ +DFRDPKVFW+ E+ KW+M++A GQTV +Y SP Sbjct: 121 QSLAFSHDRGRTWTKYEKNPVLKHPTKVDFRDPKVFWHYESEKWIMVLATGQTVSIYSSP 180 Query: 181 DLIHWTFASEFGKGIGSHDGVWECPDLFPLHVDGDTSNIKWVMLVSIGADPAFAEGSRTQ 240 +LI W F SEFG+ IG HDGVWECPDLF L V+ ++ KWVM VSIG + F GSRTQ Sbjct: 181 NLIDWQFESEFGENIGCHDGVWECPDLFELPVE-NSEEKKWVMFVSIGDNRQFDSGSRTQ 239 Query: 241 YFTGDFDGNVFTPDEDSRTIRWLDYGRDNYAGVSWSDVPEADGRRLFIGWMSNWMYAQQT 300 YF G FDG+ F DE++ + WLD+G+DNYAGV++SD+P+ DGRR++IGWMSNW YA + Sbjct: 240 YFIGSFDGSNFKVDENNNEVLWLDFGKDNYAGVTFSDIPKEDGRRIYIGWMSNWRYANEV 299 Query: 301 PAEEFRGAMTLPRELYLESREGNILLVQKPAQELEAARIPVLSLKDVTVQ-------EVN 353 P +RG MTLPR L L + ++Q P +EL+ + + D + ++N Sbjct: 300 PTVGWRGQMTLPRVLSLRKIGDKLRVIQNPVKELDNYFTKQIVIHDEIISSGIQKKIDIN 359 Query: 354 ASLAGLQLDAYEIAAKLPRGMSAGFKVRVGAGEETVTGIDAQTGELYVDRAASGLTGFHE 413 + L+ + AK G + + T ID T L +DR SG F E Sbjct: 360 QPSVEIVLNVNNLGAK-----QFGLVLHHTENQSTTITIDLLTNLLSLDRTNSGEVSFSE 414 Query: 414 RYPGRHSAALQAPGEIHDLHIYVDRTSVEVFGNGGQAVITDLIFPKPESAGIAAFA 469 + + ++ +L I VD +SVE+F N G +T LI+P I+ F+ Sbjct: 415 NFLNNQVMKIHDTTKL-NLRIIVDSSSVEIFVNEGAHALTSLIYPDKACEKISIFS 469 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,466,341,870 Number of extensions: 164409737 Number of successful extensions: 315313 Number of sequences better than 1.0e-05: 1269 Number of HSP's gapped: 311053 Number of HSP's successfully gapped: 1348 Length of query: 498 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 358 Effective length of database: 1,741,754,103 Effective search space: 623547968874 Effective search space used: 623547968874 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)