BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0532.0008.p (284 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B8FVA8.1 B8FVA8_DESHD Nitroreductase 258 3e-67 C0C9Q4.1 C0C9Q4_9FIRM Putative uncharacterized protein 251 5e-65 A6TS08.1 A6TS08_ALKMQ Nitroreductase 248 4e-64 Q24XI3.1 Q24XI3_DESHY Putative uncharacterized protein 242 3e-62 B2A433.1 B2A433_NATTJ Nitroreductase 242 3e-62 >B8FVA8.1 B8FVA8_DESHD Nitroreductase Length = 287 Score = 258 bits (660), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 131/282 (46%), Positives = 178/282 (63%), Gaps = 8/282 (2%) Query: 7 LPLEETIKTRSSVRTYSRESIEQGTKEQIQWFIGTLGNPFSAEVHFKLLQTE--QAANAR 64 + + + +K R SVRTY E + KE++ PF V F+ ++ E Q Sbjct: 8 MSMIQQMKKRISVRTYKEEFLHPELKEKLNLAFKDNQGPFGGAVRFEFVERELAQKEGNI 67 Query: 65 KLGTYGVIKGAKDYIGASVTNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFA 124 KLGTYG+IKGA Y+ A++ G LE GY E L+LYATSLGLGTCW+GGT+ ++EF+ Sbjct: 68 KLGTYGIIKGASSYVVAAIPEGKRDLEDFGYVLEKLILYATSLGLGTCWLGGTYTKNEFS 127 Query: 125 KAMDVREGQLFPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAE 184 KA+ ++ G++ P I+PIGY KR L E+ +R A S R PW+ LFF ++ PLT AE Sbjct: 128 KAIGLQAGEIMPCITPIGYPHQKRSLLESAMRLAAGSKNRKPWQELFFNQALGRPLTEAE 187 Query: 185 AGDYAYPLEMTRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGIA 244 A +YA PLEM RLAPSASNKQPWRII A G+ HFY + + Y LG+DIQ+VD+GIA Sbjct: 188 ADNYATPLEMVRLAPSASNKQPWRIIM-AQGRA-HFYLQRTKDYGKGLGFDIQRVDMGIA 245 Query: 245 ACHFQLAAEDKNLPGAFRVLPLPL----TNPPEHLHYLFSWT 282 CHF+LAA++ LPG ++++ + P HY+ SW Sbjct: 246 MCHFELAAKELGLPGQWQIIESKADAAESTIPPGTHYIVSWV 287 >C0C9Q4.1 C0C9Q4_9FIRM Putative uncharacterized protein Length = 275 Score = 251 bits (642), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 130/271 (47%), Positives = 166/271 (61%), Gaps = 3/271 (1%) Query: 11 ETIKTRSSVRTYSRESIEQGTKEQIQWFIGTLGNPFSAEVHFKLLQTEQAANARKLGTYG 70 +TI+ R SVRTY ++ + + F+ L NPF VH +L + KLGTYG Sbjct: 2 KTIQKRHSVRTYEERALSPQDRAALVAFMERLDNPFGVPVHEYILDRKVDTAGEKLGTYG 61 Query: 71 VIKGAKDYIGASVTNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFAKAMDVR 130 VIKGA ++G SV LA A GYEFE+L+LYAT LGLGT W+ TF R FA +M + Sbjct: 62 VIKGASTFLGVSVPKNELAPLAAGYEFENLLLYATHLGLGTVWLAATFQREHFAASMGIS 121 Query: 131 EGQLFPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAEAGDYAY 190 + +LFP ISP+GY A KR L E ++R KS R PWE LFF+E F+ PLT AEAG YA Sbjct: 122 KEELFPAISPVGYPAAKRSLAETLMRATMKSSVRKPWEELFFQEDFHTPLTTAEAGLYAQ 181 Query: 191 PLEMTRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGIAACHFQL 250 PLEM RLAPSA+N QPWR+ + D YHFY G ++ I++VDLGIA HF Sbjct: 182 PLEMVRLAPSATNAQPWRVCKAGD--LYHFYASYKPGLAEPEQL-IKQVDLGIALSHFHQ 238 Query: 251 AAEDKNLPGAFRVLPLPLTNPPEHLHYLFSW 281 A + L GAF +P + P+ +HY+ SW Sbjct: 239 TALELGLAGAFVSVPQANRDLPDTMHYILSW 269 >A6TS08.1 A6TS08_ALKMQ Nitroreductase Length = 294 Score = 248 bits (633), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 117/255 (45%), Positives = 168/255 (65%), Gaps = 5/255 (1%) Query: 8 PLEETIKTRSSVRTYSRESIEQGTKEQIQWFIGTLGNPFSAEVHFKLLQTEQAA--NARK 65 P+ E IK R SVRTY + + K +I FI PF+ +V F+L+ A +K Sbjct: 10 PITEVIKRRISVRTYEPQPLSVENKRKIMEFIDDTTGPFTPKVRFELIDDLSVAENTGKK 69 Query: 66 LGTYGVIKGAKDYIGASVTNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFAK 125 +GTYGVI+GAK YI +V G LE +GY E ++L+ATSLGLGTCW+GGTF R F + Sbjct: 70 IGTYGVIRGAKTYILVAVERGDGDLEQVGYILEKVILFATSLGLGTCWLGGTFKREGFER 129 Query: 126 AMDVREGQLFPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAE- 184 +++++ + PVI+PIGY K+ L +NIVR A S++R+ WE LF++E+ + PL E Sbjct: 130 LLELKDQEYMPVITPIGYPQNKKSLIDNIVRFAAGSNKRLKWEQLFYKETLDTPLLKEEI 189 Query: 185 AGDYAYPLEMTRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGIA 244 + YA LEM R+APSASNKQPWR++++ D +HFY + +GY+ LG+DIQ++D+GIA Sbjct: 190 SQQYAIALEMVRMAPSASNKQPWRVVKEKD--QFHFYLQRNKGYNKTLGFDIQRIDIGIA 247 Query: 245 ACHFQLAAEDKNLPG 259 CHF++ + L G Sbjct: 248 MCHFEMTINNHGLTG 262 >Q24XI3.1 Q24XI3_DESHY Putative uncharacterized protein Length = 256 Score = 242 bits (617), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 123/257 (47%), Positives = 159/257 (61%), Gaps = 8/257 (3%) Query: 32 KEQIQWFIGTLGNPFSAEVHFKLLQTE--QAANARKLGTYGVIKGAKDYIGASVTNGALA 89 KE+I PF V F+ ++ E Q KLGTYGVIKGA YI ++ G Sbjct: 2 KEKINLAFKDNQGPFGGAVRFEFVERELAQKEGNIKLGTYGVIKGASSYIVTAIPEGKRD 61 Query: 90 LEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEFAKAMDVREGQLFPVISPIGYAAGKRR 149 LE GY E L+LYAT LGLGTCW+GGT+ ++EF KA+ ++ G++ P I+PIGY KR Sbjct: 62 LEDFGYVLEKLILYATYLGLGTCWLGGTYTKNEFGKAIGLQVGEIMPCITPIGYPHQKRS 121 Query: 150 LTENIVRKVAKSDQRMPWETLFFRESFNNPLTPAEAGDYAYPLEMTRLAPSASNKQPWRI 209 L E+ +R A S R PW+ LFF ++ PLT AEA YA PLEM RLAPSASNKQPWRI Sbjct: 122 LLESAMRLAAGSKNRKPWQELFFNQALGRPLTEAEADKYATPLEMVRLAPSASNKQPWRI 181 Query: 210 IRDADGQTYHFYEYKARGYSDALGYDIQKVDLGIAACHFQLAAEDKNLPGAFRVLPLPL- 268 I G HFY + + Y LG+DIQ+VD+GIA CHF+LAA++ LPG ++++ Sbjct: 182 IMAQGG--IHFYLQRTKDYGKGLGFDIQRVDMGIAMCHFELAAKELGLPGQWQIIESKAD 239 Query: 269 ---TNPPEHLHYLFSWT 282 + P HY+ SW Sbjct: 240 AAESTIPPGTHYIVSWV 256 >B2A433.1 B2A433_NATTJ Nitroreductase Length = 287 Score = 242 bits (617), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 121/279 (43%), Positives = 174/279 (62%), Gaps = 6/279 (2%) Query: 7 LPLEETIKTRSSVRTYSRESIEQGTKEQIQWFIGTLGNPFSAEVHFKLLQ--TEQAANAR 64 +P+ + IK R+SVRTY +I + + +++ + L +PFS+ V F + T Sbjct: 8 IPINDLIKKRTSVRTYKPAAISEELENKLKIYSDNLHDPFSSSVRFVFINDATFVKETGG 67 Query: 65 KLGTYGVIKGAKDYIGASV-TNGALALEALGYEFEHLVLYATSLGLGTCWMGGTFNRSEF 123 K+GTYG+IKGAK++I V N LE +GY E L+LYAT LGLGTCW+GGTFN Sbjct: 68 KIGTYGLIKGAKNFIAVLVEKNLNHNLEKVGYTLEKLILYATYLGLGTCWLGGTFNLKGL 127 Query: 124 AKAMDVREGQLFPVISPIGYAAGKRRLTENIVRKVAKSDQRMPWETLFFRESFNNPLTPA 183 KA + E ++ P+I+P+GY K+ + + +++ A S +R PW LFF +S + P+T Sbjct: 128 DKATMITEQEILPIITPVGYPHEKKSIADKMIKAAAGSKKRKPWNKLFFNKSLDTPITEK 187 Query: 184 EAGDYAYPLEMTRLAPSASNKQPWRIIRDADGQTYHFYEYKARGYSDALGYDIQKVDLGI 243 EAG +A PLEM RLAPSA NKQPWRII+ + + +HF+ ++ + GYDIQK+DLGI Sbjct: 188 EAGSFAAPLEMVRLAPSAQNKQPWRIIK--EDKYWHFFLEYSKLINKVSGYDIQKIDLGI 245 Query: 244 AACHFQLAAEDKNLPGAFRVL-PLPLTNPPEHLHYLFSW 281 A CHF+LAA + L G + + P E+ HY+ SW Sbjct: 246 AMCHFELAAVQEGLSGKWMIDGTRPKIEGTENFHYITSW 284 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,730,849,798 Number of extensions: 72458037 Number of successful extensions: 160777 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 160742 Number of HSP's successfully gapped: 50 Length of query: 284 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 150 Effective length of database: 1,798,280,193 Effective search space: 269742028950 Effective search space used: 269742028950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)