BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0002.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DFB9.1 B1DFB9_9BACL ROK family protein 363 1e-98 B3K646.1 B3K646_9BACI ROK family protein 356 2e-96 B3KI95.1 B3KI95_9BACI ROK family protein 344 7e-93 C3WBR3.1 C3WBR3_FUSMR Fructokinase 325 3e-87 C3W9R5.1 C3W9R5_FUSMR Fructokinase 318 3e-85 >B1DFB9.1 B1DFB9_9BACL ROK family protein Length = 293 Score = 363 bits (932), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 172/268 (64%), Positives = 203/268 (75%) Query: 10 MRIGAIEAGGTKFVLGIGDEKGEILDRLSLPTGHPDQTLPQVIEYFQDKEVEAIGVGSFG 69 MRIGAIEAGGTKFV GIG+E GEI +R+SLPT HPD TL +V+ YF+DK+VEAIG+G+FG Sbjct: 1 MRIGAIEAGGTKFVCGIGNEHGEIEERISLPTEHPDSTLREVLNYFRDKQVEAIGIGTFG 60 Query: 70 PIDINPESPTYGCVTSTPKPGWENYDMLGTLRHAFPVPFGWDTDVNXXXXXXXXXXXXQG 129 PIDI+P SPTYG VT+TPKPGW YD LGT++ PVP+GWDTDVN +G Sbjct: 61 PIDIDPSSPTYGYVTTTPKPGWSGYDFLGTMKRELPVPYGWDTDVNAAAFGEAKWGAAKG 120 Query: 130 LSSCLXXXXXXXXXXXXXSEGTIIHGLVHPEGGHVLTRRHPEDKFAGVCPYHGDCLEGMA 189 L+S L +EG ++HGL+HPEGGHVLTRRHPED FAG CPYHGDCLEGMA Sbjct: 121 LNSALYYTVGTGIGVGVYTEGKLVHGLLHPEGGHVLTRRHPEDTFAGFCPYHGDCLEGMA 180 Query: 190 AGPAIEARWGKKGHELPLDHAAWEMEAFYIAQSITSAILLLSPHKIILGGGVMQQQQLFP 249 AGPAI RWG K EL DH AW+MEAFY+ Q+I+SAILLLSP K+ILGGGVM Q+QLFP Sbjct: 181 AGPAIGQRWGVKADELTKDHPAWQMEAFYLGQAISSAILLLSPKKVILGGGVMHQEQLFP 240 Query: 250 LIRQAVLRNLNGYVRSSSLLDHMDEYIV 277 L+R V +NLNGY +L + +D YIV Sbjct: 241 LVRAEVQKNLNGYASHEALREGIDSYIV 268 >B3K646.1 B3K646_9BACI ROK family protein Length = 304 Score = 356 bits (913), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 169/268 (63%), Positives = 203/268 (75%) Query: 10 MRIGAIEAGGTKFVLGIGDEKGEILDRLSLPTGHPDQTLPQVIEYFQDKEVEAIGVGSFG 69 MRIGAIEAGGTKF+ GIG+E+GEILDR+S PT P+ TL QV+ YF+DK++EAIGVGSFG Sbjct: 1 MRIGAIEAGGTKFICGIGNEEGEILDRISFPTDIPEPTLEQVVAYFRDKDIEAIGVGSFG 60 Query: 70 PIDINPESPTYGCVTSTPKPGWENYDMLGTLRHAFPVPFGWDTDVNXXXXXXXXXXXXQG 129 PID++ S TYG VT+TPKPGW +D +G ++ FPVP G+DTDVN +G Sbjct: 61 PIDLDRSSLTYGQVTTTPKPGWSGFDFVGFMKRNFPVPIGFDTDVNAAVFGEAMWGAAKG 120 Query: 130 LSSCLXXXXXXXXXXXXXSEGTIIHGLVHPEGGHVLTRRHPEDKFAGVCPYHGDCLEGMA 189 L SC+ +EG +IHGLVHPEGGHVLTRRHPED F G CPYHGDCLEGMA Sbjct: 121 LDSCVYYTIGTGVGVGVFAEGNLIHGLVHPEGGHVLTRRHPEDPFEGFCPYHGDCLEGMA 180 Query: 190 AGPAIEARWGKKGHELPLDHAAWEMEAFYIAQSITSAILLLSPHKIILGGGVMQQQQLFP 249 +GPAIE RWG KG ELP DH AWEME FY+AQ+I+ ILLLSP K+ILGGGVM Q+QL+P Sbjct: 181 SGPAIEKRWGLKGSELPEDHLAWEMEGFYLAQAISGVILLLSPKKVILGGGVMHQKQLYP 240 Query: 250 LIRQAVLRNLNGYVRSSSLLDHMDEYIV 277 LIR+ V + LNGYV S ++LD ++ YIV Sbjct: 241 LIRKKVAKFLNGYVASPAILDDLENYIV 268 >B3KI95.1 B3KI95_9BACI ROK family protein Length = 294 Score = 344 bits (882), Expect = 7e-93, Method: Compositional matrix adjust. Identities = 164/268 (61%), Positives = 197/268 (73%) Query: 10 MRIGAIEAGGTKFVLGIGDEKGEILDRLSLPTGHPDQTLPQVIEYFQDKEVEAIGVGSFG 69 MRIGA+EAGGTKFV GIG+E G + DR+S PTG P+ TL + I YF + EVEAIG+GSFG Sbjct: 1 MRIGAMEAGGTKFVCGIGNENGIVEDRISFPTGQPEGTLSRAISYFHEHEVEAIGIGSFG 60 Query: 70 PIDINPESPTYGCVTSTPKPGWENYDMLGTLRHAFPVPFGWDTDVNXXXXXXXXXXXXQG 129 PID+ P+SPTYG VTSTPK GW D+LG ++ +P+GWDTDVN +G Sbjct: 61 PIDLKPDSPTYGYVTSTPKHGWSQIDVLGNMKSHMDIPYGWDTDVNAAALGEATWGAAKG 120 Query: 130 LSSCLXXXXXXXXXXXXXSEGTIIHGLVHPEGGHVLTRRHPEDKFAGVCPYHGDCLEGMA 189 L+SC+ +EG ++HGLVHPEGGHV +RHPED FAG CPYHGDCLEG+A Sbjct: 121 LNSCVYYTVGTGIGLGLYAEGRLVHGLVHPEGGHVPVKRHPEDAFAGTCPYHGDCLEGLA 180 Query: 190 AGPAIEARWGKKGHELPLDHAAWEMEAFYIAQSITSAILLLSPHKIILGGGVMQQQQLFP 249 AGPA+EARWG KGH LP DH AWE+EA+YIAQSIT IL SP KIILGGGVMQQ QL+P Sbjct: 181 AGPALEARWGLKGHLLPADHPAWEIEAYYIAQSITGLILTNSPEKIILGGGVMQQAQLYP 240 Query: 250 LIRQAVLRNLNGYVRSSSLLDHMDEYIV 277 LIR V RNLNGYV + ++ +++YIV Sbjct: 241 LIRAEVRRNLNGYVLADEIVHDIEKYIV 268 >C3WBR3.1 C3WBR3_FUSMR Fructokinase Length = 286 Score = 325 bits (833), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 149/268 (55%), Positives = 194/268 (72%) Query: 10 MRIGAIEAGGTKFVLGIGDEKGEILDRLSLPTGHPDQTLPQVIEYFQDKEVEAIGVGSFG 69 M IGA+EAGGTKFV G+G+EKGEI +R+S PT P+ TL +V+E+F+DK +E +G+GSFG Sbjct: 1 MIIGAVEAGGTKFVCGVGNEKGEIFERVSFPTETPEITLAKVVEFFKDKNIERLGIGSFG 60 Query: 70 PIDINPESPTYGCVTSTPKPGWENYDMLGTLRHAFPVPFGWDTDVNXXXXXXXXXXXXQG 129 PID+NP+S TYG +T TPK W + D++G L+ F +P +DTDVN +G Sbjct: 61 PIDVNPKSKTYGYITKTPKLAWTDCDVVGYLKKYFDIPMYFDTDVNGAALGEATWGAAKG 120 Query: 130 LSSCLXXXXXXXXXXXXXSEGTIIHGLVHPEGGHVLTRRHPEDKFAGVCPYHGDCLEGMA 189 L +CL G ++HG++HPE GH+L +RHPEDK+ G CP+H DCLEGMA Sbjct: 121 LDNCLYMTIGTGIGGGALVSGKLVHGMLHPEMGHILVKRHPEDKYEGKCPFHKDCLEGMA 180 Query: 190 AGPAIEARWGKKGHELPLDHAAWEMEAFYIAQSITSAILLLSPHKIILGGGVMQQQQLFP 249 AGPAIEARWG KGHELPLDH AW++EA+YIAQ + + IL LSP KIILGGGVM+Q QLFP Sbjct: 181 AGPAIEARWGIKGHELPLDHVAWDIEAYYIAQGLVNFILTLSPEKIILGGGVMKQSQLFP 240 Query: 250 LIRQAVLRNLNGYVRSSSLLDHMDEYIV 277 IR V+ L GYV++ +L+++D YIV Sbjct: 241 KIRANVIELLKGYVQTKEILENIDNYIV 268 >C3W9R5.1 C3W9R5_FUSMR Fructokinase Length = 287 Score = 318 bits (815), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 144/268 (53%), Positives = 191/268 (71%) Query: 10 MRIGAIEAGGTKFVLGIGDEKGEILDRLSLPTGHPDQTLPQVIEYFQDKEVEAIGVGSFG 69 MRIGAIEAGGTKF+ G+G+EKGEI +++S PT P+ TL +V+E+F+DK +E +G+G FG Sbjct: 1 MRIGAIEAGGTKFICGVGNEKGEIFEKVSFPTETPEITLAKVVEFFKDKNIERLGIGFFG 60 Query: 70 PIDINPESPTYGCVTSTPKPGWENYDMLGTLRHAFPVPFGWDTDVNXXXXXXXXXXXXQG 129 PID+NP+S TYG +T TPK W + D++G L+ F +P +DTDVN +G Sbjct: 61 PIDVNPKSKTYGYITKTPKLAWTDCDIVGYLKKYFDIPMFFDTDVNGAALGEATWGAAKG 120 Query: 130 LSSCLXXXXXXXXXXXXXSEGTIIHGLVHPEGGHVLTRRHPEDKFAGVCPYHGDCLEGMA 189 L +CL G ++HG++HPE GH+L +RHPED + G CP+H DC EGMA Sbjct: 121 LENCLYITIGTGIGGGAVVSGKLVHGMLHPEMGHILVKRHPEDTYEGKCPFHKDCFEGMA 180 Query: 190 AGPAIEARWGKKGHELPLDHAAWEMEAFYIAQSITSAILLLSPHKIILGGGVMQQQQLFP 249 AGPAIEARWG KGHELP+DH AW++EA+YIAQ + + IL LSP KIILGGGVM+Q QLFP Sbjct: 181 AGPAIEARWGVKGHELPVDHKAWDIEAYYIAQGVVNFILTLSPEKIILGGGVMKQSQLFP 240 Query: 250 LIRQAVLRNLNGYVRSSSLLDHMDEYIV 277 IR+ V R L GY+++ +L+ + YIV Sbjct: 241 KIRKEVKRLLKGYIQTKEILEDIGNYIV 268 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.139 0.440 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,724,591,031 Number of extensions: 70038345 Number of successful extensions: 134234 Number of sequences better than 1.0e-05: 283 Number of HSP's gapped: 133919 Number of HSP's successfully gapped: 290 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)