BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0531.0007.p (433 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A3DBX2.1 A3DBX2_CLOTH Transcriptional regulator, LacI family 283 2e-74 B4BAW6.1 B4BAW6_CLOTM Transcriptional regulator, LacI family EC=... 283 2e-74 B0KCV8.1 B0KCV8_THEP3 Transcriptional regulator, LacI family 280 1e-73 Q8RCR4.1 Q8RCR4_THETN Transcriptional regulator 278 1e-72 Q9K7X7.1 Q9K7X7_BACHD Transcriptional regulator (LacI family) 276 3e-72 >A3DBX2.1 A3DBX2_CLOTH Transcriptional regulator, LacI family Length = 353 Score = 283 bits (725), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 142/356 (39%), Positives = 230/356 (64%), Gaps = 15/356 (4%) Query: 83 MRSEDIAKLAGVSRSTVSRVINNYSNVPEETRAKVLKVIEQHQYEPNSFARALAGKKTDT 142 M S+DIAK+ GVSRSTVSRVINNY ++P+ TR KVLK I+++ Y PN+ AR LAG K+ T Sbjct: 1 MNSKDIAKIVGVSRSTVSRVINNYPDIPQATREKVLKAIKEYNYYPNASARRLAGMKSST 60 Query: 143 IGLFAISMNEKE--------NATRIYQNNYFAPFVDAVVDTANARGCYVLIHTVYSPDDF 194 +G+F I + + E N +Y N+YF+PF++A +D +N +VL+ T+YS D+ Sbjct: 61 LGIFIIDIKDNEKPHHVIENNEDLLYGNSYFSPFINAFIDQSNKAQYHVLVSTIYSSDEL 120 Query: 195 LKVKQAFLQKRIDGGIIVGTQK-DIDIVREMVNLEFPLVLIDYDISEIMSEHLDRNHLAI 253 K++ AF +KRIDG +I+G+ D + E+++ + V +D D+ E + + Sbjct: 121 WKIQSAFYEKRIDGAVIIGSSSIDYSKIFEIMDKDSITVAVDLDM-----EKENTGTVMS 175 Query: 254 VNSKDYEGTVEAIEYLISLGHEEIGMICGRMNTYSGRERYMAYEDTLKKHGLALRESFIL 313 VN +Y G +AI+YL+ LGH++I +I G +N SG+ R+ +++D L +HGL L FI Sbjct: 176 VNINNYGGVSDAIDYLVELGHKDIAVITGDLNKLSGKIRFESFKDALLRHGLPLNNDFIA 235 Query: 314 QGDFLKETAYEEVTKLLASGAALPTAFFSANDDMAISAMEAFSEHGISVPEDISIAGFDD 373 GDF + + YE + K+LASG PTA F++ND MAI A A E+G+ +PEDIS+ GFD+ Sbjct: 236 YGDFTENSGYEGMKKILASGKK-PTAVFTSNDTMAIGAYRAIKEYGLKIPEDISVMGFDN 294 Query: 374 VQLASRIHPKLTSVRLPIYEMSKAAVEKVIELCDSRQPTFSTISFPARLVQRDSCQ 429 ++ + P LT+V + + E++K ++E +++ ++++ + ++V+R+SC+ Sbjct: 295 SYISQYMSPPLTTVNVSLPEIAKCSIELLLDSINNKEIKNRQKTVNVQIVKRNSCK 350 >B4BAW6.1 B4BAW6_CLOTM Transcriptional regulator, LacI family EC=5.1.1.1 Length = 353 Score = 283 bits (725), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 142/356 (39%), Positives = 230/356 (64%), Gaps = 15/356 (4%) Query: 83 MRSEDIAKLAGVSRSTVSRVINNYSNVPEETRAKVLKVIEQHQYEPNSFARALAGKKTDT 142 M S+DIAK+ GVSRSTVSRVINNY ++P+ TR KVLK I+++ Y PN+ AR LAG K+ T Sbjct: 1 MNSKDIAKIVGVSRSTVSRVINNYPDIPQATREKVLKAIKEYNYYPNASARRLAGMKSST 60 Query: 143 IGLFAISMNEKE--------NATRIYQNNYFAPFVDAVVDTANARGCYVLIHTVYSPDDF 194 +G+F I + + E N +Y N+YF+PF++A +D +N +VL+ T+YS D+ Sbjct: 61 LGIFIIDIKDNEKPHHVIENNEDLLYGNSYFSPFINAFIDQSNKAQYHVLVSTIYSSDEL 120 Query: 195 LKVKQAFLQKRIDGGIIVGTQK-DIDIVREMVNLEFPLVLIDYDISEIMSEHLDRNHLAI 253 K++ AF +KRIDG +I+G+ D + E+++ + V +D D+ E + + Sbjct: 121 WKIQSAFYEKRIDGAVIIGSSSIDYSKIFEIMDKDSITVAVDLDM-----EKENTGTVMS 175 Query: 254 VNSKDYEGTVEAIEYLISLGHEEIGMICGRMNTYSGRERYMAYEDTLKKHGLALRESFIL 313 VN +Y G +AI+YL+ LGH++I +I G +N SG+ R+ +++D L +HGL L FI Sbjct: 176 VNINNYGGVSDAIDYLVELGHKDIAVITGDLNKLSGKIRFESFKDALLRHGLPLNNDFIA 235 Query: 314 QGDFLKETAYEEVTKLLASGAALPTAFFSANDDMAISAMEAFSEHGISVPEDISIAGFDD 373 GDF + + YE + K+LASG PTA F++ND MAI A A E+G+ +PEDIS+ GFD+ Sbjct: 236 YGDFTENSGYEGMKKILASGKK-PTAVFTSNDTMAIGAYRAIKEYGLKIPEDISVMGFDN 294 Query: 374 VQLASRIHPKLTSVRLPIYEMSKAAVEKVIELCDSRQPTFSTISFPARLVQRDSCQ 429 ++ + P LT+V + + E++K ++E +++ ++++ + ++V+R+SC+ Sbjct: 295 SYISQYMSPPLTTVNVSLPEIAKCSIELLLDSINNKEIKNRQKTVNVQIVKRNSCK 350 >B0KCV8.1 B0KCV8_THEP3 Transcriptional regulator, LacI family Length = 361 Score = 280 bits (717), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 144/347 (41%), Positives = 212/347 (61%), Gaps = 4/347 (1%) Query: 83 MRSEDIAKLAGVSRSTVSRVINNYSNVPEETRAKVLKVIEQHQYEPNSFARALAGKKTDT 142 M S+DIA++AGVSRSTVSRVINNY NVP ET+ KVLKVI ++ Y P++ AR L GK + T Sbjct: 1 MNSKDIARIAGVSRSTVSRVINNYPNVPPETKEKVLKVIREYNYVPHASARMLRGKSSKT 60 Query: 143 IGLFAISMNEKENATRIYQNNYFAPFVDAVVDTANARGCYVLIHTVYSPDDFLKVKQAFL 202 IGL + + + + ++Y N YF PF AV+D AN G VL+ TVY D+ KV++ F Sbjct: 61 IGLVIVDLKKNSDIHKVYDNVYFGPFTTAVIDFANQLGYNVLVITVYKTRDYKKVREVFY 120 Query: 203 QKRIDGGIIVGTQKDIDIVREMVNLEFPLVLIDYDISEIMSEHLDRNHLAIVNSKDYEGT 262 K I+G I +G + D ++ ++ +ID +I E + +++ IVN + G Sbjct: 121 NKTINGAIFIGVKNDDPDIKNLIEAGLKTAVIDQEIREDEDDAFNKS--IIVNMDNVNGA 178 Query: 263 VEAIEYLISLGHEEIGMICGRMNTYSGRERYMAYEDTLKKHGLALRESFILQGDFLKETA 322 EA +YLI+LGH EI I G M SG R Y+ LK+ G+ + S +++GDF++E+ Sbjct: 179 YEATKYLINLGHTEIAHITGDMEKLSGFARLEGYKKALKEAGIPFKSSLVVKGDFVEESG 238 Query: 323 YEEVTKLLASGAALPTAFFSANDDMAISAMEAFSEHGISVPEDISIAGFDDVQLASRIHP 382 Y+ KL A PTA F++ND MAI AM A E G VPED+SI GFDD+ ++ + P Sbjct: 239 YKAAYKLFKK--AKPTAIFASNDQMAIGAMHALEEMGFKVPEDVSIIGFDDITISRYLKP 296 Query: 383 KLTSVRLPIYEMSKAAVEKVIELCDSRQPTFSTISFPARLVQRDSCQ 429 LT++ + +M++ AVE +I+ + + P +LV+R+SC+ Sbjct: 297 ALTTMSASLLQMAELAVENLIKSIEEEIAITAKFIVPVKLVERESCK 343 >Q8RCR4.1 Q8RCR4_THETN Transcriptional regulator Length = 361 Score = 278 bits (710), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 142/348 (40%), Positives = 212/348 (60%), Gaps = 4/348 (1%) Query: 82 IMRSEDIAKLAGVSRSTVSRVINNYSNVPEETRAKVLKVIEQHQYEPNSFARALAGKKTD 141 ++ S+DIA++AGVSRSTVSRVINNY NV ET+ KVLK I+++ Y P++ AR L GK + Sbjct: 1 MINSKDIARIAGVSRSTVSRVINNYPNVSPETKEKVLKAIKEYNYVPHASARMLRGKSSK 60 Query: 142 TIGLFAISMNEKENATRIYQNNYFAPFVDAVVDTANARGCYVLIHTVYSPDDFLKVKQAF 201 TIGL + + + ++Y N YF PF AV+D AN G VL+ TVY P D+ KV++ F Sbjct: 61 TIGLVIVDLKRNSDIHKVYDNVYFGPFTTAVIDFANQLGYNVLVITVYKPKDYKKVREVF 120 Query: 202 LQKRIDGGIIVGTQKDIDIVREMVNLEFPLVLIDYDISEIMSEHLDRNHLAIVNSKDYEG 261 K I+G I +G + D ++ ++ +ID +I + E+ N IVN + G Sbjct: 121 YNKTINGAIFIGVKNDDPEIKNLIEAGLKTAVIDQEIRD--DENDPFNKSIIVNMDNVTG 178 Query: 262 TVEAIEYLISLGHEEIGMICGRMNTYSGRERYMAYEDTLKKHGLALRESFILQGDFLKET 321 EA +YLISLGH EI I G M SG R Y+ LK+ G+ + S +++GDF++E+ Sbjct: 179 AYEATKYLISLGHREIAHITGDMEKLSGFARLEGYKKALKEAGIPFKSSLVVKGDFVEES 238 Query: 322 AYEEVTKLLASGAALPTAFFSANDDMAISAMEAFSEHGISVPEDISIAGFDDVQLASRIH 381 Y+ KL A PTA F++ND MAI AM+A E G +P+D+SI GFDD+ ++ + Sbjct: 239 GYKAAYKLFKK--AKPTAIFASNDQMAIGAMQALEEMGYKIPDDVSIIGFDDITISRYLK 296 Query: 382 PKLTSVRLPIYEMSKAAVEKVIELCDSRQPTFSTISFPARLVQRDSCQ 429 P LT++ + + +M++ AVE +I + + P +LV+R+SC+ Sbjct: 297 PALTTMSVSLLQMAELAVENLIRSVEEEIAIKAKFVVPVKLVERESCK 344 >Q9K7X7.1 Q9K7X7_BACHD Transcriptional regulator (LacI family) Length = 358 Score = 276 bits (706), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 147/357 (41%), Positives = 222/357 (62%), Gaps = 16/357 (4%) Query: 82 IMRSEDIAKLAGVSRSTVSRVINNYSNVPEETRAKVLKVIEQHQYEPNSFARALAGKKTD 141 IMRSED+AKL GVSRST++RVINNY ++P+ TR KV K IE++ Y PN+ AR LAG K Sbjct: 3 IMRSEDLAKLIGVSRSTITRVINNYPDIPQATREKVWKAIEKYNYAPNASARKLAGAKNK 62 Query: 142 TIGLFAISMN-------EKENATRIYQNNYFAPFVDAVVDTANARGCYVLIHTVYSPDDF 194 I + + + + + T IY N +FAP ++AV D AN YV+I VYS +D Sbjct: 63 MIAIVVLDVKNDVTHHLKTTDDTLIYNNPFFAPVINAVSDQANKLDYYVIISIVYSKEDL 122 Query: 195 LKVKQAFLQKRIDGGIIVGTQKD-IDIVREMVNLEFPLVLIDYDISEIMSEHLDRNHLAI 253 KV+ F QK I+G I VGTQ+D + + +++ + + +ID SE + H AI Sbjct: 123 KKVESIFNQKMIEGAIFVGTQEDENEFIFKLIKQGYKIAMID------TSEVYSKEHNAI 176 Query: 254 -VNSKDYEGTVEAIEYLISLGHEEIGMICGRMNTYSGRERYMAYEDTLKKHGLALRESFI 312 +N +YEG+ +A+ +L+ LGHE+IG+I G +N S +ER +++TL HGL L E I Sbjct: 177 YINPDNYEGSAKAVNHLVELGHEKIGIITGNLNKLSAKERLDGFKETLSYHGLKLSEDHI 236 Query: 313 LQGDFLKETAYEEVTKLLASGAALPTAFFSANDDMAISAMEAFSEHGISVPEDISIAGFD 372 QGDF +++ YE + +L +G+ PTA F +ND MAI A +A +E G+++PEDISI GFD Sbjct: 237 FQGDFTEDSGYEGIKSIL-NGSNKPTAIFVSNDTMAIGAYKAIAELGLNIPEDISIVGFD 295 Query: 373 DVQLASRIHPKLTSVRLPIYEMSKAAVEKVIELCDSRQPTFSTISFPARLVQRDSCQ 429 + AS + P LT++ + +++K A +I+ + ++ A L++R+SC+ Sbjct: 296 NSFFASYLSPPLTTIDISFVDIAKRATTLLIKSIEGKEQLGIVEKINATLIKRESCK 352 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,318,434,903 Number of extensions: 88848124 Number of successful extensions: 270890 Number of sequences better than 1.0e-05: 8801 Number of HSP's gapped: 257002 Number of HSP's successfully gapped: 8833 Length of query: 433 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 294 Effective length of database: 1,751,175,118 Effective search space: 514845484692 Effective search space used: 514845484692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)