BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0530.0021.p (323 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0FIN8.1 C0FIN8_9CLOT Putative uncharacterized protein 409 e-112 A6M2S6.1 A6M2S6_CLOB8 Sugar isomerase (SIS) 268 4e-70 B5FKM2.1 B5FKM2_SALDC Sugar isomerase 265 4e-69 B5PRW0.1 B5PRW0_SALHA Sugar isomerase 265 4e-69 B5RGS5.1 B5RGS5_SALG2 Putative phosphosugar-binding protein 265 4e-69 >C0FIN8.1 C0FIN8_9CLOT Putative uncharacterized protein Length = 325 Score = 409 bits (1051), Expect = e-112, Method: Compositional matrix adjust. Identities = 188/321 (58%), Positives = 237/321 (73%) Query: 3 IKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFVHA 62 +K+I+ +I+ K+ GGLKH+YFVACGGS+AAIF G YL+Q EA+TFG + Y S+EFVHA Sbjct: 5 VKEIVKEIKEKMDRAGGLKHVYFVACGGSKAAIFPGLYLLQSEAKTFGATTYTSNEFVHA 64 Query: 63 TPAMLDEQCIVVLCSLNATAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVYSNG 122 P LD +C+ V+CSL AT ETV+AV+ AN GA T+AMTG T MA G Y V YSNG Sbjct: 65 VPKGLDNRCVAVICSLKATPETVEAVKTANAAGAVTVAMTGSMETGMAKVGQYVVTYSNG 124 Query: 123 EQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASRFAD 182 + Q+YS NQ++ LRI FE+L QFE Y +Y A+ A+T++D IVA + IA ++AD Sbjct: 125 DDQVYSDSNQANALRIGFELLKQFENYENYDKAMAAYTEIDQIVAEGKEKALPIAQKWAD 184 Query: 183 EYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIPIIL 242 YKD+ IFYV+ASGP Y AY+M CHFMEMQW+HAV +H+GEYFHGPFETTDK +PI+L Sbjct: 185 TYKDEPIFYVLASGPNYGVAYSMCCCHFMEMQWKHAVCLHTGEYFHGPFETTDKQLPIVL 244 Query: 243 FVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERDIVS 302 +SEG TR LDER L FL +YA+ ID KEL I VAE+F V+MIP+ER V+ Sbjct: 245 LMSEGRTRALDERCLKFLRQYAENYITIDFKELNQGRIAPEVAEFFNPVVMIPIERFYVA 304 Query: 303 RMAEIRNHSMNMRRYMWKVEY 323 +MAE+R HSM+ RRYMWKVEY Sbjct: 305 QMAEVRGHSMDERRYMWKVEY 325 >A6M2S6.1 A6M2S6_CLOB8 Sugar isomerase (SIS) Length = 322 Score = 268 bits (686), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 135/324 (41%), Positives = 195/324 (60%), Gaps = 3/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M I++I+ IRS KE+G +K ++ VACGGS ++S Y +Q EAR Y S+EFV Sbjct: 1 MNIREIVGKIRSS-KEDG-IKEVFLVACGGSLVDMYSSQYFLQSEARELRVLSYTSNEFV 58 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 HATP L + +V+ CS T ETV+A ++A E+GA TI +T ++ Y ++Y Sbjct: 59 HATPKSLGKNSVVIACSHGGNTPETVEAAKVAREKGAATITLTHNKDADLIKYADYNILY 118 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 G + +L I+ EIL+Q E Y Y +E V+ +V + + A + Sbjct: 119 EWGNDTSVEDNPMAIILNIAVEILNQTEVYEGYNKFMEGLKIVNDVVKRGQGLVAERAKK 178 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 +A++YK++ I YV+ SG +Y AY + C MEMQW +A +HSGEYFHGPFE TDK Sbjct: 179 YAEKYKNEPITYVLGSGASYGHAYGFSICSLMEMQWMNASAVHSGEYFHGPFEVTDKETL 238 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 +L ++EG TR LDERAL FL +Y +V V+D KELGI++ID SV E+F ++ + Sbjct: 239 YLLLMNEGRTRELDERALKFLNKYGAKVEVVDCKELGISVIDSSVVEFFNPIVFYSILCV 298 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +A++RNH ++ RRYM KVEY Sbjct: 299 YREELAKLRNHDLDTRRYMGKVEY 322 >B5FKM2.1 B5FKM2_SALDC Sugar isomerase Length = 324 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 190/324 (58%), Gaps = 1/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M +K+ +S+I + E GG+KH+Y+VACGGS AA + ++KEA+ +YNS EF+ Sbjct: 1 MGMKETVSNIVTSQAEKGGIKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 60 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 + P L E +VV+ S T ET++A ++A + GA I +T + + + Y Y Sbjct: 61 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWVMDSPLVAHCDYVETY 120 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 + G+ + + L + E+L Q E YAHY + +K++ IV A + A Sbjct: 121 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 180 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA EYKDD++ Y +ASG Y AY + C FMEMQW H+ +HSGE+FHGPFE TD N P Sbjct: 181 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 240 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 + SEG TR +DERAL FL +Y +R+ V+DAKELG++ I +V +YF + V Sbjct: 241 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 300 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +AE R H + RRYMWKVEY Sbjct: 301 YNRALAEARQHPLTTRRYMWKVEY 324 >B5PRW0.1 B5PRW0_SALHA Sugar isomerase Length = 324 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 190/324 (58%), Gaps = 1/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M +K+ +S+I + E GG+KH+Y+VACGGS AA + ++KEA+ +YNS EF+ Sbjct: 1 MGMKETVSNIVTSQAEKGGVKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 60 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 + P L E +VV+ S T ET++A ++A + GA I +T + + + Y Y Sbjct: 61 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWIMDSPLVAHCDYVETY 120 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 + G+ + + L + E+L Q E YAHY + +K++ IV A + A Sbjct: 121 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 180 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA EYKDD++ Y +ASG Y AY + C FMEMQW H+ +HSGE+FHGPFE TD N P Sbjct: 181 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 240 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 + SEG TR +DERAL FL +Y +R+ V+DAKELG++ I +V +YF + V Sbjct: 241 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 300 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +AE R H + RRYMWKVEY Sbjct: 301 YNRALAEARQHPLTTRRYMWKVEY 324 >B5RGS5.1 B5RGS5_SALG2 Putative phosphosugar-binding protein Length = 325 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 133/324 (41%), Positives = 190/324 (58%), Gaps = 1/324 (0%) Query: 1 MEIKDIISDIRSKLKENGGLKHIYFVACGGSQAAIFSGHYLIQKEARTFGTSIYNSSEFV 60 M +K+ +S+I + E GG+KH+Y+VACGGS AA + ++KEA+ +YNS EF+ Sbjct: 2 MGMKETVSNIVTSQAEKGGIKHVYYVACGGSYAAFYPAKAFLEKEAKALTVGLYNSGEFI 61 Query: 61 HATPAMLDEQCIVVLCSLNA-TAETVQAVQLANERGAYTIAMTGFPTTEMATNGMYPVVY 119 + P L E +VV+ S T ET++A ++A + GA I +T + + + Y Y Sbjct: 62 NNPPVALGENAVVVVASHKGNTPETIKAAEIARQHGAPVIGLTWVMDSPLVAHCDYVETY 121 Query: 120 SNGEQQIYSQGNQSSVLRISFEILHQFEAYAHYQAALEAFTKVDSIVASARTYIKRIASR 179 + G+ + + L + E+L Q E YAHY + +K++ IV A + A Sbjct: 122 TFGDGKDIAGEKTMKGLLSAVELLQQTEGYAHYDDFQDGVSKINRIVWRACEQVAERAQA 181 Query: 180 FADEYKDDEIFYVMASGPAYSTAYTMAYCHFMEMQWRHAVPMHSGEYFHGPFETTDKNIP 239 FA EYKDD++ Y +ASG Y AY + C FMEMQW H+ +HSGE+FHGPFE TD N P Sbjct: 182 FAQEYKDDKVIYTVASGAGYGAAYLQSICIFMEMQWIHSACIHSGEFFHGPFEITDANTP 241 Query: 240 IILFVSEGATRPLDERALTFLTRYAQRVTVIDAKELGINIIDDSVAEYFLSVIMIPVERD 299 + SEG TR +DERAL FL +Y +R+ V+DAKELG++ I +V +YF + V Sbjct: 242 FLFQFSEGNTRAVDERALNFLKKYGRRIEVVDAKELGLSTIKTTVIDYFNHSLFNNVYPV 301 Query: 300 IVSRMAEIRNHSMNMRRYMWKVEY 323 +AE R H + RRYMWKVEY Sbjct: 302 YNRALAEARQHPLTTRRYMWKVEY 325 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,801,007,561 Number of extensions: 69654045 Number of successful extensions: 167918 Number of sequences better than 1.0e-05: 336 Number of HSP's gapped: 168119 Number of HSP's successfully gapped: 336 Length of query: 323 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 187 Effective length of database: 1,779,438,163 Effective search space: 332754936481 Effective search space used: 332754936481 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)